LOCUS       RC235317                9471 bp    DNA     circular  SYN 
DEFINITION  SLIT3 (Myc-DDK tagged) - Homo sapiens slit homolog 3 (Drosophila) (SLIT3), transcript variant 1
ACCESSION   NM_001271946
VERSION     NM_001271946.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5628..5719 
                     /label="Myc-DDK"  
     misc_feature    5749..6334 
                     /label="PolyA signal"  
     misc_feature    5806..5825 
                     /label="Restriction Enzyme"  
     misc_feature    6610..7282 
                     /label="ColE1"  
     misc_feature    7712..8506 
                     /label="Kan/Neo"  
     misc_feature    8541..8843 
                     /label="SV40 ori"  
     misc_feature    8929..9406 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5610..5616 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5610 
                     /label="SLIT3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCCCCGGG TGGGCAGGGG TCGGCGCCGC CGTGCGCGCC CGCCTGGCGC 
     1081 TGGCCTTGGC GCTGGCGAGC GTCCTGAGTG GGCCTCCAGC CGTCGCCTGC CCCACCAAGT 
     1141 GTACCTGCTC CGCTGCCAGC GTGGACTGCC ACGGGCTGGG CCTCCGCGCG GTTCCTCGGG 
     1201 GCATCCCCCG CAACGCTGAG CGCCTTGACC TGGACAGAAA TAATATCACC AGGATCACCA 
     1261 AGATGGACTT CGCTGGGCTC AAGAACCTCC GAGTCTTGCA TCTGGAAGAC AACCAGGTCA 
     1321 GCGTCATCGA GAGAGGCGCC TTCCAGGACC TGAAGCAGCT AGAGCGACTG CGCCTGAACA 
     1381 AGAATAAGCT GCAAGTCCTT CCAGAATTGC TTTTCCAGAG CACGCCGAAG CTCACCAGAC 
     1441 TAGATTTGAG TGAAAACCAG ATCCAGGGGA TCCCGAGGAA GGCGTTCCGC GGCATCACCG 
     1501 ATGTGAAGAA CCTGCAACTG GACAACAACC ACATCAGCTG CATTGAAGAT GGAGCCTTCC 
     1561 GAGCGCTGCG CGATTTGGAG ATCCTTACCC TCAACAACAA CAACATCAGT CGCATCCTGG 
     1621 TCACCAGCTT CAACCACATG CCGAAGATCC GAACTCTGCG CCTCCACTCC AACCACCTGT 
     1681 ACTGCGACTG CCACCTGGCC TGGCTCTCGG ATTGGCTGCG ACAGCGACGG ACAGTTGGCC 
     1741 AGTTCACACT CTGCATGGCT CCTGTGCATT TGAGGGGCTT CAACGTGGCG GATGTGCAGA 
     1801 AGAAGGAGTA CGTGTGCCCA GCCCCCCACT CGGAGCCCCC ATCCTGCAAT GCCAACTCCA 
     1861 TCTCCTGCCC TTCGCCCTGC ACGTGCAGCA ATAACATCGT GGACTGTCGA GGAAAGGGCT 
     1921 TGATGGAGAT TCCTGCCAAC TTGCCGGAGG GCATCGTCGA AATACGCCTA GAACAGAACT 
     1981 CCATCAAAGC CATCCCTGCA GGAGCCTTCA CCCAGTACAA GAAACTGAAG CGAATAGACA 
     2041 TCAGCAAGAA TCAGATATCG GATATTGCTC CAGATGCCTT CCAGGGCCTG AAATCACTCA 
     2101 CATCGCTGGT CCTGTATGGG AACAAGATCA CCGAGATTGC CAAGGGACTG TTTGATGGGC 
     2161 TGGTGTCCCT ACAGCTGCTC CTCCTCAATG CCAACAAGAT CAACTGCCTG CGGGTGAACA 
     2221 CGTTTCAGGA CCTGCAGAAC CTCAACTTGC TCTCCCTGTA TGACAACAAG CTGCAGACCA 
     2281 TCAGCAAGGG GCTCTTCGCC CCTCTGCAGT CCATCCAGAC ACTCCACTTA GCCCAAAACC 
     2341 CATTTGTGTG CGACTGCCAC TTGAAGTGGC TGGCCGACTA CCTCCAGGAC AACCCCATCG 
     2401 AGACAAGCGG GGCCCGCTGC AGCAGCCCGC GCCGACTCGC CAACAAGCGC ATCAGCCAGA 
     2461 TCAAGAGCAA GAAGTTCCGC TGCTCAGGCT CCGAGGATTA CCGCAGCAGG TTCAGCAGCG 
     2521 AGTGCTTCAT GGACCTCGTG TGCCCCGAGA AGTGTCGCTG TGAGGGCACG ATTGTGGACT 
     2581 GCTCCAACCA GAAGCTGGTC CGCATCCCAA GCCACCTCCC TGAATATGTC ACCGACCTGC 
     2641 GACTGAATGA CAATGAGGTA TCTGTTCTGG AGGCCACTGG CATCTTCAAG AAGTTGCCCA 
     2701 ACCTGCGGAA AATAAATCTG AGTAACAATA AGATCAAGGA GGTGCGAGAG GGAGCTTTCG 
     2761 ATGGAGCAGC CAGCGTGCAG GAGCTGATGC TGACAGGGAA CCAGCTGGAG ACCGTGCACG 
     2821 GGCGCGTGTT CCGTGGCCTC AGTGGCCTCA AAACCTTGAT GCTGAGGAGT AACTTGATCG 
     2881 GCTGTGTGAG TAATGACACC TTTGCCGGCC TGAGTTCGGT GAGACTGCTG TCCCTCTATG 
     2941 ACAATCGGAT CACCACCATC ACCCCTGGGG CCTTCACCAC GCTTGTCTCC CTGTCCACCA 
     3001 TAAACCTCCT GTCCAACCCC TTCAACTGCA ACTGCCACCT GGCCTGGCTC GGCAAGTGGT 
     3061 TGAGGAAGAG GCGGATCGTC AGTGGGAACC CTAGGTGCCA GAAGCCATTT TTCCTCAAGG 
     3121 AGATTCCCAT CCAGGATGTG GCCATCCAGG ACTTCACCTG TGATGGCAAC GAGGAGAGTA 
     3181 GCTGCCAGCT GAGCCCGCGC TGCCCGGAGC AGTGCACCTG TATGGAGACA GTGGTGCGAT 
     3241 GCAGCAACAA GGGGCTCCGC GCCCTCCCCA GAGGCATGCC CAAGGATGTG ACCGAGCTGT 
     3301 ACCTGGAAGG AAACCACCTA ACAGCCGTGC CCAGAGAGCT GTCCGCCCTC CGACACCTGA 
     3361 CGCTTATTGA CCTGAGCAAC AACAGCATCA GCATGCTGAC CAATTACACC TTCAGTAACA 
     3421 TGTCTCACCT CTCCACTCTG ATCCTGAGCT ACAACCGGCT GAGGTGCATC CCCGTCCACG 
     3481 CCTTCAACGG GCTGCGGTCC CTGCGAGTGC TAACCCTCCA TGGCAATGAC ATTTCCAGCG 
     3541 TTCCTGAAGG CTCCTTCAAC GACCTCACAT CTCTTTCCCA TCTGGCGCTG GGAACCAACC 
     3601 CACTCCACTG TGACTGCAGT CTTCGGTGGC TGTCGGAGTG GGTGAAGGCG GGGTACAAGG 
     3661 AGCCTGGCAT CGCCCGCTGC AGTAGCCCTG AGCCCATGGC TGACAGGCTC CTGCTCACCA 
     3721 CCCCAACCCA CCGCTTCCAG TGCAAAGTAC TCTGGTTTTG CTGCCCAGGG CCAGTGGACA 
     3781 TCAACATTGT GGCCAAATGC AATGCCTGCC TCTCCAGCCC GTGCAAGAAT AACGGGACAT 
     3841 GCACCCAGGA CCCTGTGGAG CTGTACCGCT GTGCCTGCCC CTACAGCTAC AAGGGCAAGG 
     3901 ACTGCACTGT GCCCATCAAC ACCTGCATCC AGAACCCCTG TCAGCATGGA GGCACCTGCC 
     3961 ACCTGAGTGA CAGCCACAAG GATGGGTTCA GCTGCTCCTG CCCTCTGGGC TTTGAGGGGC 
     4021 AGCGGTGTGA GATCAACCCA GATGACTGTG AGGACAACGA CTGCGAAAAC AATGCCACCT 
     4081 GCGTGGACGG GATCAACAAC TACGTGTGTA TCTGTCCGCC TAACTACACA GGTGAGCTAT 
     4141 GCGACGAGGT GATTGACCAC TGTGTGCCTG AGCTGAACCT CTGTCAGCAT GAGGCCAAGT 
     4201 GCATCCCCCT GGACAAAGGA TTCAGCTGCG AGTGTGTCCC TGGCTACAGC GGGAAGCTCT 
     4261 GTGAGACAGA CAATGATGAC TGTGTGGCCC ACAAGTGCCG CCACGGGGCC CAGTGCGTGG 
     4321 ACACAATCAA TGGCTACACA TGCACCTGCC CCCAGGGCTT CAGTGGACCC TTCTGTGAAC 
     4381 ACCCCCCACC CATGGTCCTA CTGCAGACCA GCCCATGCGA CCAGTACGAG TGCCAGAACG 
     4441 GGGCCCAGTG CATCGTGGTG CAGCAGGAGC CCACCTGCCG CTGCCCACCA GGCTTCGCCG 
     4501 GCCCCAGATG CGAGAAGCTC ATCACTGTCA ACTTCGTGGG CAAAGACTCC TACGTGGAAC 
     4561 TGGCCTCCGC CAAGGTCCGA CCCCAGGCCA ACATCTCCCT GCAGGTGGCC ACTGACAAGG 
     4621 ACAACGGCAT CCTTCTCTAC AAAGGAGACA ATGACCCCCT GGCACTGGAG CTGTACCAGG 
     4681 GCCACGTGCG GCTGGTCTAT GACAGCCTGA GTTCCCCTCC AACCACAGTG TACAGTGTGG 
     4741 AGACAGTGAA TGATGGGCAG TTTCACAGTG TGGAGCTGGT GACGCTAAAC CAGACCCTGA 
     4801 ACCTAGTAGT GGACAAAGGA ACTCCAAAGA GCCTGGGGAA GCTCCAGAAG CAGCCAGCAG 
     4861 TGGGCATCAA CAGCCCCCTC TACCTTGGAG GCATCCCCAC CTCCACCGGC CTCTCTGCCT 
     4921 TGCGCCAGGG CACGGACCGG CCTCTAGGCG GCTTCCACGG ATGCATCCAT GAGGTGCGCA 
     4981 TCAACAACGA GCTGCAGGAC TTCAAGGCCC TCCCACCACA GTCCCTGGGG GTGTCACCAG 
     5041 GCTGCAAGTC CTGCACCGTG TGCAAGCACG GCCTGTGCCG CTCCGTGGAG AAGGACAGCG 
     5101 TGGTGTGCGA GTGCCGCCCA GGCTGGACCG GCCCACTCTG CGATCAGGAG GCCCGGGACC 
     5161 CCTGCCTCGG CCACAGATGC CACCATGGAA AATGTGTGGC AACTGGGACC TCATACATGT 
     5221 GCAAGTGTGC CGAGGGCTAT GGAGGGGACT TGTGTGACAA CAAGAATGAC TCTGCCAATG 
     5281 CCTGCTCAGC CTTCAAGTGT CACCATGGGC AGTGCCACAT CTCAGACCAA GGGGAGCCCT 
     5341 ACTGCCTGTG CCAGCCCGGC TTTAGCGGCG AGCACTGCCA ACAAGAGAAT CCGTGCCTGG 
     5401 GACAAGTAGT CCGAGAGGTG ATCCGCCGCC AGAAAGGTTA TGCATCATGT GCCACAGCCT 
     5461 CCAAGGTGCC CATCATGGAA TGTCGTGGGG GCTGTGGGCC CCAGTGCTGC CAGCCCACCC 
     5521 GCAGCAAGCG GCGGAAATAC GTCTTCCAGT GCACGGACGG CTCCTCGTTT GTAGAAGAGG 
     5581 TGGAGAGACA CTTAGAGTGC GGCTGCCTCG CGTGTTCCAC GCGTACGCGG CCGCTCGAGC 
     5641 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     5701 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     5761 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     5821 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     5881 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     5941 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     6001 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     6061 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     6121 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     6181 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     6241 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     6301 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     6361 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     6421 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     6481 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     6541 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     6601 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     6661 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     6721 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     6781 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     6841 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     6901 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     6961 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     7021 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     7081 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     7141 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     7201 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     7261 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     7321 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     7381 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     7441 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     7501 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     7561 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     7621 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     7681 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     7741 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     7801 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     7861 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     7921 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     7981 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     8041 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     8101 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     8161 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     8221 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     8281 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     8341 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     8401 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     8461 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     8521 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     8581 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     8641 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     8701 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     8761 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     8821 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     8881 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     8941 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     9001 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     9061 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     9121 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     9181 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     9241 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     9301 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     9361 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     9421 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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