LOCUS       RC235316                9429 bp    DNA     circular  SYN 
DEFINITION  CUX1 (Myc-DDK tagged) - Homo sapiens cut-like homeobox 1 (CUX1), transcript variant 4
ACCESSION   NM_001202543
VERSION     NM_001202543.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5586..5677 
                     /label="Myc-DDK"  
     misc_feature    5707..6292 
                     /label="PolyA signal"  
     misc_feature    5764..5783 
                     /label="Restriction Enzyme"  
     misc_feature    6568..7240 
                     /label="ColE1"  
     misc_feature    7670..8464 
                     /label="Kan/Neo"  
     misc_feature    8499..8801 
                     /label="SV40 ori"  
     misc_feature    8887..9364 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5568..5574 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5568 
                     /label="CUX1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGCCAAT GTGGGATCGA TGTTTCAATA TTGGAAGCGC TTTGATTTAC 
     1081 AGCAGCTGCA GAGAGAACTC GATGCCACCG CAACGGTATT GGCGAACCGG CAGGATGAAA 
     1141 GTGAGCAGTC CAGAAAGCGG CTTATCGAAC AGAGCCGGGA GTTCAAGAAG AACACTCCAG 
     1201 AGGATTTGCG CAAGCAGGTA GCGCCGCTGC TGAAGAGTTT CCAAGGAGAG ATTGATGCAC 
     1261 TGAGTAAAAG AAGCAAGGAA GCTGAAGCAG CTTTCTTGAA TGTCTACAAA AGATTGATTG 
     1321 ACGTCCCAGA TCCCGTACCA GCTTTGGATC TCGGACAGCA ACTCCAGCTC AAAGTGCAGC 
     1381 GCCTGCACGA TATTGAAACA GAGAACCAGA AACTTAGGGA AACTCTGGAA GAATACAACA 
     1441 AGGAATTTGC TGAAGTGAAA AATCAAGAGG TTACGATAAA AGCACTTAAA GAGAAAATCC 
     1501 GAGAATATGA ACAGACACTG AAGAACCAAG CCGAAACCAT AGCTCTTGAG AAGGAACAGA 
     1561 AGTTACAGAA TGACTTTGCA GAAAAGGAGA GAAAGCTGCA GGAGACACAG ATGTCCACCA 
     1621 CCTCAAAGCT GGAGGAAGCT GAGCATAAGG TTCAGAGCCT ACAAACAGCC CTGGAAAAAA 
     1681 CTCGAACAGA ATTATTTGAC CTGAAAACCA AATACGATGA AGAAACTACT GCAAAGGCCG 
     1741 ACGAGATTGA AATGATCATG ACGGACCTTG AAAGGGCAAA CCAGAGGGCA GAGGTGGCTC 
     1801 AGAGAGAGGC GGAGACCTTA AGGGAACAGC TCTCATCGGC CAATCACTCC CTCCAGCTGG 
     1861 CCTCACAGAT CCAGAAGGCA CCAGACGTGG AGCAGGCCAT AGAGGTGCTG ACCCGCTCCA 
     1921 GCCTAGAAGT TGAGTTGGCC GCCAAGGAGC GGGAGATCGC ACAGCTGGTG GAGGACGTGC 
     1981 AGAGACTCCA GGCCAGCCTC ACCAAGCTGC GGGAGAATTC GGCCAGCCAG ATCTCACAGC 
     2041 TTGAGCAGCA GCTGAGCGCC AAAAACAGCA CACTCAAACA ACTGGAAGAA AAACTCAAAG 
     2101 GCCAGGCTGA CTATGAAGAG GTGAAGAAAG AGCTGAACAT TCTGAAGTCC ATGGAGTTTG 
     2161 CACCGTCCGA GGGCGCTGGG ACACAGGATG CGGCCAAGCC CCTGGAGGTG CTGTTGCTGG 
     2221 AGAAGAACCG CTCGCTGCAG TCCGAGAACG CCGCGCTGCG CATCTCCAAC AGCGACCTGA 
     2281 GCGGGTCAGC CAGGAGGAAA GGGAAAGACC AGCCTGAAAG TCGGCGCCCG GGATCTTTGC 
     2341 CGGCCCCCCC TCCTTCTCAG TTGCCCCGCA ACCCGGGGGA GCAGGCTTCC AATACTAATG 
     2401 GTACACACCA GTTCTCACCA GCGGGGTTAA GTCAAGACTT TTTCAGCTCA TCCCTGGCAA 
     2461 GCCCCAGCCT ACCCCTGGCT TCTACAGGAA AATTTGCACT AAACTCTCTT CTCCAGCGGC 
     2521 AGCTAATGCA GTCCTTCTAC TCCAAGGCTA TGCAGGAAGC CGGAAGCACA AGCATGATTT 
     2581 TTTCAACAGG TCCATACAGC ACAAACTCCA TATCTTCCCA AAGTCCATTA CAACAAAGCC 
     2641 CAGATGTCAA TGGCATGGCC CCATCCCCCA GCCAGTCAGA AAGTGCTGGG AGCGTCTCCG 
     2701 AGGGCGAGGA GATGGACACT GCAGAAATCG CCCGGCAGGT CAAAGAGCAG CTGATTAAGC 
     2761 ACAATATCGG ACAACGTATT TTCGGACATT ATGTGTTGGG ACTGTCTCAA GGGTCCGTGA 
     2821 GCGAGATTCT GGCCCGGCCC AAGCCATGGA ATAAACTGAC TGTTCGTGGC AAGGAGCCAT 
     2881 TTCACAAGAT GAAACAGTTC CTCTCCGATG AGCAGAACAT CCTGGCCCTC CGTAGCATCC 
     2941 AAGGCAGACA AAGAGAGAAT CCAGGCCAGA GCCTGAACAG ACTATTTCAG GAAGTACCGA 
     3001 AACGAAGAAA TGGGTCTGAA GGTAACATCA CCACCCGGAT CCGAGCCTCG GAGACTGGCT 
     3061 CTGATGAAGC CATCAAGTCC ATCCTAGAGC AAGCCAAGAG GGAGCTCCAA GTGCAGAAAA 
     3121 CTGCAGAGCC GGCCCAGCCT TCCTCCGCAT CCGGCAGCGG GAACTCTGAT GACGCCATCC 
     3181 GCTCCATCCT GCAGCAAGCC CGCCGGGAGA TGGAGGCCCA GCAGGCTGCC CTCGACCCTG 
     3241 CCTTAAAGCA GGCACCACTG TCCCAGAGTG ACATCACCAT CCTCACCCCC AAGCTTCTGT 
     3301 CCACCTCGCC CATGCCCACC GTGTCCAGCT ACCCACCTCT CGCCATCTCC CTGAAGAAGC 
     3361 CCTCCGCAGC TCCTGAGGCC GGTGCCTCTG CTCTGCCGAA CCCCCCGGCC CTCAAAAAGG 
     3421 AGGCCCAGGA CGCCCCCGGG CTGGACCCCC AGGGAGCAGC CGATTGTGCA CAAGGGGTCC 
     3481 TGAGACAGGT GAAAAATGAG GTGGGCCGCA GCGGTGCCTG GAAGGACCAC TGGTGGAGCG 
     3541 CGGTGCAGCC GGAGAGAAGA AATGCCGCCT CCTCCGAGGA GGCCAAGGCC GAAGAAACGG 
     3601 GCGGCGGGAA AGAGAAGGGC AGCGGTGGCA GCGGAGGTGG CAGCCAGCCT CGGGCCGAGC 
     3661 GCAGTCAGCT CCAGGGACCC TCGTCGTCAG AGTACTGGAA GGAGTGGCCC AGCGCTGAGT 
     3721 CCCCATACTC CCAGAGCTCA GAGCTGAGTC TGACCGGGGC CAGCCGCAGC GAGACACCAC 
     3781 AGAACAGCCC CCTGCCATCC TCCCCGATCG TGCCCATGTC CAAGCCCACC AAGCCCTCGG 
     3841 TCCCCCCGCT GACCCCCGAG CAGTACGAGG TCTACATGTA CCAGGAGGTG GACACCATCG 
     3901 AGCTCACCCG GCAGGTTAAG GAAAAGCTGG CCAAGAACGG CATCTGCCAG AGAATCTTCG 
     3961 GGGAGAAGGT GCTGGGCCTG TCCCAGGGCA GCGTCAGCGA CATGCTGTCC CGACCGAAGC 
     4021 CATGGAGCAA GCTGACGCAG AAAGGCCGAG AACCCTTCAT CCGGATGCAG CTCTGGCTGA 
     4081 ACGGCGAGCT AGGCCAGGGT GTTCTACCCG TCCAGGGCCA GCAGCAAGGG CCAGTCCTCC 
     4141 ACTCCGTGAC ATCGCTCCAG GACCCGCTGC AGCAGGGCTG TGTGAGCTCA GAAAGCACTC 
     4201 CAAAGACCTC CGCCAGCTGC AGCCCTGCCC CTGAGTCCCC GATGAGTTCC AGTGAGTCGG 
     4261 TGAAGAGCCT GACCGAGCTG GTCCAGCAGC CCTGTCCCCC CATCGAGGCG AGCAAGGACA 
     4321 GCAAGCCACC AGAGCCCAGT GACCCGCCAG CATCCGACTC CCAGCCCACA ACCCCGCTGC 
     4381 CTCTCTCCGG ACACTCGGCC CTCAGCATCC AAGAATTAGT AGCCATGTCC CCGGAGCTGG 
     4441 ACACCTACGG CATAACCAAG CGGGTGAAGG AGGTGCTGAC GGACAACAAC CTCGGCCAGC 
     4501 GCTTATTTGG GGAGACCATC TTAGGGCTCA CCCAAGGCTC TGTCTCTGAC CTCCTTGCCC 
     4561 GCCCCAAACC CTGGCATAAG CTCAGTCTGA AAGGACGAGA GCCCTTCGTC CGGATGCAGC 
     4621 TGTGGCTGAA CGACCCCAAC AATGTGGAGA AGCTGATGGA CATGAAACGG ATGGAGAAGA 
     4681 AAGCCTACAT GAAGCGGCGG CACAGCTCAG TCAGTGACAG CCAGCCCTGC GAACCGCCCT 
     4741 CTGTCGGCAC CGAGTACAGC CAGGGCGCCA GCCCCCAGCC CCAGCACCAG CTGAAGAAAC 
     4801 CCCGGGTGGT GCTGGCTCCG GAGGAGAAGG AGGCGCTGAA ACGAGCGTAT CAGCAAAAGC 
     4861 CATACCCGTC ACCAAAAACC ATCGAAGACC TCGCCACCCA GCTCAACCTG AAAACCAGCA 
     4921 CCGTCATCAA CTGGTTCCAC AACTACAGGT CTCGGATCCG CAGAGAACTG TTCATTGAGG 
     4981 AAATTCAGGC CGGGAGTCAG GGCCAGGCGG GCGCCAGCGA CTCACCCTCG GCCCGCAGCG 
     5041 GCCGGGCGGC GCCCAGCTCG GAGGGCGACA GCTGCGACGG CGTGGAGGCC ACTGAGGGCC 
     5101 CAGGCAGCGC CGACACCGAG GAGCCCAAGT CTCAGGGAGA GGCCGAGCGG GAGGAGGTGC 
     5161 CGCGGCCGGC GGAGCAGACG GAGCCGCCGC CCTCGGGGAC CCCGGGCCCG GACGACGCCC 
     5221 GCGACGACGA CCACGAGGGA GGCCCCGTGG AAGGCCCGGG GCCCCTGCCC AGCCCCGCCT 
     5281 CCGCGACCGC CACCGCCGCG CCCGCGGCCC CCGAGGACGC CGCTACCTCA GCCGCCGCCG 
     5341 CGCCGGGGGA GGGCCCCGCG GCCCCGAGCT CCGCGCCGCC GCCCAGCAAC AGCAGCAGCA 
     5401 GCAGCGCCCC CCGCAGGCCC AGCTCGCTGC AGAGCCTTTT CGGCCTCCCC GAGGCCGCGG 
     5461 GCGCCCGGGA CTCGCGCGAC AACCCCCTGC GCAAGAAGAA GGCCGCGAAC TTGAACAGCA 
     5521 TCATCCACCG CCTGGAGAAG GCCGCCAGCC GGGAGGAACC TATCGAATGG GAGTTCACGC 
     5581 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     5641 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     5701 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     5761 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     5821 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     5881 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     5941 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     6001 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     6061 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     6121 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     6181 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     6241 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     6301 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     6361 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     6421 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     6481 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     6541 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     6601 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     6661 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     6721 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     6781 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     6841 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     6901 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     6961 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     7021 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     7081 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     7141 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     7201 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     7261 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     7321 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     7381 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     7441 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     7501 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     7561 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     7621 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     7681 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     7741 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     7801 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     7861 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     7921 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     7981 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     8041 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     8101 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     8161 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     8221 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     8281 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     8341 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     8401 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     8461 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     8521 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     8581 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     8641 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     8701 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     8761 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8821 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     8881 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     8941 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     9001 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     9061 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     9121 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     9181 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     9241 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     9301 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     9361 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     9421 GCGAATTTT
//