LOCUS       RC235312                9312 bp    DNA     circular  SYN 
DEFINITION  CLASP1 (Myc-DDK tagged) - Homo sapiens cytoplasmic linker associated protein 1 (CLASP1), transcript variant 4
ACCESSION   NM_001207051
VERSION     NM_001207051.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5469..5560 
                     /label="Myc-DDK"  
     misc_feature    5590..6175 
                     /label="PolyA signal"  
     misc_feature    5647..5666 
                     /label="Restriction Enzyme"  
     misc_feature    6451..7123 
                     /label="ColE1"  
     misc_feature    7553..8347 
                     /label="Kan/Neo"  
     misc_feature    8382..8684 
                     /label="SV40 ori"  
     misc_feature    8770..9247 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5451..5457 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5451 
                     /label="CLASP1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCCTCGC ATGGAGTCCT GCCTGGCGCA GGTGTTGCAG AAGGATGTGG 
     1081 GGAAACGATT GCAGGTTGGC CAAGAACTGA TAGACTATTT CTCAGACAAA CAGAAGTCTG 
     1141 CTGACCTTGA GCATGACCAG ACCATGTTAG ATAAACTTGT GGATGGACTT GCTACCTCTT 
     1201 GGGTGAACTC TAGCAATTAC AAGGTGGTTC TGCTGGGCAT GGACATCCTG TCCGCCCTGG 
     1261 TGACCCGGCT GCAGGATCGG TTCAAGGCGC AGATCGGCAC AGTGCTGCCA AGTCTAATAG 
     1321 ACAGACTAGG AGATGCTAAA GACTCTGTGA GGGAGCAGGA CCAAACTCTG CTGCTAAAGA 
     1381 TCATGGATCA AGCTGCTAAT CCCCAGTACG TATGGGACAG AATGCTTGGA GGCTTCAAAC 
     1441 ACAAGAATTT CCGTACTCGA GAAGGCATCT GTCTCTGCCT TATAGCAACA CTCAATGCCT 
     1501 CTGGAGCACA GACTTTAACA CTAAGCAAGA TTGTGCCACA TATATGCAAC TTACTTGGAG 
     1561 ATCCAAACAG CCAGGTTCGA GATGCAGCAA TAAACAGCTT AGTGGAAATT TACAGACATG 
     1621 TAGGAGAACG TGTGAGGGCA GATCTCAGTA AAAAAGGATT GCCACAGTCC CGGTTGAATG 
     1681 TAATTTTTAC AAAATTTGAT GAAGTCCAGA AATCTGGAAA CATGATACAA TCTGCAAATG 
     1741 ATAAAAATTT CGACGATGAA GATTCTGTGG ATGGTAACAG ACCTTCCTCT GCTAGTTCTA 
     1801 CATCATCCAA GGCTCCACCA AGTTCTCGGA GAAACGTTGG AATGGGAACC ACCCGCCGGC 
     1861 TTGGTTCATC CACCCTTGGA TCCAAGTCTT CAGCTGCAAA AGAAGGAGCT GGTGCTGTTG 
     1921 ATGAAGAGGA TTTTATTAAA GCATTTGATG ATGTACCTGT AGTACAGATT TATTCCAGCC 
     1981 GAGACCTTGA GGAATCCATA AACAAAATTA GGGAAATATT ATCTGATGAC AAGCATGATT 
     2041 GGGAGCAGAG AGTAAATGCT CTAAAAAAGA TTAGATCTTT ACTTTTGGCT GGTGCTGCTG 
     2101 AGTATGATAA CTTCTTTCAA CATTTGCGTC TTTTGGATGG AGCCTTTAAA CTCTCTGCTA 
     2161 AGGACCTGCG GTCTCAGGTA GTGCGGGAGG CTTGTATCAC GTTGGGGCAT CTGTCATCAG 
     2221 TTCTGGGGAA TAAGTTTGAC CATGGAGCTG AAGCCATTAT GCCAACTATC TTTAATTTAA 
     2281 TTCCAAACAG TGCCAAAATT ATGGCCACAT CTGGTGTTGT AGCTGTTAGG TTAATTATTC 
     2341 GGCACACACA CATCCCTAGG TTAATACCTG TCATAACAAG CAACTGTACC TCTAAGTCTG 
     2401 TCGCAGTTAG AAGGCGCTGT TTTGAATTTT TAGATTTGCT TTTACAAGAA TGGCAGACAC 
     2461 ATTCACTAGA ACGACACATA TCAGTATTAG CTGAAACAAT AAAGAAGGGA ATACATGATG 
     2521 CTGATTCCGA AGCAAGAATA GAAGCCAGAA AATGTTACTG GGGTTTCCAC AGTCACTTCA 
     2581 GCAGAGAAGC AGAGCACTTG TACCACACCT TGGAGTCCTC CTACCAGAAA GCCCTGCAGT 
     2641 CCCACCTGAA GAACTCAGAC AGCATAGTGT CTCTGCCTCA GTCAGACCGC TCATCTTCCA 
     2701 GCTCTCAAGA GAGTCTAAAT CGTCCGCTGT CTGCCAAAAG AAGTCCTACT GGAAGTACCA 
     2761 CATCTAGAGC TTCTACAGTT AGTACCAAAT CTGTGTCAAC GACTGGGTCC CTCCAGCGAT 
     2821 CTCGAAGTGA TATTGATGTG AACGCAGCAG CCAGTGCCAA ATCCAAAGTC TCCTCATCTT 
     2881 CGGGCACGAC GCCTTTCAGC TCTGCAGCAG CTTTGCCTCC AGGGTCATAC GCATCCTTAG 
     2941 AGTCCAGACA CATGAGGGAA GACATGGAGT ACATAGGCCT GGACTCAGGT CGGATCCGCA 
     3001 CAAGACGGCA AAGCTCTGGG AGTGCCACCA ACGTCGCCTC TACACCTGAT AACCGGGGCC 
     3061 GCAGTCGCGC TAAAGTGGTT TCACAGTCCC AGCCTGGCAG CCGGTCAAGT TCCCCAGGAA 
     3121 AATTGTTGGG AAGTGGTTAT GGTGGACTTA CTGGGGGCTC CTCACGAGGC CCACCTGTGA 
     3181 CACCGTCTTC AGAAAAGCGA AGCAAGATTC CCAGGAGCCA GGGATGTAGC CGGGAAACAA 
     3241 GTCCAAACCG AATAGGATTA GATCGGTTTG GGCTTGGCCA GCCAGGAAGA ATACCTGGTT 
     3301 CTGTGAATGC CATGAGAGTT CTGAGCACAA GTACAGATCT TGAAGCTGCT GTTGCTGATG 
     3361 CTTTGCTGTT AGGAGACTCC AGGAGCAAGA AGCCTGTGAG GAGGAGATAT GAGCCGTATG 
     3421 GGATGTATTC TGACGATGAT GCCAACAGTG ATGCCTCAAG TGTTTGCTCT GAGCGCTCAT 
     3481 ATGGCTCCAG GAATGGTGGC ATTCCCCATT ATCTGCGGCA GACTGAGGAT GTAGCAGAAG 
     3541 TTCTCAACCA CTGTGCTAGT TCAAACTGGT CAGAAAGGAA AGAAGGGCTT CTGGGCCTGC 
     3601 AGAACTTACT GAAGAGCCAA AGAACACTGA GTCGAGTTGA ACTGAAAAGG TTGTGTGAGA 
     3661 TCTTCACTCG GATGTTTGCT GACCCTCATA GCAAGAGAGT TTTCAGTATG TTTTTGGAGA 
     3721 CTCTTGTGGA TTTTATAATA ATTCATAAGG ATGATTTACA AGACTGGCTT TTTGTTCTTC 
     3781 TCACACAATT ACTTAAGAAA ATGGGAGCAG ATTTACTTGG ATCTGTGCAA GCAAAAGTTC 
     3841 AAAAGGCTCT AGATGTCACA AGGGACTCCT TTCCATTTGA TCAACAATTT AACATTTTGA 
     3901 TGAGATTTAT TGTGGATCAA ACTCAAACTC CAAACCTCAA GGTCAAAGTT GCAATCCTGA 
     3961 AATACATTGA GTCTCTGGCC AGACAGATGG ATCCAACAGA TTTTGTAAAC TCTAGTGAGA 
     4021 CAAGGCTTGC TGTTTCTAGA ATCATAACCT GGACAACAGA ACCAAAGAGT TCAGACGTGA 
     4081 GAAAGGCAGC ACAGATTGTG CTAATCTCTC TGTTTGAATT GAATACTCCT GAATTTACCA 
     4141 TGTTACTTGG TGCCTTGCCA AAAACATTCC AGGATGGTGC CACCAAACTC CTGCACAACC 
     4201 ACCTCAAGAA TTCCAGTAAC ACCAGTGTGG GCTCTCCAAG CAATACGATT GGCCGGACGC 
     4261 CCTCCCGACA CACCAGCAGC AGGACCAGCC CCCTGACCTC ACCCACCAAC TGTTCCCATG 
     4321 GGGGTCTGTC TCCAAGCATG CTGGACTATG ATACAGAGAA CCTGAACTCT GAAGAAATCT 
     4381 ATAGTTCTCT ACGTGGAGTT ACAGAAGCCA TTGAAAAGTT TAGTTTTCGA AGCCAAGAAG 
     4441 ATCTGAATGA GCCAATTAAA CGAGATGGCA AAAAGGAGTG TGATATTGTG TCCCGCGATG 
     4501 GGGGCGCTGC CTCCCCTGCC ACTGAGGGCC GGGGGGGTAG TGAAGTAGAA GGAGGCCGGA 
     4561 CAGCTCTGGA TAACAAGACC TCACTACTCA ACACCCAGCC TCCGCGCGCC TTCCCGGGGC 
     4621 CGCGGGCGCG AGACTACAAC CCGTACCCCT ACTCAGATGC CATCAACACC TACGACAAGA 
     4681 CCGCCCTGAA AGAGGCTGTG TTCGATGACG ACATGGAGCA GCTTCGAGAC GTGCCCATCG 
     4741 ACCATTCTGA CCTGGTGGCT GACCTTCTGA AAGAGCTGTC CAACCACAAT GAGCGAGTGG 
     4801 AGGAACGGAA GGGAGCCCTG CTGGAGCTGC TCAAGATCAC GCGGGAAGAC AGCCTTGGTG 
     4861 TCTGGGAGGA GCACTTCAAG ACCATTCTGC TCCTGCTGCT GGAGACCCTT GGAGACAAAG 
     4921 ACCATTCAAT TCGAGCACTG GCGTTAAGAG TTTTGAGGGA AATTCTGAGA AATCAACCAG 
     4981 CAAGATTTAA AAACTACGCC GAGCTGACGA TTATGAAGAC TCTGGAAGCC CACAAAGACT 
     5041 CCCATAAGGA GGTGGTGAGA GCGGCTGAGG AGGCTGCGTC CACACTGGCC AGTTCCATCC 
     5101 ACCCGGAGCA GTGCATCAAG GTGCTCTGCC CCATCATCCA GACGGCCGAC TACCCCATCA 
     5161 ACCTTGCTGC CATCAAGATG CAGACCAAAG TCGTCGAGAG GATCGCAAAG GAGTCATTGC 
     5221 TGCAGCTCCT TGTCGACATC ATCCCAGGCT TGCTGCAGGG TTATGACAAC ACCGAAAGTA 
     5281 GTGTGCGTAA GGCCAGCGTG TTTTGCTTAG TGGCAATTTA TTCCGTAATC GGAGAAGACC 
     5341 TGAAACCTCA CCTTGCACAG CTCACAGGGA GCAAGATGAA GCTACTAAAC TTATACATAA 
     5401 AGAGGGCCCA GACCACCAAC AGCAACAGCA GCTCCTCCTC CGATGTCTCC ACGCACAGCA 
     5461 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     5521 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     5581 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     5641 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     5701 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     5761 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     5821 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     5881 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     5941 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     6001 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     6061 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     6121 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     6181 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     6241 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     6301 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     6361 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     6421 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     6481 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     6541 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     6601 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     6661 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     6721 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     6781 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     6841 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     6901 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     6961 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     7021 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     7081 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     7141 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     7201 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     7261 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     7321 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     7381 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     7441 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     7501 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     7561 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     7621 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     7681 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     7741 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     7801 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     7861 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     7921 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     7981 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     8041 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     8101 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     8161 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     8221 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     8281 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     8341 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     8401 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     8461 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     8521 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     8581 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     8641 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     8701 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     8761 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     8821 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     8881 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     8941 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     9001 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     9061 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     9121 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     9181 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     9241 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     9301 AACGCGAATT TT
//