LOCUS       RC235310                9270 bp    DNA     circular  SYN 
DEFINITION  KIAA0586 (Myc-DDK tagged) - Homo sapiens KIAA0586 (KIAA0586), transcript variant 3
ACCESSION   NM_001244191
VERSION     NM_001244191.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5427..5518 
                     /label="Myc-DDK"  
     misc_feature    5548..6133 
                     /label="PolyA signal"  
     misc_feature    5605..5624 
                     /label="Restriction Enzyme"  
     misc_feature    6409..7081 
                     /label="ColE1"  
     misc_feature    7511..8305 
                     /label="Kan/Neo"  
     misc_feature    8340..8642 
                     /label="SV40 ori"  
     misc_feature    8728..9205 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5409..5415 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5409 
                     /label="KIAA0586"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTGTCAGAA AGTGATTTTT CTAAAGACGT TGCAGTGCAA GTGTTGCCTT 
     1081 TGGATAAAAT AGAAGAGAAC AACAAGCAAA AAGCAAATGA CATCTTCATT TCTCAGTATA 
     1141 CAATGGGACA GAAAGATGCT CTAAGAACAG TTTTAAAGCA AAAAGCTCAA AGCATGCCTG 
     1201 TTTTTAAGGA AGTAAAGGTA CATCTGTTAG AAGATGCAGG CATAGAGAAG GATGCTGTTA 
     1261 CTCAGGAGAC TAGAATTTCA CCCAGTGGAA TTGATTCAGC TACAACCGTG GCTGCAGCAA 
     1321 CTGCTGCTGC CATTGCAACC GCAGCTCCGT TGATAAAGGT GCAGAGTGAT TTGGAAGCAA 
     1381 AAGTCAATTC TGTTACAGAA TTACTTAGTA AATTACAGGA GACTGATAAA CACCTGCAAC 
     1441 GTGTTACAGA GCAGCAAACA AGCATTCAGA GGAAACAAGA GAAATTACAT TGTCATGATC 
     1501 ACGAAAAGCA AATGAATGTG TTTATGGAGC AGCACATAAG GCATCTTGAA AAGTTACAAC 
     1561 AACAACAAAT AGATATTCAG ACTCATTTTA TTAGTGCTGC ACTCAAGACT AGTAGTTTTC 
     1621 AGCCTGTTAG TATGCCCTCC TCCAGAGCAG TGGAAAAGTA TTCCGTAAAA CCAGAACACC 
     1681 CTAATCTTGG TAGCTGTAAT CCATCTTTAT ATAACACATT TGCTTCCAAA CAAGCACCTT 
     1741 TAAAAGAAGT TGAAGATACG AGTTTTGATA AACAGAAATC TCCTTTGGAG ACACCAGCAC 
     1801 CTCGCAGATT TGCTCCTGTA CCTGTTTCAA GGGATGATGA ACTATCAAAG AGGGAAAATC 
     1861 TTTTGGAAGA AAAAGAAAAT ATGGAAGTGT CGTGTCACAG AGGAAATGTA AGACTATTGG 
     1921 AACAAATTTT GAATAATAAT GATTCTTTGA CAAGAAAAAG TGAATCATCA AACACCACCT 
     1981 CACTAACTAG GTCAAAAATA GGATGGACTC CTGAGAAAAC AAACAGATTT CCTTCCTGTG 
     2041 AAGAGCTAGA AACAACTAAA GTGACTATGC AGAAGTCTGA TGATGTTCTT CATGACCTTG 
     2101 GCCAAAAAGA GAAAGAAACA AATAGCATGG TCCAGCCAAA AGAATCTCTG AGTATGTTGA 
     2161 AGCTTCCAGA TCTTCCACAG AATTCTGTTA AGCTTCAAAC AACCAATACA ACAAGATCTG 
     2221 TATTGAAAGA TGCTGAGAAG ATTTTGAGAG GAGTACAAAA CAATAAAAAA GTACTTGAAG 
     2281 AAAACCTGGA AGCTATTATT CGTGCAAAAG ATGGAGCTGC CATGTATTCG CTTATCAATG 
     2341 CTTTATCTAC CAACAGAGAG ATGTCAGAGA AAATTAGGAT CAGAAAGACA GTGGATGAAT 
     2401 GGATTAAAAC TATTTCTGCA GAAATTCAGG ATGAACTGTC AAGAACAGAT TATGAACAAA 
     2461 AAAGATTTGA TCAGAAGAAT CAGAGAACCA AGAAAGGTCA GAATATGACT AAAGATATTA 
     2521 GAACCAACAC ACAAGATAAA ACTGTCAACA AATCTGTAAT TCCAAGAAAA CATTCTCAAA 
     2581 AGCAAATAGA AGAGCATTTT AGAAATCTAC CTATGAGGGG CATGCCTGCT TCAAGTTTAC 
     2641 AGAAAGAGAG AAAGGAAGGG CTTTTGAAAG CAACCACAGT AATACAAGAT GAAGATTATA 
     2701 TGTTACAAGT CTATGGAAAG CCAGTTTATC AGGGCCATCG AAGCACTCTT AAAAAAGGAC 
     2761 CATATCTCAG ATTTAATTCT CCATCTCCTA AGTCCAGACC ACAGAGACCA AAAGTAATAG 
     2821 AACGAGTTAA AGGCACTAAG GTAAAGTCAA TAAGAACACA GACTGACTTC TATGCAACAA 
     2881 AACCTAAGAA GATGGATTCT AAAATGAAAC ATTCTGTTCC TGTGTTACCT CATGGCGATC 
     2941 AGCAATATTT GTTCAGCCCA AGTAGAGAAA TGCCTACTTT TTCAGGTACA TTGGAAGGTC 
     3001 ATCTGATTCC TATGGCAATT CTTTTAGGAC AAACCCAAAG TAATAGTGAT ACCATGCCAC 
     3061 CTGCTGGAGT GATTGTCAGC AAGCCACACC CTGTAACTGT GACTACTTCT ATTCCTCCAT 
     3121 CATCTCGAAA AGTAGAAACT GGAGTAAAGA AACCTAACAT AGCCATTGTA GAAATGAAGT 
     3181 CAGAAAAAAA GGATCCTCCT CAGCTTACTG TGCAGGTATT ACCCAGTGTA GATATTGACA 
     3241 GCATTTCAAA TAGTAGTGCT GATGTCCTTT CACCTCTGTC TAGCCCCAAA GAAGCATCTC 
     3301 TTCCTCCTGT GCAAACTTGG ATAAAGACTC CAGAAATTAT GAAGGTAGAT GAAGAAGAGG 
     3361 TGAAGTTTCC AGGAACTAAC TTTGATGAAA TAATCGATGT CATACAGGAA GAAGAAAAAT 
     3421 GTGATGAAAT TCCAGACTCT GAACCAATTC TGGAGTTTAA CAGAAGTGTT AAAGCTGATT 
     3481 CTACAAAATA TAATGGTCCT CCATTTCCGC CAGTTGCTTC TACTTTTCAG CCCACTGCTG 
     3541 ATATTCTGGA TAAAGTAATT GAGAGAAAAG AAACACTGGA AAATAGCTTA ATTCAATGGG 
     3601 TAGAGCAAGA AATAATGTCA AGAATTATCT CTGGGCTCTT TCCAGTCCAG CAACAGATTG 
     3661 CACCTAGTAT CAGTGTTTCA GTCAGTGAGA CAAGTGAACC ACTGACTTCT GACATTGTGG 
     3721 AAGGAACAAG CAGTGGCGCC CTCCAGCTTT TTGTTGATGC TGGTGTTCCT GTGAACTCAA 
     3781 ATGTGATTAA ACATTTTGTT AACGAAGCTC TTGCTGAGAC CATTGCTGTC ATGCTGGGTG 
     3841 ACAGAGAAGC AAAGAAGCAA GGTCCTGTTG CTACAGGTGT TTCTGGGGAT GCTTCAACAA 
     3901 ATGAAACATA TTTGCCGGCA AGAGTGTGCA CCCCACTGCC TACCCCACAG CCTACGCCTC 
     3961 CTTGCTCACC TTCATCACCT GCTAAGGAGT GTGTTTTGGT AAAGACTCCA GATTCTTCTC 
     4021 CCTGTGATTC GGATCATGAT ATGGCTTTTC CTGTGAAAGA AATATGTGCT GAAAAAGGAG 
     4081 ATGATATGCC TGCCATCATG CTTGTTAATA CTCCAACAGT TACCCCTACT ACTACACCTC 
     4141 CTCCAGCGGC GGCAGTTTTT ACCCCAACTT TGTCAGATAT TTCCATTGAT AAATTGAAGG 
     4201 TATCAAGCCC AGAGCTTCCC AAGCCATGGG GTGATGGAGA CCTGCCACTG GAAGAAGAGA 
     4261 ACCCTAACTC ACCTCAAGAA GAACTTCATC CAAGAGCTAT TGTAATGTCT GTGGCTAAGG 
     4321 ATGAAGAACC AGAGAGTATG GATTTCCCTG CTCAGCCTCC ACCTCCAGAG CCAGTTCCCT 
     4381 TTATGCCATT TCCTGCCGGC ACCAAGGCCC CTTCCCCCTC ACAGATGCCA GGTTCTGATT 
     4441 CATCAACACT GGAGAGCACA TTGAGTGTTA CTGTCACTGA AACTGAAACT TTAGATAAAC 
     4501 CCATCTCTGA AGGAGAGATT TTATTTAGCT GTGGTCAAAA ATTGGCCCCC AAGATTTTAG 
     4561 AAGATATAGG ACTGTACCTG ACAAACCTTA ATGATAGCTT ATCCAGCACT CTGCATGATG 
     4621 CCGTTGAAAT GGAGGATGAT CCTCCTAGTG AAGGGCAAGT GATTAGGATG TCCCATAAAA 
     4681 AATTTCATGC AGATGCAATT CTTTCTTTTG CTAAACAAAA CCAGGAGTCA GCAGTTTCCC 
     4741 AGCAAGCAGT CTATCATTCA GAGGACTTGG AAAACAGTGT GGGTGAACTT AGTGAAGGAC 
     4801 AAAGACCCCA GCTAACAGCG GCAGCAGAGA ACATCTTAAT GGGACATTCT CTCTATATGC 
     4861 AGCCACCTGT CACTAATACA CAGTCTTTGG ATCAACAATG TGATCCTAAA CCATTATCTC 
     4921 GGCAATTTGA CACAGTTTCA GGTAGTATTT ATGAAGATTC ATGTGCTAGT CATGGTCCAA 
     4981 TGAGTTTGGG AGAATTGGAG TTGGAGCCAA ATTCTAAGCT GGTTCTTCCC ACAACACTTC 
     5041 TGACAGCACA AGAAAATGAT GTTAATTTAC CAGTAGCCGC TGAAGATTTT TCCCAGTACC 
     5101 AACTAAAGCA AAATCAGGAT GTTAAGCAAG TTGAACACAA ACCATCACAA AGTTACCTAC 
     5161 GTGTTAGAAA TAAATCTGAT ATTGCACCTT CACAGCAACA AGTTTCACCA GGTGATATGG 
     5221 ATCGGACACA AATTGAGCTT AATCCGTACC TCACATGTGT ATTTTCAGGT GGGAAAGCAG 
     5281 TGCCACTCTC CGCTTCACAG ATGCCCCCTG CCAAGATGTC AGTGATGCTG CCGTCAGTGA 
     5341 ACCTCGAGGA CTGCTCTCAG TCTCTGAGTC TCAGCACAAT GCAGGAGGAC ATGGAGTCTT 
     5401 CGGGGGCAGA TACCTTCACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     5461 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     5521 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     5581 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     5641 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     5701 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     5761 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     5821 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     5881 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     5941 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     6001 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     6061 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     6121 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     6181 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     6241 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     6301 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     6361 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     6421 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     6481 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     6541 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     6601 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     6661 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     6721 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     6781 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     6841 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     6901 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     6961 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     7021 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     7081 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     7141 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     7201 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     7261 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     7321 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     7381 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     7441 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     7501 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     7561 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     7621 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     7681 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     7741 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     7801 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     7861 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     7921 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     7981 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     8041 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     8101 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     8161 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     8221 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     8281 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     8341 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     8401 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     8461 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     8521 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8581 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     8641 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     8701 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     8761 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     8821 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     8881 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     8941 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     9001 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     9061 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     9121 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     9181 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     9241 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//