LOCUS       RC235305                9231 bp    DNA     circular  SYN 
DEFINITION  TCOF1 (Myc-DDK tagged) - Homo sapiens Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant 7
ACCESSION   NM_001195141
VERSION     NM_001195141.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5388..5479 
                     /label="Myc-DDK"  
     misc_feature    5509..6094 
                     /label="PolyA signal"  
     misc_feature    5566..5585 
                     /label="Restriction Enzyme"  
     misc_feature    6370..7042 
                     /label="ColE1"  
     misc_feature    7472..8266 
                     /label="Kan/Neo"  
     misc_feature    8301..8603 
                     /label="SV40 ori"  
     misc_feature    8689..9166 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5370..5376 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5370 
                     /label="TCOF1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCGAGGCC AGGAAGCGGC GGGAGCTACT TCCCCTGATC TACCACCATC 
     1081 TGCTGCGGGC TGGCTATGTG CGTGCGGCGC GGGAAGTGAA GGAGCAGAGC GGCCAGAAGT 
     1141 GTTTCCTGGC TCAGCCCGTA ACCCTTCTGG ACATCTATAC ACACTGGCAA CAAACCTCAG 
     1201 AGCTTGGTCG GAAGCGGAAG GCAGAGGAAG ATGCGGCACT GCAAGCTAAG AAAACCCGTG 
     1261 TGTCAGACCC CATCAGCACC TCGGAGAGCT CGGAAGAGGA GGAAGAAGCA GAAGCCGAAA 
     1321 CCGCCAAAGC CACCCCAAGA CTAGCATCTA CCAACTCCTC AGTCCTGGGG GCGGACTTGC 
     1381 CATCAAGCAT GAAAGAAAAA GCCAAGGCAG AGACAGAGAA AGCTGGCAAG ACTGGGAATT 
     1441 CCATGCCACA CCCTGCCACT GGGAAGACGG TGGCCAACCT TCTTTCTGGG AAGTCTCCCA 
     1501 GGAAGTCAGC AGAGCCCTCA GCAAATACTA CGTTGGTCTC AGAAACTGAG GAGGAGGGCA 
     1561 GCGTCCCGGC CTTTGGAGCT GCTGCCAAGC CTGGGATGGT GTCAGCGGGC CAGGCCGACA 
     1621 GCTCCAGCGA GGACACCTCC AGCTCCAGTG ATGAGACAGA CGTGGAGGGG AAACCCTCAG 
     1681 TAAAACCAGC CCAGGTCAAA GCCTCATCAG TTTCTACTAA GGAGTCTCCA GCAAGAAAGG 
     1741 CGGCCCCAGC CCCTGGGAAG GTGGGGGATG TGACACCCCA GGTCAAAGGA GGGGCCCTGC 
     1801 CCCCAGCCAA GAGGGCCAAG AAGCCAGAAG AGGAGTCAGA GAGTAGTGAG GAGGGATCTG 
     1861 AAAGTGAGGA GGAGGCCCCT GCAGGGACAC GAAGCCAGGT AAAGGCCTCT GAAAAAATTC 
     1921 TCCAGGTCAG AGCTGCCTCA GCCCCTGCCA AGGGGACCCC TGGGAAAGGG GCTACCCCAG 
     1981 CACCCCCTGG GAAGGCAGGG GCTGTAGCCT CCCAGACCAA GGCAGGGAAG CCAGAGGAGG 
     2041 ACTCAGAGAG CAGCAGCGAG GAGTCATCTG ACAGTGAGGA GGAGACGCCA GCTGCCAAGG 
     2101 CCCTGCTTCA GGCGAAGGCC TCAGGAAAAA CCTCTCAGGT CGGAGCTGCC TCAGCCCCTG 
     2161 CCAAGGAGTC CCCCAGGAAA GGAGCTGCCC CAGCGCCCCC TGGGAAGACA GGGCCTGCAG 
     2221 TTGCCAAGGC CCAGGCGGGG AAGCGGGAGG AGGACTCGCA GAGCAGCAGC GAGGAATCGG 
     2281 ACAGTGAGGA GGAGGCGCCT GCTCAGGCGA AGCCTTCAGG GAAGGCCCCC CAGGTCAGAG 
     2341 CCGCCTCGGC CCCTGCCAAG GAGTCCCCCA GGAAAGGGGC TGCCCCAGCA CCTCCTAGGA 
     2401 AAACAGGGCC TGCAGCCGCC CAGGTCCAGG TGGGGAAGCA GGAGGAGGAC TCAAGAAGCA 
     2461 GCAGCGAGGA GTCAGACAGT GACAGAGAGG CACTGGCAGC CATGAATGCA GCTCAGGTGA 
     2521 AGCCCTTGGG GAAAAGCCCC CAGGTGAAAC CTGCCTCTAC CATGGGCATG GGGCCCTTGG 
     2581 GGAAAGGCGC CGGCCCAGTG CCACCCGGGA AGGTGGGGCC TGCAACCCCC TCAGCCCAGG 
     2641 TGGGGAAGTG GGAGGAGGAC TCAGAGAGCA GTAGTGAGGA GTCATCAGAC AGCAGTGATG 
     2701 GAGAGGTGCC CACAGCTGTG GCCCCGGCTC AGGAAAAGTC CTTGGGGAAC ATCCTCCAGG 
     2761 CCAAACCCAC CTCCAGTCCT GCCAAGGGGC CCCCTCAGAA GGCAGGGCCT GTAGCCGTCC 
     2821 AGGTCAAGGC TGAAAAGCCC ATGGACAACT CGGAGAGCAG CGAGGAGTCA TCGGACAGTG 
     2881 CGGACAGTGA GGAGGCACCA GCAGCCATGA CTGCAGCTCA GGCAAAACCA GCTCTGAAAA 
     2941 TTCCTCAGAC CAAGGCCTGC CCAAAGAAAA CCAATACCAC TGCATCTGCC AAGGTCGCCC 
     3001 CTGTGCGAGT GGGCACCCAA GCCCCCCGGA AAGCAGGAAC TGCGACTTCT CCAGCAGGCT 
     3061 CATCCCCAGC TGTGGCTGGG GGCACCCAGA GACCAGCAGA GGATTCTTCA AGCAGTGAGG 
     3121 AATCAGATAG TGAGGAAGAG AAGACAGGTC TTGCAGTAAC CGTGGGACAG GCAAAGTCTG 
     3181 TGGGGAAAGG CCTCCAGGTG AAAGCAGCCT CAGTGCCTGT CAAGGGGTCC TTGGGGCAAG 
     3241 GGACTGCTCC AGTACTCCCT GGGAAGACGG GGCCTACAGT CACCCAGGTG AAAGCTGAAA 
     3301 AGCAGGAAGA CTCTGAGAGC AGTGAGGAGG AATCAGACAG TGAGGAAGCA GCTGCATCTC 
     3361 CAGCACAGGT GAAAACCTCA GTAAAGAAAA CCCAGGCCAA AGCCAACCCA GCTGCCGCCA 
     3421 GAGCACCTTC AGCAAAAGGG ACAATTTCAG CCCCTGGAAA AGTTGTCACT GCAGCTGCTC 
     3481 AAGCCAAGCA GAGGTCTCCA TCCAAGGTGA AGCCACCAGT GAGAAACCCC CAGAACAGTA 
     3541 CCGTCTTGGC GAGGGGCCCA GCATCTGTGC CATCTGTGGG GAAGGCCGTG GCTACAGCAG 
     3601 CTCAGGCCCA GACAGGGCCA GAGGAGGACT CAGGGAGCAG TGAGGAGGAG TCAGACAGTG 
     3661 AGGAGGAGGC GGAGACGCTG GCTCAGGTGA AGCCTTCAGG GAAGACCCAC CAGATCAGAG 
     3721 CTGCCTTGGC TCCTGCCAAG GAGTCCCCCA GGAAAGGGGC TGCCCCAACA CCTCCTGGGA 
     3781 AGACAGGGCC TTCGGCTGCC CAGGCAGGGA AGCAGGATGA CTCAGGGAGC AGCAGCGAGG 
     3841 AATCAGACAG TGATGGGGAG GCACCGGCAG CTGTGACCTC TGCCCAGGTG ATTAAACCCC 
     3901 CTCTGATTTT TGTCGACCCT AATCGTAGTC CAGCTGGCCC AGCTGCTACA CCCGCACAAG 
     3961 CCCAGGCTGC AAGCACCCCG AGGAAGGCCC GAGCCTCGGA GAGCACAGCC AGGAGCTCCT 
     4021 CCTCCGAGAG CGAGGATGAG GACGTGATCC CCGCTACACA GTGCTTGACT CCTGGCATCA 
     4081 GAACCAATGT GGTGACCATG CCCACTGCCC ACCCAAGAAT AGCCCCCAAA GCCAGCATGG 
     4141 CTGGGGCCAG CAGCAGCAAG GAGTCCAGTC GGATATCAGA TGGCAAGAAA CAGGAGGGAC 
     4201 CAGCCACTCA GGTTGACAGT GCTGTGGGAA CACTCCCTGC AACAAGTCCC CAGAGCACCT 
     4261 CCGTCCAGGC CAAAGGGACC AACAAGCTCA GAAAACCTAA GCTTCCTGAG GTCCAGCAGG 
     4321 CCACCAAAGC CCCTGAGAGC TCAGATGACA GTGAGGACAG CAGCGACAGT TCTTCAGGGA 
     4381 GTGAGGAAGA TGGTGAAGGG CCCCAGGGGG CCAAGTCAGC CCACACGCTG GGTCCCACCC 
     4441 CCTCCAGGAC AGAGACCCTG GTGGAGGAGA CCGCAGCAGA GTCCAGCGAG GATGATGTGG 
     4501 TGGCGCCATC CCAGTCTCTC CTCTCAGGTT ATATGACCCC TGGACTAACC CCAGCCAATT 
     4561 CCCAGGCCTC AAAAGCCACT CCCAAGCTAG ACTCCAGCCC CTCAGTTTCC TCTACTCTGG 
     4621 CCGCCAAAGA TGACCCAGAT GGCAAGCAGG AGGCAAAGCC CCAACAGGCA GCAGGCATGT 
     4681 TGTCCCCTAA AACAGGTGGA AAAGAGGCTG CTTCAGGCAC CACACCTCAG AAGTCCCGGA 
     4741 AGCCCAAGAA AGGGGCTGGG AACCCCCAAG CCTCAACCCT GGCGCTGCAA AGCAACATCA 
     4801 CCCAGTGCCT CCTGGGCCAA CCCTGGCCCC TGAATGAGGC CCAGGTGCAG GCCTCAGTGG 
     4861 TGAAGGTCCT GACTGAGCTG CTGGAACAGG AAAGAAAGAA GGTGGTGGAC ACCACCAAGG 
     4921 AGAGCAGCAG GAAGGGCTGG GAGAGCCGCA AGCGGAAGCT ATCGGGAGAC CAGCCAGCTG 
     4981 CCAGGACCCC CAGGAGCAAG AAGAAGAAGA AGCTGGGGGC CGGGGAAGGT GGGGAGGCCT 
     5041 CTGTTTCCCC AGAAAAGACC TCCACGACTT CCAAGGGGAA AGCAAAGAGA GACAAAGCAA 
     5101 GTGGTGATGT CAAGGAGAAG AAAGGGAAGG GGTCTCTTGG CTCCCAAGGG GCCAAGGACG 
     5161 AGCCAGAAGA GGAGCTTCAG AAGGGGATGG GGACGGTTGA AGGTGGAGAT CAAAGCAACC 
     5221 CAAAGAGCAA GAAGGAGAAG AAGAAATCCG ACAAGAGAAA AAAAGACAAA GAAAAAAAAG 
     5281 AAAAGAAGAA GAAAGCAAAA AAGGCCTCAA CCAAAGATTC TGAGTCACCG TCCCAGAAGA 
     5341 AAAAGAAGAA AAAGAAGAAG ACAGCAGAGC AGACTGTAAC GCGTACGCGG CCGCTCGAGC 
     5401 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     5461 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     5521 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     5581 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     5641 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     5701 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     5761 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     5821 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     5881 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     5941 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     6001 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     6061 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     6121 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     6181 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     6241 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     6301 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     6361 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     6421 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     6481 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     6541 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     6601 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     6661 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     6721 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     6781 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     6841 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     6901 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     6961 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     7021 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     7081 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     7141 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     7201 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     7261 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     7321 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     7381 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     7441 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     7501 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     7561 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     7621 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     7681 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     7741 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     7801 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     7861 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     7921 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     7981 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     8041 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     8101 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     8161 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     8221 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     8281 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     8341 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     8401 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     8461 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     8521 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     8581 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     8641 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     8701 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     8761 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     8821 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     8881 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     8941 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     9001 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     9061 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     9121 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     9181 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
//