LOCUS       RC235301                9183 bp    DNA     circular  SYN 
DEFINITION  AKAP13 (Myc-DDK tagged) - Homo sapiens A kinase (PRKA) anchor protein 13 (AKAP13), transcript variant 4
ACCESSION   NM_001270546
VERSION     NM_001270546.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5340..5431 
                     /label="Myc-DDK"  
     misc_feature    5461..6046 
                     /label="PolyA signal"  
     misc_feature    5518..5537 
                     /label="Restriction Enzyme"  
     misc_feature    6322..6994 
                     /label="ColE1"  
     misc_feature    7424..8218 
                     /label="Kan/Neo"  
     misc_feature    8253..8555 
                     /label="SV40 ori"  
     misc_feature    8641..9118 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5322..5328 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5322 
                     /label="AKAP13"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTATGAACGG CACAAGAGGC GCTACAGCCT CTGTGACATC TCCAAGGTGG 
     1081 ACAGGACTGT GGACGTGGTA TTGCTGAAGA TAAACCGAGA AAACTGGTGT ACAATAGAGC 
     1141 CATGCCCTGA TGCAGCATCT CTTCTGGCTT CCAAGCAGAG CCCAGAATGT GAGAACTTCC 
     1201 TGGATGTTGG ACTGGGCAGA GAGTGTACCT CAAAACAAGG TGTACTTAAA AGAGAATCTG 
     1261 GGAGTGATTC TGACCTCTTT CACTCACCCA GTGATGACAT GGACAGCATC ATCTTCCCAA 
     1321 AGCCAGAGGA AGAGCATTTG GCCTGTGATA TCACCGGATC CAGTTCATCC ACCGATGACA 
     1381 CGGCTTCACT GGACCGACAT TCTTCTCATG GCAGTGATGT GTCTCTCTCC CAGATTTTAA 
     1441 AGCCAAACAG GTCAAGAGAT CGGCAAAGCC TTGATGGATT CTACAGCCAT GGGATGGGAG 
     1501 CTGAGGGTCG AGAAAGTGAG AGTGAGCCTG CTGACCCAGG CGACGTGGAG GAGGAGGAGA 
     1561 TGGACAGTAT CACTGAAGTG CCTGCAAACT GCTCTGTCCT AAGGAGCTCC ATGCGCTCTC 
     1621 TTTCTCCCTT CCGGAGGCAC AGCTGGGGGC CTGGGAAAAA TGCAGCCAGC GATGCAGAAA 
     1681 TGAACCACCG GAGTTTCAGT CTAGAAGGCT TGACAGGAGG AGCTGGTGTC GGAAACAAGC 
     1741 CATCCTCATC TCTAGAAGTA AGCTCTGCAA ATGCCGAAGA GCTCAGACAC CCATTCAGTG 
     1801 GTGAGGAACG GGTTGACTCT TTGGTGTCAC TTTCAGAAGA GGATCTGGAG TCAGACCAGA 
     1861 GAGAACATAG GATGTTTGAT CAGCAGATAT GTCACAGATC TAAGCAGCAG GGATTTAATT 
     1921 ACTGTACATC AGCCATTTCC TCTCCATTGA CAAAATCCAT CTCATTAATG ACAATCAGCC 
     1981 ATCCTGGATT GGACAATTCA CGGCCCTTCC ACAGTACCTT CCACAATACC AGTGCTAATC 
     2041 TGACTGAGAG TATAACAGAA GAGAACTATA ATTTCCTGCC ACATAGCCCC TCCAAGAAAG 
     2101 ATTCTGAATG GAAGAGTGGA ACAAAAGTCA GTCGTACATT CAGCTACATC AAGAATAAAA 
     2161 TGTCTAGCAG CAAGAAGAGC AAAGAAAAGG AAAAAGAAAA AGATAAGATT AAGGAGAAGG 
     2221 AGAAAGATTC TAAAGACAAG GAGAAAGATA AGAAGACTGT CAACGGGCAC ACTTTCAGTT 
     2281 CCATTCCTGT TGTGGGTCCC ATCAGCTGTA GCCAGTGTAT GAAGCCCTTC ACCAACAAAG 
     2341 ATGCCTATAC TTGTGCAAAT TGCAGTGCTT TTGTCCACAA AGGCTGCCGA GAAAGTCTAG 
     2401 CCTCCTGTGC AAAGGTCAAA ATGAAGCCCA AAGGGAGCCT TCAGGCACAT GACACATCAT 
     2461 CACTGCCCAC GGTCATTATG AGAAACAAGC CCTCACAGCC CAAGGAGCGT CCTCGGTCCG 
     2521 CAGTCCTCCT GGTGGATGAA ACCGCTACCA CCCCAATATT TGCCAATAGA CGATCCCAGC 
     2581 AGAGTGTCTC GCTCTCCAAA AGTGTCTCCA TACAGAACAT TACTGGAGTT GGCAATGATG 
     2641 AGAACATGTC AAACACCTGG AAATTCCTGT CTCATTCAAC AGACTCACTA AATAAAATCA 
     2701 GCAAGGTCAA TGAGTCAACA GAATCACTTA CTGATGAGGG AGTAGGTACA GACATGAATG 
     2761 AAGGACAACT ACTGGGAGAC TTTGAGATTG AGTCCAAACA GCTGGAAGCA GAGTCTTGGA 
     2821 GTCGGATAAT AGACAGCAAG TTTCTAAAAC AGCAAAAGAA AGATGTGGTC AAACGGCAAG 
     2881 AAGTAATATA TGAGTTGATG CAGACAGAGT TTCATCATGT CCGCACTCTC AAGATCATGA 
     2941 GTGGTGTGTA CAGCCAGGGG ATGATGGCGG ATCTGCTTTT TGAGCAGCAG ATGGTAGAAA 
     3001 AGCTGTTCCC CTGTTTGGAT GAGCTGATCA GTATCCATAG CCAATTCTTC CAGAGGATTC 
     3061 TGGAGCGGAA GAAGGAGTCT CTGGTGGATA AAAGTGAAAA GAACTTTCTC ATCAAGAGGA 
     3121 TAGGGGATGT GCTTGTAAAT CAGTTTTCAG GTGAGAATGC AGAACGTTTA AAGAAGACAT 
     3181 ATGGCAAGTT TTGTGGGCAA CATAACCAGT CTGTAAACTA CTTCAAAGAC CTTTATGCCA 
     3241 AGGATAAGCG TTTTCAAGCC TTTGTAAAGA AGAAGATGAG CAGTTCAGTT GTTAGAAGGC 
     3301 TTGGAATTCC AGAGTGCATA TTGCTTGTAA CTCAGCGGAT TACCAAGTAC CCAGTTTTAT 
     3361 TCCAAAGAAT ATTGCAGTGT ACCAAAGACA ATGAAGTGGA GCAGGAAGAT CTAGCACAGT 
     3421 CCTTGAGCCT GGTGAAGGAT GTGATTGGAG CTGTAGACAG CAAAGTGGCA AGTTATGAAA 
     3481 AGAAAGTGCG TCTCAATGAG ATTTATACAA AGACAGATAG CAAGTCAATC ATGAGGATGA 
     3541 AGAGTGGTCA GATGTTTGCC AAGGAAGATT TGAAACGGAA GAAGCTTGTA CGTGATGGGA 
     3601 GTGTGTTTCT GAAGAATGCA GCAGGAAGGT TGAAAGAGGT TCAAGCAGTT CTTCTCACTG 
     3661 ACATTTTAGT TTTCCTTCAA GAAAAAGACC AGAAGTACAT CTTTGCATCA TTGGACCAGA 
     3721 AGTCAACAGT GATCTCTTTA AAGAAGCTGA TTGTGAGAGA AGTGGCACAT GAGGAGAAAG 
     3781 GTTTATTCCT GATCAGCATG GGGATGACAG ATCCAGAGAT GGTAGAAGTC CATGCCAGCT 
     3841 CCAAAGAGGA ACGAAACAGC TGGATTCAGA TCATTCAGGA CACAATCAAC ACCCTGAACA 
     3901 GAGATGAAGA TGAAGGAATT CCTAGTGAGA ATGAGGAAGA AAAGAAAATG TTGGACACCA 
     3961 GAGCCCGAGA ATTAAAAGAA CAACTTCACC AGAAGGACCA AAAAATCCTA CTCTTGTTGG 
     4021 AAGAGAAGGA GATGATTTTC CGGGACATGG CTGAGTGCAG CACCCCTCTC CCAGAGGATT 
     4081 GCTCCCCAAC ACATAGCCCT AGAGTTCTCT TCCGCTCCAA CACAGAAGAG GCTCTCAAAG 
     4141 GAGGACCTTT AATGAAAAGT GCAATAAATG AGGTGGAGAT CCTTCAGGGT TTGGTGAGTG 
     4201 GAAATCTGGG AGGCACACTT GGGCCGACTG TCAGCAGCCC CATTGAGCAA GATGTGGTCG 
     4261 GTCCCGTTTC CCTGCCCCGG AGAGCAGAGA CCTTTGGAGG ATTTGACAGC CATCAGATGA 
     4321 ATGCTTCAAA AGGAGGCGAG AAGGAAGAGG GAGATGATGG CCAAGATCTT AGGAGAACGG 
     4381 AATCAGATAG TGGCCTAAAA AAGGGTGGAA ATGCTAACCT GGTATTTATG CTTAAAAGAA 
     4441 ACAGTGAGCA GGTTGTCCAG AGCGTTGTTC ATCTCTACGA GCTCCTCAGC GCTCTGCAGG 
     4501 GTGTGGTGCT GCAGCAGGAC AGCTACATTG AGGACCAGAA ACTGGTGCTG AGCGAGAGGG 
     4561 CGCTCACTCG CAGCTTGTCC CGCCCGAGCT CCCTCATTGA GCAGGAGAAG CAGCGCAGCC 
     4621 TGGAGAAGCA GCGCCAGGAC CTGGCCAACC TGCAGAAGCA GCAGGCCCAG TACCTCGAGG 
     4681 AGAAGCGCAG GCGCGAGCGT GAGTGGGAAG CTCGTGAGAG GGAGCTGCGG GAGCGGGAGG 
     4741 CCCTCCTGGC CCAGCGCGAG GAGGAGGTGC AGCAGGGGCA GCAGGACCTG GAAAAGGAGC 
     4801 GGGAGGAGCT CCAGCAGAAG AAGGGCACAT ACCAGTATGA CCTGGAGCGA CTGCGTGCTG 
     4861 CCCAGAAACA GCTTGAGAGG GAACAGGAGC AGCTGCGCCG GGAGGCAGAG CGGCTCAGCC 
     4921 AGCGGCAGAC AGAACGGGAC CTGTGTCAGG TTTCCCATCC ACATACCAAG CTGATGAGGA 
     4981 TCCCATCGTT CTTCCCCAGT CCTGAGGAGC CCCCCTCGCC ATCTGCACCT TCCATAGCCA 
     5041 AATCAGGGTC ATTGGACTCA GAACTTTCAG TGTCCCCAAA AAGGAACAGC ATCTCTCGGA 
     5101 CACACAAAGA TAAGGGGCCT TTTCACATAC TGAGTTCAAC CAGCCAGACA AACAAAGGAC 
     5161 CAGAAGGGCA GAGCCAGGCC CCTGCGTCCA CCTCTGCCTC TACCCGCCTG TTTGGGTTAA 
     5221 CAAAGCCAAA GGAAAAGAAG GAGAAAAAAA AGAAGAACAA AACCAGCCGC TCTCAGCCCG 
     5281 GTGATGGTCC CGCGTCAGAA GTATCAGCAG AGGGTGAAGA GATCTTCTGC ACGCGTACGC 
     5341 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     5401 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     5461 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     5521 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     5581 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     5641 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     5701 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     5761 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     5821 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     5881 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     5941 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     6001 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     6061 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     6121 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     6181 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     6241 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     6301 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     6361 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     6421 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     6481 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     6541 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     6601 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     6661 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     6721 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     6781 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     6841 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     6901 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     6961 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     7021 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     7081 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     7141 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     7201 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     7261 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     7321 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     7381 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     7441 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     7501 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     7561 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     7621 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     7681 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     7741 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     7801 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     7861 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     7921 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     7981 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     8041 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     8101 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     8161 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     8221 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     8281 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     8341 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     8401 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     8461 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     8521 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     8581 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     8641 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     8701 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     8761 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     8821 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     8881 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     8941 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     9001 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     9061 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     9121 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     9181 TTT
//