LOCUS       RC235299                9147 bp    DNA     circular  SYN 
DEFINITION  USP19 (Myc-DDK tagged) - Homo sapiens ubiquitin specific peptidase 19 (USP19), transcript variant 1
ACCESSION   NM_001199160
VERSION     NM_001199160.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5302..5393 
                     /label="Myc-DDK"  
     misc_feature    5423..6008 
                     /label="PolyA signal"  
     misc_feature    5480..5499 
                     /label="Restriction Enzyme"  
     misc_feature    6284..6956 
                     /label="ColE1"  
     misc_feature    7386..8180 
                     /label="Kan/Neo"  
     misc_feature    8215..8517 
                     /label="SV40 ori"  
     misc_feature    8603..9080 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5277..5284 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5277 
                     /label="USP19"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCTGGCGGG GCCAGTGCCA CAGGCCCAAG GAGAGGGCCC CCAGGACTGG 
     1081 AGGACACCAC TAGTAAGAAG AAGCAGAAGG ATCGAGCAAA CCAGGAGAGC AAGGATGGAG 
     1141 ATCCTAGGAA AGAGACAGGG TCTCGATATG TTGCCCAGGC TGGTCTTGAA CCTCTGGCCT 
     1201 CAGGTGATCC TTCTGCCTCA GCCTCCCATG CAGCTGGGAT CACAGGCTCA CGCCACCGTA 
     1261 CCCGGCTGTT CTTTCCTTCA TCGTCAGGGT CAGCATCCAC TCCTCAAGAG GAGCAGACCA 
     1321 AAGAGGGAGC TTGTGAAGAC CCTCATGATC TCTTGGCTAC TCCCACTCCA GAGTTGTTGC 
     1381 TCGATTGGAG GCAGAGTGCA GAAGAGGTGA TTGTCAAGCT TCGTGTGGGA GTAGGTCCCC 
     1441 TGCAGCTGGA GGATGTAGAT GCTGCTTTCA CAGATACAGA CTGTGTGGTG CGGTTTGCAG 
     1501 GTGGTCAGCA GTGGGGTGGT GTCTTCTATG CTGAGATAAA AAGCTCTTGT GCTAAAGTGC 
     1561 AAACCCGCAA GGGCAGTCTC CTGCACCTGA CACTGCCCAA AAAGGTGCCT ATGCTCACGT 
     1621 GGCCCTCCCT CCTGAAGAAA CCTCTAGGGA CCCAGGAGCT GGTGCCGGGG CTGCGGTGCC 
     1681 AGGAGAATGG GCAGGAACTG TCTCCCATTG CCCTGGAGCC AGGCCCTGAG CCCCACCGGG 
     1741 CTAAGCAGGA GGCCCGGAAC CAGAAGCGGG CCCAGGGCCG TGGTGAGGTA GGCGCAGGGG 
     1801 CTGGCCCCGG GGCCCAGGCA GGGCCCAGCG CCAAGAGGGC TGTGCATCTC TGCAGAGGGC 
     1861 CAGAGGGGGA CGGGTCCAGG GATGACCCTG GACCCCGGGG TGATGCCCCA CCCTTCGTGG 
     1921 CTGACCCAGC CACCCAGGTT GAGGCTGATG AACAGCTTTG CATACCACCG CTGAACTCCC 
     1981 AAACCTGCCT CCTGGGCTCA GAGGAGAATT TAGCCCCTTT GGCAGGAGAG AAAGCAGTGC 
     2041 CTCCCGGGAA TGACCCAGTC TCTCCAGCCA TGGTCCGGAG CAGAAACCCT GGGAAAGATG 
     2101 ACTGTGCCAA GGAGGAGATG GCAGTGGCAG CAGATGCTGC AACCTTGGTG GATGAGCCCG 
     2161 AGTCGATGGT GAACCTGGCG TTTGTCAAGA ATGACTCGTA TGAGAAGGGC CCGGATTCAG 
     2221 TGGTGGTGCA CGTGTACGTG AAGGAGATCT GCAGGGACAC CTCAAGAGTA CTTTTCCGTG 
     2281 AGCAGGACTT CACGCTCATC TTCCAGACCA GGGATGGAAA CTTCCTGAGG CTGCACCCGG 
     2341 GCTGTGGGCC CCACACCACC TTCCGTTGGC AGGTGAAGCT CAGGAATCTG ATTGAGCCAG 
     2401 AGCAGTGCAC CTTCTGTTTC ACGGCTTCTC GCATCGACAT CTGCCTTCGT AAGAGGCAGA 
     2461 GTCAGCGCTG GGGGGGCCTG GAGGCCCCGG CTGCACGAGT GGGTGGTGCA AAGGTTGCCG 
     2521 TGCCGACAGG TCCAACCCCT CTGGATTCAA CCCCACCAGG AGGTGCTCCC CACCCCCTGA 
     2581 CAGGCCAGGA GGAGGCCCGG GCTGTGGAGA AGGATAAATC CAAGGCACGA TCTGAGGACA 
     2641 CAGGGCTAGA CAGTGTGGCA ACCCGCACAC CCATGGAGCA TGTAACCCCA AAGCCAGAGA 
     2701 CACACCTGGC CTCGCCCAAG CCTACATGCA TGGTGCCTCC CATGCCCCAC AGCCCAGTTA 
     2761 GTGGAGACAG CGTGGAGGAG GAGGAAGAGG AAGAGAAGAA GGTGTGTCTG CCAGGCTTCA 
     2821 CTGGCCTTGT CAATTTAGGC AACACCTGCT TCATGAACAG CGTCATTCAG TCTCTGTCCA 
     2881 ACACTCGGGA ACTCCGGGAC TTCTTCCATG ACCGCTCCTT TGAGGCTGAG ATCAACTACA 
     2941 ACAACCCACT AGGGACTGGT GGGCGTCTGG CCATTGGCTT TGCCGTGCTG CTTCGGGCGC 
     3001 TGTGGAAGGG CACCCACCAT GCCTTCCAGC CTTCCAAGTT GAAGGCCATT GTGGCGAGTA 
     3061 AGGCCAGCCA GTTCACAGGC TATGCACAGC ATGATGCCCA GGAGTTCATG GCTTTCCTGC 
     3121 TGGATGGGCT GCACGAGGAC CTGAATCGCA TTCAGAACAA GCCCTACACA GAGACCGTGG 
     3181 ATTCAGATGG GCGGCCCGAT GAGGTGGTAG CTGAGGAAGC ATGGCAGCGG CACAAGATGA 
     3241 GGAATGACTC TTTCATCGTG GACCTATTTC AGGGGCAGTA CAAGTCGAAG CTGGTGTGCC 
     3301 CTGTGTGTGC CAAGGTCTCC ATCACTTTTG ACCCGTTTCT TTATCTGCCG GTGCCCTTGC 
     3361 CACAAAAGCA AAAGGTTCTC CCTGTCTTTT ATTTTGCCCG AGAGCCCCAC AGCAAGCCCA 
     3421 TCAAGTTCCT GGTGAGCGTC AGCAAGGAGA ACTCCACTGC GAGCGAAGTA TTGGACTCCC 
     3481 TCTCTCAGAG TGTTCATGTG AAGCCTGAGA ACCTGCGTTT GGCGGAGGTA ATTAAGAATC 
     3541 GTTTTCATCG TGTGTTCCTA CCCTCCCACT CACTGGACAC TGTGTCCCCA TCTGATACGC 
     3601 TCCTCTGCTT TGAGCTGCTA TCCTCAGAGT TGGCTAAGGA GCGGGTAGTG GTGCTAGAGG 
     3661 TGCAACAGCG CCCCCAGGTG CCCAGCGTCC CCATCTCCAA GTGTGCAGCC TGCCAGCGGA 
     3721 AGCAACAGTC GGAGGATGAA AAGCTGAAGC GCTGTACCCG GTGCTACCGT GTGGGCTACT 
     3781 GCAACCAGCT CTGCCAGAAA ACCCACTGGC CTGACCACAA GGGCCTCTGC CGACCTGAGA 
     3841 ACATTGGCTA CCCCTTCCTG GTCAGTGTAC CTGCCTCACG CCTCACTTAT GCCCGCCTCG 
     3901 CTCAGTTGCT AGAGGGCTAT GCCCGGTACT CTGTGAGTGT ATTCCAGCCA CCCTTTCAGC 
     3961 CAGGCCGCAT GGCCTTGGAG TCTCAGAGCC CTGGCTGCAC CACACTGCTC TCCACAGGTT 
     4021 CCCTGGAGGC TGGGGACAGC GAGAGAGACC CCATTCAGCC ACCTGAGCTC CAGCTGGTGA 
     4081 CCCCTATGGC TGAGGGGGAC ACAGGGCTTC CCCGGGTGTG GGCAGCCCCT GACCGGGGTC 
     4141 CTGTGCCCAG CACCAGTGGA ATTTCTTCTG AGATGCTGGC CAGTGGGCCC ATTGAGGTTG 
     4201 GCTCCTTGCC AGCTGGCGAG AGGGTGTCCC GACCCGAAGC TGCTGTGCCT GGGTACCAGC 
     4261 ATCCAAGTGA AGCTATGAAT GCCCACACAC CCCAGTTCTT CATCTATAAA ATTGATTCAT 
     4321 CCAACCGAGA GCAGCGGCTA GAGGACAAAG GAGACACCCC ACTGGAGCTG GGTGACGACT 
     4381 GTAGCCTGGC TCTCGTCTGG CGGAACAATG AGCGCTTGCA GGAGTTTGTG TTGGTAGCCT 
     4441 CCAAGGAGCT GGAATGTGCT GAGGATCCAG GCTCTGCCGG TGAGGCTGCC CGGGCCGGCC 
     4501 ACTTCACCCT GGACCAGTGC CTCAACCTCT TCACACGGCC TGAGGTGCTG GCACCCGAGG 
     4561 AGGCCTGGTA CTGCCCACAG TGCAAACAGC ACCGTGAGGC CTCCAAGCAG CTGTTGCTAT 
     4621 GGCGCCTGCC AAATGTTCTC ATCGTGCAGC TCAAGCGCTT CTCCTTTCGT AGTTTTATCT 
     4681 GGCGTGACAA GATCAATGAC TTGGTGGAGT TCCCTGTTAG GAACCTGGAC CTGAGCAAGT 
     4741 TCTGCATTGG TCAGAAAGAG GAGCAGCTGC CCAGCTACGA TCTATATGCT GTCATCAACC 
     4801 ACTATGGAGG CATGATTGGT GGCCACTACA CTGCCTGTGC ACGCCTGCCC AATGATCGTA 
     4861 GCAGTCAGCG CAGTGACGTG GGCTGGCGCT TGTTTGATGA CAGCACAGTG ACAACGGTAG 
     4921 ACGAGAGCCA GGTTGTGACG CGTTATGCCT ATGTACTCTT CTACCGCCGG CGGAACTCTC 
     4981 CTGTGGAGAG GCCCCCCAGG GCAGGTCACT CTGAGCACCA CCCAGACCTA GGCCCTGCAG 
     5041 CTGAGGCTGC TGCCAGCCAG GCTTCCCGGA TTTGGCAGGA GCTGGAGGCT GAGGAGGAGC 
     5101 CGGTGCCTGA GGGGTCTGGG CCCCTGGGTC CCTGGGGGCC CCAAGACTGG GTGGGCCCCC 
     5161 TACCACGTGG CCCTACCACA CCAGATGAGG GCTGCCTCCG GTACTTTGTC CTGGGCACCG 
     5221 TGGCGGCTTT GGTGGCCCTC GTGCTCAACG TGTTCTATCC TCTGGTATCC CAGAGTCGCT 
     5281 GGAGAAGCGG ACCGACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     5341 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     5401 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     5461 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     5521 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     5581 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     5641 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     5701 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     5761 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     5821 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     5881 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     5941 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     6001 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     6061 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     6121 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     6181 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     6241 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     6301 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     6361 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     6421 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     6481 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     6541 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     6601 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     6661 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     6721 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     6781 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     6841 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     6901 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     6961 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     7021 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     7081 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     7141 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     7201 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     7261 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     7321 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     7381 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     7441 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     7501 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     7561 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     7621 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     7681 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     7741 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     7801 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     7861 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     7921 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     7981 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     8041 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     8101 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     8161 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     8221 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     8281 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     8341 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     8401 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     8461 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     8521 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     8581 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     8641 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     8701 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     8761 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     8821 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     8881 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     8941 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     9001 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     9061 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     9121 ATTTAACAAA AATTTAACGC GAATTTT
//