LOCUS       RC235295                9057 bp    DNA     circular  SYN 
DEFINITION  ARHGEF28 (Myc-DDK tagged) - Homo sapiens Rho guanine nucleotide exchange factor (GEF) 28 (ARHGEF28), transcript variant 3
ACCESSION   NM_001244364
VERSION     NM_001244364.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5214..5305 
                     /label="Myc-DDK"  
     misc_feature    5335..5920 
                     /label="PolyA signal"  
     misc_feature    5392..5411 
                     /label="Restriction Enzyme"  
     misc_feature    6196..6868 
                     /label="ColE1"  
     misc_feature    7298..8092 
                     /label="Kan/Neo"  
     misc_feature    8127..8429 
                     /label="SV40 ori"  
     misc_feature    8515..8992 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5196..5202 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5196 
                     /label="ARHGEF28"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATTCTGAC TCCGACTCAC CTTTTAACTA CTCGTGGCCT TCCTTTCCCA 
     1081 AAATGAAGAT TCGAAGAAGG ACGTCCAAAC AAGATCGCTC CTTCGATATC CTAAAAAAAT 
     1141 CCAAGCCGCC CTCGACATTG CTTGCTGCAG GCCGGCTTTC AGACATGCTG AATGGAGGTG 
     1201 ATGAAGTCTA CGCTAACTGT ATGGTGATTG ATCAGGTTGG TGATTTGGAT ATCAGCTATA 
     1261 TTAATATAGA GGGAATCACT GCCACTACCA GCCCTGAATC CAGAGGTTGC ACTCTGTGGC 
     1321 CTCAGAGCAG CAAACACACC CTTCCTACAG AAACCAGTCC CAGTGTGTAC CCACTTAGTG 
     1381 AAAATGTCGA AGGGACAGCA CACACTGAAG CCCAGCAGTC CTTCATGTCA CCATCAAGTT 
     1441 CGTGTGCTTC CAACTTGAAT CTTTCTTTTG GTTGGCATGG ATTTGAAAAG GAACAAAGTC 
     1501 ATCTAAAGAA AAGAAGTTCT AGCCTTGATG CCTTGGACGC CGACAGTGAA GGGGAAGGGC 
     1561 ATTCTGAGCC ATCCCACATC TGTTACACTC CAGGGTCTCA GAGCTCCTCA AGAACTGGGA 
     1621 TTCCTAGTGG GGATGAATTG GACTCTTTTG AGACTAACAC TGAACCGGAT TTTAATATCT 
     1681 CCAGGGCTGA ATCCCTTCCT CTATCAAGTA ATCTACAGTC GAAGGAATCA CTGCTTTCTG 
     1741 GAGTTCGCTC ACGTTCTTAT TCTTGCTCAT CACCCAAAAT TTCTTTAGGA AAAACTCGTT 
     1801 TGGTGCGTGA ATTAACAGTA TGCAGTTCAA GTGAAGAGCA AAGAGCTTAC AGCTTATCGG 
     1861 AGCCACCAAG AGAAAACAGA ATTCAGGAAG AAGAATGGGA TAAATACATC ATACCTGCCA 
     1921 AATCAGAGTC TGAAAAATAT AAAGTGAGTC GAACTTTCAG TTTCCTCATG AATAGGATGA 
     1981 CTAGCCCTCG GAATAAATCA AAGACAAAAA GCAAGGATGC CAAAGATAAA GAGAAGCTGA 
     2041 ATCGACATCA GTTTGCCCCA GGAACATTCT CTGGGGTTCT GCAGTGTTTG GTTTGTGATA 
     2101 AAACACTCCT GGGGAAAGAG TCACTGCAGT GTTCTAACTG TAATGCAAAT GTGCACAAAG 
     2161 GTTGTAAAGA TGCTGCGCCT GCATGCACCA AGAAATTCCA AGAGAAATAT AACAAGAACA 
     2221 AACCACAGAC CATCCTTGGA AATTCTTCAT TTAGAGACAT CCCACAGCCT GGTCTCTCCT 
     2281 TGCACCCTTC TTCCTCCGTG CCTGTTGGAT TGCCGACTGG AAGGAGGGAG ACTGTGGGAC 
     2341 AGGTCCATCC ATTGTCCAGA AGTGTTCCAG GCACCACCTT GGAAAGCTTC AGGAGGTCAG 
     2401 CCACATCCTT GGAGTCTGAG AGTGACCATA ACAGCTGCAG AAGCAGGTCT CATTCTGATG 
     2461 AGCTGCTACA GTCCATGGGC TCTTCTCCCT CTACAGAGTC TTTCATAATG GAAGATGTTG 
     2521 TGGATTCTTC TCTGTGGAGT GACCTCAGCA GTGATGCCCA GGAGTTTGAA GCAGAATCTT 
     2581 GGAGTCTTGT GGTGGATCCC TCATTTTGTA ATAGGCAGGA GAAGGATGTC ATCAAAAGAC 
     2641 AGGATGTCAT TTTTGAGCTA ATGCAAACAG AGATGCATCA CATCCAGACC CTGTTCATCA 
     2701 TGTCTGAGAT CTTCAGGAAA GGCATGAAAG AGGAGCTGCA GCTGGACCAC AGCACCGTGG 
     2761 ATAAAATTTT CCCCTGTTTA GATGAGTTGC TTGAAATCCA CAGGCATTTC TTCTACAGTA 
     2821 TGAAGGAACG AAGGCAGGAA TCCTGTGCTG GCAGCGACAG GAATTTTGTG ATCGACCGAA 
     2881 TTGGAGATAT TTTGGTACAA CAGTTTTCAG AAGAAAATGC AAGTAAAATG AAGAAAATAT 
     2941 ATGGAGAATT CTGTTGCCAT CATAAAGAAG CTGTTAACCT CTTTAAAGAA CTCCAGCAGA 
     3001 ATAAAAAGTT TCAGAATTTT ATTAAGCTCC GAAATAGTAA TCTTTTGGCT CGACGCCGAG 
     3061 GAATTCCAGA ATGCATTCTG TTGGTCACTC AGCGTATTAC AAAATACCCT GTCTTGGTGG 
     3121 AAAGGATATT GCAGTACACA AAGGAAAGAA CTGAGGAACA TAAAGACTTA CGCAAAGCGC 
     3181 TTTGCTTAAT TAAAGACATG ATTGCAACAG TGGATTTAAA AGTCAATGAA TATGAGAAAA 
     3241 ACCAAAAATG GCTTGAGATC CTAAATAAGA TTGAAAACAA AACATACACG AAGCTCAAAA 
     3301 ATGGACATGT GTTTAGGAAG CAGGCACTGA TGAGTGAAGA AAGGACTCTG TTATATGATG 
     3361 GCCTTGTTTA CTGGAAAACT GCTACAGGTC GTTTCAAAGA TATCCTAGCT CTACTTCTAA 
     3421 CTGATGTGCT GCTCTTTTTA CAAGAAAAAG ACCAGAAATA CATCTTTGCA GCCGTTGATC 
     3481 AGAAGCCATC AGTTATTTCC CTTCAAAAGC TTATTGCTAG AGAAGTTGCT AATGAGGAGA 
     3541 GAGGAATGTT TCTGATCAGT GCTTCATCTG CTGGTCCTGA GATGTATGAA ATTCACACCA 
     3601 ATTCCAAGGA GGAACGCAAT AACTGGATGA GACGGATCCA GCAGGCTGTA GAAAGTTGTC 
     3661 CTGAAGAAAA AGGGGGAAGG ACAAGTGAAT CTGATGAAGA CAAGAGGAAA GCTGAAGCCA 
     3721 GAGTGGCCAA AATTCAGCAA TGTCAAGAAA TACTCACTAA CCAAGACCAA CAAATTTGTG 
     3781 CGTATTTGGA GGAGAAGCTG CATATCTATG CTGAACTTGG AGAACTGAGC GGATTTGAGG 
     3841 ACGTCCATCT AGAGCCCCAC CTCCTTATTA AACCTGACCC AGGCGAGCCT CCCCAGGCAG 
     3901 CCTCATTACT GGCAGCAGCA CTGAAAGAAG CTGAGAGCCT ACAAGTTGCA GTGAAGGCCT 
     3961 CACAGATGGG CGCCGTGAGT CAATCATGTG AGGACAGTTG TGGAGACTCT GTCTTGGCGG 
     4021 ACACACTCAG TTCTCATGAT GTACCAGGAT CACCGACTGC CTCATTAGTC ACAGGAGGGA 
     4081 GAGAAGGAAG AGGCTGTTCG GATGTGGATC CCGGGATCCA GGGTGTGGTA ACCGACTTGG 
     4141 CCGTCTCTGA TGCAGGGGAG AAGGTGGAAT GTAGAAATTT TCCAGGTTCT TCACAATCAG 
     4201 AGATTATACA AGCCATACAG AATTTAACCC GTCTCTTATA CAGCCTTCAG GCCGCCTTGA 
     4261 CCATTCAGGA CAGCCACATT GAGATCCACA GGCTGGTTCT CCAGCAGCAG GAGGGCCTGT 
     4321 CTCTCGGCCA CTCTATCCTC CGAGGCGGCC CCTTGCAGGA CCAGAAGTCT CGCGACGCGG 
     4381 ACAGGCAGCA TGAGGAGCTG GCCAATGTGC ACCAGCTTCA GCACCAGCTC CAGCAGGAGC 
     4441 AGCGGCGCTG GCTGCGCAGG TGTGAGCAGC AGCAGCGGGC GCAGGCGACC AGGGAGAGCT 
     4501 GGCTGCAGGA GCGGGAGCGG GAGTGCCAGT CGCAGGAGGA GCTGCTGCTG CGGAGCCGGG 
     4561 GCGAGCTGGA CCTCCAGCTC CAGGAGTACC AGCACAGCCT GGAGCGGCTG AGGGAGGGCC 
     4621 AGCGCCTGGT GGAGAGGGAG CAGGCGAGGA TGCGGGCCCA GCAGAGCCTG CTGGGCCACT 
     4681 GGAAGCACGG CCGGCAGAGG AGCCTGCCCG CGGTGCTCCT TCCGGGTGGC CCCGAGGTAA 
     4741 TGGAACTTAA TCGATCTGAG AGTTTATGTC ATGAAAACTC ATTCTTCATC AATGAAGCTT 
     4801 TAGTACAAAT GTCATTTAAC ACTTTCAACA AACTGAATCC ATCAGTTATC CATCAGGATG 
     4861 CCACTTACCC TACAACTCAA TCTCATTCTG ACTTGGTGAG GACTAGTGAA CATCAAGTAG 
     4921 ACCTCAAGGT GGACCCTTCT CAGCCTTCGA ATGTCAGTCA CAAACTGTGG ACAGCCGCTG 
     4981 GTTCCGGCCA TCAGATACTT CCTTTCCATG AAAGCAGCAA GGATTCTTGT AAAAATGATT 
     5041 TGGACACCTC CCACACTGAG TCCCCAACCC CCCATGACTC AAATTCACAC CGCCCTCAAC 
     5101 TGCAGGCGTT TATAACAGAA GCAAAGCTAA ATCTACCGAC AAGGACAATG ACCAGACAAG 
     5161 ATGGGGAAAC TGGAGATGGA GCCAAAGAAA ATATTGTTTA CCTCACGCGT ACGCGGCCGC 
     5221 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     5281 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     5341 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     5401 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     5461 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     5521 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     5581 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     5641 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     5701 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     5761 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     5821 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     5881 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5941 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     6001 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     6061 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     6121 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     6181 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     6241 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     6301 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     6361 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     6421 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     6481 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     6541 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     6601 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     6661 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     6721 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     6781 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     6841 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     6901 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     6961 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     7021 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     7081 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     7141 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     7201 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     7261 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     7321 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     7381 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     7441 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     7501 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     7561 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     7621 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     7681 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     7741 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     7801 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     7861 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     7921 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     7981 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     8041 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     8101 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     8161 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     8221 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     8281 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8341 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     8401 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     8461 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     8521 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     8581 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     8641 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     8701 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     8761 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     8821 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     8881 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     8941 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     9001 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//