LOCUS       RC235291                9042 bp    DNA     circular  SYN 
DEFINITION  USP19 (Myc-DDK tagged) - Homo sapiens ubiquitin specific peptidase 19 (USP19), transcript variant 2
ACCESSION   NM_001199161
VERSION     NM_001199161.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5197..5288 
                     /label="Myc-DDK"  
     misc_feature    5318..5903 
                     /label="PolyA signal"  
     misc_feature    5375..5394 
                     /label="Restriction Enzyme"  
     misc_feature    6179..6851 
                     /label="ColE1"  
     misc_feature    7281..8075 
                     /label="Kan/Neo"  
     misc_feature    8110..8412 
                     /label="SV40 ori"  
     misc_feature    8498..8975 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5172..5179 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5172 
                     /label="USP19"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCTGGCGGG GCCAGTGCCA CAGGCCCAAG GAGAGGGCCC CCAGGACTGG 
     1081 AGGACACCAC TAGTAAGAAG AAGCAGAAGG ATCGAGCAAA CCAGGAGAGC AAGGATGGAG 
     1141 ATCCTAGGAA AGAGACAGGG TCTCGATATG TTGCCCAGGC TGGTCTTGAA CCTCTGGCCT 
     1201 CAGGTGATCC TTCTGCCTCA GCCTCCCATG CAGCTGGGAT CACAGGCTCA CGCCACCGTA 
     1261 CCCGGCTGTT CTTTCCTTCA TCGTCAGGGT CAGCATCCAC TCCTCAAGAG GAGCAGACCA 
     1321 AAGAGGGAGC TTGTGAAGAC CCTCATGATC TCTTGGCTAC TCCCACTCCA GAGTTGTTGC 
     1381 TCGATTGGAG GCAGAGTGCA GAAGAGGTGA TTGTCAAGCT TCGTGTGGGA GTAGGTCCCC 
     1441 TGCAGCTGGA GGATGTAGAT GCTGCTTTCA CAGATACAGA CTGTGTGGTG CGGTTTGCAG 
     1501 GTGGTCAGCA GTGGGGTGGT GTCTTCTATG CTGAGATAAA AAGCTCTTGT GCTAAAGTGC 
     1561 AAACCCGCAA GGGCAGTCTC CTGCACCTGA CACTGCCCAA AAAGGTGCCT ATGCTCACGT 
     1621 GGCCCTCCCT CCTGAAGAAA CCTCTAGGGA CCCAGGAGCT GGTGCCGGGG CTGCGGTGCC 
     1681 AGGAGAATGG GCAGGAACTG TCTCCCATTG CCCTGGAGCC AGGCCCTGAG CCCCACCGGG 
     1741 CTAAGCAGGA GGCCCGGAAC CAGAAGCGGG CCCAGGGCCG TGGTGAGGTA GGCGCAGGGG 
     1801 CTGGCCCCGG GGCCCAGGCA GGGCCCAGCG CCAAGAGGGC TGTGCATCTC TGCAGAGGGC 
     1861 CAGAGGGGGA CGGGTCCAGG GATGACCCTG GACCCCGGGG TGATGCCCCA CCCTTCGTGG 
     1921 CTGACCCAGC CACCCAGGTT GAGGCTGATG AACAGCTTTG CATACCACCG CTGAACTCCC 
     1981 AAACCTGCCT CCTGGGCTCA GAGGAGAATT TAGCCCCTTT GGCAGGAGAG AAAGCAGTGC 
     2041 CTCCCGGGAA TGACCCAGTC TCTCCAGCCA TGGTCCGGAG CAGAAACCCT GGGAAAGATG 
     2101 ACTGTGCCAA GGAGGAGATG GCAGTGGCAG CAGATGCTGC AACCTTGGTG GATGAGCCCG 
     2161 AGTCGATGGT GAACCTGGCG TTTGTCAAGA ATGACTCGTA TGAGAAGGGC CCGGATTCAG 
     2221 TGGTGGTGCA CGTGTACGTG AAGGAGATCT GCAGGGACAC CTCAAGAGTA CTTTTCCGTG 
     2281 AGCAGGACTT CACGCTCATC TTCCAGACCA GGGATGGAAA CTTCCTGAGG CTGCACCCGG 
     2341 GCTGTGGGCC CCACACCACC TTCCGTTGGC AGGTGAAGCT CAGGAATCTG ATTGAGCCAG 
     2401 AGCAGTGCAC CTTCTGTTTC ACGGCTTCTC GCATCGACAT CTGCCTTCGT AAGAGGCAGA 
     2461 GTCAGCGCTG GGGGGGCCTG GAGGCCCCGG CTGCACGAGG TGCAGTGGGT GGTGCAAAGG 
     2521 TTGCCGTGCC GACAGGTCCA ACCCCTCTGG ATTCAACCCC ACCAGGAGGT GCTCCCCACC 
     2581 CCCTGACAGG CCAGGAGGAG GCCCGGGCTG TGGAGAAGGA TAAATCCAAG GCACGATCTG 
     2641 AGGACACAGG GCTAGACAGT GTGGCAACCC GCACACCCAT GGAGCATGTA ACCCCAAAGC 
     2701 CAGAGACACA CCTGGCCTCG CCCAAGCCTA CATGCATGGT GCCTCCCATG CCCCACAGCC 
     2761 CAGTTAGTGG AGACAGCGTG GAGGAGGAGG AAGAGGAAGA GAAGAAGGTG TGTCTGCCAG 
     2821 GCTTCACTGG CCTTGTCAAT TTAGGCAACA CCTGCTTCAT GAACAGCGTC ATTCAGTCTC 
     2881 TGTCCAACAC TCGGGAACTC CGGGACTTCT TCCATGACCG CTCCTTTGAG GCTGAGATCA 
     2941 ACTACAACAA CCCACTAGGG ACTGGTGGGC GTCTGGCCAT TGGCTTTGCC GTGCTGCTTC 
     3001 GGGCGCTGTG GAAGGGCACC CACCATGCCT TCCAGCCTTC CAAGTTGAAG GCCATTGTGG 
     3061 CGAGTAAGGC CAGCCAGTTC ACAGGCTATG CACAGCATGA TGCCCAGGAG TTCATGGCTT 
     3121 TCCTGCTGGA TGGGCTGCAC GAGGACCTGA ATCGCATTCA GAACAAGCCC TACACAGAGA 
     3181 CCGTGGATTC AGATGGGCGG CCCGATGAGG TGGTAGCTGA GGAAGCATGG CAGCGGCACA 
     3241 AGATGAGGAA TGACTCTTTC ATCGTGGACC TATTTCAGGG GCAGTACAAG TCGAAGCTGG 
     3301 TGTGCCCTGT GTGTGCCAAG GTCTCCATCA CTTTTGACCC GTTTCTTTAT CTGCCGGTGC 
     3361 CCTTGCCACA AAAGCAAAAG GTTCTCCCTG TCTTTTATTT TGCCCGAGAG CCCCACAGCA 
     3421 AGCCCATCAA GTTCCTGGTG AGCGTCAGCA AGGAGAACTC CACTGCGAGC GAAGTATTGG 
     3481 ACTCCCTCTC TCAGAGTGTT CATGTGAAGC CTGAGAACCT GCGTTTGGCG GAGGTAATTA 
     3541 AGAATCGTTT TCATCGTGTG TTCCTACCCT CCCACTCACT GGACACTGTG TCCCCATCTG 
     3601 ATACGCTCCT CTGCTTTGAG CTGCTATCCT CAGAGTTGGC TAAGGAGCGG GTAGTGGTGC 
     3661 TAGAGGTGCA ACAGCGCCCC CAGGTGCCCA GCGTCCCCAT CTCCAAGTGT GCAGCCTGCC 
     3721 AGCGGAAGCA ACAGTCGGAG GATGAAAAGC TGAAGCGCTG TACCCGGTGC TACCGTGTGG 
     3781 GCTACTGCAA CCAGCTCTGC CAGAAAACCC ACTGGCCTGA CCACAAGGGC CTCTGCCGAC 
     3841 CTGAGAACAT TGGCTACCCC TTCCTGGTCA GTGTACCTGC CTCACGCCTC ACTTATGCCC 
     3901 GCCTCGCTCA GTTGCTAGAG GGCTATGCCC GGTACTCTGT GAGTGTATTC CAGCCACCCT 
     3961 TTCAGCCAGG CCGCATGGCC TTGGAGTCTC AGAGCCCTGG CTGCACCACA CTGCTCTCCA 
     4021 CAGGTTCCCT GGAGGCTGGG GACAGCGAGA GAGACCCCAT TCAGCCACCT GAGCTCCAGC 
     4081 TGGTGACCCC TATGGCTGAG GGGGACACAG GGCTTCCCCG GGTGTGGGCA GCCCCTGACC 
     4141 GGGGTCCTGT GCCCAGCACC AGTGGAATTT CTTCTGAGAT GCTGGCCAGT GGGCCCATTG 
     4201 AGGTTGGCTC CTTGCCAGCT GGCGAGAGGG TGTCCCGACC CGAAGCTGCT GTGCCTGGGT 
     4261 ACCAGCATCC AAGTGAAGCT ATGAATGCCC ACACACCCCA GTTCTTCATC TATAAAATTG 
     4321 ATTCATCCAA CCGAGAGCAG CGGCTAGAGG ACAAAGGAGA CACCCCACTG GAGCTGGGTG 
     4381 ACGACTGTAG CCTGGCTCTC GTCTGGCGGA ACAATGAGCG CTTGCAGGAG TTTGTGTTGG 
     4441 TAGCCTCCAA GGAGCTGGAA TGTGCTGAGG ATCCAGGCTC TGCCGGTGAG GCTGCCCGGG 
     4501 CCGGCCACTT CACCCTGGAC CAGTGCCTCA ACCTCTTCAC ACGGCCTGAG GTGCTGGCAC 
     4561 CCGAGGAGGC CTGGTACTGC CCACAGTGCA AACAGCACCG TGAGGCCTCC AAGCAGCTGT 
     4621 TGCTATGGCG CCTGCCAAAT GTTCTCATCG TGCAGCTCAA GCGCTTCTCC TTTCGTAGTT 
     4681 TTATCTGGCG TGACAAGATC AATGACTTGG TGGAGTTCCC TGTTAGGAAC CTGGACCTGA 
     4741 GCAAGTTCTG CATTGGTCAG AAAGAGGAGC AGCTGCCCAG CTACGATCTA TATGCTGTCA 
     4801 TCAACCACTA TGGAGGCATG ATTGGTGGCC ACTACACTGC CTGTGCACGC CTGCCCAATG 
     4861 ATCGTAGCAG TCAGCGCAGT GACGTGGGCT GGCGCTTGTT TGATGACAGC ACAGTGACAA 
     4921 CGGTAGACGA GAGCCAGGTT GTGACGCGTT ATGCCTATGT ACTCTTCTAC CGCCGGCGGA 
     4981 ACTCTCCTGT GGAGAGGCCC CCCAGGGCAG GTCACTCTGA GCACCACCCA GACCTAGGCC 
     5041 CTGCAGCTGA GGCTGCTGCC AGCCAGGGAC TAGGCCCTGG CCAGGCCCCC GAGGTGGCCC 
     5101 CCACGCGGAC AGCCCCTGAA CGCTTCGCCC CCCCTGTGGA TCGGCCAGCC CCCACCTACA 
     5161 GCAACATGGA GGAGGTGGAT AGCGGACCGA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     5221 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     5281 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     5341 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     5401 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     5461 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     5521 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     5581 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     5641 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     5701 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     5761 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     5821 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     5881 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     5941 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     6001 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     6061 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     6121 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     6181 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     6241 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     6301 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     6361 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     6421 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     6481 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     6541 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     6601 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     6661 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     6721 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     6781 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     6841 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     6901 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     6961 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     7021 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     7081 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     7141 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     7201 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     7261 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     7321 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     7381 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     7441 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     7501 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     7561 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     7621 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     7681 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     7741 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     7801 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     7861 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     7921 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     7981 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     8041 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     8101 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     8161 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     8221 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     8281 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     8341 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     8401 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     8461 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     8521 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     8581 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     8641 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     8701 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     8761 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     8821 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     8881 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     8941 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     9001 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//