LOCUS       RC235284                9006 bp    DNA     circular  SYN 
DEFINITION  USP19 (Myc-DDK tagged) - Homo sapiens ubiquitin specific peptidase 19 (USP19), transcript variant 3
ACCESSION   NM_001199162
VERSION     NM_001199162.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5161..5252 
                     /label="Myc-DDK"  
     misc_feature    5282..5867 
                     /label="PolyA signal"  
     misc_feature    5339..5358 
                     /label="Restriction Enzyme"  
     misc_feature    6143..6815 
                     /label="ColE1"  
     misc_feature    7245..8039 
                     /label="Kan/Neo"  
     misc_feature    8074..8376 
                     /label="SV40 ori"  
     misc_feature    8462..8939 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5136..5143 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5136 
                     /label="USP19"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCTGGCGGG GCCAGTGCCA CAGGCCCAAG GAGAGGGCCC CCAGGACTGG 
     1081 AGGACACCAC TAGTAAGAAG AAGCAGAAGG ATCGAGCAAA CCAGGAGAGC AAGGATGGAG 
     1141 ATCCTAGGAA AGAGACAGGG TCTCGATATG TTGCCCAGGC TGGTCTTGAA CCTCTGGCCT 
     1201 CAGGTGATCC TTCTGCCTCA GCCTCCCATG CAGCTGGGAT CACAGGCTCA CGCCACCGTA 
     1261 CCCGGCTGTT CTTTCCTTCA TCGTCAGGGT CAGCATCCAC TCCTCAAGAG GAGCAGACCA 
     1321 AAGAGGGAGC TTGTGAAGAC CCTCATGATC TCTTGGCTAC TCCCACTCCA GAGTTGTTGC 
     1381 TCGATTGGAG GCAGAGTGCA GAAGAGGTGA TTGTCAAGCT TCGTGTGGGA GTAGGTCCCC 
     1441 TGCAGCTGGA GGATGTAGAT GCTGCTTTCA CAGATACAGA CTGTGTGGTG CGGTTTGCAG 
     1501 GTGGTCAGCA GTGGGGTGGT GTCTTCTATG CTGAGATAAA AAGCTCTTGT GCTAAAGTGC 
     1561 AAACCCGCAA GGGCAGTCTC CTGCACCTGA CACTGCCCAA AAAGAAGAAA CCTCTAGGGA 
     1621 CCCAGGAGCT GGTGCCGGGG CTGCGGTGCC AGGAGAATGG GCAGGAACTG TCTCCCATTG 
     1681 CCCTGGAGCC AGGCCCTGAG CCCCACCGGG CTAAGCAGGA GGCCCGGAAC CAGAAGCGGG 
     1741 CCCAGGGCCG TGGTGAGGTA GGCGCAGGGG CTGGCCCCGG GGCCCAGGCA GGGCCCAGCG 
     1801 CCAAGAGGGC TGTGCATCTC TGCAGAGGGC CAGAGGGGGA CGGGTCCAGG GATGACCCTG 
     1861 GACCCCGGGG TGATGCCCCA CCCTTCGTGG CTGACCCAGC CACCCAGGTT GAGGCTGATG 
     1921 AACAGCTTTG CATACCACCG CTGAACTCCC AAACCTGCCT CCTGGGCTCA GAGGAGAATT 
     1981 TAGCCCCTTT GGCAGGAGAG AAAGCAGTGC CTCCCGGGAA TGACCCAGTC TCTCCAGCCA 
     2041 TGGTCCGGAG CAGAAACCCT GGGAAAGATG ACTGTGCCAA GGAGGAGATG GCAGTGGCAG 
     2101 CAGATGCTGC AACCTTGGTG GATGAGCCCG AGTCGATGGT GAACCTGGCG TTTGTCAAGA 
     2161 ATGACTCGTA TGAGAAGGGC CCGGATTCAG TGGTGGTGCA CGTGTACGTG AAGGAGATCT 
     2221 GCAGGGACAC CTCAAGAGTA CTTTTCCGTG AGCAGGACTT CACGCTCATC TTCCAGACCA 
     2281 GGGATGGAAA CTTCCTGAGG CTGCACCCGG GCTGTGGGCC CCACACCACC TTCCGTTGGC 
     2341 AGGTGAAGCT CAGGAATCTG ATTGAGCCAG AGCAGTGCAC CTTCTGTTTC ACGGCTTCTC 
     2401 GCATCGACAT CTGCCTTCGT AAGAGGCAGA GTCAGCGCTG GGGGGGCCTG GAGGCCCCGG 
     2461 CTGCACGAGT GGGTGGTGCA AAGGTTGCCG TGCCGACAGG TCCAACCCCT CTGGATTCAA 
     2521 CCCCACCAGG AGGTGCTCCC CACCCCCTGA CAGGCCAGGA GGAGGCCCGG GCTGTGGAGA 
     2581 AGGATAAATC CAAGGCACGA TCTGAGGACA CAGGGCTAGA CAGTGTGGCA ACCCGCACAC 
     2641 CCATGGAGCA TGTAACCCCA AAGCCAGAGA CACACCTGGC CTCGCCCAAG CCTACATGCA 
     2701 TGGTGCCTCC CATGCCCCAC AGCCCAGTTA GTGGAGACAG CGTGGAGGAG GAGGAAGAGG 
     2761 AAGAGAAGAA GGTGTGTCTG CCAGGCTTCA CTGGCCTTGT CAATTTAGGC AACACCTGCT 
     2821 TCATGAACAG CGTCATTCAG TCTCTGTCCA ACACTCGGGA ACTCCGGGAC TTCTTCCATG 
     2881 ACCGCTCCTT TGAGGCTGAG ATCAACTACA ACAACCCACT AGGGACTGGT GGGCGTCTGG 
     2941 CCATTGGCTT TGCCGTGCTG CTTCGGGCGC TGTGGAAGGG CACCCACCAT GCCTTCCAGC 
     3001 CTTCCAAGTT GAAGGCCATT GTGGCGAGTA AGGCCAGCCA GTTCACAGGC TATGCACAGC 
     3061 ATGATGCCCA GGAGTTCATG GCTTTCCTGC TGGATGGGCT GCACGAGGAC CTGAATCGCA 
     3121 TTCAGAACAA GCCCTACACA GAGACCGTGG ATTCAGATGG GCGGCCCGAT GAGGTGGTAG 
     3181 CTGAGGAAGC ATGGCAGCGG CACAAGATGA GGAATGACTC TTTCATCGTG GACCTATTTC 
     3241 AGGGGCAGTA CAAGTCGAAG CTGGTGTGCC CTGTGTGTGC CAAGGTCTCC ATCACTTTTG 
     3301 ACCCGTTTCT TTATCTGCCG GTGCCCTTGC CACAAAAGCA AAAGGTTCTC CCTGTCTTTT 
     3361 ATTTTGCCCG AGAGCCCCAC AGCAAGCCCA TCAAGTTCCT GGTGAGCGTC AGCAAGGAGA 
     3421 ACTCCACTGC GAGCGAAGTA TTGGACTCCC TCTCTCAGAG TGTTCATGTG AAGCCTGAGA 
     3481 ACCTGCGTTT GGCGGAGGTA ATTAAGAATC GTTTTCATCG TGTGTTCCTA CCCTCCCACT 
     3541 CACTGGACAC TGTGTCCCCA TCTGATACGC TCCTCTGCTT TGAGCTGCTA TCCTCAGAGT 
     3601 TGGCTAAGGA GCGGGTAGTG GTGCTAGAGG TGCAACAGCG CCCCCAGGTG CCCAGCGTCC 
     3661 CCATCTCCAA GTGTGCAGCC TGCCAGCGGA AGCAACAGTC GGAGGATGAA AAGCTGAAGC 
     3721 GCTGTACCCG GTGCTACCGT GTGGGCTACT GCAACCAGCT CTGCCAGAAA ACCCACTGGC 
     3781 CTGACCACAA GGGCCTCTGC CGACCTGAGA ACATTGGCTA CCCCTTCCTG GTCAGTGTAC 
     3841 CTGCCTCACG CCTCACTTAT GCCCGCCTCG CTCAGTTGCT AGAGGGCTAT GCCCGGTACT 
     3901 CTGTGAGTGT ATTCCAGCCA CCCTTTCAGC CAGGCCGCAT GGCCTTGGAG TCTCAGAGCC 
     3961 CTGGCTGCAC CACACTGCTC TCCACAGGTT CCCTGGAGGC TGGGGACAGC GAGAGAGACC 
     4021 CCATTCAGCC ACCTGAGCTC CAGCTGGTGA CCCCTATGGC TGAGGGGGAC ACAGGGCTTC 
     4081 CCCGGGTGTG GGCAGCCCCT GACCGGGGTC CTGTGCCCAG CACCAGTGGA ATTTCTTCTG 
     4141 AGATGCTGGC CAGTGGGCCC ATTGAGGTTG GCTCCTTGCC AGCTGGCGAG AGGGTGTCCC 
     4201 GACCCGAAGC TGCTGTGCCT GGGTACCAGC ATCCAAGTGA AGCTATGAAT GCCCACACAC 
     4261 CCCAGTTCTT CATCTATAAA ATTGATTCAT CCAACCGAGA GCAGCGGCTA GAGGACAAAG 
     4321 GAGACACCCC ACTGGAGCTG GGTGACGACT GTAGCCTGGC TCTCGTCTGG CGGAACAATG 
     4381 AGCGCTTGCA GGAGTTTGTG TTGGTAGCCT CCAAGGAGCT GGAATGTGCT GAGGATCCAG 
     4441 GCTCTGCCGG TGAGGCTGCC CGGGCCGGCC ACTTCACCCT GGACCAGTGC CTCAACCTCT 
     4501 TCACACGGCC TGAGGTGCTG GCACCCGAGG AGGCCTGGTA CTGCCCACAG TGCAAACAGC 
     4561 ACCGTGAGGC CTCCAAGCAG CTGTTGCTAT GGCGCCTGCC AAATGTTCTC ATCGTGCAGC 
     4621 TCAAGCGCTT CTCCTTTCGT AGTTTTATCT GGCGTGACAA GATCAATGAC TTGGTGGAGT 
     4681 TCCCTGTTAG GAACCTGGAC CTGAGCAAGT TCTGCATTGG TCAGAAAGAG GAGCAGCTGC 
     4741 CCAGCTACGA TCTATATGCT GTCATCAACC ACTATGGAGG CATGATTGGT GGCCACTACA 
     4801 CTGCCTGTGC ACGCCTGCCC AATGATCGTA GCAGTCAGCG CAGTGACGTG GGCTGGCGCT 
     4861 TGTTTGATGA CAGCACAGTG ACAACGGTAG ACGAGAGCCA GGTTGTGACG CGTTATGCCT 
     4921 ATGTACTCTT CTACCGCCGG CGGAACTCTC CTGTGGAGAG GCCCCCCAGG GCAGGTCACT 
     4981 CTGAGCACCA CCCAGACCTA GGCCCTGCAG CTGAGGCTGC TGCCAGCCAG GGACTAGGCC 
     5041 CTGGCCAGGC CCCCGAGGTG GCCCCCACGC GGACAGCCCC TGAACGCTTC GCCCCCCCTG 
     5101 TGGATCGGCC AGCCCCCACC TACAGCAACA TGGAGGAGGT GGATAGCGGA CCGACGCGTA 
     5161 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     5221 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     5281 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     5341 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     5401 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     5461 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     5521 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     5581 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     5641 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     5701 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     5761 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     5821 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5881 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     5941 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     6001 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     6061 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     6121 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     6181 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     6241 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     6301 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     6361 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     6421 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     6481 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     6541 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     6601 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     6661 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     6721 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     6781 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     6841 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     6901 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     6961 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     7021 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     7081 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     7141 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     7201 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     7261 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     7321 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     7381 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     7441 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     7501 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     7561 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     7621 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     7681 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     7741 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     7801 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     7861 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     7921 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     7981 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     8041 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     8101 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     8161 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     8221 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     8281 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     8341 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     8401 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     8461 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     8521 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     8581 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     8641 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     8701 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     8761 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     8821 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     8881 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     8941 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     9001 AATTTT
//