LOCUS       RC235268                8871 bp    DNA     circular  SYN 
DEFINITION  PASK (Myc-DDK tagged) - Homo sapiens PAS domain containing serine/threonine kinase (PASK), transcript variant 1
ACCESSION   NM_001252119
VERSION     NM_001252119.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5028..5119 
                     /label="Myc-DDK"  
     misc_feature    5149..5734 
                     /label="PolyA signal"  
     misc_feature    5206..5225 
                     /label="Restriction Enzyme"  
     misc_feature    6010..6682 
                     /label="ColE1"  
     misc_feature    7112..7906 
                     /label="Kan/Neo"  
     misc_feature    7941..8243 
                     /label="SV40 ori"  
     misc_feature    8329..8806 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5010..5016 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5010 
                     /label="PASK"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGGACGGG GGCTTAACAG CCTTTGAAGA GGACCAGAGA TGCCTTTCCC 
     1081 AGAGCCTCCC CTTGCCAGTG TCAGCAGAGG GCCCAGCTGC ACAGACCACT GCTGAGCCCA 
     1141 GCAGGTCGTT TTCCTCAGCC CACAGACACC TGAGCAGAAG GAATGGGCTT TCCAGACTCT 
     1201 GCCAGAGCAG GACAGCGCTC TCTGAAGACA GATGGAGCTC CTATTGTCTA TCATCACTGG 
     1261 CTGCCCAGAA TATTTGTACA AGTAAACTGC ACTGCCCTGC TGCCCCTGAG CACACGGACC 
     1321 CGTCCGAACC GCGGGGCAGT GTGTCCTGCT GCTCCCTGCT GCGGGGACTG TCCTCAGGGT 
     1381 GGTCCTCACC TCTGCTTCCG GCCCCTGTGT GCAACCCTAA CAAGGCCATC TTCACGGTGG 
     1441 ATGCCAAGAC CACAGAGATC CTGGTTGCTA ACGACAAAGC TTGCGGGCTC CTGGGGTACA 
     1501 GCAGCCAGGA CCTGATTGGC CAGAAGCTCA CGCAGTTCTT TCTGAGGTCA GATTCTGATG 
     1561 TGGTGGAGGC CCTCAGCGAG GAGCACATGG AGGCCGACGG CCACGCTGCG GTGGTGTTTG 
     1621 GCACGGTGGT GGACATCATC AGCCGTAGTG GGGAGAAGAT TCCAGTGTCT GTGTGGATGA 
     1681 AGAGGATGCG GCAGGAGCGC CGCCTATGCT GCGTGGTGGT CCTGGAGCCC GTGGAGAGGG 
     1741 TCTCGACCTG GGTCGCTTTC CAGAGCGATG GCACCGTCAC GTCATGTGAC AGTCTCTTTG 
     1801 CTCATCTTCA CGGGTACGTG TCTGGGGAGG ACGTGGCTGG GCAGCATATC ACAGACCTGA 
     1861 TCCCTTCTGT GCAGCTCCCT CCTTCTGGCC AGCACATCCC AAAGAATCTC AAGATTCAGA 
     1921 GGTCTGTTGG AAGAGCCAGG GACGGTACCA CCTTCCCTCT GAGCTTAAAG CTGAAATCCC 
     1981 AACCCAGCAG CGAGGAGGCG ACCACCGGTG AGGCGGCCCC TGTGAGCGGC TACCGGGCAT 
     2041 CTGTCTGGGT GTTCTGCACC ATCAGTGGCC TCATCACCCT CCTGCCGGAT GGGACCATCC 
     2101 ACGGCATCAA CCACAGCTTC GCGCTGACAC TGTTTGGTTA CGGAAAGACG GAGCTCCTGG 
     2161 GCAAGAATAT CACTTTCCTG ATTCCTGGTT TCTACAGCTA CATGGACCTT GCGTACAACA 
     2221 GCTCATTACA GCTCCCAGAC CTGGCCAGCT GCCTGGACGT CGGCAATGAG AGTGGGTGTG 
     2281 GGGAGAGAAC CTTGGACCCG TGGCAGGGCC AGGACCCAGC TGAGGGGGGC CAGGATCCAA 
     2341 GGATTAATGT CGTGCTTGCT GGTGGCCACG TTGTGCCCCG AGATGAGATC CGGAAGCTGA 
     2401 TGGAAAGCCA AGACATCTTC ACCGGGACTC AGACTGAGCT GATTGCTGGA GGCCAGCTCC 
     2461 TTTCCTGCCT CTCACCTCAG CCTGCTCCAG GGGTGGACAA TGTCCCAGAA GGAAGCCTGC 
     2521 CAGTGCACGG TGAACAGGCG CTGCCCAAGG ACCAGCAAAT CACTGCCTTG GGGAGAGAGG 
     2581 AACCTGTGGC AATAGAGAGC CCCGGACAGG ATCTTCTGGG AGAAAGCAGG TCTGAACCAG 
     2641 TGGATGTGAA GCCATTTGCT TCCTGCGAAG ATTCTGAAGC TCCAGTCCCA GCTGAGGATG 
     2701 GGGGCAGTGA TGCTGGCATG TGTGGCCTGT GTCAGAAGGC CCAGCTAGAG CGGATGGGAG 
     2761 TCAGTGGTCC CAGCGGTTCA GACCTTTGGG CTGGGGCTGC CGTGGCCAAG CCCCAGGCCA 
     2821 AGGGTCAGCT GGCGGGGGGC AGCCTCCTGA TGCACTGCCC TTGCTATGGG AGTGAATGGG 
     2881 GCTTGTGGTG GCGAAGCCAG GACTTGGCCC CCAGCCCCTC TGGGATGGCA GGCCTCTCGT 
     2941 TTGGGACACC TACTCTAGAT GAGCCGTGGC TGGGAGTGGA AAACGACCGA GAAGAGCTGC 
     3001 AGACCTGCTT GATTAAGGAG CAGCTGTCCC AGTTGAGCCT TGCAGGAGCC CTGGATGTCC 
     3061 CCCACGCCGA ACTCGTTCCG ACAGAGTGCC AGGCTGTCAC CGCTCCTGTG TCGTCCTGCG 
     3121 ATCTGGGAGG CAGAGACCTG TGCGGTGGCT GCACGGGCAG CTCCTCAGCC TGCTATGCCT 
     3181 TGGCCACGGA CCTCCCTGGG GGCCTGGAAG CAGTGGAGGC CCAGGAGGTT GATGTGAATT 
     3241 CGTTTTCCTG GAACCTCAAG GAACTCTTTT TCAGTGACCA GACAGACCAA ACGTCATCAA 
     3301 ATTGTTCCTG TGCTACGTCT GAACTCAGAG AGACACCCTC TTCCTTGGCA GTGGGCTCCG 
     3361 ATCCAGATGT AGGCAGTCTC CAGGAACAGG GGTCGTGTGT CCTGGATGAC AGGGAGCTGT 
     3421 TACTACTGAC CGGCACCTGT GTTGACCTTG GCCAAGGCCG ACGGTTCCGG GAGAGCTGTG 
     3481 TGGGACATGA TCCAACAGAA CCGCTTGAGG TTTGTTTGGT GTCCTCTGAG CATTATGCAG 
     3541 CAAGCGACAG AGAAAGCCCA GGACACGTTC CTTCCACGTT GGATGCTGGC CCTGAGGACA 
     3601 CGTGCCCATC AGCAGAGGAG CCAAGGCTGA ACGTCCAGGT CACCTCCACG CCCGTGATCG 
     3661 TGATGCGCGG GGCTGCTGGC CTGCAGCGGG AGATCCAGGA GGGTGCCTAC TCCGGGAGCT 
     3721 GCTACCATCG AGATGGCTTA CGGCTGAGTA TACAGTTTGA GGTGAGGCGG GTGGAGCTCC 
     3781 AGGGCCCCAC ACCTCTGTTC TGCTGCTGGC TGGTGAAAGA CCTCCTCCAC AGCCAACGCG 
     3841 ACTCAGCCGC CAGGACCCGC CTGTTCCTTG CCAGCCTGCC CGGCTCCACC CACTCTACCG 
     3901 CTGCTGAGCT CACCGGACCC AGCCTGGTGG AAGTGCTCAG AGCCAGACCC TGGTTTGAGG 
     3961 AGCCCCCCAA GGCTGTGGAA CTGGAGGGGT TGGCGGCCTG TGAGGGCGAG TACTCCCAAA 
     4021 AGTACAGTAC CATGAGCCCG CTGGGCAGTG GGGCCTTCGG CTTCGTGTGG ACTGCTGTGG 
     4081 ACAAGGAAAA AAACAAGGAG GTGGTGGTGA AGTTTATTAA GAAGGAGAAG GTCTTGGAGG 
     4141 ATTGTTGGAT TGAGGATCCC AAACTTGGGA AAGTTACTTT AGAGATCGCA ATTCTATCCA 
     4201 GGGTGGAGCA CGCCAATATC ATCAAGGTAT TGGATATATT TGAAAACCAA GGGTTCTTCC 
     4261 AGCTTGTGAT GGAGAAGCAC GGCTCCGGCC TAGACCTCTT CGCTTTCATC GACCGCCACC 
     4321 CCAGGCTGGA TGAGCCCCTG GCGAGCTACA TCTTCCGACA AGTGAGAGCA GGCCAGAGCC 
     4381 GTCTAGTGTC AGCAGTGGGA TACCTGCGCT TGAAGGACAT CATCCACCGT GACATCAAGG 
     4441 ATGAGAACAT CGTGATCGCC GAGGACTTCA CAATCAAGCT GATAGACTTT GGCTCGGCCG 
     4501 CCTACTTGGA AAGGGGAAAA TTATTTTATA CTTTTTGTGG GACCATCGAG TACTGTGCAC 
     4561 CGGAAGTTCT CATGGGGAAT CCCTACAGAG GGCCGGAGCT GGAGATGTGG TCTCTGGGAG 
     4621 TCACTCTGTA CACGCTGGTC TTTGAGGAGA ACCCCTTCTG TGAGCTGGAG GAGACCGTGG 
     4681 AGGCTGCCAT ACACCCGCCA TACCTGGTGT CCAAAGAACT CATGAGCCTT GTGTCTGGGC 
     4741 TGCTGCAGCC AGTCCCTGAG AGACGCACCA CCTTGGAGAA GCTGGTGACA GACCCGTGGG 
     4801 TAACACAGCC TGTGAATCTT GCTGACTATA CATGGGAAGA GGTGTTTCGA GTAAACAAGC 
     4861 CAGAAAGTGG AGTTCTGTCC GCTGCGAGCC TGGAGATGGG GAACAGGAGC CTGAGTGATG 
     4921 TGGCCCAGGC TCAGGAGCTT TGTGGGGGCC CCGTTCCAGG CGAGGCTCCT AATGGCCAAG 
     4981 GCTGTTTGCA TCCCGGGGAT CCCCGTCTGC TGACCAGCAC GCGTACGCGG CCGCTCGAGC 
     5041 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     5101 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     5161 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     5221 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     5281 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     5341 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     5401 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     5461 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     5521 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     5581 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     5641 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     5701 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5761 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     5821 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     5881 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     5941 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     6001 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     6061 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     6121 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     6181 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     6241 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     6301 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     6361 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     6421 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     6481 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     6541 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     6601 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     6661 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     6721 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     6781 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     6841 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     6901 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     6961 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     7021 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     7081 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     7141 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     7201 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     7261 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     7321 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     7381 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     7441 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     7501 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     7561 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     7621 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     7681 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     7741 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     7801 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     7861 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     7921 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     7981 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     8041 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     8101 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     8161 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     8221 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     8281 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     8341 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     8401 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     8461 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     8521 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     8581 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     8641 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     8701 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     8761 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     8821 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
//