LOCUS       RC235258                8784 bp    DNA     circular  SYN 
DEFINITION  AMBRA1 (Myc-DDK tagged) - Homo sapiens autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant 1
ACCESSION   NM_001267782
VERSION     NM_001267782.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4941..5032 
                     /label="Myc-DDK"  
     misc_feature    5062..5647 
                     /label="PolyA signal"  
     misc_feature    5119..5138 
                     /label="Restriction Enzyme"  
     misc_feature    5923..6595 
                     /label="ColE1"  
     misc_feature    7025..7819 
                     /label="Kan/Neo"  
     misc_feature    7854..8156 
                     /label="SV40 ori"  
     misc_feature    8242..8719 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4923..4929 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4923 
                     /label="AMBRA1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGGTTGTC CCAGAAAAGA ATGCTGTCCG GATACTCTGG GGGCGAGAAC 
     1081 GGGGTGCTCG GGCCATGGGA GCTCAGCGGC TTCTGCAGGA GCTGGTAGAA GATAAAACCC 
     1141 GGTGGATGAA ATGGGAGGGC AAGAGAGTAG AACTGCCGGA TAGTCCACGC TCTACCTTCT 
     1201 TATTGGCCTT CAGCCCAGAC AGGACTCTCT TAGCCTCCAC CCATGTGAAC CATAATATCT 
     1261 ATATTACGGA GGTGAAGACT GGCAAGTGTG TTCATTCCCT GATTGGACAC CGCCGTACTC 
     1321 CATGGTGTGT CACTTTTCAT CCCACCATCT CAGGCCTTAT TGCTTCTGGC TGCCTAGATG 
     1381 GGGAGGTTAG GATTTGGGAT TTACACGGTG GCAGTGAAAG CTGGTTCACA GATAGCAACA 
     1441 ATGCCATTGC CTCCCTGGCT TTCCACCCTA CGGCTCAGCT CCTGCTGATT GCCACTGCCA 
     1501 ATGAGATCCA CTTCTGGGAC TGGAGTCGAC GGGAACCCTT TGCTGTGGTG AAGACAGCTA 
     1561 GTGAGATGGA ACGGGTCCGT CTGGTGAGAT TTGATCCACT TGGACACTAC TTACTCACAG 
     1621 CAATTGTTAA CCCCTCTAAT CAACAGGGTG ATGACGAACC AGAGATCCCC ATAGATGGAA 
     1681 CAGAATTATC CCACTACCGT CAGCGTGCCC TCCTGCAATC ACAGCCAGTT CGCCGGACGC 
     1741 CTCTCCTCCA CAATTTCCTG CACATGCTGT CCTCCCGCTC TTCTGGCATC CAGACCGAGC 
     1801 CCTTCCATCC CCCGGAGCAG GCCTCGTCAA CGCAGCAGGA CCAGGGCCTC CTGAACCGGC 
     1861 CGTCTGCCTT CAGTACAGTC CAGAGCAGCA CTGCCGGCAA CACGCTCCGC AACCTCAGTC 
     1921 TGGGTCCTAC CCGCCGCTCT TTGGGAGGGC CTCTGTCTAG CCACCCTTCT AGGTATCACC 
     1981 GAGAAATAGC TCCTGGGTTG ACAGGATCTG AGTGGACCCG GACAGTACTC AGTCTGAACT 
     2041 CCCGCTCTGA GGCGGAATCC ATGCCCCCGC CCAGAACCAG TGCCTCTTCG GTGAGTTTGC 
     2101 TGTCTGTGCT GAGACAGCAG GAAGGTGGCT CTCAGGCATC TGTGTACACT TCAGCCACAG 
     2161 AAGGGAGGGG TTTTCCGGCA TCAGGGTTGG CAACTGAGTC AGATGGAGGG AATGGCTCCA 
     2221 GCCAAAACAA CTCGGGCAGC ATTCGCCATG AGCTTCAGTG TGACCTGAGA CGCTTCTTTC 
     2281 TGGAGTATGA CCGGCTTCAG GAGCTGGATC AGAGCCTGAG TGGGGAAGCT CCCCAGACCC 
     2341 AACAGGCCCA GGAAATGCTC AACAATAACA TTGAATCTGA GAGGCCAGGC CCTTCCCACC 
     2401 AGCCCACCCC ACACAGCAGT GAGAACAACT CCAACCTGTC CCGTGGCCAC CTGAATCGCT 
     2461 GTCGTGCTTG CCACAATCTC CTGACCTTCA ACAACGATAC CCTGCGCTGG GAAAGAACCA 
     2521 CACCTAACTA CTCCTCTGGC GAGGCTAGTT CCTCTTGGCA GGTCCCCAGC TCCTTTGAGA 
     2581 GTGTGCCATC AAGTGGCAGC CAGTTGCCAC CTCTCGAGCG GACTGAGGGC CAAACGCCCA 
     2641 GCTCCAGCAG GCTGGAGTTG AGCAGCTCTG CTAGTCCGCA GGAGGAGAGG ACTGTGGGGG 
     2701 TGGCCTTTAA CCAGGAGACA GGCCACTGGG AAAGAATTTA CACCCAGTCC AGCAGATCTG 
     2761 GAACTGTGTC ACAGGAGGCC TTACATCAGG ATATGCCTGA GGAGAGCTCT GAGGAGGATT 
     2821 CACTCAGGAG ACGGAGTCTT GCTCTGTCAC CTAGGCTGGA GTACAGCGGC GCTATCTTGG 
     2881 CTCACTGCAA GCTCCGCCTC CCAGGTTCAT GCCATTCTCC TGCCTCAGCT TCCCAAGTAG 
     2941 CTGGGACTAC GGGAGCCCAC CACCACGCCC GGCTAATTTT TGCATTTTTA GTAGAGATGG 
     3001 AGTTTCACCA TGTTAGCCAG GCTGGTCTCG AACTCCTGAC CTCAGGTGAT CTGCCCACCT 
     3061 CGGCCTCCCA AAGTGCTGGG ATTACAGGCG TGAGCCACCG CGCCTGGCCG AGGCTGCTGG 
     3121 AATCTTCCCT CATTTCATTA TCCCGTTATG ATGGAGCAGG ATCCAGAGAG CACCCAATTT 
     3181 ACCCAGACCC AGCGAGATTA TCTCCTGCTG CATACTACGC CCAGAGGATG ATCCAGTATC 
     3241 TCTCACGGAG AGACAGTATT CGCCAGCGCT CCATGCGCTA CCAACAGAAC CGTCTCCGTT 
     3301 CTTCCACCTC CTCCTCTTCC TCAGACAACC AGGGTCCATC AGTAGAGGGA ACCGACTTGG 
     3361 AATTTGAGGA CTTTGAGGAC AATGGTGACA GATCCAGGCA CCGAGCTCCA CGCAATGCCC 
     3421 GGATGTCTGC ACCTTCGCTT GGACGCTTTG TCCCAAGGCG TTTCTTGCTG CCTGAGTACT 
     3481 TGCCTTATGC TGGGATTTTT CATGAACGTG GACAGCCTGG CTTGGCTACT CACTCTTCTG 
     3541 TTAACAGGGT CCTGGCAGGG GCAGTGATCG GTGATGGACA GTCTGCTGTG GCCAGTAACA 
     3601 TTGCCAATAC TACCTACCGG CTCCAGTGGT GGGACTTCAC TAAGTTTGAC CTCCCTGAAA 
     3661 TCAGTAATGC TTCCGTGAAT GTGCTGGTGC AGAACTGCAA GATCTACAAT GATGCCAGCT 
     3721 GTGACATTTC TGCAGATGGC CAGCTCCTGG CAGCTTTCAT CCCCAGCAGC CAGAGGGGCT 
     3781 TTCCTGATGA AGGCATCCTG GCAGTGTACT CCCTGGCCCC CCATAACCTG GGCGAAATGC 
     3841 TCTACACCAA GCGATTTGGT CCCAATGCCA TTTCGGTGAG CCTGTCCCCA ATGGGCAGAT 
     3901 ATGTAATGGT GGGCTTGGCC TCACGAAGGA TCCTGCTGCA CCCCTCCACA GAGCACATGG 
     3961 TGGCCCAGGT CTTCAGGCTG CAACAGGCCC ATGGTGGAGA GACCTCCATG AGGAGAGTTT 
     4021 TCAACGTCCT TTATCCCATG CCTGCCGACC AGCGGAGACA TGTCAGTATC AACTCTGCCC 
     4081 GTTGGCTGCC TGAGCCAGGG CTTGGCTTGG CCTATGGTAC TAACAAAGGA GACCTGGTGA 
     4141 TCTGCCGACC AGAGGCCTTA AACTCTGGTG TTGAGTACTA CTGGGACCAG CTGAACGAGA 
     4201 CGGTCTTCAC TGTCCATTCC AACAGCAGGA GCAGCGAGCG GCCTGGAACC AGCAGAGCCA 
     4261 CATGGAGGAC AGACAGAGAC ATGGGGCTGA TGAATGCCAT TGGGCTTCAG CCCCGGAACC 
     4321 CTGCCACCTC AGTGACATCT CAGGGCACCC AGACTCTGGC CCTTCAGCTG CAGAATGCCG 
     4381 AAACACAGAC TGAGAGGGAG GTGCCGGAGC CAGGGACAGC CGCCTCAGGT CCTGGTGAAG 
     4441 GTGAGGGTTC AGAGTATGGT GCCAGTGGAG AAGATGCGCT CAGCAGGATC CAGAGGCTGA 
     4501 TGGCGGAGGG CGGCATGACA GCCGTGGTGC AGCGGGAGCA GAGCACCACC ATGGCCTCCA 
     4561 TGGGCGGCTT CGGCAACAAC ATCATCGTCA GCCACCGCAT TCACCGCAGC TCTCAGACGG 
     4621 GCACTGAACC TGGTGCCGCC CACACCTCCT CACCCCAGCC CTCCACCTCT CGGGGACTGC 
     4681 TCCCAGAGGC CGGGCAACTG GCAGAGCGAG GCCTAAGCCC CCGGACAGCT TCCTGGGACC 
     4741 AGCCTGGTAC CCCTGGGCGG GAGCCAACCC AGCCAACCCT GCCCTCTTCC TCCCCTGTCC 
     4801 CCATTCCTGT TTCCCTTCCC AGCGCTGAGG GACCAACCCT CCACTGCGAG TTGACCAATA 
     4861 ACAACCACCT TCTGGATGGT GGCAGCAGCA GGGGGGACGC TGCAGGCCCT AGGGGAGAAC 
     4921 CACGGAACAG GACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     4981 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     5041 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     5101 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     5161 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     5221 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     5281 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     5341 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     5401 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     5461 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     5521 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     5581 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5641 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5701 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     5761 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     5821 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     5881 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     5941 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     6001 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     6061 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     6121 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     6181 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     6241 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     6301 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     6361 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     6421 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     6481 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     6541 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     6601 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6661 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6721 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6781 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     6841 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     6901 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     6961 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     7021 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     7081 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     7141 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     7201 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     7261 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     7321 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     7381 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     7441 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     7501 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     7561 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     7621 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7681 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7741 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7801 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     7861 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     7921 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     7981 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     8041 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     8101 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     8161 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     8221 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     8281 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     8341 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     8401 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     8461 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     8521 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     8581 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     8641 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     8701 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     8761 TAACAAAAAT TTAACGCGAA TTTT
//