LOCUS       RC235243                8634 bp    DNA     circular  SYN 
DEFINITION  PPFIA2 (Myc-DDK tagged) - Homo sapiens protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 2 (PPFIA2), transcript variant 5
ACCESSION   NM_001220476
VERSION     NM_001220476.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4791..4882 
                     /label="Myc-DDK"  
     misc_feature    4912..5497 
                     /label="PolyA signal"  
     misc_feature    4969..4988 
                     /label="Restriction Enzyme"  
     misc_feature    5773..6445 
                     /label="ColE1"  
     misc_feature    6875..7669 
                     /label="Kan/Neo"  
     misc_feature    7704..8006 
                     /label="SV40 ori"  
     misc_feature    8092..8569 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4773..4779 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4773 
                     /label="PPFIA2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATGTGTGAA GTGATGCCCA CGATTAATGA GGACACCCCA ATGAGCCAAA 
     1081 GGGGGTCCCA AAGCAGTGGC TCGGACTCAG ACTCCCATTT TGAGCAGCTG ATGGTGAATA 
     1141 TGCTAGATGA AAGGGATCGT CTTCTAGACA CCCTTCGGGA GACCCAGGAA AGCCTCTCAC 
     1201 TTGCCCAGCA AAGACTTCAG GATGTCATCT ATGACCGAGA CTCACTCCAG AGACAGCTCA 
     1261 ATTCAGCCCT GCCACAGGAT ATCGAATCCC TAACAGGAGG GCTGGCTGGT TCTAAGGGGG 
     1321 CTGATCCACC GGAATTTGCT GCACTGACAA AAGAATTAAA TGCCTGCAGG GAACAACTTC 
     1381 TAGAAAAGGA AGAAGAAATC TCTGAACTTA AAGCTGAAAG AAACAACACA AGACTATTAC 
     1441 TGGAGCATTT GGAGTGCCTT GTGTCACGAC ATGAAAGATC ACTAAGAATG ACGGTGGTAA 
     1501 AACGGCAAGC CCAGTCTCCC TCAGGAGTAT CCAGTGAAGT TGAAGTTCTC AAGGCACTGA 
     1561 AATCTTTGTT TGAGCACCAC AAGGCCTTGG ATGAAAAGGT AAGGGAGCGA CTGAGGGTTT 
     1621 CTTTAGAAAG AGTCTCTGCA CTGGAAGAAG AACTAGCTGC TGCTAATCAG GAGATTGTTG 
     1681 CCTTGCGTGA ACAAAATGTT CATATACAAA GAAAAATGGC ATCAAGCGAG GGATCCACAG 
     1741 AGTCAGAACA TCTTGAAGGG ATGGAACCTG GACAGAAAGT CCATGAGAAG CGTTTGTCCA 
     1801 ATGGTTCTAT AGACTCAACC GATGAAACTA GTCAAATAGT TGAACTACAA GAATTGCTTG 
     1861 AAAAGCAAAA CTATGAAATG GCCCAGATGA AAGAACGTTT AGCAGCCCTT TCTTCCCGAG 
     1921 TGGGAGAGGT GGAACAGGAA GCAGAGACAG CAAGAAAGGA TCTCATTAAA ACAGAAGAAA 
     1981 TGAACACCAA GTATCAAAGG GACATTAGGG AGGCCATGGC ACAAAAGGAA GATATGGAAG 
     2041 AAAGAATTAC AACCCTTGAA AAGCGTTACC TCAGTGCTCA GAGAGAATCT ACCTCCATAC 
     2101 ATGACATGAA TGATAAACTA GAAAATGAGT TAGCAAATAA AGAAGCTATC CTGCGGCAGA 
     2161 TGGAAGAGAA AAACAGACAG TTACAAGAAC GTCTTGAGCT AGCTGAACAA AAGTTGCAGC 
     2221 AGACCATGAG AAAGGCTGAA ACCTTGCCTG AAGTAGAGGC TGAACTGGCT CAGAGAATTG 
     2281 CAGCCCTAAC CAAGGCTGAA GAGAGACATG GAAATATTGA AGAACGTATG AGACATTTAG 
     2341 AGGGTCAACT TGAAGAGAAG AATCAAGAAC TTCAAAGAGC TAGGCAAAGA GAGAAAATGA 
     2401 ATGAGGAGCA TAACAAGAGA TTATCGGATA CGGTTGATAG ACTTCTGACT GAATCCAATG 
     2461 AACGCCTACA ACTACACTTA AAGGAAAGAA TGGCTGCTCT AGAAGAAAAG AATGTTTTAA 
     2521 TTCAAGAATC AGAAACTTTC AGAAAGAATC TTGAAGAATC TTTACATGAT AAGGAAAGAT 
     2581 TAGCAGAAGA AATTGAAAAG CTGAGATCTG AACTTGACCA ATTGAAAATG AGAACTGGCT 
     2641 CTTTAATTGA ACCCACAATA CCAAGAACTC ATCTAGACAC CTCAGCTGAG TTGCGGTACT 
     2701 CAGTGGGATC CCTAGTGGAC AGCCAGTCTG ATTACAGAAC AACTAAAGTA ATAAGAAGAC 
     2761 CAAGGAGAGG CCGCATGGGT GTGCGAAGAG ATGAGCCAAA GGTGAAATCT CTTGGGGATC 
     2821 ACGAGTGGAA TAGAACTCAA CAGATTGGAG TACTAAGCAG CCACCCTTTT GAAAGTGACA 
     2881 CTGAAATGTC TGATATTGAT GATGATGACA GAGAAACAAT TTTTAGCTCA ATGGATCTTC 
     2941 TCTCTCCAAG TGGTCATTCC GATGCCCAGA CGCTAGCCAT GATGCTTCAG GAACAATTGG 
     3001 ATGCCATCAA CAAAGAAATC AGGCTAATTC AGGAAGAAAA AGAATCTACA GAGTTGCGTG 
     3061 CTGAAGAAAT TGAAAATAGA GTGGCTAGTG TGAGCCTCGA AGGCCTGAAT TTGGCAAGGG 
     3121 TCCACCCAGG TACCTCCATT ACTGCCTCTG TTACAGCTTC ATCGCTGGCC AGTTCATCTC 
     3181 CCCCCAGTGG ACACTCAACT CCAAAGCTCA CCCCTCGAAG CCCTGCCAGG GAAATGGATC 
     3241 GGATGGGAGT CATGACACTG CCAAGTGATC TGAGGAAACA TCGGAGAAAG ATTGCAGTTG 
     3301 TGGAAGAAGA TGGTCGAGAG GACAAAGCAA CAATTAAATG TGAAACTTCT CCTCCTCCTA 
     3361 CCCCTAGAGC CCTCAGAATG ACTCACACTC TCCCTTCTTC CTACCACAAT GATGCTCGAA 
     3421 GTAGTTTATC TGTCTCTCTT GAGCCAGAAA GCCTCGGGCT TGGTAGTGCC AACAGCAGCC 
     3481 AAGACTCTCT TCACAAAGCC CCCAAGAAGA AAGGAATCAA GTCTTCAATA GGACGTTTGT 
     3541 TTGGTAAAAA AGAAAAAGCT CGACTTGGGC AGCTCCGAGG CTTTATGGAG ACTGAAGCTG 
     3601 CAGCTCAGGA GTCCCTGGGG TTAGGCAAAC TCGGAACTCA AGCTGAGAAG GATCGAAGAC 
     3661 TAAAGAAAAA GCATGAACTT CTTGAAGAAG CTCGGAGAAA GGGATTACCT TTTGCCCAGT 
     3721 GGGATGGGCC AACTGTGGTC GCATGGCTAG AGCTTTGGTT GGGAATGCCT GCGTGGTACG 
     3781 TGGCAGCCTG CCGAGCCAAC GTGAAGAGTG GTGCCATCAT GTCTGCTTTA TCTGACACTG 
     3841 AGATCCAGAG AGAAATTGGA ATCAGCAATC CACTGCATCG CTTAAAACTT CGATTAGCAA 
     3901 TCCAGGAGAT GGTTTCCCTA ACAAGTCCTT CAGCTCCTCC AACATCTCGA ACTCCTTCAG 
     3961 GCAACGTTTG GGTGACTCAT GAAGAAATGG AAAATCTTGC AGCTCCAGCA AAAACGAAAG 
     4021 AATCTGAGGA AGGAAGCTGG GCCCAGACCC TGGCTTATGG AGATATGAAT CATGAGTGGA 
     4081 TTGGAAATGA ATGGCTTCCC AGCTTGGGGT TACCTCAGTA CAGAAGTTAC TTTATGGAAT 
     4141 GCTTGGTAGA TGCAAGAATG TTAGATCACC TAACAAAAAA AGATCTCCGT GTCCATTTAA 
     4201 AAATGGTGGA TAGTTTCCAT CGAACAAGTT TACAATATGG AATTATGTGC TTAAAGAGGT 
     4261 TGAATTATGA CAGAAAAGAA CTAGAAAGAA GACGGGAAGC AAGCCAACAT GAAATAAAAG 
     4321 ACGTGTTGGT GTGGAGCAAT GACCGAGTTA TTCGCTGGAT ACAAGCAATT GGACTTCGAG 
     4381 AATATGCAAA TAATATACTT GAGAGCGGTG TGCATGGCTC ACTTATAGCC CTGGATGAAA 
     4441 ACTTTGACTA CAGCAGCTTA GCTTTATTAT TACAGATTCC AACACAGAAC ACCCAGGCAA 
     4501 GGCAGATTCT TGAAAGAGAA TACAATAACC TCTTGGCCCT GGGAACTGAA AGGCGACTGG 
     4561 ATGAAAGTGA TGACAAGAAC TTCAGACGTG GATCAACCTG GAGAAGGCAG TTTCCTCCTC 
     4621 GTGAAGTACA TGGAATCAGC ATGATGCCTG GGTCCTCAGA AACATTACCA GCTGGATTTA 
     4681 GGTTAACCAC AACCTCTGGG CAGTCAAGAA AAATGACAAC AGATGTTGCT TCATCAAGAC 
     4741 TGCAGAGGTT AGACAACTCC ACTGTTCGCA CATACTCATG TACGCGTACG CGGCCGCTCG 
     4801 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4861 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4921 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4981 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     5041 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     5101 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     5161 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     5221 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5281 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5341 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5401 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5461 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5521 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5581 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5641 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5701 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5761 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5821 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5881 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5941 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     6001 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     6061 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     6121 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     6181 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     6241 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6301 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6361 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6421 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6481 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6541 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6601 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6661 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6721 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6781 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6841 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6901 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6961 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     7021 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     7081 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     7141 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     7201 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     7261 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7321 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7381 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7441 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7501 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7561 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7621 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7681 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7741 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7801 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7861 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7921 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7981 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     8041 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     8101 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     8161 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     8221 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     8281 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     8341 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8401 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8461 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8521 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8581 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//