LOCUS       RC235235                8598 bp    DNA     circular  SYN 
DEFINITION  MAP4K4 (Myc-DDK tagged) - Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant 4
ACCESSION   NM_001242559
VERSION     NM_001242559.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4755..4846 
                     /label="Myc-DDK"  
     misc_feature    4876..5461 
                     /label="PolyA signal"  
     misc_feature    4933..4952 
                     /label="Restriction Enzyme"  
     misc_feature    5737..6409 
                     /label="ColE1"  
     misc_feature    6839..7633 
                     /label="Kan/Neo"  
     misc_feature    7668..7970 
                     /label="SV40 ori"  
     misc_feature    8056..8533 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4737..4743 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4737 
                     /label="MAP4K4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGAACGAC TCCCCTGCAA AAAGTCTGGT GGACATCGAC CTCTCCTCCC 
     1081 TGCGGGATCC TGCTGGGATT TTTGAGCTGG TGGAAGTGGT TGGAAATGGC ACCTATGGAC 
     1141 AAGTCTATAA GGGTCGACAT GTTAAAACGG GTCAGTTGGC AGCCATCAAA GTTATGGATG 
     1201 TCACTGAGGA TGAAGAGGAA GAAATCAAAC TGGAGATAAA TATGCTAAAG AAATACTCTC 
     1261 ATCACAGAAA CATTGCAACA TATTATGGTG CTTTCATCAA AAAGAGCCCT CCAGGACATG 
     1321 ATGACCAACT CTGGCTTGTT ATGGAGTTCT GTGGGGCTGG GTCCATTACA GACCTTGTGA 
     1381 AGAACACCAA AGGGAACACA CTCAAAGAAG ACTGGATCGC TTACATCTCC AGAGAAATCC 
     1441 TGAGGGGACT GGCACATCTT CACATTCATC ATGTGATTCA CCGGGATATC AAGGGCCAGA 
     1501 ATGTGTTGCT GACTGAGAAT GCAGAGGTGA AACTTGTTGA CTTTGGTGTG AGTGCTCAGC 
     1561 TGGACAGGAC TGTGGGGCGG AGAAATACGT TCATAGGCAC TCCCTACTGG ATGGCTCCTG 
     1621 AGGTCATCGC CTGTGATGAG AACCCAGATG CCACCTATGA TTACAGAAGT GATCTTTGGT 
     1681 CTTGTGGCAT TACAGCCATT GAGATGGCAG AAGGTGCTCC CCCTCTCTGT GACATGCATC 
     1741 CAATGAGAGC ACTGTTTCTC ATTCCCAGAA ACCCTCCTCC CCGGCTGAAG TCAAAAAAAT 
     1801 GGTCGAAGAA GTTTTTTAGT TTTATAGAAG GGTGCCTGGT GAAGAATTAC ATGCAGCGGC 
     1861 CCTCTACAGA GCAGCTTTTG AAACATCCTT TTATAAGGGA TCAGCCAAAT GAAAGGCAAG 
     1921 TTAGAATCCA GCTTAAGGAT CATATAGATC GTACCAGGAA GAAGAGAGGC GAGAAAGATG 
     1981 AAACTGAGTA TGAGTACAGT GGGAGTGAGG AAGAAGAGGA GGAAGTGCCT GAACAGGAAG 
     2041 GAGAGCCAAG TTCCATTGTG AACGTGCCTG GTGAGTCTAC TCTTCGCCGA GATTTCCTGA 
     2101 GACTGCAGCA GGAGAACAAG GAACGTTCCG AGGCTCTTCG GAGACAACAG TTACTACAGG 
     2161 AGCAACAGCT CCGGGAGCAG GAAGAATATA AAAGGCAACT GCTGGCAGAG AGACAGAAGC 
     2221 GGATTGAGCA GCAGAAAGAA CAGAGGCGAC GGCTAGAAGA GCAACAAAGG AGAGAGCGGG 
     2281 AAGCTAGAAG GCAGCAGGAA CGTGAACAGC GAAGGAGAGA ACAAGAAGAA AAGAGGCGTC 
     2341 TAGAGGAGTT GGAGAGAAGG CGCAAAGAAG AAGAGGAGAG GAGACGGGCA GAAGAAGAAA 
     2401 AGAGGAGAGT TGAAAGAGAA CAGGAGTATA TCAGGCGACA GCTAGAAGAG GAGCAGCGGC 
     2461 ACTTGGAAGT CCTTCAGCAG CAGCTGCTCC AGGAGCAGGC CATGTTACTG GAGTGCCGAT 
     2521 GGCGGGAGAT GGAGGAGCAC CGGCAGGCAG AGAGGCTCCA GAGGCAGTTG CAACAAGAAC 
     2581 AAGCATATCT CCTGTCTCTA CAGCATGACC ATAGGAGGCC GCACCCGCAG CACTCGCAGC 
     2641 AGCCGCCACC ACCGCAGCAG GAAAGGAGCA AGCCAAGCTT CCATGCTCCC GAGCCCAAAG 
     2701 CCCACTACGA GCCTGCTGAC CGAGCGCGAG AGGTGGAAGA TAGATTTAGG AAAACTAACC 
     2761 ACAGCTCCCC TGAAGCCCAG TCTAAGCAGA CAGGCAGAGT ATTGGAGCCA CCAGTGCCTT 
     2821 CCCGATCAGA GTCTTTTTCC AATGGCAACT CCGAGTCTGT GCATCCCGCC CTGCAGAGAC 
     2881 CAGCGGAGCC ACAGGTTCCT GTGAGAACAA CATCTCGCTC CCCTGTTCTG TCCCGTCGAG 
     2941 ATTCCCCACT GCAGGGCAGT GGGCAGCAGA ATAGCCAGGC AGGACAGAGA AACTCCACCA 
     3001 GTATTGAGCC CAGGCTTCTG TGGGAGAGAG TGGAGAAGCT GGTGCCCAGA CCTGGCAGTG 
     3061 GCAGCTCCTC AGGGTCCAGC AACTCAGGAT CCCAGCCCGG GTCTCACCCT GGGTCTCAGA 
     3121 GTGGCTCCGG GGAACGCTTC AGAGTGAGAT CATCATCCAA GTCTGAAGGC TCTCCATCTC 
     3181 AGCGCCTGGA AAATGCAGTG AAAAAACCTG AAGATAAAAA GGAAGTTTTC AGACCCCTCA 
     3241 AGCCTGCTGA TCTGACCGCA CTGGCCAAAG AGCTTCGAGC AGTGGAAGAT GTACGGCCAC 
     3301 CTCACAAAGT AACGGACTAC TCCTCATCCA GTGAGGAGTC GGGGACGACG GATGAGGAGG 
     3361 ACGACGATGT GGAGCAGGAA GGGGCTGACG AGTCCACCTC AGGACCAGAG GACACCAGAG 
     3421 CAGCGTCATC TCTGAATTTG AGCAATGGTG AAACGGAATC TGTGAAAACC ATGATTGTCC 
     3481 ATGATGATGT AGAAAGTGAG CCGGCCATGA CCCCATCCAA GGAGGGCACT CTAATCGTCC 
     3541 GCCAGACTCA GTCCGCTAGT AGCACACTCC AGAAACACAA ATCTTCCTCC TCCTTTACAC 
     3601 CTTTTATAGA CCCCAGATTA CTACAGATTT CTCCATCTAG CGGAACAACA GTGACATCTG 
     3661 TGGTGGGATT TTCCTGTGAT GGGATGAGAC CAGAAGCCAT AAGGCAAGAT CCTACCCGGA 
     3721 AAGGCTCAGT GGTCAATGTG AATCCTACCA ACACTAGGCC ACAGAGTGAC ACCCCGGAGA 
     3781 TTCGTAAATA CAAGAAGAGG TTTAACTCTG AGATTCTGTG TGCTGCCTTA TGGGGAGTGA 
     3841 ATTTGCTAGT GGGTACAGAG AGTGGCCTGA TGCTGCTGGA CAGAAGTGGC CAAGGGAAGG 
     3901 TCTATCCTCT TATCAACCGA AGACGATTTC AACAAATGGA CGTACTTGAG GGCTTGAATG 
     3961 TCTTGGTGAC AATATCTGGC AAAAAGGATA AGTTACGTGT CTACTATTTG TCCTGGTTAA 
     4021 GAAATAAAAT ACTTCACAAT GATCCAGAAG TTGAGAAGAA GCAGGGATGG ACAACCGTAG 
     4081 GGGATTTGGA AGGATGTGTA CATTATAAAG TTGTAAAATA TGAAAGAATC AAATTTCTGG 
     4141 TGATTGCTTT GAAGAGTTCT GTGGAAGTCT ATGCGTGGGC ACCAAAGCCA TATCACAAAT 
     4201 TTATGGCCTT TAAGTCATTT GGAGAATTGG TACATAAGCC ATTACTGGTG GATCTCACTG 
     4261 TTGAGGAAGG CCAGAGGTTG AAAGTGATCT ATGGATCCTG TGCTGGATTC CATGCTGTTG 
     4321 ATGTGGATTC AGGATCAGTC TATGACATTT ATCTACCAAC ACATATCCAG TGTAGCATCA 
     4381 AACCCCATGC AATCATCATC CTCCCCAATA CAGATGGAAT GGAGCTTCTG GTGTGCTATG 
     4441 AAGATGAGGG GGTTTATGTA AACACATATG GAAGGATCAC CAAGGATGTA GTTCTACAGT 
     4501 GGGGAGAGAT GCCTACATCA GTAGCATATA TTCGATCCAA TCAGACAATG GGCTGGGGAG 
     4561 AGAAGGCCAT AGAGATCCGA TCTGTGGAAA CTGGTCACTT GGATGGTGTG TTCATGCACA 
     4621 AAAGGGCTCA AAGACTAAAA TTCTTGTGTG AACGCAATGA CAAGGTGTTC TTTGCCTCTG 
     4681 TTCGGTCTGG TGGCAGCAGT CAGGTTTATT TCATGACCTT AGGCAGGACT TCTCTTCTGA 
     4741 GCTGGACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     4801 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     4861 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4921 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     4981 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     5041 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     5101 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     5161 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     5221 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     5281 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     5341 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     5401 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     5461 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     5521 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     5581 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     5641 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     5701 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     5761 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     5821 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     5881 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     5941 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     6001 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     6061 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     6121 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     6181 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     6241 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     6301 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     6361 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     6421 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     6481 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     6541 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     6601 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     6661 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     6721 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     6781 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     6841 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     6901 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     6961 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     7021 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     7081 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     7141 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     7201 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     7261 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     7321 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     7381 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     7441 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     7501 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     7561 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     7621 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     7681 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     7741 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     7801 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     7861 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7921 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7981 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     8041 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     8101 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     8161 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     8221 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     8281 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     8341 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     8401 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     8461 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     8521 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     8581 AAATTTAACG CGAATTTT
//