LOCUS       RC235233                8589 bp    DNA     circular  SYN 
DEFINITION  PPFIA2 (Myc-DDK tagged) - Homo sapiens protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 2 (PPFIA2), transcript variant 4
ACCESSION   NM_001220475
VERSION     NM_001220475.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4746..4837 
                     /label="Myc-DDK"  
     misc_feature    4867..5452 
                     /label="PolyA signal"  
     misc_feature    4924..4943 
                     /label="Restriction Enzyme"  
     misc_feature    5728..6400 
                     /label="ColE1"  
     misc_feature    6830..7624 
                     /label="Kan/Neo"  
     misc_feature    7659..7961 
                     /label="SV40 ori"  
     misc_feature    8047..8524 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4728..4734 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4728 
                     /label="PPFIA2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATGTGTGAA GTGATGCCCA CGATTAATGA GGACACCCCA ATGAGCCAAA 
     1081 GGGGGTCCCA AAGCAGTGGC TCGGACTCAG ACTCCCATTT TGAGCAGCTG ATGGTGAATA 
     1141 TGCTAGATGA AAGGGATCGT CTTCTAGACA CCCTTCGGGA GACCCAGGAA AGCCTCTCAC 
     1201 TTGCCCAGCA AAGACTTCAG GATGTCATCT ATGACCGAGA CTCACTCCAG AGACAGCTCA 
     1261 ATTCAGCCCT GCCACAGGAT ATCGAATCCC TAACAGGAGG GCTGGCTGGT TCTAAGGGGG 
     1321 CTGATCCACC GGAATTTGCT GCACTGACAA AAGAATTAAA TGCCTGCAGG GAACAACTTC 
     1381 TAGAAAAGGA AGAAGAAATC TCTGAACTTA AAGCTGAAAG AAACAACACA AGACTATTAC 
     1441 TGGAGCATTT GGAGTGCCTT GTGTCACGAC ATGAAAGATC ACTAAGAATG ACGGTGGTAA 
     1501 AACGGCAAGC CCAGTCTCCC TCAGGAGTAT CCAGTGAAGT TGAAGTTCTC AAGGCACTGA 
     1561 AATCTTTGTT TGAGCACCAC AAGGCCTTGG ATGAAAAGGT AAGGGAGCGA CTGAGGGTTT 
     1621 CTTTAGAAAG AGTCTCTGCA CTGGAAGAAG AACTAGCTGC TGCTAATCAG GAGATTGTTG 
     1681 CCTTGCGTGA ACAAAATGTT CATATACAAA GAAAAATGGC ATCAAGCGAG GGATCCACAG 
     1741 AGTCAGAACA TCTTGAAGGG ATGGAACCTG GACAGAAAGT CCATGAGAAG CGTTTGTCCA 
     1801 ATGGTTCTAT AGACTCAACC GATGAAACTA GTCAAATAGT TGAACTACAA GAATTGCTTG 
     1861 AAAAGCAAAA CTATGAAATG GCCCAGATGA AAGAACGTTT AGCAGCCCTT TCTTCCCGAG 
     1921 TGGGAGAGGT GGAACAGGAA GCAGAGACAG CAAGAAAGGA TCTCATTAAA ACAGAAGAAA 
     1981 TGAACACCAA GTATCAAAGG GACATTAGGG AGGCCATGGC ACAAAAGGAA GATATGGAAG 
     2041 AAAGAATTAC AACCCTTGAA AAGCGTTACC TCAGTGCTCA GAGAGAATCT ACCTCCATAC 
     2101 ATGACATGAA TGATAAACTA GAAAATGAGT TAGCAAATAA AGAAGCTATC CTGCGGCAGA 
     2161 TGGAAGAGAA AAACAGACAG TTACAAGAAC GTCTTGAGCT AGCTGAACAA AAGTTGCAGC 
     2221 AGACCATGAG AAAGGCTGAA ACCTTGCCTG AAGTAGAGGC TGAACTGGCT CAGAGAATTG 
     2281 CAGCCCTAAC CAAGGCTGAA GAGAGACATG GAAATATTGA AGAACGTATG AGACATTTAG 
     2341 AGGGTCAACT TGAAGAGAAG AATCAAGAAC TTCAAAGAGC TAGGCAAAGA GAGAAAATGA 
     2401 ATGAGGAGCA TAACAAGAGA TTATCGGATA CGGTTGATAG ACTTCTGACT GAATCCAATG 
     2461 AACGCCTACA ACTACACTTA AAGGAAAGAA TGGCTGCTCT AGAAGAAAAG AATGTTTTAA 
     2521 TTCAAGAATC AGAAACTTTC AGAAAGAATC TTGAAGAATC TTTACATGAT AAGGAAAGAT 
     2581 TAGCAGAAGA AATTGAAAAG CTGAGATCTG AACTTGACCA ATTGAAAATG AGAACTGGCT 
     2641 CTTTAATTGA ACCCACAATA CCAAGAACTC ATCTAGACAC CTCAGCTGAG TTGCGGTACT 
     2701 CAGTGGGATC CCTAGTGGAC AGCCAGTCTG ATTACAGAAC AACTAAAGTA ATAAGAAGAC 
     2761 CAAGGAGAGG CCGCATGGGT GTGCGAAGAG ATGAGCCAAA GGTGAAATCT CTTGGGGATC 
     2821 ACGAGTGGAA TAGAACTCAA CAGATTGGAG TACTAAGCAG CCACCCTTTT GAAAGTGACA 
     2881 CTGAAATGTC TGATATTGAT GATGATGACA GAGAAACAAT TTTTAGCTCA ATGGATCTTC 
     2941 TCTCTCCAAG TGGTCATTCC GATGCCCAGA CGCTAGCCAT GATGCTTCAG GAACAATTGG 
     3001 ATGCCATCAA CAAAGAAATC AGGCTAATTC AGGAAGAAAA AGAATCTACA GAGTTGCGTG 
     3061 CTGAAGAAAT TGAAAATAGA GTGGCTAGTG TGAGCCTCGA AGGCCTGAAT TTGGCAAGGG 
     3121 TCCACCCAGG TACCTCCATT ACTGCCTCTG TTACAGCTTC ATCGCTGGCC AGTTCATCTC 
     3181 CCCCCAGTGG ACACTCAACT CCAAAGCTCA CCCCTCGAAG CCCTGCCAGG GAAATGGATC 
     3241 GGATGGGAGT CATGACACTG CCAAGTGATC TGAGGAAACA TCGGAGAAAG ATTGCAGTTG 
     3301 TGGAAGAAGA TGGTCGAGAG GACAAAGCAA CAATTAAATG TGAAACTTCT CCTCCTCCTA 
     3361 CCCCTAGAGC CCTCAGAATG ACTCACACTC TCCCTTCTTC CTACCACAAT GATGCTCGAA 
     3421 GTAGTTTATC TGTCTCTCTT GAGCCAGAAA GCCTCGGGCT TGGTAGTGCC AACAGCAGCC 
     3481 AAGACTCTCT TCACAAAGCC CCCAAGAAGA AAGGAATCAA GTCTTCAATA GGACGTTTGT 
     3541 TTGGTAAAAA AGAAAAAGCT CGACTTGGGC AGCTCCGAGG CTTTATGGAG ACTGAAGCTG 
     3601 CAGCTCAGGA GTCCCTGGGG TTAGGCAAAC TCGGAACTCA AGCTGAGAAG GATCGAAGAC 
     3661 TAAAGAAAAA GCATGAACTT CTTGAAGAAG CTCGGAGAAA GGGATTACCT TTTGCCCAGT 
     3721 GGGATGGGCC AACTGTGGTC GCATGGCTAG AGCTTTGGTT GGGAATGCCT GCGTGGTACG 
     3781 TGGCAGCCTG CCGAGCCAAC GTGAAGAGTG GTGCCATCAT GTCTGCTTTA TCTGACACTG 
     3841 AGATCCAGAG AGAAATTGGA ATCAGCAATC CACTGCATCG CTTAAAACTT CGATTAGCAA 
     3901 TCCAGGAGAT GGTTTCCCTA ACAAGTCCTT CAGCTCCTCC AACATCTCGA ACTAAAGAAT 
     3961 CTGAGGAAGG AAGCTGGGCC CAGTGTCCGG TTTTTCTACA GACCCTGGCT TATGGAGATA 
     4021 TGAATCATGA GTGGATTGGA AATGAATGGC TTCCCAGCTT GGGGTTACCT CAGTACAGAA 
     4081 GTTACTTTAT GGAATGCTTG GTAGATGCAA GAATGTTAGA TCACCTAACA AAAAAAGATC 
     4141 TCCGTGTCCA TTTAAAAATG GTGGATAGTT TCCATCGAAC AAGTTTACAA TATGGAATTA 
     4201 TGTGCTTAAA GAGGTTGAAT TATGACAGAA AAGAACTAGA AAGAAGACGG GAAGCAAGCC 
     4261 AACATGAAAT AAAAGACGTG TTGGTGTGGA GCAATGACCG AGTTATTCGC TGGATACAAG 
     4321 CAATTGGACT TCGAGAATAT GCAAATAATA TACTTGAGAG CGGTGTGCAT GGCTCACTTA 
     4381 TAGCCCTGGA TGAAAACTTT GACTACAGCA GCTTAGCTTT ATTATTACAG ATTCCAACAC 
     4441 AGAACACCCA GGCAAGGCAG ATTCTTGAAA GAGAATACAA TAACCTCTTG GCCCTGGGAA 
     4501 CTGAAAGGCG ACTGGATGAA AGTGATGACA AGAACTTCAG ACGTGGATCA ACCTGGAGAA 
     4561 GGCAGTTTCC TCCTCGTGAA GTACATGGAA TCAGCATGAT GCCTGGGTCC TCAGAAACAT 
     4621 TACCAGCTGG ATTTAGGTTA ACCACAACCT CTGGGCAGTC AAGAAAAATG ACAACAGATG 
     4681 TTGCTTCATC AAGACTGCAG AGGTTAGACA ACTCCACTGT TCGCACATAC TCATGTACGC 
     4741 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4801 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4861 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4921 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4981 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     5041 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     5101 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     5161 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     5221 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5281 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5341 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5401 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5461 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5521 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5581 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5641 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5701 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5761 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5821 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     5881 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     5941 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     6001 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     6061 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     6121 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     6181 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6241 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6301 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6361 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6421 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6481 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6541 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6601 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6661 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6721 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6781 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6841 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     6901 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     6961 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     7021 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     7081 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     7141 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     7201 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7261 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7321 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7381 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7441 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7501 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7561 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7621 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7681 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7741 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7801 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7861 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     7921 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7981 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     8041 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     8101 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     8161 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     8221 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     8281 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8341 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8401 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8461 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8521 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8581 GCGAATTTT
//