LOCUS       RC235232                8586 bp    DNA     circular  SYN 
DEFINITION  MAP4K4 (Myc-DDK tagged) - Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant 5
ACCESSION   NM_001242560
VERSION     NM_001242560.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4743..4834 
                     /label="Myc-DDK"  
     misc_feature    4864..5449 
                     /label="PolyA signal"  
     misc_feature    4921..4940 
                     /label="Restriction Enzyme"  
     misc_feature    5725..6397 
                     /label="ColE1"  
     misc_feature    6827..7621 
                     /label="Kan/Neo"  
     misc_feature    7656..7958 
                     /label="SV40 ori"  
     misc_feature    8044..8521 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4725..4731 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4725 
                     /label="MAP4K4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGAACGAC TCCCCTGCAA AAAGTCTGGT GGACATCGAC CTCTCCTCCC 
     1081 TGCGGGATCC TGCTGGGATT TTTGAGCTGG TGGAAGTGGT TGGAAATGGC ACCTATGGAC 
     1141 AAGTCTATAA GGGTCGACAT GTTAAAACGG GTCAGTTGGC AGCCATCAAA GTTATGGATG 
     1201 TCACTGAGGA TGAAGAGGAA GAAATCAAAC TGGAGATAAA TATGCTAAAG AAATACTCTC 
     1261 ATCACAGAAA CATTGCAACA TATTATGGTG CTTTCATCAA AAAGAGCCCT CCAGGACATG 
     1321 ATGACCAACT CTGGCTTGTT ATGGAGTTCT GTGGGGCTGG GTCCATTACA GACCTTGTGA 
     1381 AGAACACCAA AGGGAACACA CTCAAAGAAG ACTGGATCGC TTACATCTCC AGAGAAATCC 
     1441 TGAGGGGACT GGCACATCTT CACATTCATC ATGTGATTCA CCGGGATATC AAGGGCCAGA 
     1501 ATGTGTTGCT GACTGAGAAT GCAGAGGTGA AACTTGTTGA CTTTGGTGTG AGTGCTCAGC 
     1561 TGGACAGGAC TGTGGGGCGG AGAAATACGT TCATAGGCAC TCCCTACTGG ATGGCTCCTG 
     1621 AGGTCATCGC CTGTGATGAG AACCCAGATG CCACCTATGA TTACAGAAGT GATCTTTGGT 
     1681 CTTGTGGCAT TACAGCCATT GAGATGGCAG AAGGTGCTCC CCCTCTCTGT GACATGCATC 
     1741 CAATGAGAGC ACTGTTTCTC ATTCCCAGAA ACCCTCCTCC CCGGCTGAAG TCAAAAAAAT 
     1801 GGTCGAAGAA GTTTTTTAGT TTTATAGAAG GGTGCCTGGT GAAGAATTAC ATGCAGCGGC 
     1861 CCTCTACAGA GCAGCTTTTG AAACATCCTT TTATAAGGGA TCAGCCAAAT GAAAGGCAAG 
     1921 TTAGAATCCA GCTTAAGGAT CATATAGATC GTACCAGGAA GAAGAGAGGC GAGAAAGATG 
     1981 AAACTGAGTA TGAGTACAGT GGGAGTGAGG AAGAAGAGGA GGAAGTGCCT GAACAGGAAG 
     2041 GAGAGCCAAG TTCCATTGTG AACGTGCCTG GTGAGTCTAC TCTTCGCCGA GATTTCCTGA 
     2101 GACTGCAGCA GGAGAACAAG GAACGTTCCG AGGCTCTTCG GAGACAACAG TTACTACAGG 
     2161 AGCAACAGCT CCGGGAGCAG GAAGAATATA AAAGGCAACT GCTGGCAGAG AGACAGAAGC 
     2221 GGATTGAGCA GCAGAAAGAA CAGAGGCGAC GGCTAGAAGA GCAACAAAGG AGAGAGCGGG 
     2281 AAGCTAGAAG GCAGCAGGAA CGTGAACAGC GAAGGAGAGA ACAAGAAGAA AAGAGGCGTC 
     2341 TAGAGGAGTT GGAGAGAAGG CGCAAAGAAG AAGAGGAGAG GAGACGGGCA GAAGAAGAAA 
     2401 AGAGGAGAGT TGAAAGAGAA CAGGAGTATA TCAGGCGACA GCTAGAAGAG GAGCAGCGGC 
     2461 ACTTGGAAGT CCTTCAGCAG CAGCTGCTCC AGGAGCAGGC CATGTTACTG CATGACCATA 
     2521 GGAGGCCGCA CCCGCAGCAC TCGCAGCAGC CGCCACCACC GCAGCAGGAA AGGAGCAAGC 
     2581 CAAGCTTCCA TGCTCCCGAG CCCAAAGCCC ACTACGAGCC TGCTGACCGA GCGCGAGAGG 
     2641 TACAGTGGTC CCACCTGGCA TCTCTCAAGA ACAATGTTTC CCCTGTCTCG CGATCCCATT 
     2701 CCTTCAGTGA CCCTTCTCCC AAATTTGCAC ACCACCATCT TCGTTCTCAG GACCCATGTC 
     2761 CACCTTCCCG CAGTGAGGTG CTCAGTCAGA GCTCTGACTC TAAGTCAGAG GCGCCTGACC 
     2821 CTACCCAAAA GGCTTGGTCT AGATCAGACA GTGACGAGGT GCCTCCAAGG GTTCCTGTGA 
     2881 GAACAACATC TCGCTCCCCT GTTCTGTCCC GTCGAGATTC CCCACTGCAG GGCAGTGGGC 
     2941 AGCAGAATAG CCAGGCAGGA CAGAGAAACT CCACCAGCAG TATTGAGCCC AGGCTTCTGT 
     3001 GGGAGAGAGT GGAGAAGCTG GTGCCCAGAC CTGGCAGTGG CAGCTCCTCA GGGTCCAGCA 
     3061 ACTCAGGATC CCAGCCCGGG TCTCACCCTG GGTCTCAGAG TGGCTCCGGG GAACGCTTCA 
     3121 GAGTGAGATC ATCATCCAAG TCTGAAGGCT CTCCATCTCA GCGCCTGGAA AATGCAGTGA 
     3181 AAAAACCTGA AGATAAAAAG GAAGTTTTCA GACCCCTCAA GCCTGCTGGC GAAGTGGATC 
     3241 TGACCGCACT GGCCAAAGAG CTTCGAGCAG TGGAAGATGT ACGGCCACCT CACAAAGTAA 
     3301 CGGACTACTC CTCATCCAGT GAGGAGTCGG GGACGACGGA TGAGGAGGAC GACGATGTGG 
     3361 AGCAGGAAGG GGCTGACGAG TCCACCTCAG GACCAGAGGA CACCAGAGCA GCGTCATCTC 
     3421 TGAATTTGAG CAATGGTGAA ACGGAATCTG TGAAAACCAT GATTGTCCAT GATGATGTAG 
     3481 AAAGTGAGCC GGCCATGACC CCATCCAAGG AGGGCACTCT AATCGTCCGC CAGACTCAGT 
     3541 CCGCTAGTAG CACACTCCAG AAACACAAAT CTTCCTCCTC CTTTACACCT TTTATAGACC 
     3601 CCAGATTACT ACAGATTTCT CCATCTAGCG GAACAACAGT GACATCTGTG GTGGGATTTT 
     3661 CCTGTGATGG GATGAGACCA GAAGCCATAA GGCAAGATCC TACCCGGAAA GGCTCAGTGG 
     3721 TCAATGTGAA TCCTACCAAC ACTAGGCCAC AGAGTGACAC CCCGGAGATT CGTAAATACA 
     3781 AGAAGAGGTT TAACTCTGAG ATTCTGTGTG CTGCCTTATG GGGAGTGAAT TTGCTAGTGG 
     3841 GTACAGAGAG TGGCCTGATG CTGCTGGACA GAAGTGGCCA AGGGAAGGTC TATCCTCTTA 
     3901 TCAACCGAAG ACGATTTCAA CAAATGGACG TACTTGAGGG CTTGAATGTC TTGGTGACAA 
     3961 TATCTGGCAA AAAGGATAAG TTACGTGTCT ACTATTTGTC CTGGTTAAGA AATAAAATAC 
     4021 TTCACAATGA TCCAGAAGTT GAGAAGAAGC AGGGATGGAC AACCGTAGGG GATTTGGAAG 
     4081 GATGTGTACA TTATAAAGTT GTAAAATATG AAAGAATCAA ATTTCTGGTG ATTGCTTTGA 
     4141 AGAGTTCTGT GGAAGTCTAT GCGTGGGCAC CAAAGCCATA TCACAAATTT ATGGCCTTTA 
     4201 AGTCATTTGG AGAATTGGTA CATAAGCCAT TACTGGTGGA TCTCACTGTT GAGGAAGGCC 
     4261 AGAGGTTGAA AGTGATCTAT GGATCCTGTG CTGGATTCCA TGCTGTTGAT GTGGATTCAG 
     4321 GATCAGTCTA TGACATTTAT CTACCAACAC ATATCCAGTG TAGCATCAAA CCCCATGCAA 
     4381 TCATCATCCT CCCCAATACA GATGGAATGG AGCTTCTGGT GTGCTATGAA GATGAGGGGG 
     4441 TTTATGTAAA CACATATGGA AGGATCACCA AGGATGTAGT TCTACAGTGG GGAGAGATGC 
     4501 CTACATCAGT AGCATATATT CGATCCAATC AGACAATGGG CTGGGGAGAG AAGGCCATAG 
     4561 AGATCCGATC TGTGGAAACT GGTCACTTGG ATGGTGTGTT CATGCACAAA AGGGCTCAAA 
     4621 GACTAAAATT CTTGTGTGAA CGCAATGACA AGGTGTTCTT TGCCTCTGTT CGGTCTGGTG 
     4681 GCAGCAGTCA GGTTTATTTC ATGACCTTAG GCAGGACTTC TCTTCTGAGC TGGACGCGTA 
     4741 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     4801 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4861 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4921 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     4981 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     5041 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     5101 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     5161 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     5221 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     5281 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     5341 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     5401 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5461 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     5521 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     5581 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     5641 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     5701 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     5761 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     5821 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     5881 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     5941 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     6001 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     6061 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     6121 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     6181 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     6241 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     6301 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     6361 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     6421 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     6481 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     6541 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     6601 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     6661 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     6721 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     6781 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     6841 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     6901 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     6961 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     7021 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     7081 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     7141 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     7201 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     7261 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     7321 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     7381 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     7441 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     7501 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     7561 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     7621 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     7681 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     7741 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     7801 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     7861 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     7921 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7981 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     8041 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     8101 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     8161 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     8221 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     8281 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     8341 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     8401 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     8461 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     8521 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     8581 AATTTT
//