LOCUS       RC235231                8586 bp    DNA     circular  SYN 
DEFINITION  NOTCH2 (Myc-DDK tagged) - Homo sapiens notch 2 (NOTCH2), transcript variant 2
ACCESSION   NM_001200001
VERSION     NM_001200001.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4743..4834 
                     /label="Myc-DDK"  
     misc_feature    4864..5449 
                     /label="PolyA signal"  
     misc_feature    4921..4940 
                     /label="Restriction Enzyme"  
     misc_feature    5725..6397 
                     /label="ColE1"  
     misc_feature    6827..7621 
                     /label="Kan/Neo"  
     misc_feature    7656..7958 
                     /label="SV40 ori"  
     misc_feature    8044..8521 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4725..4731 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4725 
                     /label="NOTCH2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCCGCCCTG CGCCCCGCTC TGCTGTGGGC GCTGCTGGCG CTCTGGCTGT 
     1081 GCTGCGCGGC CCCCGCGCAT GCATTGCAGT GTCGAGATGG CTATGAACCC TGTGTAAATG 
     1141 AAGGAATGTG TGTTACCTAC CACAATGGCA CAGGATACTG CAAATGTCCA GAAGGCTTCT 
     1201 TGGGGGAATA TTGTCAACAT CGAGACCCCT GTGAGAAGAA CCGCTGCCAG AATGGTGGGA 
     1261 CTTGTGTGGC CCAGGCCATG CTGGGGAAAG CCACGTGCCG ATGTGCCTCA GGGTTTACAG 
     1321 GAGAGGACTG CCAGTACTCA ACATCTCATC CATGCTTTGT GTCTCGACCC TGCCTGAATG 
     1381 GCGGCACATG CCATATGCTC AGCCGGGATA CCTATGAGTG CACCTGTCAA GTCGGGTTTA 
     1441 CAGGTAAGGA GTGCCAATGG ACGGATGCCT GCCTGTCTCA TCCCTGTGCA AATGGAAGTA 
     1501 CCTGTACCAC TGTGGCCAAC CAGTTCTCCT GCAAATGCCT CACAGGCTTC ACAGGGCAGA 
     1561 AATGTGAGAC TGATGTCAAT GAGTGTGACA TTCCAGGACA CTGCCAGCAT GGTGGCACCT 
     1621 GCCTCAACCT GCCTGGTTCC TACCAGTGCC AGTGCCCTCA GGGCTTCACA GGCCAGTACT 
     1681 GTGACAGCCT GTATGTGCCC TGTGCACCCT CACCTTGTGT CAATGGAGGC ACCTGTCGGC 
     1741 AGACTGGTGA CTTCACTTTT GAGTGCAACT GCCTTCCAGG TTTTGAAGGG AGCACCTGTG 
     1801 AGAGGAATAT TGATGACTGC CCTAACCACA GGTGTCAGAA TGGAGGGGTT TGTGTGGATG 
     1861 GGGTCAACAC TTACAACTGC CGCTGTCCCC CACAATGGAC AGGACAGTTC TGCACAGAGG 
     1921 ATGTGGATGA ATGCCTGCTG CAGCCCAATG CCTGTCAAAA TGGGGGCACC TGTGCCAACC 
     1981 GCAATGGAGG CTATGGCTGT GTATGTGTCA ACGGCTGGAG TGGAGATGAC TGCAGTGAGA 
     2041 ACATTGATGA TTGTGCCTTC GCCTCCTGTA CTCCAGGCTC CACCTGCATC GACCGTGTGG 
     2101 CCTCCTTCTC TTGCATGTGC CCAGAGGGGA AGGCAGGTCT CCTGTGTCAT CTGGATGATG 
     2161 CATGCATCAG CAATCCTTGC CACAAGGGGG CACTGTGTGA CACCAACCCC CTAAATGGGC 
     2221 AATATATTTG CACCTGCCCA CAAGGCTACA AAGGGGCTGA CTGCACAGAA GATGTGGATG 
     2281 AATGTGCCAT GGCCAATAGC AATCCTTGTG AGCATGCAGG AAAATGTGTG AACACGGATG 
     2341 GCGCCTTCCA CTGTGAGTGT CTGAAGGGTT ATGCAGGACC TCGTTGTGAG ATGGACATCA 
     2401 ATGAGTGCCA TTCAGACCCC TGCCAGAATG ATGCTACCTG TCTGGATAAG ATTGGAGGCT 
     2461 TCACATGTCT GTGCATGCCA GGTTTCAAAG GTGTGCATTG TGAATTAGAA ATAAATGAAT 
     2521 GTCAGAGCAA CCCTTGTGTG AACAATGGGC AGTGTGTGGA TAAAGTCAAT CGTTTCCAGT 
     2581 GCCTGTGTCC TCCTGGTTTC ACTGGGCCAG TTTGCCAGAT TGATATTGAT GACTGTTCCA 
     2641 GTACTCCGTG TCTGAATGGG GCAAAGTGTA TCGATCACCC GAATGGCTAT GAATGCCAGT 
     2701 GTGCCACAGG TTTCACTGGT GTGTTGTGTG AGGAGAACAT TGACAACTGT GACCCCGATC 
     2761 CTTGCCACCA TGGTCAGTGT CAGGATGGTA TTGATTCCTA CACCTGCATC TGCAATCCCG 
     2821 GGTACATGGG CGCCATCTGC AGTGACCAGA TTGATGAATG TTACAGCAGC CCTTGCCTGA 
     2881 ACGATGGTCG CTGCATTGAC CTGGTCAATG GCTACCAGTG CAACTGCCAG CCAGGCACGT 
     2941 CAGGGGTTAA TTGTGAAATT AATTTTGATG ACTGTGCAAG TAACCCTTGT ATCCATGGAA 
     3001 TCTGTATGGA TGGCATTAAT CGCTACAGTT GTGTCTGCTC ACCAGGATTC ACAGGGCAGA 
     3061 GATGTAACAT TGACATTGAT GAGTGTGCCT CCAATCCCTG TCGCAAGGGT GCAACATGTA 
     3121 TCAACGGTGT GAATGGTTTC CGCTGTATAT GCCCCGAGGG ACCCCATCAC CCCAGCTGCT 
     3181 ACTCACAGGT GAACGAATGC CTGAGCAATC CCTGCATCCA TGGAAACTGT ACTGGAGGTC 
     3241 TCAGTGGATA TAAGTGTCTC TGTGATGCAG GCTGGGTTGG CATCAACTGT GAAGTGGACA 
     3301 AAAATGAATG CCTTTCGAAT CCATGCCAGA ATGGAGGAAC TTGTGACAAT CTGGTGAATG 
     3361 GATACAGGTG TACTTGCAAG AAGGGCTTTA AAGGCTATAA CTGCCAGGTG AATATTGATG 
     3421 AATGTGCCTC AAATCCATGC CTGAACCAAG GAACCTGCTT TGATGACATA AGTGGCTACA 
     3481 CTTGCCACTG TGTGCTGCCA TACACAGGCA AGAATTGTCA GACAGTATTG GCTCCCTGTT 
     3541 CCCCAAACCC TTGTGAGAAT GCTGCTGTTT GCAAAGAGTC ACCAAATTTT GAGAGTTATA 
     3601 CTTGCTTGTG TGCTCCTGGC TGGCAAGGTC AGCGGTGTAC CATTGACATT GACGAGTGTA 
     3661 TCTCCAAGCC CTGCATGAAC CATGGTCTCT GCCATAACAC CCAGGGCAGC TACATGTGTG 
     3721 AATGTCCACC AGGCTTCAGT GGTATGGACT GTGAGGAGGA CATTGATGAC TGCCTTGCCA 
     3781 ATCCTTGCCA GAATGGAGGT TCCTGTATGG ATGGAGTGAA TACTTTCTCC TGCCTCTGCC 
     3841 TTCCGGGTTT CACTGGGGAT AAGTGCCAGA CAGACATGAA TGAGTGTCTG AGTGAACCCT 
     3901 GTAAGAATGG AGGGACCTGC TCTGACTACG TCAACAGTTA CACTTGCAAG TGCCAGGCAG 
     3961 GATTTGATGG AGTCCATTGT GAGAACAACA TCAATGAGTG CACTGAGAGC TCCTGTTTCA 
     4021 ATGGTGGCAC ATGTGTTGAT GGGATTAACT CCTTCTCTTG CTTGTGCCCT GTGGGTTTCA 
     4081 CTGGATCCTT CTGCCTCCAT GAGATCAATG AATGCAGCTC TCATCCATGC CTGAATGAGG 
     4141 GAACGTGTGT TGATGGCCTG GGTACCTACC GCTGCAGCTG CCCCCTGGGC TACACTGGGA 
     4201 AAAACTGTCA GACCCTGGTG AATCTCTGCA GTCGGTCTCC ATGTAAAAAC AAAGGTACTT 
     4261 GCGTTCAGAA AAAAGCAGAG TCCCAGTGCC TATGTCCATC TGGATGGGCT GGTGCCTATT 
     4321 GTGACGTGCC CAATGTCTCT TGTGACATAG CAGCCTCCAG GAGAGGTGTG CTTGTTGAAC 
     4381 ACTTGTGCCA GCACTCAGGT GTCTGCATCA ATGCTGGCAA CACGCATTAC TGTCAGTGCC 
     4441 CCCTGGGCTA TACTGGGAGC TACTGTGAGG AGCAACTCGA TGAGTGTGCG TCCAACCCCT 
     4501 GCCAGCACGG GGCAACATGC AGTGACTTCA TTGGTGGATA CAGATGCGAG TGTGTCCCAG 
     4561 GCTATCAGGG TGTCAACTGT GAGTATGAAG TGGATGAGTG CCAGAATCAG CCCTGCCAGA 
     4621 ATGGAGGCAC CTGTATTGAC CTTGTGAACC ATTTCAAGTG CTCTTGCCCA CCAGGCACTC 
     4681 GGGGTATGAA ATCATCCTTA TCCATTTTCC ATCCAGGGCA TTGTCTTAAG TTAACGCGTA 
     4741 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     4801 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4861 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4921 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     4981 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     5041 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     5101 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     5161 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     5221 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     5281 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     5341 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     5401 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5461 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     5521 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     5581 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     5641 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     5701 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     5761 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     5821 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     5881 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     5941 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     6001 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     6061 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     6121 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     6181 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     6241 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     6301 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     6361 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     6421 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     6481 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     6541 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     6601 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     6661 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     6721 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     6781 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     6841 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     6901 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     6961 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     7021 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     7081 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     7141 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     7201 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     7261 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     7321 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     7381 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     7441 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     7501 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     7561 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     7621 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     7681 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     7741 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     7801 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     7861 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     7921 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7981 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     8041 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     8101 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     8161 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     8221 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     8281 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     8341 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     8401 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     8461 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     8521 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     8581 AATTTT
//