LOCUS       RC235230                8577 bp    DNA     circular  SYN 
DEFINITION  PPFIA2 (Myc-DDK tagged) - Homo sapiens protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 2 (PPFIA2), transcript variant 3
ACCESSION   NM_001220474
VERSION     NM_001220474.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4734..4825 
                     /label="Myc-DDK"  
     misc_feature    4855..5440 
                     /label="PolyA signal"  
     misc_feature    4912..4931 
                     /label="Restriction Enzyme"  
     misc_feature    5716..6388 
                     /label="ColE1"  
     misc_feature    6818..7612 
                     /label="Kan/Neo"  
     misc_feature    7647..7949 
                     /label="SV40 ori"  
     misc_feature    8035..8512 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4716..4722 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4716 
                     /label="PPFIA2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATGTGTGAA GTGATGCCCA CGATTAATGA GGACACCCCA ATGAGCCAAA 
     1081 GGGGGTCCCA AAGCAGTGGC TCGGACTCAG ACTCCCATTT TGAGCAGCTG ATGGTGAATA 
     1141 TGCTAGATGA AAGGGATCGT CTTCTAGACA CCCTTCGGGA GACCCAGGAA AGCCTCTCAC 
     1201 TTGCCCAGCA AAGACTTCAG GATGTCATCT ATGACCGAGA CTCACTCCAG AGACAGCTCA 
     1261 ATTCAGCCCT GCCACAGGAA TTTGCTGCAC TGACAAAAGA ATTAAATGCC TGCAGGGAAC 
     1321 AACTTCTAGA AAAGGAAGAA GAAATCTCTG AACTTAAAGC TGAAAGAAAC AACACAAGAC 
     1381 TATTACTGGA GCATTTGGAG TGCCTTGTGT CACGACATGA AAGATCACTA AGAATGACGG 
     1441 TGGTAAAACG GCAAGCCCAG TCTCCCTCAG GAGTATCCAG TGAAGTTGAA GTTCTCAAGG 
     1501 CACTGAAATC TTTGTTTGAG CACCACAAGG CCTTGGATGA AAAGGTAAGG GAGCGACTGA 
     1561 GGGTTTCTTT AGAAAGAGTC TCTGCACTGG AAGAAGAACT AGCTGCTGCT AATCAGGAGA 
     1621 TTGTTGCCTT GCGTGAACAA AATGTTCATA TACAAAGAAA AATGGCATCA AGCGAGGGAT 
     1681 CCACAGAGTC AGAACATCTT GAAGGGATGG AACCTGGACA GAAAGTCCAT GAGAAGCGTT 
     1741 TGTCCAATGG TTCTATAGAC TCAACCGATG AAACTAGTCA AATAGTTGAA CTACAAGAAT 
     1801 TGCTTGAAAA GCAAAACTAT GAAATGGCCC AGATGAAAGA ACGTTTAGCA GCCCTTTCTT 
     1861 CCCGAGTGGG AGAGGTGGAA CAGGAAGCAG AGACAGCAAG AAAGGATCTC ATTAAAACAG 
     1921 AAGAAATGAA CACCAAGTAT CAAAGGGACA TTAGGGAGGC CATGGCACAA AAGGAAGATA 
     1981 TGGAAGAAAG AATTACAACC CTTGAAAAGC GTTACCTCAG TGCTCAGAGA GAATCTACCT 
     2041 CCATACATGA CATGAATGAT AAACTAGAAA ATGAGTTAGC AAATAAAGAA GCTATCCTGC 
     2101 GGCAGATGGA AGAGAAAAAC AGACAGTTAC AAGAACGTCT TGAGCTAGCT GAACAAAAGT 
     2161 TGCAGCAGAC CATGAGAAAG GCTGAAACCT TGCCTGAAGT AGAGGCTGAA CTGGCTCAGA 
     2221 GAATTGCAGC CCTAACCAAG GCTGAAGAGA GACATGGAAA TATTGAAGAA CGTATGAGAC 
     2281 ATTTAGAGGG TCAACTTGAA GAGAAGAATC AAGAACTTCA AAGAGCTAGG CAAAGAGAGA 
     2341 AAATGAATGA GGAGCATAAC AAGAGATTAT CGGATACGGT TGATAGACTT CTGACTGAAT 
     2401 CCAATGAACG CCTACAACTA CACTTAAAGG AAAGAATGGC TGCTCTAGAA GAAAAGAATG 
     2461 TTTTAATTCA AGAATCAGAA ACTTTCAGAA AGAATCTTGA AGAATCTTTA CATGATAAGG 
     2521 AAAGATTAGC AGAAGAAATT GAAAAGCTGA GATCTGAACT TGACCAATTG AAAATGAGAA 
     2581 CTGGCTCTTT AATTGAACCC ACAATACCAA GAACTCATCT AGACACCTCA GCTGAGTTGC 
     2641 GGTACTCAGT GGGATCCCTA GTGGACAGCC AGTCTGATTA CAGAACAACT AAAGTAATAA 
     2701 GAAGACCAAG GAGAGGCCGC ATGGGTGTGC GAAGAGATGA GCCAAAGGTG AAATCTCTTG 
     2761 GGGATCACGA GTGGAATAGA ACTCAACAGA TTGGAGTACT AAGCAGCCAC CCTTTTGAAA 
     2821 GTGACACTGA AATGTCTGAT ATTGATGATG ATGACAGAGA AACAATTTTT AGCTCAATGG 
     2881 ATCTTCTCTC TCCAAGTGGT CATTCCGATG CCCAGACGCT AGCCATGATG CTTCAGGAAC 
     2941 AATTGGATGC CATCAACAAA GAAATCAGGC TAATTCAGGA AGAAAAAGAA TCTACAGAGT 
     3001 TGCGTGCTGA AGAAATTGAA AATAGAGTGG CTAGTGTGAG CCTCGAAGGC CTGAATTTGG 
     3061 CAAGGGTCCA CCCAGGTACC TCCATTACTG CCTCTGTTAC AGCTTCATCG CTGGCCAGTT 
     3121 CATCTCCCCC CAGTGGACAC TCAACTCCAA AGCTCACCCC TCGAAGCCCT GCCAGGGAAA 
     3181 TGGATCGGAT GGGAGTCATG ACACTGCCAA GTGATCTGAG GAAACATCGG AGAAAGATTG 
     3241 CAGTTGTGGA AGAAGATGGT CGAGAGGACA AAGCAACAAT TAAATGTGAA ACTTCTCCTC 
     3301 CTCCTACCCC TAGAGCCCTC AGAATGACTC ACACTCTCCC TTCTTCCTAC CACAATGATG 
     3361 CTCGAAGTAG TTTATCTGTC TCTCTTGAGC CAGAAAGCCT CGGGCTTGGT AGTGCCAACA 
     3421 GCAGCCAAGA CTCTCTTCAC AAAGCCCCCA AGAAGAAAGG AATCAAGTCT TCAATAGGAC 
     3481 GTTTGTTTGG TAAAAAAGAA AAAGCTCGAC TTGGGCAGCT CCGAGGCTTT ATGGAGACTG 
     3541 AAGCTGCAGC TCAGGAGTCC CTGGGGTTAG GCAAACTCGG AACTCAAGCT GAGAAGGATC 
     3601 GAAGACTAAA GAAAAACACA TCTGGGCATG AACTTCTTGA AGAAGCTCGG AGAAAGGGAT 
     3661 TACCTTTTGC CCAGTGGGAT GGGCCAACTG TGGTCGCATG GCTAGAGCTT TGGTTGGGAA 
     3721 TGCCTGCGTG GTACGTGGCA GCCTGCCGAG CCAACGTGAA GAGTGGTGCC ATCATGTCTG 
     3781 CTTTATCTGA CACTGAGATC CAGAGAGAAA TTGGAATCAG CAATCCACTG CATCGCTTAA 
     3841 AACTTCGATT AGCAATCCAG GAGATGGTTT CCCTAACAAG TCCTTCAGCT CCTCCAACAT 
     3901 CTCGAACTCC TTCAGGCAAC GTTTGGGTGA CTCATGAAGA AATGGAAAAT CTTGCAGCTC 
     3961 CAGCAAAAAC GAAAGAATCT GAGGAAGGAA GCTGGGCCCA GTGTCCGGTT TTTCTACAGA 
     4021 CCCTGGCTTA TGGAGATATG AATCATGAGT GGATTGGAAA TGAATGGCTT CCCAGCTTGG 
     4081 GGTTACCTCA GTACAGAAGT TACTTTATGG AATGCTTGGT AGATGCAAGA ATGTTAGATC 
     4141 ACCTAACAAA AAAAGATCTC CGTGTCCATT TAAAAATGGT GGATAGTTTC CATCGAACAA 
     4201 GTTTACAATA TGGAATTATG TGCTTAAAGA GGTTGAATTA TGACAGAAAA GAACTAGAAA 
     4261 GAAGACGGGA AGCAAGCCAA CATGAAATAA AAGACGTGTT GGTGTGGAGC AATGACCGAG 
     4321 TTATTCGCTG GATACAAGCA ATTGGACTTC GAGAATATGC AAATAATATA CTTGAGAGCG 
     4381 GTGTGCATGG CTCACTTATA GCCCTGGATG AAAACTTTGA CTACAGCAGC TTAGCTTTAT 
     4441 TATTACAGAT TCCAACACAG AACACCCAGG CAAGGCAGAT TCTTGAAAGA GAATACAATA 
     4501 ACCTCTTGGC CCTGGGAACT GAAAGGCGAC TGGATGAAAG TGATGACAAG AACTTCAGAC 
     4561 GTGGATCAAC CTGGAGAAGG CAGTTTCCTC CTCGTGAAGT ACATGGAATC AGCATGATGC 
     4621 CTGGGTCCTC AGAAACATTA CCAGCTGGAT TTAGGTTAAC CACAACCTCT GGGCAGTCAA 
     4681 GAAAAATGAC AACAGATGAT GGCGTCTTTT CAGTCTACTC TACCACGCGT ACGCGGCCGC 
     4741 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     4801 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4861 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4921 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4981 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     5041 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     5101 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     5161 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     5221 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     5281 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     5341 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     5401 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5461 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     5521 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5581 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5641 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     5701 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     5761 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     5821 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     5881 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     5941 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     6001 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     6061 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     6121 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     6181 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     6241 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     6301 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     6361 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     6421 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     6481 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6541 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6601 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6661 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     6721 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     6781 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     6841 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     6901 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     6961 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     7021 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     7081 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     7141 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     7201 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     7261 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     7321 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     7381 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     7441 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     7501 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7561 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7621 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7681 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     7741 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     7801 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7861 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     7921 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     7981 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     8041 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     8101 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     8161 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     8221 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     8281 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     8341 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     8401 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     8461 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     8521 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//