LOCUS       RC235227                8562 bp    DNA     circular  SYN 
DEFINITION  SLC4A2 (Myc-DDK tagged) - Homo sapiens solute carrier family 4 (anion exchanger), member 2 (SLC4A2), transcript variant 4
ACCESSION   NM_001199694
VERSION     NM_001199694.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4719..4810 
                     /label="Myc-DDK"  
     misc_feature    4840..5425 
                     /label="PolyA signal"  
     misc_feature    4897..4916 
                     /label="Restriction Enzyme"  
     misc_feature    5701..6373 
                     /label="ColE1"  
     misc_feature    6803..7597 
                     /label="Kan/Neo"  
     misc_feature    7632..7934 
                     /label="SV40 ori"  
     misc_feature    8020..8497 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4701..4707 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4701 
                     /label="SLC4A2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACTCAGCCA GAGCCAGAGA GCTTGGGCCC TGGGACGCCT GGGTTCCCCG 
     1081 AGCAGGAGGA AGACGAACTT CACCGCACCC TGGGCGTGGA GCGGTTTGAG GAGATCCTAC 
     1141 AGGAGGCCGG GTCTCGTGGA GGGGAGGAGC CAGGCCGCAG CTATGGGGAG GAAGACTTTG 
     1201 AGTACCACCG CCAGTCCTCC CACCACATCC ATCACCCACT GTCCACCCAC CTGCCTCCGG 
     1261 ATGCACGCCG CCGCAAGACA CCCCAGGGCC CAGGACGGAA GCCTCGAAGG CGCCCGGGAG 
     1321 CCTCCCCGAC TGGAGAAACC CCGACCATTG AGGAGGGGGA GGAAGATGAG GATGAGGCCA 
     1381 GCGAGGCTGA GGGGGCCCGG GCTCTCACTC AGCCGTCCCC TGTCTCCACA CCCTCCTCGG 
     1441 TGCAGTTCTT TCTCCAAGAG GATGACAGTG CTGACCGGAA GGCAGAGAGG ACCAGTCCAT 
     1501 CTTCCCCTGC ACCACTGCCC CACCAGGAGG CGACTCCTCG GGCCTCCAAA GGGGCCCAGG 
     1561 CTGGAACCCA GGTGGAGGAG GCGGAGGCGG AGGCGGTGGC GGTGGCCAGT GGCACTGCAG 
     1621 GGGGTGACGA CGGGGGTGCC TCGGGGCGCC CCCTGCCCAA AGCCCAGCCT GGGCACCGCA 
     1681 GCTACAACCT TCAGGAGAGG AGGCGCATCG GGAGCATGAC TGGGGCTGAG CAGGCACTGC 
     1741 TGCCCCGGGT CCCCACGGAT GAGATTGAGG CCCAGACGCT GGCCACGGCC GACCTAGACC 
     1801 TCATGAAGAG TCACCGGTTT GAGGACGTTC CTGGGGTGCG GCGGCACTTG GTGCGGAAGA 
     1861 ATGCCAAAGG TTCCACACAG AGTGGCCGAG AAGGGCGGGA GCCTGGCCCC ACACCTCGGG 
     1921 CCCGACCCCG GGCCCCCCAC AAGCCCCATG AGGTGTTTGT GGAGCTGAAT GAGTTGCTCC 
     1981 TGGACAAAAA CCAGGAGCCC CAGTGGCGGG AGACAGCTCG CTGGATCAAA TTTGAAGAGG 
     2041 ACGTGGAGGA GGAGACTGAG CGCTGGGGGA AGCCCCACGT GGCCTCCCTC TCCTTCCGCA 
     2101 GTCTCCTGGA GCTCCGCAGG ACCCTGGCCC ATGGGGCTGT GCTCTTGGAT CTGGACCAGC 
     2161 AGACCCTGCC CGGAGTGGCC CACCAGGTGG TGGAGCAGAT GGTCATCTCT GACCAGATCA 
     2221 AGGCCGAGGA CAGGGCCAAC GTGCTGCGGG CTCTGCTGTT GAAACACAGC CACCCAAGTG 
     2281 ATGAGAAGGA CTTCTCCTTC CCCCGCAACA TCTCAGCTGG CTCCCTGGGC TCCCTGCTGG 
     2341 GGCATCACCA TGGTCAGGGG GCTGAGAGTG ACCCCCACGT CACCGAGCCT CTCATGGGAG 
     2401 GTGTTCCTGA GACCCGGCTG GAGGTGGAGC GAGAGCGTGA GCTGCCGCCT CCAGCACCAC 
     2461 CAGCTGGCAT CACCCGCTCC AAGTCCAAGC ACGAGCTGAA ACTGCTGGAG AAGATTCCTG 
     2521 AGAATGCCGA GGCCACGGTG GTCCTTGTGG GCTGCGTGGA GTTCCTCTCC CGCCCCACCA 
     2581 TGGCCTTTGT GCGGCTCCGG GAGGCTGTGG AGTTGGACGC AGTGTTGGAG GTGCCGGTGC 
     2641 CTGTGCGTTT CCTCTTCCTG CTGCTGGGCC CGAGTAGTGC CAACATGGAC TACCACGAGA 
     2701 TCGGCCGCTC CATCTCCACC CTCATGTCAG ACAAGCAATT CCACGAGGCA GCCTACCTGG 
     2761 CTGACGAGCG GGAGGACCTG CTGACGGCCA TCAACGCCTT CCTGGACTGC AGCGTGGTGC 
     2821 TGCCGCCTTC AGAAGTGCAG GGCGAGGAGC TGCTGCGCTC TGTGGCCCAC TTCCAGCGCC 
     2881 AGATGCTCAA GAAGCGAGAG GAGCAGGGCC GGCTGCTACC TACAGGGGCT GGGCTGGAGC 
     2941 CCAAATCTGC CCAAGATAAG GCGCTCCTGC AGATGGTAGA GGCGGCAGGG GCAGCTGAAG 
     3001 ATGATCCCCT TCGGCGGACG GGGCGGCCCT TTGGGGGGCT GATCCGAGAT GTGCGGCGCC 
     3061 GCTATCCCCA CTACCTGAGT GACTTCCGAG ATGCACTTGA CCCTCAGTGC CTGGCCGCAG 
     3121 TCATCTTCAT CTACTTTGCC GCCCTGTCTC CTGCCATCAC CTTTGGGGGG CTGCTGGGAG 
     3181 AGAAGACGCA GGACCTGATA GGGGTGTCGG AGCTGATTAT GTCCACAGCG CTCCAGGGCG 
     3241 TGGTCTTCTG CCTGCTGGGT GCCCAGCCCC TGTTGGTGAT CGGCTTCTCA GGGCCCCTGC 
     3301 TGGTCTTTGA GGAGGCCTTC TTCTCGTTCT GTAGCAGCAA CCACCTGGAG TACCTGGTGG 
     3361 GCCGTGTGTG GATCGGCTTC TGGCTGGTGT TCCTGGCCCT GCTCATGGTG GCCCTGGAGG 
     3421 GGAGCTTCCT GGTCCGCTTC GTCTCCCGCT TCACCCAGGA GATCTTCGCC TTCTTGATCT 
     3481 CACTCATCTT CATCTATGAG ACCTTCTACA AGCTGGTGAA GATCTTCCAG GAGCACCCCC 
     3541 TGCATGGCTG CTCAGCCTCC AACAGCTCAG AGGTGGACGG CGGTGAGAAC ATGACATGGG 
     3601 CCGGGGCAAG ACCCACGCTG GGGCCGGGCA ACAGGAGCTT GGCTGGGCAG TCTGGGCAGG 
     3661 GGAAGCCCCG GGGCCAGCCC AACACGGCCC TGCTGTCGCT GGTGCTCATG GCCGGCACCT 
     3721 TCTTCATCGC CTTCTTCCTG CGCAAATTCA AGAACAGCCG GTTCTTTCCT GGCCGGATCC 
     3781 GGCGGGTGAT TGGGGACTTT GGGGTGCCCA TCGCCATCCT CATCATGGTG CTTGTGGATT 
     3841 ACAGTATTGA GGACACCTAT ACCCAGAAGC TGAGCGTTCC CAGTGGATTC TCGGTGACTG 
     3901 CCCCAGAAAA GAGGGGCTGG GTCATCAACC CCCTGGGAGA GAAGAGCCCC TTCCCTGTGT 
     3961 GGATGATGGT TGCCAGCCTG CTGCCCGCCA TCCTGGTCTT CATTCTCATC TTCATGGAGA 
     4021 CACAGATCAC CACGCTCATC ATCTCCAAGA AGGAGCGCAT GCTGCAGAAG GGCTCCGGCT 
     4081 TCCACCTGGA CCTGCTGCTC ATCGTGGCCA TGGGCGGCAT CTGTGCCCTC TTTGGCCTGC 
     4141 CCTGGTTGGC TGCTGCCACT GTCCGCTCTG TCACTCACGC CAACGCGCTC ACTGTCATGA 
     4201 GCAAGGCTGT GGCACCTGGG GACAAGCCCA AGATTCAGGA AGTCAAGGAG CAGCGGGTGA 
     4261 CGGGGCTGCT GGTTGCCCTG CTTGTGGGCC TCTCCATAGT TATCGGGGAT CTGCTCCGGC 
     4321 AGATCCCCCT GGCCGTGCTC TTTGGAATTT TCCTGTACAT GGGAGTCACC TCCCTTAACG 
     4381 GGATCCAGTT CTATGAGCGG CTGCATCTGC TGCTCATGCC GCCCAAACAC CACCCAGATG 
     4441 TCACTTACGT CAAGAAGGTC CGGACCCTCC GTATGCACCT GTTCACGGCC CTGCAGCTGC 
     4501 TCTGCCTGGC CCTGCTCTGG GCCGTCATGT CCACAGCTGC CTCCCTGGCC TTCCCCTTCA 
     4561 TCCTCATCCT CACAGTGCCG CTCCGCATGG TGGTGCTCAC CCGTATCTTC ACCGACCGAG 
     4621 AGATGAAATG TCTGGATGCT AACGAGGCAG AGCCGGTGTT TGATGAGCGG GAGGGTGTGG 
     4681 ACGAGTACAA TGAGATGCCC ATGCCTGTGA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     4741 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     4801 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     4861 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4921 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     4981 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     5041 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     5101 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     5161 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     5221 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     5281 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     5341 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     5401 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     5461 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     5521 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     5581 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     5641 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     5701 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     5761 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     5821 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     5881 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     5941 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     6001 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     6061 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     6121 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     6181 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     6241 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     6301 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     6361 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     6421 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     6481 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     6541 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     6601 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     6661 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     6721 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     6781 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     6841 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     6901 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     6961 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     7021 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     7081 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     7141 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     7201 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     7261 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     7321 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     7381 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     7441 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     7501 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     7561 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     7621 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     7681 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     7741 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     7801 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7861 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7921 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     7981 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     8041 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     8101 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     8161 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     8221 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     8281 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     8341 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     8401 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     8461 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     8521 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//