LOCUS       RC235222                8523 bp    DNA     circular  SYN 
DEFINITION  FLII (Myc-DDK tagged) - Homo sapiens flightless I homolog (Drosophila) (FLII), transcript variant 3
ACCESSION   NM_001256265
VERSION     NM_001256265.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4680..4771 
                     /label="Myc-DDK"  
     misc_feature    4801..5386 
                     /label="PolyA signal"  
     misc_feature    4858..4877 
                     /label="Restriction Enzyme"  
     misc_feature    5662..6334 
                     /label="ColE1"  
     misc_feature    6764..7558 
                     /label="Kan/Neo"  
     misc_feature    7593..7895 
                     /label="SV40 ori"  
     misc_feature    7981..8458 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4662..4668 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4662 
                     /label="FLII"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGGCCACC GGGGTGCTGC CGTTCGTGCG TGGCGTGGAC CTCAGCGGCA 
     1081 ACGACTTCAA GGGCGGCTAC TTCCCTGAGA ATGTCAAGGC CATGACCAGC CTGCGGTGGC 
     1141 TGAAGCTGAA CCGCACTGGC CTCTGCTACC TGCCCGAGGA GCTGGCCGCC CTGCAGAAGC 
     1201 TGGAACACTT GTCTGTGAGC CACAACAACC TGACCACGCT TCATGGGGAG CTGTCCAGCC 
     1261 TGCCATCGCT GCGCGCCATC GTGGCCCGAG CCAACAGTCT GAAGAATTCC GGAGTCCCCG 
     1321 ATGACATCTT CAAGCTAGAT GATCTCTCAG TCCTGGACTT GAGCCACAAC CAGCTGACAG 
     1381 AGTGCCCGCG GGAGCTGGAG AACGCCAAGA ACATGCTGGT GCTGAACCTC AGCCACAACA 
     1441 GGCAGCTCCC AGCGATGACG GCCCTGCAGA CCCTGCACCT GCGGAGCACC CAGCGCACCC 
     1501 AGAGCAACCT GCCCACCAGC CTGGAGGGTC TGAGCAACCT CGCAGACGTG GATCTGTCCT 
     1561 GCAATGACCT GACACGGGTG CCCGAGTGTC TGTACACCCT CCCCAGCCTG CGCCGCCTCA 
     1621 ACCTCAGCAG CAACCAGATC ACGGAGCTGT CCCTGTGCAT AGACCAGTGG GTGCACGTGG 
     1681 AAACTCTGAA CCTGTCCCGA AATCAGCTCA CCTCACTGCC CTCAGCCATT TGCAAGCTGA 
     1741 GCAAGCTGAA GAAGCTGTAC CTGAATTCCA ACAAGCTGGA CTTTGACGGG CTGCCCTCAG 
     1801 GCATTGGCAA GCTCACCAAC CTGGAAGAGT TCATGGCTGC CAACAACAAC CTGGAGCTGG 
     1861 TCCCTGAAAG TCTCTGCAGG TGCCCAAAGC TGAGGAAACT TGTCCTGAAC AAGAACCACC 
     1921 TGGTGACCCT CCCAGAAGCC ATCCATTTCC TGACGGAGAT CGAGGTCCTG GATGTGCGGG 
     1981 AGAACCCCAA CCTGGTCATG CCGCCCAAGC CCGCAGACCG TGCCGCTGAG TGGTACAACA 
     2041 TCGACTTCTC GCTGCAGAAC CAGCTGCGGC TAGCGGGTGC CTCTCCTGCT ACCGTGGCTG 
     2101 CAGCTGCAGC TGGGAGTGGG CCCAAGGACC CTATGGCTCG CAAGATGCGA CTGCGGAGGC 
     2161 GCAAGGATTC AGCCCAGGAT GACCAGGCCA AGCAGGTGCT GAAGGGCATG TCAGATGTTG 
     2221 CCCAGGAGAA GAACAAAAAG CAGGAGGAGA GCGCAGATGC CCGGGCCCCC AGCGGGAAGG 
     2281 TGCGGCGTTG GGACCAGGGC CTGGAGAAGC CCCGCCTTGA CTACTCCGAG TTCTTCACGG 
     2341 AGGACGTGGG CCAGCTGCCC GGACTGACCA TCTGGCAGAT AGAGAACTTC GTGCCTGTGC 
     2401 TGGTGGAGGA AGCCTTCCAC GGCAAGTTCT ACGAGGCTGA CTGCTACATT GTGCTCAAGA 
     2461 CCTTTCTGGA TGACAGCGGC TCCCTCAACT GGGAGATCTA CTACTGGATT GGCGGGGAGG 
     2521 CCACACTCGA CAAGAAAGCT TGCTCTGCCA TCCACGCTGT CAACTTGCGC AACTACCTGG 
     2581 GTGCTGAGTG CCGCACTGTC CGGGAGGAGA TGGGCGATGA GAGCGAGGAG TTCCTGCAGG 
     2641 TGTTTGACAA CGACATCTCC TACATTGAGG GTGGAACAGC CAGTGGCTTC TACACTGTGG 
     2701 AAGACACACA CTATGTCACC AGGATGTATC GTGTGTATGG GAAAAAGAAC ATCAAGTTGG 
     2761 AGCCTGTGCC CCTCAAGGGG ACCTCTCTGG ACCCAAGGTT TGTTTTCCTG CTGGACCGAG 
     2821 GGCTAGACAT CTACGTATGG CGGGGGGCCC AGGCCACACT GAGCAGCACC ACCAAGGCCA 
     2881 GGCTCTTTGC AGAGAAAATT AACAAGAATG AGCGGAAAGG GAAGGCTGAG ATCACACTGC 
     2941 TGGTGCAGGG CCAGGAGCTC CCAGAGTTCT GGGAGGCACT GGGTGGGGAG CCCTCTGAGA 
     3001 TCAAGAAGCA CGTGCCTGAA GACTTCTGGC CGCCGCAGCC CAAGCTGTAC AAGGTGGGCC 
     3061 TGGGCTTGGG CTACCTGGAG CTGCCACAGA TCAACTACAA GCTCTCCGTG GAACATAAGC 
     3121 AGCGTCCCAA GGTGGAGCTG ATGCCAAGAA TGCGGCTGCT GCAGAGTCTG CTGGACACGC 
     3181 GCTGCGTGTA CATTCTGGAC TGTTGGTCCG ACGTGTTCAT CTGGCTCGGC CGCAAGTCCC 
     3241 CGCGCCTGGT GCGCGCTGCC GCCCTCAAGC TGGGTCAGGA GCTGTGCGGG ATGCTGCACC 
     3301 GGCCACGCCA TGCCACGGTC AGCCGCAGCC TCGAGGGCAC CGAGGCGCAG GTGTTCAAGG 
     3361 CCAAGTTCAA GAATTGGGAC GATGTGTTGA CGGTGGACTA CACACGCAAT GCGGAGGCCG 
     3421 TGCTGCAGAG CCCGGGTCTC TCCGGGAAGG TGAAACGCGA CGCCGAGAAG AAAGACCAGA 
     3481 TGAAGGCTGA CCTCACTGCG CTTTTCCTGC CGCGGCAGCC GCCCATGTCG CTGGCCGAGG 
     3541 CGGAGCAGCT GATGGAGGAG TGGAACGAAG ACCTAGACGG CATGGAGGGT TTCGTGCTGG 
     3601 AGGGCAAGAA GTTTGCGCGG CTGCCGGAAG AGGAGTTTGG CCACTTCTAC ACGCAGGACT 
     3661 GCTACGTCTT CCTCTGCAGG TACTGGGTGC CTGTGGAGTA CGAGGAGGAG GAAAAGAAGG 
     3721 AAGACAAGGA GGAGAAGGCC GAGGGCAAAG AAGGCGAGGA AGCAACCGCT GAGGCAGAGG 
     3781 AGAAGCAGCC AGAGGAGGAC TTCCAGTGCA TCGTGTACTT CTGGCAGGGC CGTGAAGCCT 
     3841 CCAATATGGG CTGGCTCACC TTCACCTTCA GCCTGCAAAA GAAGTTCGAG AGCCTCTTCC 
     3901 CTGGGAAGCT GGAGGTGGTA CGCATGACGC AGCAGCAGGA GAACCCCAAG TTCCTGTCCC 
     3961 ATTTCAAGAG GAAGTTCATC ATCCACCGGG GCAAGAGGAA GGCGGTCCAG GGCGCCCAAC 
     4021 AGCCCAGCCT CTACCAGATC CGCACCAACG GCAGCGCCCT CTGCACCCGG TGCATCCAGA 
     4081 TCAACACCGA CTCCAGCCTC CTCAACTCCG AGTTCTGCTT CATCCTCAAG GTTCCCTTTG 
     4141 AGAGTGAGGA CAACCAGGGC ATCGTGTATG CCTGGGTGGG CCGGGCATCA GACCCTGACG 
     4201 AAGCCAAGTT GGCAGAAGAC ATCCTGAACA CCATGTTTGA CACCTCCTAC AGCAAGCAGG 
     4261 TTATCAACGA AGGTGAGGAG CCTGAGAACT TCTTCTGGGT GGGCATTGGG GCACAGAAGC 
     4321 CCTATGATGA CGATGCCGAG TACATGAAAC ACACACGTCT CTTCCGGTGC TCCAACGAGA 
     4381 AGGGCTACTT TGCAGTGACT GAGAAATGCT CCGACTTTTG CCAAGATGAC CTGGCAGATG 
     4441 ATGACATCAT GTTGCTAGAC AATGGCCAAG AGGTCTACAT GTGGGTGGGG ACCCAGACTA 
     4501 GCCAGGTGGA GATCAAGCTG AGCCTGAAGG CCTGCCAGGT ATATATCCAG CACATGCGGT 
     4561 CCAAGGAACA TGAGCGGCCG CGCCGGCTGC GCCTGGTCCG CAAGGGCAAT GAGCAGCACG 
     4621 CCTTTACCCG CTGCTTCCAC GCCTGGAGCG CCTTCTGCAA GGCCCTGGCC ACGCGTACGC 
     4681 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     4741 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4801 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4861 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4921 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4981 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     5041 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     5101 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     5161 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     5221 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     5281 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     5341 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     5401 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     5461 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     5521 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     5581 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     5641 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     5701 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     5761 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     5821 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     5881 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     5941 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     6001 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     6061 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     6121 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     6181 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     6241 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     6301 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     6361 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     6421 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     6481 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     6541 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     6601 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     6661 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     6721 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     6781 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     6841 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     6901 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     6961 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     7021 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     7081 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     7141 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     7201 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     7261 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     7321 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     7381 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     7441 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     7501 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     7561 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     7621 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     7681 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     7741 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     7801 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     7861 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     7921 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     7981 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     8041 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     8101 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     8161 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     8221 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     8281 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     8341 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     8401 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     8461 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     8521 TTT
//