LOCUS       RC235221                8523 bp    DNA     circular  SYN 
DEFINITION  NEK1 (Myc-DDK tagged) - Homo sapiens NIMA-related kinase 1 (NEK1), transcript variant 5
ACCESSION   NM_001199400
VERSION     NM_001199400.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4680..4771 
                     /label="Myc-DDK"  
     misc_feature    4801..5386 
                     /label="PolyA signal"  
     misc_feature    4858..4877 
                     /label="Restriction Enzyme"  
     misc_feature    5662..6334 
                     /label="ColE1"  
     misc_feature    6764..7558 
                     /label="Kan/Neo"  
     misc_feature    7593..7895 
                     /label="SV40 ori"  
     misc_feature    7981..8458 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4662..4668 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4662 
                     /label="NEK1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGAAGTAT GTTAGACTAC AGAAGATTGG AGAAGGTTCA TTTGGAAAAG 
     1081 CCATTCTTGT TAAATCTACA GAAGATGGCA GACAGTATGT TATCAAGGAA ATTAACATCT 
     1141 CAAGAATGTC CAGTAAAGAA AGAGAAGAAT CAAGGAGAGA AGTTGCAGTA TTGGCAAACA 
     1201 TGAAGCATCC AAATATTGTC CAGTATAGAG AATCATTTGA AGAAAATGGC TCTCTCTACA 
     1261 TAGTAATGGA TTACTGTGAG GGAGGGGATC TGTTTAAGCG AATAAATGCT CAGAAAGGCG 
     1321 TTTTGTTTCA AGAGGATCAG ATTTTGGACT GGTTTGTACA GATATGTTTG GCCCTGAAAC 
     1381 ATGTACATGA TAGAAAAATT CTTCATCGAG ACATTAAATC TCAGAACATA TTTTTAACTA 
     1441 AAGATGGAAC AGTACAACTT GGAGATTTTG GAATTGCTAG AGTTCTTAAT AGTACTGTAG 
     1501 AGCTGGCTCG AACTTGCATA GGGACCCCAT ACTACTTGTC ACCTGAAATC TGTGAAAACA 
     1561 AACCTTACAA TAATAAAAGT GACATTTGGG CTCTGGGGTG TGTCCTTTAT GAGCTGTGTA 
     1621 CACTTAAACA TGCTTTTGAA GCTGGCAGTA TGAAAAACCT GGTACTGAAG ATAATATCTG 
     1681 GATCTTTTCC ACCTGTGTCT TTGCATTATT CCTATGATCT CCGCAGTTTG GTGTCTCAGT 
     1741 TATTTAAAAG AAATCCTAGG GATAGACCAT CAGTCAACTC CATATTGGAG AAAGGTTTTA 
     1801 TAGCCAAACG CATTGAAAAG TTTCTCTCTC CTCAGCTTAT TGCAGAAGAA TTTTGTCTAA 
     1861 AAACATTTTC GAAGTTTGGA TCACAGCCTA TACCAGCTAA AAGACCAGCT TCAGGACAAA 
     1921 ACTCGATTTC TGTTATGCCT GCTCAGAAAA TTACAAAGCC TGCCGCTAAA TATGGAATAC 
     1981 CTTTAGCATA TAAGAAATAT GGAGATAAAA AATTACACGA AAAGAAACCA CTGCAAAAAC 
     2041 ATAAACAGGC CCATCAAACT CCAGAGAAGA GAGTGAATAC TGGAGAAGAA AGGAGGAAAA 
     2101 TATCTGAGGA AGCAGCAAGA AAGAGAAGGC TGGAATTTAT TGAAAAAGAA AAGAAACAAA 
     2161 AGGATCAGAT TATTAGTTTA ATGAAGGCTG AACAAATGAA AAGGCAAGAA AAGGAAAGGT 
     2221 TGGAAAGAAT AAATAGGGCC AGGGAACAAG GATGGAGAAA TGTGCTAAGT GCTGGTGGAA 
     2281 GTGGTGAAGT AAAGGCTCCT TTTCTGGGCA GTGGAGGGAC TATAGCTCCA TCATCTTTTT 
     2341 CTTCTCGAGG ACAGTATGAA CATTACCATG CCATTTTTGA CCAAATGCAG CAACAAAGAG 
     2401 CAGAAGATAA TGAAGCTAAA TGGAAAAGAG AAATATATGG TCGAGGTCTT CCAGAAAGGC 
     2461 AAAAAGGGCA GCTAGCTGTA GAAAGAGCTA AACAAGTAGA AGAGTTCCTG CAGCGAAAAC 
     2521 GGGAAGCTAT GCAGAATAAA GCTCGAGCCG AAGGACATAT GGTTTATCTG GCAAGACTGA 
     2581 GGCAAATAAG ACTACAGAAT TTCAATGAGC GCCAACAGAT TAAAGCCAAA CTTCGTGGTG 
     2641 AAAAGAAAGA AGCTAATCAT TCTGAAGGAC AAGAAGGAAG TGAAGAGGCT GACATGAGGC 
     2701 GCAAAAAAAT CGAATCACTG AAGGCCCATG CAAATGCACG TGCTGCTGTA CTAAAAGAAC 
     2761 AACTAGAACG AAAGAGAAAG GAGGCTTATG AGAGAGAAAA AAAAGTGTGG GAAGAGCATT 
     2821 TGGTGGCTAA AGGAGTTAAG AGTTCTGATG TTTCTCCACC TTTGGGACAG CATGAAACAG 
     2881 GTGGCTCTCC ATCAAAGCAA CAGATGAGAT CTGTTATTTC TGTAACTTCA GCTTTGAAAG 
     2941 AAGTTGGCGT GGACAGTAGT TTAACTGATA CCCGGGAAAC TTCAGAAGAG ATGCAAAAGA 
     3001 CCAACAATGC TATTTCAAGT AAGCGAGAAA TACTTCGTAG ATTAAATGAA AATCTTAAAG 
     3061 CTCAAGAAGA TGAAAAAGGA AAGCAGAATC TCTCTGATAC TTTTGAGATA AATGTTCATG 
     3121 AAGATGCCAA AGAGCATGAA AAAGAAAAAT CAGTTTCATC TGATCGCAAG AAGTGGGAGG 
     3181 CAGGAGGTCA ACTTGTGATT CCTCTGGATG AGTTAACACT AGATACATCC TTCTCTACAA 
     3241 CTGAAAGACA TACAGTGGGA GAAGTTATTA AATTAGGTCC TAATGGATCT CCAAGAAGAG 
     3301 CCTGGGGGAA AAGTCCGACA GATTCTGTTC TAAAGATACT TGGAGAAGCT GAACTACAAC 
     3361 TTCAGACAGA ACTATTAGAA AATACAACTA TTAGAAGTGA GATTTCTCCC GAAGGGGAAA 
     3421 AGTACAAACC CTTAATTACT GGAGAAAAAA AAGTACAATG TATTTCACAT GAAATAAACC 
     3481 CATCAGCTAT TGTTGATTCT CCTGTTGAGA CAAAAAGTCC CGAGTTCAGT GAGGCATCTC 
     3541 CACAGATGTC ATTGAAACTG GAAGGAAATT TAGAAGAACC TGATGATTTG GAAACAGAAA 
     3601 TTCTACAAGA GCCAAGTGGA ACAAACAAAG ATGAGAGCTT GCCATGCACT ATTACTGATG 
     3661 TGTGGATTAG TGAGGAAAAA GAAACAAAGG AAACTCAGTC GGCAGATAGG ATCACCATTC 
     3721 AGGAAAATGA AGTTTCTGAA GATGGAGTCT CGAGTACTGT GGACCAACTT AGTGACATTC 
     3781 ATATAGAGCC TGGAACCAAT GATTCTCAGC ACTCTAAATG TGATGTAGAT AAGTCTGTGC 
     3841 AACCGGAACC ATTTTTCCAT AAGGTGGTTC ATTCTGAACA CTTGAACTTA GTCCCTCAAG 
     3901 TTCAATCAGT TCAGTGTTCA CCAGAAGAAT CCTTTGCATT TCGATCTCAC TCGCATTTAC 
     3961 CACCAAAAAA TAAAAACAAG AATTCCTTGC TGATTGGACT TTCAACTGGT CTGTTTGATG 
     4021 CAAACAACCC AAAGATGTTA AGGACATGTT CACTTCCAGA TCTCTCAAAG CTGTTCAGAA 
     4081 CCCTTATGGA TGTTCCCACC GTAGGAGATG TTCGTCAAGA CAATCTTGAA ATAGATGAAA 
     4141 TTGAAGATGA AAACATTAAA GAAGGACCTT CTGATTCTGA AGACATTGTG TTTGAAGAAA 
     4201 CTGACACAGA TTTACAAGAG CTGCAGGCCT CGATGGAACA GTTACTTAGG GAACAACCTG 
     4261 GTGAAGAATA CAGTGAAGAA GAAGAGTCAG TCTTGAAGAA CAGTGATGTG GAGCCAACTG 
     4321 CAAATGGGAC AGATGTGGCA GATGAAGATG ACAATCCCAG CAGTGAAAGT GCCCTGAACG 
     4381 AAGAATGGCA CTCAGATAAC AGTGATGGTG AAATTGCTAG TGAATGTGAA TGCGATAGTG 
     4441 TCTTTAACCA TTTAGAGGAA CTGAGACTTC ATCTGGAGCA GGAAATGGGC TTTGAAAAAT 
     4501 TCTTTGAGGT TTATGAGAAA ATAAAGGCTA TTCATGAAGA TGAAGATGAA AATATTGAAA 
     4561 TTTGTTCAAA AATAGTTCAA AATATTTTGG GAAATGAACA TCAGCATCTT TATGCCAAGA 
     4621 TTCTTCATTT AGTCATGGCA GATGGAGCCT ACCAAGAAGA TAATGATGAA ACGCGTACGC 
     4681 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     4741 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4801 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4861 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4921 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4981 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     5041 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     5101 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     5161 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     5221 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     5281 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     5341 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     5401 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     5461 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     5521 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     5581 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     5641 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     5701 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     5761 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     5821 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     5881 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     5941 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     6001 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     6061 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     6121 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     6181 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     6241 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     6301 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     6361 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     6421 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     6481 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     6541 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     6601 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     6661 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     6721 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     6781 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     6841 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     6901 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     6961 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     7021 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     7081 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     7141 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     7201 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     7261 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     7321 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     7381 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     7441 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     7501 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     7561 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     7621 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     7681 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     7741 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     7801 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     7861 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     7921 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     7981 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     8041 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     8101 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     8161 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     8221 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     8281 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     8341 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     8401 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     8461 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     8521 TTT
//