LOCUS       RC235220                8514 bp    DNA     circular  SYN 
DEFINITION  KCNT1 (Myc-DDK tagged) - Homo sapiens potassium channel, subfamily T, member 1 (KCNT1), transcript variant 2
ACCESSION   NM_001272003
VERSION     NM_001272003.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4671..4762 
                     /label="Myc-DDK"  
     misc_feature    4792..5377 
                     /label="PolyA signal"  
     misc_feature    4849..4868 
                     /label="Restriction Enzyme"  
     misc_feature    5653..6325 
                     /label="ColE1"  
     misc_feature    6755..7549 
                     /label="Kan/Neo"  
     misc_feature    7584..7886 
                     /label="SV40 ori"  
     misc_feature    7972..8449 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4653..4659 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4653 
                     /label="KCNT1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCACTCCCT GACGGGGCGC GGACCCCGGG GGGCGTCTGC CGGGAGGCGC 
     1081 GCGGCGGGGG CTACACCAAC CGGACCTTCG AGTTTGACGA CGGCCAATGC GCCCCCAGGG 
     1141 TCCAGGTGGA GTTCTACGTC AACGAGAACA CCTTCAAGGA GCGGCTCAAG CTGTTCTTCA 
     1201 TCAAAAACCA AAGATCGAGC CTGAGGATCC GGCTGTTCAA CTTCTCCCTG AAGCTGCTCA 
     1261 CCTGCCTGCT CTACATTGTG CGCGTCCTGC TCGATGACCC GGCCCTGGGC ATCGGATGCT 
     1321 GGGGCTGCCC AAAGCAGAAC TACTCCTTCA ATGACTCGTC CTCCGAGATC AACTGGGCTC 
     1381 CTATTCTGTG GGTGGAGAGA AAGATGACAC TGTGGGCGAT CCAGGTCATC GTGGCCATAA 
     1441 TAAGCTTCCT GGAGACGATG CTTCTCATCT ACCTCAGCTA CAAAGGCAAC ATCTGGGAGC 
     1501 AGATCTTCCG CGTGTCCTTC GTCCTGGAGA TGATCAACAC TCTGCCCTTC ATCATCACGA 
     1561 TCTTCTGGCC GCCGCTGCGG AACCTGTTCA TCCCCGTCTT TCTGAACTGC TGGCTGGCCA 
     1621 AGCACGCGCT GGAAAACATG ATTAATGACT TCCACCGTGC CATCCTGCGG ACACAGTCAG 
     1681 CCATGTTCAA CCAGGTCCTC ATCCTCTTCT GCACCCTGCT GTGCCTCGTT TTCACGGGGG 
     1741 GTTGCAGGAC CTGCGGCATC CAGCACCTGG AGCGGGCGGG CGAGAACCTG TCCCTCCTGA 
     1801 CCTCCTTCTA CTTCTGCATC GTCACCTTCT CCACCGTGGG CTACGGTGAC GTCACGCCCA 
     1861 AGATCTGGCC ATCGCAGCTG CTGGTGGTCA TCATGATCTG CGTGGCCCTC GTGGTGCTCC 
     1921 CACTGCAGTT CGAGGAGCTC GTCTACCTCT GGATGGAGCG GCAGAAGTCA GGGGGCAACT 
     1981 ACAGCCGCCA CCGTGCGCAG ACGGAGAAGC ACGTGGTCCT GTGTGTCAGC TCCCTCAAGA 
     2041 TCGACCTTCT CATGGACTTC CTGAACGAGT TCTACGCCCA CCCCCGGCTC CAGGACTATT 
     2101 ACGTGGTCAT CCTGTGCCCC ACGGAGATGG ATGTCCAGGT GCGCAGAGTC CTGCAGATCC 
     2161 CTCTGTGGTC CCAGCGGGTC ATCTACCTCC AGGGCTCTGC ACTCAAAGAC CAGGACCTCA 
     2221 TGCGAGCCAA GATGGACAAT GGGGAGGCCT GCTTCATCCT CAGCAGCAGG AACGAGGTGG 
     2281 ACCGCACGGC TGCAGACCAC CAGACCATCC TGCGCGCCTG GGCCGTGAAG GACTTCGCCC 
     2341 CCAACTGCCC CCTCTACGTC CAGATCCTCA AACCTGAAAA CAAGTTTCAC GTCAAGTTTG 
     2401 CTGACCACGT GGTGTGTGAG GAGGAGTGCA AGTACGCCAT GCTGGCGCTG AACTGCATCT 
     2461 GCCCGGCGAC CTCCACCCTC ATCACCCTGC TGGTGCACAC GTCCCGCGGC CAGGAGGGAC 
     2521 AGGAGTCTCC GGAGCAGTGG CAGCGCATGT ATGGGCGCTG CTCCGGCAAC GAGGTGTACC 
     2581 ACATCCGCAT GGGTGACAGC AAGTTCTTCC GCGAGTACGA GGGCAAGAGC TTCACCTACG 
     2641 CGGCCTTCCA CGCCCACAAG AAGTATGGCG TGTGCCTCAT CGGGCTGAAG CGGGAGGACA 
     2701 ACAAGAGCAT CCTGCTGAAC CCGGGGCCCC GGCACATCCT GGCCGCCTCT GACACCTGCT 
     2761 TCTACATCAA CATCACCAAG GAGGAGAACT CGGCCTTCAT CTTCAAGCAG GAGGAGAAGC 
     2821 GGAAGAAGAG GGCCTTCTCG GGGCAGGGGC TGCACGAGGG TCCGGCCCGC CTGCCCGTGC 
     2881 ACAGCATCAT CGCCTCCATG GGGACAGTGG CCATGGACCT GCAGGGCACA GAGCACCGGC 
     2941 CTACGCAGAG CGGCGGTGGG GGCGGGGGCA GCAAGCTGGC ACTGCCCACG GAGAACGGCT 
     3001 CGGGCAGCCG GCGGCCCAGC ATCGCGCCCG TCCTGGAACT GGCCGACAGC TCAGCCCTGC 
     3061 TGCCCTGCGA CCTGCTGAGC GACCAGTCGG AGGATGAGGT GACGCCGTCG GACGACGAGG 
     3121 GGCTCTCCGT GGTAGAGTAT GTGAAGGGCT ACCCTCCCAA CTCGCCCTAC ATCGGCAGCT 
     3181 CCCCAACCCT GTGCCACCTC CTGCCTGTGA AAGCCCCCTT CTGCTGCCTG CGGCTGGACA 
     3241 AGGGCTGCAA GCACAACAGC TATGAAGACG CCAAGGCCTA CGGGTTCAAG AACAAGCTGA 
     3301 TCATCGTCTC GGCAGAGACG GCCGGCAATG GGCTGTACAA CTTCATCGTG CCACTGCGGG 
     3361 CCTACTACAG ATCCCGCAAG GAGCTGAACC CCATCGTGCT GCTGCTGGAC AACAAGCCCG 
     3421 ACCACCACTT CCTGGAAGCC ATCTGCTGCT TCCCCATGGT CTACTACATG GAGGGCTCTG 
     3481 TGGACAACCT GGACAGCCTG CTGCAGTGTG GCATCATCTA TGCGGACAAC CTGGTGGTGG 
     3541 TGGACAAGGA GAGCACCATG AGCGCCGAGG AGGACTACAT GGCGGACGCC AAGACCATCG 
     3601 TCAACGTGCA GACCATGTTC CGGCTCTTCC CCAGCCTCAG CATCACCACG GAGCTCACCC 
     3661 ACCCTTCCAA CATGCGCTTC ATGCAGTTCC GCGCCAAGGA CAGCTACTCT CTGGCTCTTT 
     3721 CCAAACTAGA AAAGAGGGAG CGAGAGAATG GCTCCAACCT GGCCTTCATG TTCCGCCTGC 
     3781 CGTTCGCCGC CGGCCGCGTC TTCAGCATCA GCATGTTGGA CACACTGCTC TACCAGTCCT 
     3841 TCGTGAAGGA CTACATGATC ACCATCACCC GGCTGCTGCT GGGCCTGGAC ACCACGCCGG 
     3901 GCTCGGGGTA CCTCTGTGCC ATGAAAATCA CCGAGGGCGA CCTGTGGATC CGCACGTACG 
     3961 GCCGCCTCTT CCAGAAGCTC TGCTCCTCCA GCGCCGAGAT CCCCATTGGC ATCTACCGGA 
     4021 CAGAGAGCCA CGTCTTCTCC ACCTCGGAGC CCCACGACCT CAGAGCCCAG TCCCAGATCT 
     4081 CGGTGAACGT GGAGGACTGT GAGGACACAC GGGAAGTGAA GGGGCCCTGG GGCTCCCGCG 
     4141 CTGGCACCGG AGGCAGCTCC CAGGGCCGCC ACACGGGCGG CGGTGACCCC GCAGAGCACC 
     4201 CACTGCTACG GCGCAAGAGC CTGCAGTGGG CCCGGAGGCT GAGCCGCAAG GCGCCCAAGC 
     4261 AGGCAGGCCG GGCGGCGGCC GCGGAGTGGA TCAGCCAGCA GCGCCTCAGC CTGTACCGGC 
     4321 GCTCTGAGCG CCAGGAGCTC TCCGAGCTGG TGAAGAACCG CATGAAGCAC CTGGGGCTGC 
     4381 CCACCACCGG CTACGAGGAC GTAGCAAATT TAACAGCCAG TGATGTCATG AATCGGGTAA 
     4441 ACCTGGGATA TTTGCAAGAC GAGATGAACG ACCACCAGAA CACCCTCTCC TACGTCCTCA 
     4501 TCAACCCTCC GCCCGACACG AGGCTGGAGC CCAGTGACAT TGTCTATCTC ATCCGCTCCG 
     4561 ACCCCCTGGC TCACGTGGCC AGCAGCTCCC AGAGCCGGAA GAGCAGCTGC AGCCACAAGC 
     4621 TGTCGTCCTG CAACCCCGAG ACTCGCGACG AGACACAGCT CACGCGTACG CGGCCGCTCG 
     4681 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4741 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4801 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4861 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4921 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4981 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     5041 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     5101 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5161 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5221 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5281 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5341 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5401 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5461 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5521 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5581 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5641 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5701 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5761 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5821 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     5881 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     5941 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     6001 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     6061 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     6121 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6181 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6241 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6301 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6361 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6421 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6481 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6541 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6601 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6661 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6721 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6781 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6841 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6901 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     6961 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     7021 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     7081 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     7141 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7201 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7261 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7321 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7381 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7441 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7501 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7561 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7621 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7681 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7741 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7801 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7861 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7921 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     7981 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     8041 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     8101 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     8161 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     8221 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8281 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8341 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8401 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8461 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//