LOCUS       RC235216                8514 bp    DNA     circular  SYN 
DEFINITION  KCNMA1 (Myc-DDK tagged) - Homo sapiens potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant 6
ACCESSION   NM_001271519
VERSION     NM_001271519.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4669..4760 
                     /label="Myc-DDK"  
     misc_feature    4790..5375 
                     /label="PolyA signal"  
     misc_feature    4847..4866 
                     /label="Restriction Enzyme"  
     misc_feature    5651..6323 
                     /label="ColE1"  
     misc_feature    6753..7547 
                     /label="Kan/Neo"  
     misc_feature    7582..7884 
                     /label="SV40 ori"  
     misc_feature    7970..8447 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4644..4651 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4644 
                     /label="KCNMA1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCAAATGGT GGCGGCGGCG GCGGCGGCAG CAGCGGCGGC GGCGGCGGCG 
     1081 GCGGAGGCAG CAGTCTTAGA ATGAGTAGCA ATATCCACGC GAACCATCTC AGCCTAGACG 
     1141 CGTCCTCCTC CTCCTCCTCC TCCTCTTCCT CTTCTTCTTC TTCCTCCTCC TCTTCCTCCT 
     1201 CGTCCTCGGT CCACGAGCCC AAGATGGATG CGCTCATCAT CCCGGTGACC ATGGAGGTGC 
     1261 CGTGCGACAG CCGGGGCCAA CGCATGTGGT GGGCTTTCCT GGCCTCCTCC ATGGTGACTT 
     1321 TCTTCGGGGG CCTCTTCATC ATCTTGCTCT GGCGGACGCT CAAGTACCTG TGGACCGTGT 
     1381 GCTGCCACTG CGGGGGCAAG ACGAAGGAGG CCCAGAAGAT TAACAATGGC TCAAGCCAGG 
     1441 CGGATGGCAC TCTCAAACCA GTGGATGAAA AAGAGGAGGC AGTGGCCGCC GAGGTCGGCT 
     1501 GGATGACCTC CGTGAAGGAC TGGGCGGGGG TGATGATATC CGCCCAGACA CTGACTGGCA 
     1561 GAGTCCTGGT TGTCTTAGTC TTTGCTCTCA GCATCGGTGC ACTTGTAATA TACTTCATAG 
     1621 ATTCATCAAA CCCAATAGAA TCCTGCCAGA ATTTCTACAA AGATTTCACA TTACAGATCG 
     1681 ACATGGCTTT CAACGTGTTC TTCCTTCTCT ACTTCGGCTT GCGGTTTATT GCAGCCAACG 
     1741 ATAAATTGTG GTTCTGGCTG GAAGTGAACT CTGTAGTGGA TTTCTTCACG GTGCCCCCCG 
     1801 TGTTTGTGTC TGTGTACTTA AACAGAAGTT GGCTTGGTTT GAGATTTTTA AGAGCTCTGA 
     1861 GACTGATACA GTTTTCAGAA ATTTTGCAGT TTCTGAATAT TCTTAAAACA AGTAATTCCA 
     1921 TCAAGCTGGT GAATCTGCTC TCCATATTTA TCAGCACGTG GCTGACTGCA GCCGGGTTCA 
     1981 TCCATTTGGT GGAGAATTCA GGGGACCCAT GGGAAAATTT CCAAAACAAC CAGGCTCTCA 
     2041 CCTACTGGGA ATGTGTCTAT TTACTCATGG TCACAATGTC CACCGTTGGT TATGGGGATG 
     2101 TTTATGCAAA AACCACACTT GGGCGCCTCT TCATGGTCTT CTTCATCCTC GGGGGACTGG 
     2161 CCATGTTTGC CAGCTACGTC CCTGAAATCA TAGAGTTAAT AGGAAACCGC AAGAAATACG 
     2221 GGGGCTCCTA TAGTGCGGTT AGTGGAAGAA AGCACATTGT GGTCTGCGGA CACATCACTC 
     2281 TGGAGAGTGT TTCCAACTTC CTGAAGGACT TTCTGCACAA GGACCGGGAT GACGTCAATG 
     2341 TGGAGATCGT TTTTCTTCAC AACATCTCCC CCAACCTGGA GCTTGAAGCT CTGTTCAAAC 
     2401 GACATTTTAC TCAGGTGGAA TTTTATCAGG GTTCCGTCCT CAATCCACAT GATCTTGCAA 
     2461 GAGTCAAGAT AGAGTCAGCA GATGCATGCC TGATCCTTGC CAACAAGTAC TGCGCTGACC 
     2521 CGGATGCGGA GGATGCCTCG AATATCATGA GAGTAATCTC CATAAAGAAC TACCATCCGA 
     2581 AGATAAGAAT CATCACTCAA ATGCTGCAGT ATCACAACAA GGCCCATCTG CTAAACATCC 
     2641 CGAGCTGGAA TTGGAAAGAA GGTGATGACG CAATCTGCCT CGCAGAGTTG AAGTTGGGCT 
     2701 TCATAGCCCA GAGCTGCCTG GCTCAAGGCC TCTCCACCAT GCTTGCCAAC CTCTTCTCCA 
     2761 TGAGGTCATT CATAAAGATT GAGGAAGACA CATGGCAGAA ATACTACTTG GAAGGAGTCT 
     2821 CAAATGAAAT GTACACAGAA TATCTCTCCA GTGCCTTCGT GGGTCTGTCC TTCCCTACTG 
     2881 TTTGTGAGCT GTGTTTTGTG AAGCTCAAGC TCCTAATGAT AGCCATTGAG TACAAGTCTG 
     2941 CCAACCGAGA GAGCCGTATA TTAATTAATC CTGGAAACCA TCTTAAGATC CAAGAAGGTA 
     3001 CTTTAGGATT TTTCATCGCA AGTGATGCCA AAGAAGTTAA AAGGGCATTT TTTTACTGCA 
     3061 AGGCCTGTCA TGATGACATC ACAGATCCCA AAAGAATAAA AAAATGTGGC TGCAAACGGC 
     3121 TGATATATTT TGAAGATGAG CAGCCGTCAA CACTATCACC AAAAAAAAAG CAACGGAATG 
     3181 GAGGCATGCG GAACTCACCC AACACCTCGC CTAAGCTGAT GAGGCATGAC CCCTTGTTAA 
     3241 TTCCTGGCAA TGATCAGATT GACAACATGG ACTCCAATGT GAAGAAGTAC GACTCTACTG 
     3301 GGATGTTTCA CTGGTGTGCA CCCAAGGAGA TAGAGAAAGT CATCCTGACT CGAAGTGAAG 
     3361 CTGCCATGAC CGTCCTGAGT GGCCATGTCG TGGTCTGCAT CTTTGGCGAC GTCAGCTCAG 
     3421 CCCTGATCGG CCTCCGGAAC CTGGTGATGC CGCTCCGTGC CAGCAACTTT CATTACCATG 
     3481 AGCTCAAGCA CATTGTGTTT GTGGGCTCTA TTGAGTACCT CAAGCGGGAA TGGGAGACGC 
     3541 TTCATAACTT CCCCAAAGTG TCCATATTGC CTGGTACGCC ATTAAGTCGG GCTGATTTAA 
     3601 GGGCTGTCAA CATCAACCTC TGTGACATGT GCGTTATCCT GTCAGCCAAT CAGAATAATA 
     3661 TTGATGATAC TTCGCTGCAG GACAAGGAAT GCATCTTGGC GTCACTCAAC ATCAAATCTA 
     3721 TGCAGTTTGA TGACAGCATC GGAGTCTTGC AGGCTAATTC CCAAGGGTTC ACACCTCCAG 
     3781 GAATGGATAG ATCCTCTCCA GATAACAGCC CAGTGCACGG GATGTTACGT CAACCATCCA 
     3841 TCACAACTGG GGTCAACATC CCCATCATCA CTGAACTAGC TAAACCGGGC AAGTTGCCTT 
     3901 TGGTATCAGT CAATCAGGAA AAAAACAGTG GGACGCACAT TCTAATGATA ACTGAACTGG 
     3961 TGAACGATAC TAATGTTCAG TTTTTGGACC AAGACGATGA TGATGACCCT GATACAGAAC 
     4021 TGTACCTCAC GCAGCCCTTT GCCTGTGGGA CAGCATTTGC CGTCAGTGTC CTGGACTCAC 
     4081 TCATGAGCGC GACGTACTTC AATGACAATA TCCTCACCCT GATACGGACC CTGGTGACCG 
     4141 GAGGAGCCAC GCCGGAGCTG GAGGCTCTGA TTGCTGAGGA AAACGCCCTT AGAGGTGGCT 
     4201 ACAGCACCCC GCAGACACTG GCCAATAGGG ACCGCTGCCG CGTGGCCCAG TTAGCTCTGC 
     4261 TCGATGGGCC ATTTGCGGAC TTAGGGGATG GTGGTTGTTA TGGTGATCTG TTCTGCAAAG 
     4321 CTCTGAAAAC ATATAATATG CTTTGTTTTG GAATTTACCG GCTGAGAGAT GCTCACCTCA 
     4381 GCACCCCCAG TCAGTGCACA AAGAGGTATG TCATCACCAA CCCGCCCTAT GAGTTTGAGC 
     4441 TCGTGCCGAC GGACCTGATC TTCTGCTTAA TGCAGTTTGA CCACAATGCC GGCCAGTCCC 
     4501 GGGCCAGCCT GTCCCATTCC TCCCACTCGT CGCAGTCCTC CAGCAAGAAG AGCTCCTCTG 
     4561 TTCACTCCAT CCCATCCACA GCAAACCGAC AGAACCGGCC CAAGTCCAGG GAGTCCCGGG 
     4621 ACAAACAGAA GTACGTGCAG GAAGAGCGGC TTAGCGGACC GACGCGTACG CGGCCGCTCG 
     4681 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4741 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4801 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4861 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4921 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4981 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     5041 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     5101 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5161 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5221 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5281 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5341 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5401 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5461 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5521 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5581 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5641 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5701 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5761 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5821 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     5881 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     5941 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     6001 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     6061 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     6121 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6181 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6241 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6301 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6361 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6421 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6481 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6541 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6601 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6661 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6721 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6781 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6841 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6901 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     6961 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     7021 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     7081 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     7141 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7201 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7261 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7321 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7381 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7441 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7501 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7561 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7621 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7681 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7741 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7801 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7861 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7921 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     7981 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     8041 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     8101 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     8161 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     8221 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8281 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8341 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8401 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8461 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//