LOCUS       RC235214                8496 bp    DNA     circular  SYN 
DEFINITION  SEMA5B (Myc-DDK tagged) - Homo sapiens sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B (SEMA5B), transcript variant 3
ACCESSION   NM_001256347
VERSION     NM_001256347.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4653..4744 
                     /label="Myc-DDK"  
     misc_feature    4774..5359 
                     /label="PolyA signal"  
     misc_feature    4831..4850 
                     /label="Restriction Enzyme"  
     misc_feature    5635..6307 
                     /label="ColE1"  
     misc_feature    6737..7531 
                     /label="Kan/Neo"  
     misc_feature    7566..7868 
                     /label="SV40 ori"  
     misc_feature    7954..8431 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4635..4641 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4635 
                     /label="SEMA5B"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTGCATCTG TCAGCAGAGG AAGCCATTGG CTGCGTGAGG GTCAGAAGGA 
     1081 GCTTCATAGA TGAGCTGGCA TTTGGGAGAG GGCATTCTAC AGGGACGGGC AAGCAGAAGC 
     1141 GAAGGGATCG AGTGAGTGGT TCCAGCTGGT GCCTGGCCTG TGTCTCTTGG ATGCCCTGTG 
     1201 GCTTCAGTCC GTCTCCTGTT GCCCACCACC TCGTCCCTGG GCCGCCTGAT ACCCCAGCCC 
     1261 AACAGCTAAG GTGTGGATGG ACAGTAGGGG GCTGGCTTCT CTCACTGGTC AGGGGTCTTC 
     1321 TCCCCTGTCT GCCTCCCGGA GCTAGGACTG CAGAGGGGCC TATCATGGTG CTTGCAGGCC 
     1381 CCCTGGCTGT CTCGCTGTTG CTGCCCAGCC TCACACTGCT GGTGTCCCAC CTCTCCAGCT 
     1441 CCCAGGATGT CTCCAGTGAG CCCAGCAGTG AGCAGCAGCT GTGCGCCCTT AGCAAGCACC 
     1501 CCACCGTGGC CTTTGAAGAC CTGCAGCCGT GGGTCTCTAA CTTCACCTAC CCTGGAGCCC 
     1561 GGGATTTCTC CCAGCTGGCT TTGGACCCCT CCGGGAACCA GCTCATCGTG GGAGCCAGGA 
     1621 ACTACCTCTT CAGACTCAGC CTTGCCAATG TCTCTCTTCT TCAGGCCACA GAGTGGGCCT 
     1681 CCAGTGAGGA CACGCGCCGC TCCTGCCAAA GCAAAGGGAA GACTGAGGAG GAGTGTCAGA 
     1741 ACTACGTGCG AGTCCTGATC GTCGCCGGCC GGAAGGTGTT CATGTGTGGA ACCAATGCCT 
     1801 TTTCCCCCAT GTGCACCAGC AGACAGGTGG GGAACCTCAG CCGGACTATT GAGAAGATCA 
     1861 ATGGTGTGGC CCGCTGCCCC TATGACCCAC GCCACAACTC CACAGCTGTC ATCTCCTCCC 
     1921 AGGGGGAGCT CTATGCAGCC ACGGTCATCG ACTTCTCAGG TCGGGACCCT GCCATCTACC 
     1981 GCAGCCTGGG CAGTGGGCCA CCGCTTCGCA CTGCCCAATA TAACTCCAAG TGGCTTAATG 
     2041 AGCCAAACTT CGTGGCAGCC TATGATATTG GGCTGTTTGC ATACTTCTTC CTGCGGGAGA 
     2101 ACGCAGTGGA GCACGACTGT GGACGCACCG TGTACTCTCG CGTGGCCCGC GTGTGCAAGA 
     2161 ATGACGTGGG GGGCCGATTC CTGCTGGAGG ACACATGGAC CACATTCATG AAGGCCCGGC 
     2221 TCAACTGCTC CCGCCCGGGC GAGGTCCCCT TCTACTATAA CGAGCTGCAG AGTGCCTTCC 
     2281 ACTTGCCGGA GCAGGACCTC ATCTATGGAG TTTTCACAAC CAACGTAAAC AGCATCGCGG 
     2341 CTTCTGCTGT CTGCGCCTTC AACCTCAGTG CTATCTCCCA GGCTTTCAAT GGCCCATTTC 
     2401 GCTACCAGGA GAACCCCAGG GCTGCCTGGC TCCCCATAGC CAACCCCATC CCCAATTTCC 
     2461 AGTGTGGCAC CCTGCCTGAG ACCGGTCCCA ACGAGAACCT GACGGAGCGC AGCCTGCAGG 
     2521 ACGCGCAGCG CCTCTTCCTG ATGAGCGAGG CCGTGCAGCC GGTGACACCC GAGCCCTGTG 
     2581 TCACCCAGGA CAGCGTGCGC TTCTCACACC TCGTGGTGGA CCTGGTGCAG GCTAAAGACA 
     2641 CGCTCTACCA TGTACTCTAC ATTGGCACCG AGTCGGGCAC CATCCTGAAG GCGCTGTCCA 
     2701 CGGCGAGCCG CAGCCTCCAC GGCTGCTACC TGGAGGAGCT GCACGTGCTG CCCCCCGGGC 
     2761 GCCGCGAGCC CCTGCGCAGC CTGCGCATCC TGCACAGCGC CCGCGCGCTC TTCGTGGGGC 
     2821 TGAGAGACGG CGTCCTGCGG GTCCCACTGG AGAGGTGCGC CGCCTACCGC AGCCAGGGGG 
     2881 CATGCCTGGG GGCCCGGGAC CCGTACTGTG GCTGGGACGG GAAGCAGCAA CGTTGCAGCA 
     2941 CACTCGAGGA CAGCTCCAAC ATGAGCCTCT GGACCCAGAA CATCACCGCC TGTCCTGTGC 
     3001 GGAATGTGAC ACGGGATGGG GGCTTCGGCC CATGGTCACC ATGGCAACCA TGTGAGCACT 
     3061 TGGATGGGGA CAACTCAGGC TCTTGCCTGT GTCGAGCTCG ATCCTGTGAT TCCCCTCGAC 
     3121 CCCGCTGTGG GGGCCTTGAC TGCCTGGGGC CAGCCATCCA CATCGCCAAC TGCTCCAGGA 
     3181 ATGGGGCGTG GACCCCGTGG TCATCGTGGG CGCTGTGCAG CACGTCCTGT GGCATCGGCT 
     3241 TCCAGGTCCG CCAGCGAAGT TGCAGCAACC CTGCTCCCCG CCACGGGGGC CGCATCTGCG 
     3301 TGGGCAAGAG CCGGGAGGAA CGGTTCTGTA ATGAGAACAC GCCTTGCCCG GTGCCCATCT 
     3361 TCTGGGCTTC CTGGGGCTCC TGGAGCAAGT GCAGCAGCAA CTGTGGAGGG GGCATGCAGT 
     3421 CGCGGCGTCG GGCCTGCGAG AACGGCAACT CCTGCCTGGG CTGCGGCGTG GAGTTCAAGA 
     3481 CGTGCAACCC CGAGGGCTGC CCCGAAGTGC GGCGCAACAC CCCCTGGACG CCGTGGCTGC 
     3541 CCGTGAACGT GACGCAGGGC GGGGCACGGC AGGAGCAGCG GTTCCGCTTC ACCTGCCGCG 
     3601 CGCCCCTTGC AGACCCGCAC GGCCTGCAGT TCGGCAGGAG AAGGACCGAG ACGAGGACCT 
     3661 GTCCCGCGGA CGGCTCCGGC TCCTGCGACA CCGACGCCCT GGTGGAGGTC CTCCTGCGCA 
     3721 GCGGGAGCAC CTCCCCGCAC ACGGTGAGCG GGGGCTGGGC CGCCTGGGGC CCGTGGTCGT 
     3781 CCTGCTCCCG GGACTGCGAG CTGGGCTTCC GCGTCCGCAA GAGAACGTGC ACTAACCCGG 
     3841 AGCCCCGCAA CGGGGGCCTG CCCTGCGTGG GCGATGCTGC CGAGTACCAG GACTGCAACC 
     3901 CCCAGGCTTG CCCAGTTCGG GGTGCTTGGT CCTGCTGGAC CTCATGGTCT CCATGCTCAG 
     3961 CTTCCTGTGG TGGGGGTCAC TATCAACGCA CCCGTTCCTG CACCAGCCCC GCACCCTCCC 
     4021 CAGGTGAGGA CATCTGTCTC GGGCTGCACA CGGAGGAGGC ACTATGTGCC ACACAGGCCT 
     4081 GCCCAGAAGG CTGGTCGCCC TGGTCTGAGT GGAGTAAGTG CACTGACGAC GGAGCCCAGA 
     4141 GCCGAAGCCG GCACTGTGAG GAGCTCCTCC CAGGGTCCAG CGCCTGTGCT GGAAACAGCA 
     4201 GCCAGAGCCG CCCCTGCCCC TACAGCGAGA TTCCCGTCAT CCTGCCAGCC TCCAGCATGG 
     4261 AGGAGGCCAC CGACTGTGCA GGGTTCAATC TCATCCACTT GGTGGCCACG GGCATCTCCT 
     4321 GCTTCTTGGG CTCTGGGCTC CTGACCCTAG CAGTGTACCT GTCTTGCCAG CACTGCCAGC 
     4381 GTCAGTCCCA GGAGTCCACA CTGGTCCATC CTGCCACCCC CAACCATTTG CACTACAAGG 
     4441 GCGGAGGCAC CCCGAAGAAT GAAAAGTACA CACCCATGGA ATTCAAGACC CTGAACAAGA 
     4501 ATAACTTGAT CCCTGATGAC AGAGCCAACT TCTACCCATT GCAGCAGACC AATGTGTACA 
     4561 CGACTACTTA CTACCCAAGC CCCCTGAACA AACACAGCTT CCGGCCCGAG GCCTCACCTG 
     4621 GACAACGGTG CTTCCCCAAC AGCACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4681 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4741 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4801 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4861 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4921 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4981 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     5041 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     5101 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     5161 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     5221 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     5281 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5341 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5401 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     5461 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     5521 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     5581 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5641 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     5701 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5761 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     5821 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     5881 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     5941 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     6001 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     6061 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     6121 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     6181 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     6241 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     6301 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     6361 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     6421 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     6481 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6541 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6601 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6661 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6721 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6781 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     6841 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     6901 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     6961 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     7021 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     7081 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     7141 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     7201 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     7261 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     7321 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     7381 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     7441 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     7501 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7561 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7621 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7681 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7741 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7801 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7861 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     7921 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     7981 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     8041 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     8101 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     8161 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     8221 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     8281 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     8341 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     8401 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     8461 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//