LOCUS       RC235210                8448 bp    DNA     circular  SYN 
DEFINITION  NEK1 (Myc-DDK tagged) - Homo sapiens NIMA-related kinase 1 (NEK1), transcript variant 4
ACCESSION   NM_001199399
VERSION     NM_001199399.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4605..4696 
                     /label="Myc-DDK"  
     misc_feature    4726..5311 
                     /label="PolyA signal"  
     misc_feature    4783..4802 
                     /label="Restriction Enzyme"  
     misc_feature    5587..6259 
                     /label="ColE1"  
     misc_feature    6689..7483 
                     /label="Kan/Neo"  
     misc_feature    7518..7820 
                     /label="SV40 ori"  
     misc_feature    7906..8383 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4587..4593 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4587 
                     /label="NEK1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGAAGTAT GTTAGACTAC AGAAGATTGG AGAAGGTTCA TTTGGAAAAG 
     1081 CCATTCTTGT TAAATCTACA GAAGATGGCA GACAGTATGT TATCAAGGAA ATTAACATCT 
     1141 CAAGAATGTC CAGTAAAGAA AGAGAAGAAT CAAGGAGAGA AGTTGCAGTA TTGGCAAACA 
     1201 TGAAGCATCC AAATATTGTC CAGTATAGAG AATCATTTGA AGAAAATGGC TCTCTCTACA 
     1261 TAGTAATGGA TTACTGTGAG GGAGGGGATC TGTTTAAGCG AATAAATGCT CAGAAAGGCG 
     1321 TTTTGTTTCA AGAGGATCAG ATTTTGGACT GGTTTGTACA GATATGTTTG GCCCTGAAAC 
     1381 ATGTACATGA TAGAAAAATT CTTCATCGAG ACATTAAATC TCAGAACATA TTTTTAACTA 
     1441 AAGATGGAAC AGTACAACTT GGAGATTTTG GAATTGCTAG AGTTCTTAAT AGTACTGTAG 
     1501 AGCTGGCTCG AACTTGCATA GGGACCCCAT ACTACTTGTC ACCTGAAATC TGTGAAAACA 
     1561 AACCTTACAA TAATAAAAGT GACATTTGGG CTCTGGGGTG TGTCCTTTAT GAGCTGTGTA 
     1621 CACTTAAACA TGCTTTTGAA GCTGGCAGTA TGAAAAACCT GGTACTGAAG ATAATATCTG 
     1681 GATCTTTTCC ACCTGTGTCT TTGCATTATT CCTATGATCT CCGCAGTTTG GTGTCTCAGT 
     1741 TATTTAAAAG AAATCCTAGG GATAGACCAT CAGTCAACTC CATATTGGAG AAAGGTTTTA 
     1801 TAGCCAAACG CATTGAAAAG TTTCTCTCTC CTCAGCTTAT TGCAGAAGAA TTTTGTCTAA 
     1861 AAACATTTTC GAAGTTTGGA TCACAGCCTA TACCAGCTAA AAGACCAGCT TCAGGACAAA 
     1921 ACTCGATTTC TGTTATGCCT GCTCAGAAAA TTACAAAGCC TGCCGCTAAA TATGGAATAC 
     1981 CTTTAGCATA TAAGAAATAT GGAGATAAAA AATTACACGA AAAGAAACCA CTGCAAAAAC 
     2041 ATAAACAGGC CCATCAAACT CCAGAGAAGA GAGTGAATAC TGGAGAAGAA AGGAGGAAAA 
     2101 TATCTGAGGA AGCAGCAAGA AAGAGAAGGC TGGAATTTAT TGAAAAAGAA AAGAAACAAA 
     2161 AGGATCAGAT TATTAGTTTA ATGAAGGCTG AACAAATGAA AAGGCAAGAA AAGGAAAGGG 
     2221 CTCCTTTTCT GGGCAGTGGA GGGACTATAG CTCCATCATC TTTTTCTTCT CGAGGACAGT 
     2281 ATGAACATTA CCATGCCATT TTTGACCAAA TGCAGCAACA AAGAGCAGAA GATAATGAAG 
     2341 CTAAATGGAA AAGAGAAATA TATGGTCGAG GTCTTCCAGA AAGGCAAAAA GGGCAGCTAG 
     2401 CTGTAGAAAG AGCTAAACAA GTAGAAGAGT TCCTGCAGCG AAAACGGGAA GCTATGCAGA 
     2461 ATAAAGCTCG AGCCGAAGGA CATATGGTTT ATCTGGCAAG ACTGAGGCAA ATAAGACTAC 
     2521 AGAATTTCAA TGAGCGCCAA CAGATTAAAG CCAAACTTCG TGGTGAAAAG AAAGAAGCTA 
     2581 ATCATTCTGA AGGACAAGAA GGAAGTGAAG AGGCTGACAT GAGGCGCAAA AAAATCGAAT 
     2641 CACTGAAGGC CCATGCAAAT GCACGTGCTG CTGTACTAAA AGAACAACTA GAACGAAAGA 
     2701 GAAAGGAGGC TTATGAGAGA GAAAAAAAAG TGTGGGAAGA GCATTTGGTG GCTAAAGGAG 
     2761 TTAAGAGTTC TGATGTTTCT CCACCTTTGG GACAGCATGA AACAGGTGGC TCTCCATCAA 
     2821 AGCAACAGAT GAGATCTGTT ATTTCTGTAA CTTCAGCTTT GAAAGAAGTT GGCGTGGACA 
     2881 GTAGTTTAAC TGATACCCGG GAAACTTCAG AAGAGATGCA AAAGACCAAC AATGCTATTT 
     2941 CAAGTAAGCG AGAAATACTT CGTAGATTAA ATGAAAATCT TAAAGCTCAA GAAGATGAAA 
     3001 AAGGAAAGCA GAATCTCTCT GATACTTTTG AGATAAATGT TCATGAAGAT GCCAAAGAGC 
     3061 ATGAAAAAGA AAAATCAGTT TCATCTGATC GCAAGAAGTG GGAGGCAGGA GGTCAACTTG 
     3121 TGATTCCTCT GGATGAGTTA ACACTAGATA CATCCTTCTC TACAACTGAA AGACATACAG 
     3181 TGGGAGAAGT TATTAAATTA GGTCCTAATG GATCTCCAAG AAGAGCCTGG GGGAAAAGTC 
     3241 CGACAGATTC TGTTCTAAAG ATACTTGGAG AAGCTGAACT ACAACTTCAG ACAGAACTAT 
     3301 TAGAAAATAC AACTATTAGA AGTGAGATTT CTCCCGAAGG GGAAAAGTAC AAACCCTTAA 
     3361 TTACTGGAGA AAAAAAAGTA CAATGTATTT CACATGAAAT AAACCCATCA GCTATTGTTG 
     3421 ATTCTCCTGT TGAGACAAAA AGTCCCGAGT TCAGTGAGGC ATCTCCACAG ATGTCATTGA 
     3481 AACTGGAAGG AAATTTAGAA GAACCTGATG ATTTGGAAAC AGAAATTCTA CAAGAGCCAA 
     3541 GTGGAACAAA CAAAGATGAG AGCTTGCCAT GCACTATTAC TGATGTGTGG ATTAGTGAGG 
     3601 AAAAAGAAAC AAAGGAAACT CAGTCGGCAG ATAGGATCAC CATTCAGGAA AATGAAGTTT 
     3661 CTGAAGATGG AGTCTCGAGT ACTGTGGACC AACTTAGTGA CATTCATATA GAGCCTGGAA 
     3721 CCAATGATTC TCAGCACTCT AAATGTGATG TAGATAAGTC TGTGCAACCG GAACCATTTT 
     3781 TCCATAAGGT GGTTCATTCT GAACACTTGA ACTTAGTCCC TCAAGTTCAA TCAGTTCAGT 
     3841 GTTCACCAGA AGAATCCTTT GCATTTCGAT CTCACTCGCA TTTACCACCA AAAAATAAAA 
     3901 ACAAGAATTC CTTGCTGATT GGACTTTCAA CTGGTCTGTT TGATGCAAAC AACCCAAAGA 
     3961 TGTTAAGGAC ATGTTCACTT CCAGATCTCT CAAAGCTGTT CAGAACCCTT ATGGATGTTC 
     4021 CCACCGTAGG AGATGTTCGT CAAGACAATC TTGAAATAGA TGAAATTGAA GATGAAAACA 
     4081 TTAAAGAAGG ACCTTCTGAT TCTGAAGACA TTGTGTTTGA AGAAACTGAC ACAGATTTAC 
     4141 AAGAGCTGCA GGCCTCGATG GAACAGTTAC TTAGGGAACA ACCTGGTGAA GAATACAGTG 
     4201 AAGAAGAAGA GTCAGTCTTG AAGAACAGTG ATGTGGAGCC AACTGCAAAT GGGACAGATG 
     4261 TGGCAGATGA AGATGACAAT CCCAGCAGTG AAAGTGCCCT GAACGAAGAA TGGCACTCAG 
     4321 ATAACAGTGA TGGTGAAATT GCTAGTGAAT GTGAATGCGA TAGTGTCTTT AACCATTTAG 
     4381 AGGAACTGAG ACTTCATCTG GAGCAGGAAA TGGGCTTTGA AAAATTCTTT GAGGTTTATG 
     4441 AGAAAATAAA GGCTATTCAT GAAGATGAAG ATGAAAATAT TGAAATTTGT TCAAAAATAG 
     4501 TTCAAAATAT TTTGGGAAAT GAACATCAGC ATCTTTATGC CAAGATTCTT CATTTAGTCA 
     4561 TGGCAGATGG AGCCTACCAA GAAGATAATG ATGAAACGCG TACGCGGCCG CTCGAGCAGA 
     4621 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     4681 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     4741 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     4801 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     4861 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     4921 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     4981 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     5041 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     5101 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     5161 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     5221 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     5281 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     5341 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     5401 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     5461 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     5521 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     5581 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     5641 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     5701 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     5761 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     5821 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     5881 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     5941 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     6001 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     6061 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     6121 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     6181 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     6241 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     6301 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     6361 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     6421 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     6481 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     6541 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     6601 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     6661 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     6721 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     6781 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     6841 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     6901 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     6961 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     7021 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     7081 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     7141 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     7201 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     7261 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     7321 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     7381 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     7441 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     7501 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     7561 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     7621 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     7681 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7741 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     7801 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     7861 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     7921 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     7981 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     8041 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     8101 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     8161 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     8221 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     8281 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     8341 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     8401 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//