LOCUS       RC235208                8427 bp    DNA     circular  SYN 
DEFINITION  DIAPH3 (Myc-DDK tagged) - Homo sapiens diaphanous-related formin 3 (DIAPH3), transcript variant 3
ACCESSION   NM_001258366
VERSION     NM_001258366.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4584..4675 
                     /label="Myc-DDK"  
     misc_feature    4705..5290 
                     /label="PolyA signal"  
     misc_feature    4762..4781 
                     /label="Restriction Enzyme"  
     misc_feature    5566..6238 
                     /label="ColE1"  
     misc_feature    6668..7462 
                     /label="Kan/Neo"  
     misc_feature    7497..7799 
                     /label="SV40 ori"  
     misc_feature    7885..8362 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4566..4572 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4566 
                     /label="DIAPH3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAACGGCAC CAGCCGCGGC TGCACCACCC GGCCCAAGGC TCAGCCGCTG 
     1081 GGACTCCCTA CCCTTCCTCA GCCTCTCTCC GCGGCTGCCG GGAAAGCAAG ATGCCGCGCA 
     1141 GGAAGGGCCC CCAACACCCT CCGCCGCCCA GTGGCCCCGA GGAGCCTGGG GAGAAGCGCC 
     1201 CCAAGTTTCT GGACAAATTT GCCAGCATAA GAATTCCAGG GAGCAAGAAA GAGAGACCTC 
     1261 CACTTCCCAA CCTGAAGACT GCATTTGCAA GCAGTGATTG CTCAGCAGCA CCTTTAGAGA 
     1321 TGATGGAGAA CTTTCCAAAG CCACTGTCAG AGAATGAACT CTTAGAACTT TTTGAAAAAA 
     1381 TGATGGAAGA TATGAATTTA AATGAAGATA AAAAGGCACC ATTGCGGGAA AAGGACTTCA 
     1441 GTATCAAAAA AGAAATGGTG ATGCAGTACA TTAATACTGC TTCTAAGACA GGAAGTCTTA 
     1501 AGAGAAGCCG ACAGATCTCA CCTCAGGAAT TCATTCATGA GCTGAAAATG GGGTCTGCAG 
     1561 ATGAGAGACT TGTCACATGC CTGGAGTCTC TCCGAGTGTC TTTGACCAGC AATCCTGTGA 
     1621 GTTGGGTGGA AAGCTTTGGA CATGAAGGGC TTGGATTATT ATTAGACATT TTGGAAAAAC 
     1681 TGATTAGTGG AAAAATCCAA GAAAAAGTTG TAAAGAAAAA TCAACATAAA GTCATACAGT 
     1741 GTCTAAAAGC CCTGATGAAT ACGCAGTATG GCTTGGAAAG AATTATGAGT GAGGAGAGGA 
     1801 GCCTTTCCTT ATTGGCCAAA GCCGTGGATC CCAGACACCC CAATATGATG ACAGATGTGG 
     1861 TTAAACTTCT CTCTGCGGTA TGCATTGTAG GGGAAGAAAG CATCCTTGAA GAAGTTTTAG 
     1921 AAGCTTTAAC TTCAGCTGGT GAAGAAAAAA AAATTGACAG ATTTTTTTGT ATTGTGGAAG 
     1981 GCCTCCGGCA CAATTCAGTT CAACTGCAAG TAGCTTGTAT GCAGCTCATC AATGCCCTGG 
     2041 TTACATCTCC TGATGATTTG GATTTCAGGC TTCACATCAG AAATGAATTT ATGCGTTGTG 
     2101 GATTGAAAGA GATATTGCCA AATTTAAAAT GCATTAAGAA TGATGGCCTG GATATCCAAC 
     2161 TTAAAGTCTT TGATGAGCAT AAAGAAGAAG ATTTGTTTGA GTTATCCCAT CGCCTTGAAG 
     2221 ATATTAGAGC TGAACTTGAT GAAGCATATG ATGTTTACAA CATGGTGTGG AGCACAGTTA 
     2281 AAGAAACTAG AGCAGAGGGA TATTTTATTT CTATTCTTCA GCATCTTTTG CTGATTCGAA 
     2341 ATGATTATTT TATAAGGCAA CAATACTTCA AATTAATTGA TGAGTGTGTA TCCCAGATTG 
     2401 TATTGCATAG AGATGGAATG GATCCAGACT TCACATATCG AAAAAGACTA GATTTAGATT 
     2461 TAACCCAGTT TGTAGACATT TGCATAGATC AAGCAAAACT AGAAGAGTTT GAAGAGAAAG 
     2521 CATCAGAACT TTACAAGAAA TTTGAAAAAG AGTTTACCGA CCACCAAGAA ACTCAGGCTG 
     2581 AATTGCAGAA AAAAGAGGCA AAGATTAATG AGCTTCAAGC AGAGCTACAA GCTTTTAAGT 
     2641 CTCAGTTTGG TGCCTTGCCA GCTGATTGTA ATATTCCTTT GCCTCCCTCT AAAGAAGGTG 
     2701 GAACTGGCCA CTCAGCACTT CCTCCTCCGC CTCCACTGCC TTCTGGTGGA GGGGTGCCGC 
     2761 CTCCACCTCC TCCCCCACCA CCTCCTCCAC TTCCAGGAAT GCGGATGCCA TTCAGTGGTC 
     2821 CTGTGCCTCC ACCACCTCCC CTGGGATTCC TTGGAGGACA AAATTCTCCT CCTCTACCAA 
     2881 TCCTGCCATT TGGGTTGAAA CCAAAGAAAG AATTTAAACC TGAAATCAGC ATGAGAAGAT 
     2941 TGAATTGGTT AAAGATCAGA CCTCATGAAA TGACTGAAAA CTGTTTCTGG ATAAAAGTAA 
     3001 ATGAAAATAA GTATGAAAAC GTGGATTTGC TTTGTAAACT TGAGAATACA TTTTGTTGCC 
     3061 AACAAAAAGA GAGAAGAGAA GAGGAAGATA TTGAAGAGAA GAAATCGATT AAGAAAAAAA 
     3121 TTAAAGAACT TAAGTTTTTA GATTCTAAAA TTGCCCAGAA CCTTTCAATC TTCCTGAGCT 
     3181 CTTTTCGGGT GCCATATGAG GAAATCAGAA TGATGATATT GGAAGTAGAT GAAACACGGT 
     3241 TGGCAGAGTC TATGATTCAG AACTTAATAA AGCATCTTCC TGATCAAGAG CAATTAAATT 
     3301 CATTGTCTCA GTTCAAGAGT GAATATAGCA ACTTATGTGA ACCTGAGCAG TTTGTGGTTG 
     3361 TGATGAGCAA TGTGAAGAGA CTACGGCCAC GGCTCAGTGC TATTCTCTTT AAGCTTCAGT 
     3421 TTGAAGAGCA GGTGAACAAC ATCAAACCTG ACATCATGGC TGTCAGTACT GCCTGCGAAG 
     3481 AGATAAAGAA GAGCAAAAGC TTTAGCAAGT TGCTGGAACT TGTATTGCTA ATGGGAAACT 
     3541 ACATGAATGC TGGCTCCCGG AATGCTCAAA CCTTCGGATT TAACCTTAGC TCTCTCTGTA 
     3601 AACTAAAGGA CACAAAATCA GCAGATCAGA AAACAACGCT ACTTCATTTC CTGGTAGAAA 
     3661 TATGTGAAGA GAAGTACCCT GATATACTGA ATTTTGTGGA TGATTTGGAA CCTTTAGACA 
     3721 AAGCTAGTAA AGTCTCTGTA GAAACGCTGG AAAAGAATTT GAGGCAGATG GGAAGGCAGC 
     3781 TTCAACAGCT TGAGAAGGAA TTGGAAACCT TTCCCCCTCC TGAGGACTTG CATGACAAGT 
     3841 TTGTGACAAA GATGTCCAGA TTTGTTATCA GTGCAAAAGA ACAATATGAG ACACTTTCGA 
     3901 AGTTACACGA AAACATGGAA AAGTTATACC AGAGTATAAT AGGATACTAT GCCATTGATG 
     3961 TGAAGAAGGT GTCTGTGGAA GACTTTCTTA CTGACCTGAA TAACTTCAGA ACCACATTCA 
     4021 TGCAAGCAAT AAAGGAGAAT ATCAAAAAAA GAGAAGCAGA GGAAAAAGAA AAACGTGTCA 
     4081 GAATAGCTAA AGAATTAGCA GAGCGAGAAA GACTCGAACG CCAACAAAAG AAAAAGCGTT 
     4141 TATTAGAAAT GAAGACTGAG GGTGATGAGA CAGGAGTGAT GGATAATCTG CTGGAGGCCT 
     4201 TGCAGTCCGG GGCTGCCTTC CGCGACAGAA GAAAAAGGAC ACCGATGCCA AAAGATGTTC 
     4261 GGCAGAGTCT CAGTCCAATG TCTCAGAGGC CTGTTCTGAA AGTTTGTAAC CATGAAAATC 
     4321 AGAAAGTGCA GTTGACAGAA GGGTCACGTT CACACTACAA TATCAATTGC AACTCAACAA 
     4381 GGACTCCAGT CGCCAAGGAG CTTAATTATA ATCTAGACAC TCATACGTCT ACTGGGAGGA 
     4441 TCAAGGCAGC TGAGAAGAAG GAAGCGTGTA ATGTAGAAAG CAACAGAAAA AAGGAAACGG 
     4501 AACTTCTTGG CTCTTTTTCT AAAAATGAAT CAGTTCCCGA AGTTGAAGCC CTGCTGGCAA 
     4561 GATTACGAGC TTTAACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     4621 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     4681 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4741 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     4801 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     4861 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     4921 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     4981 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     5041 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     5101 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     5161 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     5221 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     5281 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     5341 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     5401 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     5461 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     5521 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     5581 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     5641 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     5701 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     5761 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     5821 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     5881 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     5941 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     6001 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     6061 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     6121 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     6181 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     6241 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     6301 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     6361 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     6421 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     6481 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     6541 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     6601 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     6661 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     6721 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     6781 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     6841 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     6901 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     6961 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     7021 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     7081 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     7141 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     7201 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     7261 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     7321 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     7381 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     7441 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     7501 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     7561 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     7621 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     7681 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7741 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7801 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     7861 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     7921 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     7981 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     8041 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     8101 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     8161 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     8221 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     8281 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     8341 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     8401 ATTTAACAAA AATTTAACGC GAATTTT
//