LOCUS       RC235204                8400 bp    DNA     circular  SYN 
DEFINITION  MYBPC1 (Myc-DDK tagged) - Homo sapiens myosin binding protein C, slow type (MYBPC1), transcript variant 5
ACCESSION   NM_001254718
VERSION     NM_001254718.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4557..4648 
                     /label="Myc-DDK"  
     misc_feature    4678..5263 
                     /label="PolyA signal"  
     misc_feature    4735..4754 
                     /label="Restriction Enzyme"  
     misc_feature    5539..6211 
                     /label="ColE1"  
     misc_feature    6641..7435 
                     /label="Kan/Neo"  
     misc_feature    7470..7772 
                     /label="SV40 ori"  
     misc_feature    7858..8335 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4539..4545 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4539 
                     /label="MYBPC1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCAGAACCC ACTAAGAAAG AGGAAAATGA AGTGCCAGCC CCAGCCCCAC 
     1081 CCCCGGAAGA ACCAAGTAAA GAGAAGGAGG CCGGAACTAC ACCAGCAAAA GACTGGACCC 
     1141 TTGTCGAAAC TCCTCCTGGG GAGGAACAAG CCAAGCAGAA TGCCAACTCC CAGCTGTCCA 
     1201 TCTTGTTCAT TGAAAAACCT CAAGGAGGAA CAGTGAAAGT TGGTGAAGAT ATCACCTTCA 
     1261 TAGCCAAAGT CAAGGCTGAA GATCTTCTGA GAAAACCCAC TATCAAATGG TTCAAAGGAA 
     1321 AATGGATGGA CCTGGCCAGC AAAGCCGGGA AGCACCTTCA GCTGAAGGAA ACCTTTGAGA 
     1381 GGCACAGTCG GGTGTACACA TTTGAGATGC AGATCATCAA GGCCAAAGAT AACTTTGCAG 
     1441 GAAATTACAG ATGCGAGGTC ACCTATAAGG ATAAGTTTGA CAGCTGTTCA TTTGATCTTG 
     1501 AAGTGCACGA ATCTACTGGG ACTACTCCAA ACATTGACAT CAGATCTGCT TTCAAGAGAA 
     1561 GTGGAGAAGG TCAAGAGGAT GCAGGAGAAC TTGACTTTAG TGGTCTCCTG AAACGTAGGG 
     1621 AGGTGAAGCA GCAGGAGGAA GAACCCCAGG TGGACGTATG GGAGTTGCTG AAGAACGCGA 
     1681 AACCCAGTGA GTACGAGAAG ATCGCCTTCC AGTATGGAAT CACCGACCTG CGCGGCATGC 
     1741 TCAAGCGACT CAAGCGCATG CGCAGAGAGG AGAAGAAGAG CGCAGCTTTT GCAAAAATTC 
     1801 TTGATCCTGC ATATCAGGTT GACAAAGGAG GCAGAGTGAG GTTTGTTGTG GAGCTGGCAG 
     1861 ATCCAAAGTT GGAGGTGAAA TGGTATAAAA ATGGTCAAGA AATTCGACCC AGTACCAAAT 
     1921 ACATCTTTGA ACACAAAGGA TGCCAGAGAA TCCTGTTTAT CAATAACTGT CAGATGACAG 
     1981 ATGATTCAGA GTATTATGTG ACAGCCGGTG ATGAGAAATG TTCCACTGAG CTCTTCGTAA 
     2041 GAGAGCCTCC AATTATGGTG ACCAAACAGC TGGAAGATAC AACTGCTTAT TGTGGGGAGA 
     2101 GAGTGGAATT AGAATGTGAG GTGTCTGAAG ATGATGCCAA TGTAAAATGG TTTAAGAATG 
     2161 GTGAAGAGAT TATCCCTGGT CCAAAATCAA GATACCGAAT TAGAGTTGAG GGTAAAAAAC 
     2221 ACATCTTGAT CATAGAGGGA GCAACAAAGG CTGATGCTGC AGAATATTCA GTAATGACAA 
     2281 CAGGAGGACA ATCATCTGCT AAACTTAGTG TTGACTTGAA ACCTCTGAAG ATTTTGACAC 
     2341 CTCTGACTGA TCAGACTGTA AATCTTGGAA AAGAAATCTG CCTGAAGTGT GAAATCTCTG 
     2401 AAAACATACC AGGAAAATGG ACTAAAAATG GCCTACCTGT TCAGGAGAGT GACCGTCTAA 
     2461 AGGTGGTTCA CAAGGGAAGG ATCCACAAGT TAGTGATAGC CAATGCCCTC ACTGAAGATG 
     2521 AAGGTGATTA TGTATTTGCA CCTGATGCCT ACAATGTTAC TCTGCCTGCC AAAGTTCATG 
     2581 TTATTGATCC TCCTAAGATC ATCCTGGATG GTCTTGATGC TGACAACACA GTGACAGTGA 
     2641 TTGCAGGAAA CAAGCTTCGT CTTGAGATCC CCATCAGCGG AGAACCACCT CCTAAAGCCA 
     2701 TGTGGAGCCG GGGAGATAAG GCTATTATGG AAGGCAGTGG CCGGATAAGA ACAGAATCTT 
     2761 ACCCTGATAG CAGCACTCTG GTCATTGATA TAGCTGAAAG AGATGACTCT GGTGTTTACC 
     2821 ACATCAATCT GAAAAACGAA GCTGGAGAGG CACATGCAAG CATCAAGGTT AAAGTTGTGG 
     2881 ACTTCCCTGA TCCTCCAGTG GCACCGACTG TGACAGAGGT GGGAGATGAC TGGTGTATCA 
     2941 TGAACTGGGA GCCTCCTGCC TACGACGGAG GCTCTCCAAT CCTAGGATAT TTTATTGAGA 
     3001 GGAAGAAGAA ACAAAGCTCC AGGTGGATGA GGCTGAATTT TGATCTCTGC AAAGAAACAA 
     3061 CTTTTGAGCC CAAGAAGATG ATTGAAGGTG TGGCCTATGA GGTCCGCATC TTTGCAGTCA 
     3121 ATGCCATTGG CATCTCCAAG CCCAGTATGC CCTCCAGGCC TTTTGTTCCT TTGGCTGTAA 
     3181 CAAGCCCTCC TACTCTTCTG ACTGTGGACT CTGTCACTGA CACGACTGTC ACGATGAGGT 
     3241 GGCGCCCCCC AGACCACATT GGTGCAGCAG GTTTAGATGG CTATGTGCTA GAGTATTGCT 
     3301 TTGAAGGAAG TACATCAGCA AAACAGTCTG ATGAAAATGG GGAGGCTGCC TATGATCTGC 
     3361 CAGCTGAGGA CTGGATAGTT GCAAACAAAG ATCTGATTGA CAAGACGAAG TTCACCATCA 
     3421 CAGGTCTGCC AACAGATGCA AAGATCTTTG TGCGTGTGAA GGCTGTTAAT GCAGCTGGTG 
     3481 CCAGCGAGCC CAAGTACTAT TCTCAGCCCA TTCTCGTGAA GGAAATCATA GAACCTCCAA 
     3541 AGATTCGCAT TCCAAGACAC CTGAAGCAAA CCTATATCCG CAGAGTTGGA GAAGCTGTCA 
     3601 ATCTGGTTAT ACCTTTCCAG GGAAAACCAA GACCAGAATT AACTTGGAAG AAGGATGGTG 
     3661 CAGAAATTGA TAAGAATCAA ATAAACATTC GCAACTCTGA GACTGATACA ATCATATTTA 
     3721 TTAGAAAAGC AGAGAGGAGC CACTCTGGGA AATATGATCT GCAAGTCAAA GTGGACAAAT 
     3781 TCGTGGAGAC CGCATCAATT GACATCCAGA TCATTGACCG TCCAGGTCCA CCCCAAATTG 
     3841 TGAAGATTGA GGATGTCTGG GGAGAAAATG TCGCTCTCAC ATGGACTCCA CCAAAGGATG 
     3901 ATGGAAATGC TGCTATCACA GGCTATACCA TTCAGAAGGC TGACAAGAAG AGCATGGAAT 
     3961 GGTTTACTGT CATTGAGCAT TATCATCGAA CCAGTGCCAC CATTACTGAA TTGGTCATAG 
     4021 GGAATGAATA TTACTTCCGG GTCTTTTCTG AAAACATGTG TGGCCTCAGT GAGGATGCCA 
     4081 CCATGACTAA AGAGAGTGCA GTGATCGCCA GGGATGGTAA AATCTACAAA AATCCAGTGT 
     4141 ATGAAGACTT TGATTTCTCA GAGGCACCCA TGTTTACTCA GCCTTTGGTT AACACCTATG 
     4201 CCATAGCTGG TTACAATGCC ACCCTAAACT GCAGTGTGAG AGGAAATCCT AAGCCTAAAA 
     4261 TAACCTGGAT GAAAAACAAA GTTGCTATTG TGGATGATCC AAGATACAGG ATGTTCAGCA 
     4321 ACCAGGGAGT CTGTACCCTG GAAATTCGCA AGCCCAGCCC CTATGATGGA GGCACTTACT 
     4381 GCTGCAAAGC AGTCAATGAC CTTGGGACAG TGGAGATTGA ATGCAAACTG GAGGTGAAAG 
     4441 TGATATATCA AGGAGTAAAT ACCCCTGGAC AACCAGTCTT CCTGGAGGGG CAGCAACAGG 
     4501 TGGGCTCTCC TTCTGCAGAC TCCTCTTGCA AGGCGTACCT CCAAACAACG CGTACGCGGC 
     4561 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     4621 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4681 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4741 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4801 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4861 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4921 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     4981 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     5041 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     5101 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     5161 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     5221 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     5281 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     5341 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     5401 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     5461 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     5521 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     5581 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     5641 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     5701 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     5761 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     5821 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     5881 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     5941 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     6001 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     6061 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     6121 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     6181 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     6241 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     6301 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     6361 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     6421 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     6481 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     6541 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     6601 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     6661 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     6721 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     6781 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     6841 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     6901 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     6961 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     7021 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     7081 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     7141 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     7201 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     7261 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     7321 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     7381 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     7441 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     7501 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     7561 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     7621 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     7681 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     7741 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     7801 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     7861 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     7921 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     7981 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     8041 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     8101 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     8161 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     8221 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     8281 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     8341 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//