LOCUS       RC235203                8385 bp    DNA     circular  SYN 
DEFINITION  ARHGAP20 (Myc-DDK tagged) - Homo sapiens Rho GTPase activating protein 20 (ARHGAP20), transcript variant 2
ACCESSION   NM_001258415
VERSION     NM_001258415.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4542..4633 
                     /label="Myc-DDK"  
     misc_feature    4663..5248 
                     /label="PolyA signal"  
     misc_feature    4720..4739 
                     /label="Restriction Enzyme"  
     misc_feature    5524..6196 
                     /label="ColE1"  
     misc_feature    6626..7420 
                     /label="Kan/Neo"  
     misc_feature    7455..7757 
                     /label="SV40 ori"  
     misc_feature    7843..8320 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4524..4530 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4524 
                     /label="ARHGAP20"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCTGCCCGG GAGCGGCAGC CGGCGCTGAA GAAGAAAATG AAAACACTAG 
     1081 CAGAAAGGAG GAGGAGCGCT CCATCTCTTA TCCTGGATAA AGCCCTACAA AAACGGCCTA 
     1141 CTACCAGGGA CAGTCCTTCT GCTAGTGTTG ACACATGCAC ATTTCTGTCA TCATTAGTGT 
     1201 GCTCCAATAG GACTCTGCTG ATTGATGGCC GGGCAGAACT CAAAAGAGGC CTCCAGAGGC 
     1261 AGGAGCGGCA TCTTTTCCTA TTCAATGATC TGTTTGTTGT GGCCAAAATC AAATATAACA 
     1321 ATAACTTTAA GATAAAAAAT AAAATTAAAT TAACTGATAT GTGGACAGCA AGCTGTGTGG 
     1381 ATGAAGTGGG AGAAGGCAAC ACCAATGCCA TGAAATCCTT TGTTTTGGGC TGGCCCACAG 
     1441 TGAACTTTGT GGCCACTTTC AGTTCTCCAG AACAAAAGGA CAAATGGCTC TCTCTCCTTC 
     1501 AGAGATACAT CAATCTAGAG AAAGAAAAGG ACTACCCGAA GAGCATTCCC CTCAAAATCT 
     1561 TCGCCAAGGA CATTGGGAAT TGTGCCTACT CTAAAACTAT AACAGTAATG AATTCAGATA 
     1621 CAGCGAATGA AGTTATCAAC ATGTCATTAC CAATGCTAGG GATAACTGGC TCTGAGAGAG 
     1681 ATTACCAGTT GTGGGTCAAT TCTGGCAAAG AAGAGGCTCC ATACCCACTC ATTGGGCATG 
     1741 AATATCCATA TGGAATTAAA ATGAGCCATC TTCGAGACTC TGCACTCCTG ACACCGGGAT 
     1801 CAAAGGACTC TACCACCCCT TTCAACCTCC AGGAGCCCTT CCTTATGGAA CAGCTCCCCC 
     1861 GAGAGATGCA GTGCCAGTTC ATCCTGAAGC CCAGCCGCCT GGCTGCAGCC CAGCAACTGA 
     1921 GTGATTCAGG TCATAAGACA TTTAAAAGGA GAAGATCTAT CATAAACTGG GCCTTCTGGC 
     1981 GAGGTTCTAG CACTCACCTG GACAACTTGC CCTCATCGCC AACATCACCT ATGCCAGGAC 
     2041 AGCTCTTTGG AATTTCTCTG CCAAATATTT GTGAGAATGA CAATCTGCCC AAACCTGTCT 
     2101 TGGATATGCT TTTCTTTCTT AATCAAAAAG GACCTCTCAC CAAAGGTATC TTCAGGCAAT 
     2161 CAGCCAATGT GAAATCCTGC AGAGAACTAA AAGAGAAATT GAATTCTGGA GTCGAAGTAC 
     2221 ACCTAGACTG TGAATCTATT TTTGTGATAG CATCTGTCTT AAAGGATTTT CTGCGAAATA 
     2281 TTCCAGGAAG TATTTTTTCA TCAGATCTCT ATGATCACTG GGTCTCTGTA ATGGATCAAG 
     2341 GAAATGATGA AGAGAAAATA AATACTGTTC AAAGGCTATT AGACCAGCTT CCGAGAGCCA 
     2401 ATGTTGTTCT CCTAAGGTAT CTTTTTGGGG TGTTACACAA CATTGAGCAA CATTCCTCAT 
     2461 CCAATCAGAT GACTGCATTT AATTTAGCTG TGTGTGTCGC TCCAAGTATT CTTTGGCCTC 
     2521 CTGCTTCCTC CAGCCCAGAA CTAGAAAACG AATTTACAAA AAAGGTTTCC CTGCTTATAC 
     2581 AATTTCTGAT TGAGAATTGC CTTAGGATAT TTGGAGAAGA AATCACTTCC CTCTTCAGAG 
     2641 AGGTTTCAGT GAGATGTGAC ACTAGAGAGA ATGCCTCAGA TATTTCTTGC TTTCAACTGA 
     2701 ATGACTCCTC CTATGACAGC TTGGAAAATG AGCTAAATGA GGATGTTGAT GCACCATGCA 
     2761 GTGACTTGGT AAAGAAACTT GGCCAGGGGA GCAGAAGCAT GGACTCTGTC TTAACCCTCA 
     2821 GTGACTATGA TCTTGACCAG CCCGAGGTGG AAGGCCTTTT AACCCTAAGC GACTTTGACT 
     2881 TGGCCCATTC TAAAGATGAA GATGTTCAAA TGAAACGGCC TCTTGAATCC AAGCCGGTGA 
     2941 ACATTTTAGT GTACACAAAG ATCCCACTGC GGGATCATGC CAGGGCCCCA TCTGCCATGT 
     3001 GCACACCCAG CTACCTGTCC ACAGCTGCAG CAAATGCTGC AAAAAGCCTG AGGCGACACC 
     3061 GGCGTTGCTC AGAGCCCAGC ATCGACTATC TGGATTCAAA GCTTTCCTAC CTCAGGGAGT 
     3121 TTTATCAGAA AAAGCTACGC AAGTCCAGCT GTGATGCAAT TCTTTCTCAA AAAGATGAAG 
     3181 ACTATCTGAA GCAGAATCAA CCCCTCCAGG AGGAAGGAAA GACATGTTTT AAACAGAGTT 
     3241 TAGTCACAGG CACTGATGTC AGCAAGAAAA ATGCCACTAC TCAAAACACT AAGAAGAAAA 
     3301 GCTTGTCTGG TAGTGAAGGA AATCACGTGA AACTTTTCCC TAAGTCTAAG CCAGTGGCCA 
     3361 TTTCTGTGGC ATCTTATAGT CCTATGTCCT CACAGGATCA TTCCAAGAAC CAGCCCTTTG 
     3421 ATGTGAATAC ATCTGGATAC TCCCCACCAC ACACAGCAGA TGCCCTCAAG GGTCCAAGGA 
     3481 CACATCGGCG CTGCTCAGAG CCCAACATAG AAGACCAGAA CCGCAAGCTG ACCTATCTCA 
     3541 GGGGAATTTA TTCAAAGAAA CAACATAAAA CCAGCTGTGA AGCTGGTCTC TTGCATGGAG 
     3601 AGGAGGATTA TCTCAAACGG CATAAGTCTT TGCAAATGGA GGGGCAGAAG CTCATTAATC 
     3661 AGAGTTTAGT CATGGGGATT GAGGTGGGCA AGAGTAGTGC CACAAACCAA AACACTGAGA 
     3721 AGGTTTTACC CCCAAGATTA AACCTTTGCC CAAGGACCAG CTATTCCAGC TTATCCTCCC 
     3781 CAGGCACTTC CCCATCCGGC TCATCAGTAA GCTCCCAAGA CAGTGCTTTT TCTCAGATTT 
     3841 CTGAACACTC TGTGTTTACA CCCACTGAGA CTTCCTCTCC AATAGATTGC ACTTTTCAGG 
     3901 CTCAGAGAAA ACGGGAAGAC CTTTCTCCTG ACTTTAGCAA TGCCAGCCAT GTTTCCGGAA 
     3961 TGCCCGGTCC CTCATCAGGG CAGGCTTGCA GCCGCCCAGC CTATACAAAG AAGGACACCA 
     4021 TGGAGTGGCA TTCACAAATG CATTCTGTAA CTCTTCATCC CAGCACATGG TTGAGAAATG 
     4081 GTGTGGCCAG TTTGAAAAAC TGGTCCCTCA AAAAGAAAGC AAAGGCAGCC AGACCAGAGG 
     4141 AAGAGAAAAT AGCTTCTCCA AAAGGACCCT TAGAGCCACC CCCACATGCT TCTGGTGTTC 
     4201 CAGAAGCCAA CTCACTGCAA GAGGAACAAA AAGACTTGCC CTTAAGGGCA GCTGAAGGAC 
     4261 TGTCCCCTGT GCAGTCAGCC CAAAGGTGTA GTTCTTCTCC CTTCCAGGAC TCAGAGAGAC 
     4321 ACTGTAGCTC TCCATTCAGC CTGGTGGAGA GCAGACTTAA GCTGTGCATG AAGTCACATG 
     4381 AGGAAATAGA GCCTGGTAGT CAGAGCTCTT CTGGTTCTCT GCCTTGGGAA AGAGCCTCAG 
     4441 CCAGCTCTTG GACTCTAGAG GATGCGACCA GCCCAGACTC AGGGCCTACA GTGGTCTGCG 
     4501 ACATTGAGGA CAGGTATTTA ACCAAAGACA TTACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     4561 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     4621 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4681 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     4741 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     4801 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     4861 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     4921 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     4981 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     5041 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     5101 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     5161 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5221 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5281 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     5341 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     5401 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     5461 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     5521 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     5581 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     5641 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     5701 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     5761 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     5821 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     5881 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     5941 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     6001 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     6061 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     6121 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     6181 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     6241 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     6301 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     6361 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     6421 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     6481 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     6541 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     6601 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     6661 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     6721 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     6781 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     6841 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     6901 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     6961 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     7021 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     7081 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     7141 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     7201 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     7261 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     7321 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     7381 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     7441 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     7501 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     7561 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     7621 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7681 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7741 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     7801 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     7861 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     7921 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     7981 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     8041 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     8101 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     8161 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     8221 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     8281 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     8341 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//