LOCUS       RC235201                8376 bp    DNA     circular  SYN 
DEFINITION  ARHGAP20 (Myc-DDK tagged) - Homo sapiens Rho GTPase activating protein 20 (ARHGAP20), transcript variant 3
ACCESSION   NM_001258416
VERSION     NM_001258416.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4533..4624 
                     /label="Myc-DDK"  
     misc_feature    4654..5239 
                     /label="PolyA signal"  
     misc_feature    4711..4730 
                     /label="Restriction Enzyme"  
     misc_feature    5515..6187 
                     /label="ColE1"  
     misc_feature    6617..7411 
                     /label="Kan/Neo"  
     misc_feature    7446..7748 
                     /label="SV40 ori"  
     misc_feature    7834..8311 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4515..4521 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4515 
                     /label="ARHGAP20"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACATTTTGG ATTATAATAA ATAAGAAAAT GAAAACACTA GCAGAAAGGA 
     1081 GGAGGAGCGC TCCATCTCTT ATCCTGGATA AAGCCCTACA AAAACGGCCT ACTACCAGGG 
     1141 ACAGTCCTTC TGCTAGTGTT GACACATGCA CATTTCTGTC ATCATTAGTG TGCTCCAATA 
     1201 GGACTCTGCT GATTGATGGC CGGGCAGAAC TCAAAAGAGG CCTCCAGAGG CAGGAGCGGC 
     1261 ATCTTTTCCT ATTCAATGAT CTGTTTGTTG TGGCCAAAAT CAAATATAAC AATAACTTTA 
     1321 AGATAAAAAA TAAAATTAAA TTAACTGATA TGTGGACAGC AAGCTGTGTG GATGAAGTGG 
     1381 GAGAAGGCAA CACCAATGCC ATGAAATCCT TTGTTTTGGG CTGGCCCACA GTGAACTTTG 
     1441 TGGCCACTTT CAGTTCTCCA GAACAAAAGG ACAAATGGCT CTCTCTCCTT CAGAGATACA 
     1501 TCAATCTAGA GAAAGAAAAG GACTACCCGA AGAGCATTCC CCTCAAAATC TTCGCCAAGG 
     1561 ACATTGGGAA TTGTGCCTAC TCTAAAACTA TAACAGTAAT GAATTCAGAT ACAGCGAATG 
     1621 AAGTTATCAA CATGTCATTA CCAATGCTAG GGATAACTGG CTCTGAGAGA GATTACCAGT 
     1681 TGTGGGTCAA TTCTGGCAAA GAAGAGGCTC CATACCCACT CATTGGGCAT GAATATCCAT 
     1741 ATGGAATTAA AATGAGCCAT CTTCGAGACT CTGCACTCCT GACACCGGGA TCAAAGGACT 
     1801 CTACCACCCC TTTCAACCTC CAGGAGCCCT TCCTTATGGA ACAGCTCCCC CGAGAGATGC 
     1861 AGTGCCAGTT CATCCTGAAG CCCAGCCGCC TGGCTGCAGC CCAGCAACTG AGTGATTCAG 
     1921 GTCATAAGAC ATTTAAAAGG AGAAGATCTA TCATAAACTG GGCCTTCTGG CGAGGTTCTA 
     1981 GCACTCACCT GGACAACTTG CCCTCATCGC CAACATCACC TATGCCAGGA CAGCTCTTTG 
     2041 GAATTTCTCT GCCAAATATT TGTGAGAATG ACAATCTGCC CAAACCTGTC TTGGATATGC 
     2101 TTTTCTTTCT TAATCAAAAA GGACCTCTCA CCAAAGGTAT CTTCAGGCAA TCAGCCAATG 
     2161 TGAAATCCTG CAGAGAACTA AAAGAGAAAT TGAATTCTGG AGTCGAAGTA CACCTAGACT 
     2221 GTGAATCTAT TTTTGTGATA GCATCTGTCT TAAAGGATTT TCTGCGAAAT ATTCCAGGAA 
     2281 GTATTTTTTC ATCAGATCTC TATGATCACT GGGTCTCTGT AATGGATCAA GGAAATGATG 
     2341 AAGAGAAAAT AAATACTGTT CAAAGGCTAT TAGACCAGCT TCCGAGAGCC AATGTTGTTC 
     2401 TCCTAAGGTA TCTTTTTGGG GTGTTACACA ACATTGAGCA ACATTCCTCA TCCAATCAGA 
     2461 TGACTGCATT TAATTTAGCT GTGTGTGTCG CTCCAAGTAT TCTTTGGCCT CCTGCTTCCT 
     2521 CCAGCCCAGA ACTAGAAAAC GAATTTACAA AAAAGGTTTC CCTGCTTATA CAATTTCTGA 
     2581 TTGAGAATTG CCTTAGGATA TTTGGAGAAG AAATCACTTC CCTCTTCAGA GAGGTTTCAG 
     2641 TGAGATGTGA CACTAGAGAG AATGCCTCAG ATATTTCTTG CTTTCAACTG AATGACTCCT 
     2701 CCTATGACAG CTTGGAAAAT GAGCTAAATG AGGATGTTGA TGCACCATGC AGTGACTTGG 
     2761 TAAAGAAACT TGGCCAGGGG AGCAGAAGCA TGGACTCTGT CTTAACCCTC AGTGACTATG 
     2821 ATCTTGACCA GCCCGAGGTG GAAGGCCTTT TAACCCTAAG CGACTTTGAC TTGGCCCATT 
     2881 CTAAAGATGA AGATGTTCAA ATGAAACGGC CTCTTGAATC CAAGCCGGTG AACATTTTAG 
     2941 TGTACACAAA GATCCCACTG CGGGATCATG CCAGGGCCCC ATCTGCCATG TGCACACCCA 
     3001 GCTACCTGTC CACAGCTGCA GCAAATGCTG CAAAAAGCCT GAGGCGACAC CGGCGTTGCT 
     3061 CAGAGCCCAG CATCGACTAT CTGGATTCAA AGCTTTCCTA CCTCAGGGAG TTTTATCAGA 
     3121 AAAAGCTACG CAAGTCCAGC TGTGATGCAA TTCTTTCTCA AAAAGATGAA GACTATCTGA 
     3181 AGCAGAATCA ACCCCTCCAG GAGGAAGGAA AGACATGTTT TAAACAGAGT TTAGTCACAG 
     3241 GCACTGATGT CAGCAAGAAA AATGCCACTA CTCAAAACAC TAAGAAGAAA AGCTTGTCTG 
     3301 GTAGTGAAGG AAATCACGTG AAACTTTTCC CTAAGTCTAA GCCAGTGGCC ATTTCTGTGG 
     3361 CATCTTATAG TCCTATGTCC TCACAGGATC ATTCCAAGAA CCAGCCCTTT GATGTGAATA 
     3421 CATCTGGATA CTCCCCACCA CACACAGCAG ATGCCCTCAA GGGTCCAAGG ACACATCGGC 
     3481 GCTGCTCAGA GCCCAACATA GAAGACCAGA ACCGCAAGCT GACCTATCTC AGGGGAATTT 
     3541 ATTCAAAGAA ACAACATAAA ACCAGCTGTG AAGCTGGTCT CTTGCATGGA GAGGAGGATT 
     3601 ATCTCAAACG GCATAAGTCT TTGCAAATGG AGGGGCAGAA GCTCATTAAT CAGAGTTTAG 
     3661 TCATGGGGAT TGAGGTGGGC AAGAGTAGTG CCACAAACCA AAACACTGAG AAGGTTTTAC 
     3721 CCCCAAGATT AAACCTTTGC CCAAGGACCA GCTATTCCAG CTTATCCTCC CCAGGCACTT 
     3781 CCCCATCCGG CTCATCAGTA AGCTCCCAAG ACAGTGCTTT TTCTCAGATT TCTGAACACT 
     3841 CTGTGTTTAC ACCCACTGAG ACTTCCTCTC CAATAGATTG CACTTTTCAG GCTCAGAGAA 
     3901 AACGGGAAGA CCTTTCTCCT GACTTTAGCA ATGCCAGCCA TGTTTCCGGA ATGCCCGGTC 
     3961 CCTCATCAGG GCAGGCTTGC AGCCGCCCAG CCTATACAAA GAAGGACACC ATGGAGTGGC 
     4021 ATTCACAAAT GCATTCTGTA ACTCTTCATC CCAGCACATG GTTGAGAAAT GGTGTGGCCA 
     4081 GTTTGAAAAA CTGGTCCCTC AAAAAGAAAG CAAAGGCAGC CAGACCAGAG GAAGAGAAAA 
     4141 TAGCTTCTCC AAAAGGACCC TTAGAGCCAC CCCCACATGC TTCTGGTGTT CCAGAAGCCA 
     4201 ACTCACTGCA AGAGGAACAA AAAGACTTGC CCTTAAGGGC AGCTGAAGGA CTGTCCCCTG 
     4261 TGCAGTCAGC CCAAAGGTGT AGTTCTTCTC CCTTCCAGGA CTCAGAGAGA CACTGTAGCT 
     4321 CTCCATTCAG CCTGGTGGAG AGCAGACTTA AGCTGTGCAT GAAGTCACAT GAGGAAATAG 
     4381 AGCCTGGTAG TCAGAGCTCT TCTGGTTCTC TGCCTTGGGA AAGAGCCTCA GCCAGCTCTT 
     4441 GGACTCTAGA GGATGCGACC AGCCCAGACT CAGGGCCTAC AGTGGTCTGC GACATTGAGG 
     4501 ACAGGTATTT AACCAAAGAC ATTACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4561 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4621 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4681 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4741 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4801 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4861 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     4921 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     4981 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     5041 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     5101 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     5161 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5221 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5281 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     5341 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     5401 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     5461 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5521 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     5581 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5641 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     5701 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     5761 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     5821 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     5881 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     5941 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     6001 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     6061 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     6121 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     6181 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     6241 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     6301 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     6361 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6421 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6481 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6541 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6601 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6661 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     6721 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     6781 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     6841 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     6901 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     6961 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     7021 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     7081 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     7141 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     7201 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     7261 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     7321 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     7381 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7441 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7501 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7561 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7621 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7681 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7741 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     7801 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     7861 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     7921 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     7981 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     8041 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     8101 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     8161 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     8221 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     8281 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     8341 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//