LOCUS       RC235196                8349 bp    DNA     circular  SYN 
DEFINITION  PPFIA2 (Myc-DDK tagged) - Homo sapiens protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 2 (PPFIA2), transcript variant 6
ACCESSION   NM_001220477
VERSION     NM_001220477.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4506..4597 
                     /label="Myc-DDK"  
     misc_feature    4627..5212 
                     /label="PolyA signal"  
     misc_feature    4684..4703 
                     /label="Restriction Enzyme"  
     misc_feature    5488..6160 
                     /label="ColE1"  
     misc_feature    6590..7384 
                     /label="Kan/Neo"  
     misc_feature    7419..7721 
                     /label="SV40 ori"  
     misc_feature    7807..8284 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4488..4494 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4488 
                     /label="PPFIA2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATTTTCTCG GATATGAACA CCGTTTCTGG CTCCCCTAAA GTGCATCCTC 
     1081 CTAATGGGAC CCGGTTTTAC ACTTTTCAAG AATTTGCTGC ACTGACAAAA GAATTAAATG 
     1141 CCTGCAGGGA ACAACTTCTA GAAAAGGAAG AAGAAATCTC TGAACTTAAA GCTGAAAGAA 
     1201 ACAACACAAG ACTATTACTG GAGCATTTGG AGTGCCTTGT GTCACGACAT GAAAGATCAC 
     1261 TAAGAATGAC GGTGGTAAAA CGGCAAGCCC AGTCTCCCTC AGGAGTATCC AGTGAAGTTG 
     1321 AAGTTCTCAA GGCACTGAAA TCTTTGTTTG AGCACCACAA GGCCTTGGAT GAAAAGGTAA 
     1381 GGGAGCGACT GAGGGTTTCT TTAGAAAGAG TCTCTGCACT GGAAGAAGAA CTAGCTGCTG 
     1441 CTAATCAGGA GATTGTTGCC TTGCGTGAAC AAAATGTTCA TATACAAAGA AAAATGGCAT 
     1501 CAAGCGAGGG ATCCACAGAG TCAGAACATC TTGAAGGGAT GGAACCTGGA CAGAAAGTCC 
     1561 ATGAGAAGCG TTTGTCCAAT GGTTCTATAG ACTCAACCGA TGAAACTAGT CAAATAGTTG 
     1621 AACTACAAGA ATTGCTTGAA AAGCAAAACT ATGAAATGGC CCAGATGAAA GAACGTTTAG 
     1681 CAGCCCTTTC TTCCCGAGTG GGAGAGGTGG AACAGGAAGC AGAGACAGCA AGAAAGGATC 
     1741 TCATTAAAAC AGAAGAAATG AACACCAAGT ATCAAAGGGA CATTAGGGAG GCCATGGCAC 
     1801 AAAAGGAAGA TATGGAAGAA AGAATTACAA CCCTTGAAAA GCGTTACCTC AGTGCTCAGA 
     1861 GAGAATCTAC CTCCATACAT GACATGAATG ATAAACTAGA AAATGAGTTA GCAAATAAAG 
     1921 AAGCTATCCT GCGGCAGATG GAAGAGAAAA ACAGACAGTT ACAAGAACGT CTTGAGCTAG 
     1981 CTGAACAAAA GTTGCAGCAG ACCATGAGAA AGGCTGAAAC CTTGCCTGAA GTAGAGGCTG 
     2041 AACTGGCTCA GAGAATTGCA GCCCTAACCA AGGCTGAAGA GAGACATGGA AATATTGAAG 
     2101 AACGTATGAG ACATTTAGAG GGTCAACTTG AAGAGAAGAA TCAAGAACTT CAAAGAGCTA 
     2161 GGCAAAGAGA GAAAATGAAT GAGGAGCATA ACAAGAGATT ATCGGATACG GTTGATAGAC 
     2221 TTCTGACTGA ATCCAATGAA CGCCTACAAC TACACTTAAA GGAAAGAATG GCTGCTCTAG 
     2281 AAGAAAAGAA TGTTTTAATT CAAGAATCAG AAACTTTCAG AAAGAATCTT GAAGAATCTT 
     2341 TACATGATAA GGAAAGATTA GCAGAAGAAA TTGAAAAGCT GAGATCTGAA CTTGACCAAT 
     2401 TGAAAATGAG AACTGGCTCT TTAATTGAAC CCACAATACC AAGAACTCAT CTAGACACCT 
     2461 CAGCTGAGTT GCGGTACTCA GTGGGATCCC TAGTGGACAG CCAGTCTGAT TACAGAACAA 
     2521 CTAAAGTAAT AAGAAGACCA AGGAGAGGCC GCATGGGTGT GCGAAGAGAT GAGCCAAAGG 
     2581 TGAAATCTCT TGGGGATCAC GAGTGGAATA GAACTCAACA GATTGGAGTA CTAAGCAGCC 
     2641 ACCCTTTTGA AAGTGACACT GAAATGTCTG ATATTGATGA TGATGACAGA GAAACAATTT 
     2701 TTAGCTCAAT GGATCTTCTC TCTCCAAGTG GTCATTCCGA TGCCCAGACG CTAGCCATGA 
     2761 TGCTTCAGGA ACAATTGGAT GCCATCAACA AAGAAATCAG GCTAATTCAG GAAGAAAAAG 
     2821 AATCTACAGA GTTGCGTGCT GAAGAAATTG AAAATAGAGT GGCTAGTGTG AGCCTCGAAG 
     2881 GCCTGAATTT GGCAAGGGTC CACCCAGGTA CCTCCATTAC TGCCTCTGTT ACAGCTTCAT 
     2941 CGCTGGCCAG TTCATCTCCC CCCAGTGGAC ACTCAACTCC AAAGCTCACC CCTCGAAGCC 
     3001 CTGCCAGGGA AATGGATCGG ATGGGAGTCA TGACACTGCC AAGTGATCTG AGGAAACATC 
     3061 GGAGAAAGAT TGCAGTTGTG GAAGAAGATG GTCGAGAGGA CAAAGCAACA ATTAAATGTG 
     3121 AAACTTCTCC TCCTCCTACC CCTAGAGCCC TCAGAATGAC TCACACTCTC CCTTCTTCCT 
     3181 ACCACAATGA TGCTCGAAGT AGTTTATCTG TCTCTCTTGA GCCAGAAAGC CTCGGGCTTG 
     3241 GTAGTGCCAA CAGCAGCCAA GACTCTCTTC ACAAAGCCCC CAAGAAGAAA GGAATCAAGT 
     3301 CTTCAATAGG ACGTTTGTTT GGTAAAAAAG AAAAAGCTCG ACTTGGGCAG CTCCGAGGCT 
     3361 TTATGGAGAC TGAAGCTGCA GCTCAGGAGT CCCTGGGGTT AGGCAAACTC GGAACTCAAG 
     3421 CTGAGAAGGA TCGAAGACTA AAGAAAAAGC ATGAACTTCT TGAAGAAGCT CGGAGAAAGG 
     3481 GATTACCTTT TGCCCAGTGG GATGGGCCAA CTGTGGTCGC ATGGCTAGAG CTTTGGTTGG 
     3541 GAATGCCTGC GTGGTACGTG GCAGCCTGCC GAGCCAACGT GAAGAGTGGT GCCATCATGT 
     3601 CTGCTTTATC TGACACTGAG ATCCAGAGAG AAATTGGAAT CAGCAATCCA CTGCATCGCT 
     3661 TAAAACTTCG ATTAGCAATC CAGGAGATGG TTTCCCTAAC AAGTCCTTCA GCTCCTCCAA 
     3721 CATCTCGAAC TAAAGAATCT GAGGAAGGAA GCTGGGCCCA GACCCTGGCT TATGGAGATA 
     3781 TGAATCATGA GTGGATTGGA AATGAATGGC TTCCCAGCTT GGGGTTACCT CAGTACAGAA 
     3841 GTTACTTTAT GGAATGCTTG GTAGATGCAA GAATGTTAGA TCACCTAACA AAAAAAGATC 
     3901 TCCGTGTCCA TTTAAAAATG GTGGATAGTT TCCATCGAAC AAGTTTACAA TATGGAATTA 
     3961 TGTGCTTAAA GAGGTTGAAT TATGACAGAA AAGAACTAGA AAGAAGACGG GAAGCAAGCC 
     4021 AACATGAAAT AAAAGACGTG TTGGTGTGGA GCAATGACCG AGTTATTCGC TGGATACAAG 
     4081 CAATTGGACT TCGAGAATAT GCAAATAATA TACTTGAGAG CGGTGTGCAT GGCTCACTTA 
     4141 TAGCCCTGGA TGAAAACTTT GACTACAGCA GCTTAGCTTT ATTATTACAG ATTCCAACAC 
     4201 AGAACACCCA GGCAAGGCAG ATTCTTGAAA GAGAATACAA TAACCTCTTG GCCCTGGGAA 
     4261 CTGAAAGGCG ACTGGATGAA AGTGATGACA AGAACTTCAG ACGTGGATCA ACCTGGAGAA 
     4321 GGCAGTTTCC TCCTCGTGAA GTACATGGAA TCAGCATGAT GCCTGGGTCC TCAGAAACAT 
     4381 TACCAGCTGG ATTTAGGTTA ACCACAACCT CTGGGCAGTC AAGAAAAATG ACAACAGATG 
     4441 TTGCTTCATC AAGACTGCAG AGGTTAGACA ACTCCACTGT TCGCACATAC TCATGTACGC 
     4501 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4561 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4621 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4681 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4741 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4801 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     4861 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     4921 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     4981 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5041 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5101 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5161 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5221 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5281 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5341 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5401 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5461 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5521 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5581 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     5641 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     5701 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     5761 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     5821 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     5881 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     5941 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6001 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6061 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6121 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6181 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6241 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6301 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6361 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6421 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6481 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6541 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6601 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     6661 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     6721 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     6781 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     6841 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     6901 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     6961 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7021 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7081 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7141 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7201 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7261 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7321 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7381 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7441 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7501 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7561 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7621 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     7681 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7741 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     7801 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     7861 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     7921 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     7981 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     8041 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8101 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8161 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8221 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8281 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8341 GCGAATTTT
//