LOCUS       RC235194                8346 bp    DNA     circular  SYN 
DEFINITION  ARHGAP20 (Myc-DDK tagged) - Homo sapiens Rho GTPase activating protein 20 (ARHGAP20), transcript variant 5
ACCESSION   NM_001258418
VERSION     NM_001258418.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4503..4594 
                     /label="Myc-DDK"  
     misc_feature    4624..5209 
                     /label="PolyA signal"  
     misc_feature    4681..4700 
                     /label="Restriction Enzyme"  
     misc_feature    5485..6157 
                     /label="ColE1"  
     misc_feature    6587..7381 
                     /label="Kan/Neo"  
     misc_feature    7416..7718 
                     /label="SV40 ori"  
     misc_feature    7804..8281 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4485..4491 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4485 
                     /label="ARHGAP20"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAAACACTA GCAGAAAGGA GGAGGAGCGC TCCATCTCTT ATCCTGGATA 
     1081 AAGCCCTACA AAAACGGCCT ACTACCAGGG ACAGTCCTTC TGCTAGTGTT GACACATGCA 
     1141 CATTTCTGTC ATCATTAGTG TGCTCCAATA GGACTCTGCT GATTGATGGC CGGGCAGAAC 
     1201 TCAAAAGAGG CCTCCAGAGG CAGGAGCGGC ATCTTTTCCT ATTCAATGAT CTGTTTGTTG 
     1261 TGGCCAAAAT CAAATATAAC AATAACTTTA AGATAAAAAA TAAAATTAAA TTAACTGATA 
     1321 TGTGGACAGC AAGCTGTGTG GATGAAGTGG GAGAAGGCAA CACCAATGCC ATGAAATCCT 
     1381 TTGTTTTGGG CTGGCCCACA GTGAACTTTG TGGCCACTTT CAGTTCTCCA GAACAAAAGG 
     1441 ACAAATGGCT CTCTCTCCTT CAGAGATACA TCAATCTAGA GAAAGAAAAG GACTACCCGA 
     1501 AGAGCATTCC CCTCAAAATC TTCGCCAAGG ACATTGGGAA TTGTGCCTAC TCTAAAACTA 
     1561 TAACAGTAAT GAATTCAGAT ACAGCGAATG AAGTTATCAA CATGTCATTA CCAATGCTAG 
     1621 GGATAACTGG CTCTGAGAGA GATTACCAGT TGTGGGTCAA TTCTGGCAAA GAAGAGGCTC 
     1681 CATACCCACT CATTGGGCAT GAATATCCAT ATGGAATTAA AATGAGCCAT CTTCGAGACT 
     1741 CTGCACTCCT GACACCGGGA TCAAAGGACT CTACCACCCC TTTCAACCTC CAGGAGCCCT 
     1801 TCCTTATGGA ACAGCTCCCC CGAGAGATGC AGTGCCAGTT CATCCTGAAG CCCAGCCGCC 
     1861 TGGCTGCAGC CCAGCAACTG AGTGATTCAG GTCATAAGAC ATTTAAAAGG AGAAGATCTA 
     1921 TCATAAACTG GGCCTTCTGG CGAGGTTCTA GCACTCACCT GGACAACTTG CCCTCATCGC 
     1981 CAACATCACC TATGCCAGGA CAGCTCTTTG GAATTTCTCT GCCAAATATT TGTGAGAATG 
     2041 ACAATCTGCC CAAACCTGTC TTGGATATGC TTTTCTTTCT TAATCAAAAA GGACCTCTCA 
     2101 CCAAAGGTAT CTTCAGGCAA TCAGCCAATG TGAAATCCTG CAGAGAACTA AAAGAGAAAT 
     2161 TGAATTCTGG AGTCGAAGTA CACCTAGACT GTGAATCTAT TTTTGTGATA GCATCTGTCT 
     2221 TAAAGGATTT TCTGCGAAAT ATTCCAGGAA GTATTTTTTC ATCAGATCTC TATGATCACT 
     2281 GGGTCTCTGT AATGGATCAA GGAAATGATG AAGAGAAAAT AAATACTGTT CAAAGGCTAT 
     2341 TAGACCAGCT TCCGAGAGCC AATGTTGTTC TCCTAAGGTA TCTTTTTGGG GTGTTACACA 
     2401 ACATTGAGCA ACATTCCTCA TCCAATCAGA TGACTGCATT TAATTTAGCT GTGTGTGTCG 
     2461 CTCCAAGTAT TCTTTGGCCT CCTGCTTCCT CCAGCCCAGA ACTAGAAAAC GAATTTACAA 
     2521 AAAAGGTTTC CCTGCTTATA CAATTTCTGA TTGAGAATTG CCTTAGGATA TTTGGAGAAG 
     2581 AAATCACTTC CCTCTTCAGA GAGGTTTCAG TGAGATGTGA CACTAGAGAG AATGCCTCAG 
     2641 ATATTTCTTG CTTTCAACTG AATGACTCCT CCTATGACAG CTTGGAAAAT GAGCTAAATG 
     2701 AGGATGTTGA TGCACCATGC AGTGACTTGG TAAAGAAACT TGGCCAGGGG AGCAGAAGCA 
     2761 TGGACTCTGT CTTAACCCTC AGTGACTATG ATCTTGACCA GCCCGAGGTG GAAGGCCTTT 
     2821 TAACCCTAAG CGACTTTGAC TTGGCCCATT CTAAAGATGA AGATGTTCAA ATGAAACGGC 
     2881 CTCTTGAATC CAAGCCGGTG AACATTTTAG TGTACACAAA GATCCCACTG CGGGATCATG 
     2941 CCAGGGCCCC ATCTGCCATG TGCACACCCA GCTACCTGTC CACAGCTGCA GCAAATGCTG 
     3001 CAAAAAGCCT GAGGCGACAC CGGCGTTGCT CAGAGCCCAG CATCGACTAT CTGGATTCAA 
     3061 AGCTTTCCTA CCTCAGGGAG TTTTATCAGA AAAAGCTACG CAAGTCCAGC TGTGATGCAA 
     3121 TTCTTTCTCA AAAAGATGAA GACTATCTGA AGCAGAATCA ACCCCTCCAG GAGGAAGGAA 
     3181 AGACATGTTT TAAACAGAGT TTAGTCACAG GCACTGATGT CAGCAAGAAA AATGCCACTA 
     3241 CTCAAAACAC TAAGAAGAAA AGCTTGTCTG GTAGTGAAGG AAATCACGTG AAACTTTTCC 
     3301 CTAAGTCTAA GCCAGTGGCC ATTTCTGTGG CATCTTATAG TCCTATGTCC TCACAGGATC 
     3361 ATTCCAAGAA CCAGCCCTTT GATGTGAATA CATCTGGATA CTCCCCACCA CACACAGCAG 
     3421 ATGCCCTCAA GGGTCCAAGG ACACATCGGC GCTGCTCAGA GCCCAACATA GAAGACCAGA 
     3481 ACCGCAAGCT GACCTATCTC AGGGGAATTT ATTCAAAGAA ACAACATAAA ACCAGCTGTG 
     3541 AAGCTGGTCT CTTGCATGGA GAGGAGGATT ATCTCAAACG GCATAAGTCT TTGCAAATGG 
     3601 AGGGGCAGAA GCTCATTAAT CAGAGTTTAG TCATGGGGAT TGAGGTGGGC AAGAGTAGTG 
     3661 CCACAAACCA AAACACTGAG AAGGTTTTAC CCCCAAGATT AAACCTTTGC CCAAGGACCA 
     3721 GCTATTCCAG CTTATCCTCC CCAGGCACTT CCCCATCCGG CTCATCAGTA AGCTCCCAAG 
     3781 ACAGTGCTTT TTCTCAGATT TCTGAACACT CTGTGTTTAC ACCCACTGAG ACTTCCTCTC 
     3841 CAATAGATTG CACTTTTCAG GCTCAGAGAA AACGGGAAGA CCTTTCTCCT GACTTTAGCA 
     3901 ATGCCAGCCA TGTTTCCGGA ATGCCCGGTC CCTCATCAGG GCAGGCTTGC AGCCGCCCAG 
     3961 CCTATACAAA GAAGGACACC ATGGAGTGGC ATTCACAAAT GCATTCTGTA ACTCTTCATC 
     4021 CCAGCACATG GTTGAGAAAT GGTGTGGCCA GTTTGAAAAA CTGGTCCCTC AAAAAGAAAG 
     4081 CAAAGGCAGC CAGACCAGAG GAAGAGAAAA TAGCTTCTCC AAAAGGACCC TTAGAGCCAC 
     4141 CCCCACATGC TTCTGGTGTT CCAGAAGCCA ACTCACTGCA AGAGGAACAA AAAGACTTGC 
     4201 CCTTAAGGGC AGCTGAAGGA CTGTCCCCTG TGCAGTCAGC CCAAAGGTGT AGTTCTTCTC 
     4261 CCTTCCAGGA CTCAGAGAGA CACTGTAGCT CTCCATTCAG CCTGGTGGAG AGCAGACTTA 
     4321 AGCTGTGCAT GAAGTCACAT GAGGAAATAG AGCCTGGTAG TCAGAGCTCT TCTGGTTCTC 
     4381 TGCCTTGGGA AAGAGCCTCA GCCAGCTCTT GGACTCTAGA GGATGCGACC AGCCCAGACT 
     4441 CAGGGCCTAC AGTGGTCTGC GACATTGAGG ACAGGTATTT AACCAAAGAC ATTACGCGTA 
     4501 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     4561 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4621 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4681 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     4741 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     4801 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     4861 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     4921 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     4981 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     5041 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     5101 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     5161 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5221 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     5281 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     5341 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     5401 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     5461 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     5521 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     5581 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     5641 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     5701 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     5761 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     5821 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     5881 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     5941 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     6001 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     6061 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     6121 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     6181 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     6241 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     6301 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     6361 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     6421 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     6481 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     6541 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     6601 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     6661 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     6721 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     6781 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     6841 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     6901 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     6961 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     7021 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     7081 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     7141 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     7201 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     7261 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     7321 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     7381 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     7441 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     7501 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     7561 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     7621 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     7681 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7741 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     7801 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     7861 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     7921 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     7981 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     8041 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     8101 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     8161 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     8221 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     8281 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     8341 AATTTT
//