LOCUS       RC235191                8337 bp    DNA     circular  SYN 
DEFINITION  PPFIA2 (Myc-DDK tagged) - Homo sapiens protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 2 (PPFIA2), transcript variant 7
ACCESSION   NM_001220478
VERSION     NM_001220478.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4494..4585 
                     /label="Myc-DDK"  
     misc_feature    4615..5200 
                     /label="PolyA signal"  
     misc_feature    4672..4691 
                     /label="Restriction Enzyme"  
     misc_feature    5476..6148 
                     /label="ColE1"  
     misc_feature    6578..7372 
                     /label="Kan/Neo"  
     misc_feature    7407..7709 
                     /label="SV40 ori"  
     misc_feature    7795..8272 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4476..4482 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4476 
                     /label="PPFIA2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATTTTCTCG GATATGAACA CCGTTTCTGG CTCCCCTAAA GTGCATCCTC 
     1081 CTAATGGGAC CCGGTTTTAC ACTTTTCAAG AATTTGCTGC ACTGACAAAA GAATTAAATG 
     1141 CCTGCAGGGA ACAACTTCTA GAAAAGGAAG AAGAAATCTC TGAACTTAAA GCTGAAAGAA 
     1201 ACAACACAAG ACTATTACTG GAGCATTTGG AGTGCCTTGT GTCACGACAT GAAAGATCAC 
     1261 TAAGAATGAC GGTGGTAAAA CGGCAAGCCC AGTCTCCCTC AGGAGTATCC AGTGAAGTTG 
     1321 AAGTTCTCAA GGCACTGAAA TCTTTGTTTG AGCACCACAA GGCCTTGGAT GAAAAGATTG 
     1381 TTGCCTTGCG TGAACAAAAT GTTCATATAC AAAGAAAAAT GGCATCAAGC GAGGGATCCA 
     1441 CAGAGTCAGA ACATCTTGAA GGGATGGAAC CTGGACAGAA AGTCCATGAG AAGCGTTTGT 
     1501 CCAATGGTTC TATAGACTCA ACCGATGAAA CTAGTCAAAT AGTTGAACTA CAAGAATTGC 
     1561 TTGAAAAGCA AAACTATGAA ATGGCCCAGA TGAAAGAACG TTTAGCAGCC CTTTCTTCCC 
     1621 GAGTGGGAGA GGTGGAACAG GAAGCAGAGA CAGCAAGAAA GGATCTCATT AAAACAGAAG 
     1681 AAATGAACAC CAAGTATCAA AGGGACATTA GGGAGGCCAT GGCACAAAAG GAAGATATGG 
     1741 AAGAAAGAAT TACAACCCTT GAAAAGCGTT ACCTCAGTGC TCAGAGAGAA TCTACCTCCA 
     1801 TACATGACAT GAATGATAAA CTAGAAAATG AGTTAGCAAA TAAAGAAGCT ATCCTGCGGC 
     1861 AGATGGAAGA GAAAAACAGA CAGTTACAAG AACGTCTTGA GCTAGCTGAA CAAAAGTTGC 
     1921 AGCAGACCAT GAGAAAGGCT GAAACCTTGC CTGAAGTAGA GGCTGAACTG GCTCAGAGAA 
     1981 TTGCAGCCCT AACCAAGGCT GAAGAGAGAC ATGGAAATAT TGAAGAACGT ATGAGACATT 
     2041 TAGAGGGTCA ACTTGAAGAG AAGAATCAAG AACTTCAAAG AGCTAGGCAA AGAGAGAAAA 
     2101 TGAATGAGGA GCATAACAAG AGATTATCGG ATACGGTTGA TAGACTTCTG ACTGAATCCA 
     2161 ATGAACGCCT ACAACTACAC TTAAAGGAAA GAATGGCTGC TCTAGAAGAA AAGAATGTTT 
     2221 TAATTCAAGA ATCAGAAACT TTCAGAAAGA ATCTTGAAGA ATCTTTACAT GATAAGGAAA 
     2281 GATTAGCAGA AGAAATTGAA AAGCTGAGAT CTGAACTTGA CCAATTGAAA ATGAGAACTG 
     2341 GCTCTTTAAT TGAACCCACA ATACCAAGAA CTCATCTAGA CACCTCAGCT GAGTTGCGGT 
     2401 ACTCAGTGGG ATCCCTAGTG GACAGCCAGT CTGATTACAG AACAACTAAA GTAATAAGAA 
     2461 GACCAAGGAG AGGCCGCATG GGTGTGCGAA GAGATGAGCC AAAGGTGAAA TCTCTTGGGG 
     2521 ATCACGAGTG GAATAGAACT CAACAGATTG GAGTACTAAG CAGCCACCCT TTTGAAAGTG 
     2581 ACACTGAAAT GTCTGATATT GATGATGATG ACAGAGAAAC AATTTTTAGC TCAATGGATC 
     2641 TTCTCTCTCC AAGTGGTCAT TCCGATGCCC AGACGCTAGC CATGATGCTT CAGGAACAAT 
     2701 TGGATGCCAT CAACAAAGAA ATCAGGCTAA TTCAGGAAGA AAAAGAATCT ACAGAGTTGC 
     2761 GTGCTGAAGA AATTGAAAAT AGAGTGGCTA GTGTGAGCCT CGAAGGCCTG AATTTGGCAA 
     2821 GGGTCCACCC AGGTACCTCC ATTACTGCCT CTGTTACAGC TTCATCGCTG GCCAGTTCAT 
     2881 CTCCCCCCAG TGGACACTCA ACTCCAAAGC TCACCCCTCG AAGCCCTGCC AGGGAAATGG 
     2941 ATCGGATGGG AGTCATGACA CTGCCAAGTG ATCTGAGGAA ACATCGGAGA AAGATTGCAG 
     3001 TTGTGGAAGA AGATGGTCGA GAGGACAAAG CAACAATTAA ATGTGAAACT TCTCCTCCTC 
     3061 CTACCCCTAG AGCCCTCAGA ATGACTCACA CTCTCCCTTC TTCCTACCAC AATGATGCTC 
     3121 GAAGTAGTTT ATCTGTCTCT CTTGAGCCAG AAAGCCTCGG GCTTGGTAGT GCCAACAGCA 
     3181 GCCAAGACTC TCTTCACAAA GCCCCCAAGA AGAAAGGAAT CAAGTCTTCA ATAGGACGTT 
     3241 TGTTTGGTAA AAAAGAAAAA GCTCGACTTG GGCAGCTCCG AGGCTTTATG GAGACTGAAG 
     3301 CTGCAGCTCA GGAGTCCCTG GGGTTAGGCA AACTCGGAAC TCAAGCTGAG AAGGATCGAA 
     3361 GACTAAAGAA AAAGCATGAA CTTCTTGAAG AAGCTCGGAG AAAGGGATTA CCTTTTGCCC 
     3421 AGTGGGATGG GCCAACTGTG GTCGCATGGC TAGAGCTTTG GTTGGGAATG CCTGCGTGGT 
     3481 ACGTGGCAGC CTGCCGAGCC AACGTGAAGA GTGGTGCCAT CATGTCTGCT TTATCTGACA 
     3541 CTGAGATCCA GAGAGAAATT GGAATCAGCA ATCCACTGCA TCGCTTAAAA CTTCGATTAG 
     3601 CAATCCAGGA GATGGTTTCC CTAACAAGTC CTTCAGCTCC TCCAACATCT CGAACTCCTT 
     3661 CAGGCAACGT TTGGGTGACT CATGAAGAAA TGGAAAATCT TGCAGCTCCA GCAAAAACGA 
     3721 AAGAATCTGA GGAAGGAAGC TGGGCCCAGA CCCTGGCTTA TGGAGATATG AATCATGAGT 
     3781 GGATTGGAAA TGAATGGCTT CCCAGCTTGG GGTTACCTCA GTACAGAAGT TACTTTATGG 
     3841 AATGCTTGGT AGATGCAAGA ATGTTAGATC ACCTAACAAA AAAAGATCTC CGTGTCCATT 
     3901 TAAAAATGGT GGATAGTTTC CATCGAACAA GTTTACAATA TGGAATTATG TGCTTAAAGA 
     3961 GGTTGAATTA TGACAGAAAA GAACTAGAAA GAAGACGGGA AGCAAGCCAA CATGAAATAA 
     4021 AAGACGTGTT GGTGTGGAGC AATGACCGAG TTATTCGCTG GATACAAGCA ATTGGACTTC 
     4081 GAGAATATGC AAATAATATA CTTGAGAGCG GTGTGCATGG CTCACTTATA GCCCTGGATG 
     4141 AAAACTTTGA CTACAGCAGC TTAGCTTTAT TATTACAGAT TCCAACACAG AACACCCAGG 
     4201 CAAGGCAGAT TCTTGAAAGA GAATACAATA ACCTCTTGGC CCTGGGAACT GAAAGGCGAC 
     4261 TGGATGAAAG TGATGACAAG AACTTCAGAC GTGGATCAAC CTGGAGAAGG CAGTTTCCTC 
     4321 CTCGTGAAGT ACATGGAATC AGCATGATGC CTGGGTCCTC AGAAACATTA CCAGCTGGAT 
     4381 TTAGGTTAAC CACAACCTCT GGGCAGTCAA GAAAAATGAC AACAGATGTT GCTTCATCAA 
     4441 GACTGCAGAG GTTAGACAAC TCCACTGTTC GCACATACTC ATGTACGCGT ACGCGGCCGC 
     4501 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     4561 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4621 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4681 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4741 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4801 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4861 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     4921 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     4981 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     5041 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     5101 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     5161 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5221 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     5281 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5341 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5401 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     5461 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     5521 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     5581 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     5641 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     5701 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     5761 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     5821 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     5881 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     5941 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     6001 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     6061 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     6121 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     6181 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     6241 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6301 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6361 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6421 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     6481 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     6541 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     6601 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     6661 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     6721 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     6781 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     6841 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     6901 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     6961 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     7021 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     7081 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     7141 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     7201 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     7261 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7321 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7381 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7441 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     7501 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     7561 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7621 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     7681 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     7741 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     7801 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     7861 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     7921 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     7981 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     8041 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     8101 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     8161 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     8221 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     8281 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//