LOCUS       RC235190                8334 bp    DNA     circular  SYN 
DEFINITION  SEMA5B (Myc-DDK tagged) - Homo sapiens sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B (SEMA5B), transcript variant 2
ACCESSION   NM_001256346
VERSION     NM_001256346.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4491..4582 
                     /label="Myc-DDK"  
     misc_feature    4612..5197 
                     /label="PolyA signal"  
     misc_feature    4669..4688 
                     /label="Restriction Enzyme"  
     misc_feature    5473..6145 
                     /label="ColE1"  
     misc_feature    6575..7369 
                     /label="Kan/Neo"  
     misc_feature    7404..7706 
                     /label="SV40 ori"  
     misc_feature    7792..8269 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4473..4479 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4473 
                     /label="SEMA5B"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCCTGTGGC TTCAGTCCGT CTCCTGTTGC CCACCACCTC GTCCCTGGGC 
     1081 CGCCTGATAC CCCAGCCCAA CAGCTAAGGT GTGGATGGAC AGTAGGGGGC TGGCTTCTCT 
     1141 CACTGGTCAG GGGTCTTCTC CCCTGTCTGC CTCCCGGAGC TAGGACTGCA GAGGGGCCTA 
     1201 TCATGGTGCT TGCAGGCCCC CTGGCTGTCT CGCTGTTGCT GCCCAGCCTC ACACTGCTGG 
     1261 TGTCCCACCT CTCCAGCTCC CAGGATGTCT CCAGTGAGCC CAGCAGTGAG CAGCAGCTGT 
     1321 GCGCCCTTAG CAAGCACCCC ACCGTGGCCT TTGAAGACCT GCAGCCGTGG GTCTCTAACT 
     1381 TCACCTACCC TGGAGCCCGG GATTTCTCCC AGCTGGCTTT GGACCCCTCC GGGAACCAGC 
     1441 TCATCGTGGG AGCCAGGAAC TACCTCTTCA GACTCAGCCT TGCCAATGTC TCTCTTCTTC 
     1501 AGGCCACAGA GTGGGCCTCC AGTGAGGACA CGCGCCGCTC CTGCCAAAGC AAAGGGAAGA 
     1561 CTGAGGAGGA GTGTCAGAAC TACGTGCGAG TCCTGATCGT CGCCGGCCGG AAGGTGTTCA 
     1621 TGTGTGGAAC CAATGCCTTT TCCCCCATGT GCACCAGCAG ACAGGTGGGG AACCTCAGCC 
     1681 GGACTATTGA GAAGATCAAT GGTGTGGCCC GCTGCCCCTA TGACCCACGC CACAACTCCA 
     1741 CAGCTGTCAT CTCCTCCCAG GGGGAGCTCT ATGCAGCCAC GGTCATCGAC TTCTCAGGTC 
     1801 GGGACCCTGC CATCTACCGC AGCCTGGGCA GTGGGCCACC GCTTCGCACT GCCCAATATA 
     1861 ACTCCAAGTG GCTTAATGAG CCAAACTTCG TGGCAGCCTA TGATATTGGG CTGTTTGCAT 
     1921 ACTTCTTCCT GCGGGAGAAC GCAGTGGAGC ACGACTGTGG ACGCACCGTG TACTCTCGCG 
     1981 TGGCCCGCGT GTGCAAGAAT GACGTGGGGG GCCGATTCCT GCTGGAGGAC ACATGGACCA 
     2041 CATTCATGAA GGCCCGGCTC AACTGCTCCC GCCCGGGCGA GGTCCCCTTC TACTATAACG 
     2101 AGCTGCAGAG TGCCTTCCAC TTGCCGGAGC AGGACCTCAT CTATGGAGTT TTCACAACCA 
     2161 ACGTAAACAG CATCGCGGCT TCTGCTGTCT GCGCCTTCAA CCTCAGTGCT ATCTCCCAGG 
     2221 CTTTCAATGG CCCATTTCGC TACCAGGAGA ACCCCAGGGC TGCCTGGCTC CCCATAGCCA 
     2281 ACCCCATCCC CAATTTCCAG TGTGGCACCC TGCCTGAGAC CGGTCCCAAC GAGAACCTGA 
     2341 CGGAGCGCAG CCTGCAGGAC GCGCAGCGCC TCTTCCTGAT GAGCGAGGCC GTGCAGCCGG 
     2401 TGACACCCGA GCCCTGTGTC ACCCAGGACA GCGTGCGCTT CTCACACCTC GTGGTGGACC 
     2461 TGGTGCAGGC TAAAGACACG CTCTACCATG TACTCTACAT TGGCACCGAG TCGGGCACCA 
     2521 TCCTGAAGGC GCTGTCCACG GCGAGCCGCA GCCTCCACGG CTGCTACCTG GAGGAGCTGC 
     2581 ACGTGCTGCC CCCCGGGCGC CGCGAGCCCC TGCGCAGCCT GCGCATCCTG CACAGCGCCC 
     2641 GCGCGCTCTT CGTGGGGCTG AGAGACGGCG TCCTGCGGGT CCCACTGGAG AGGTGCGCCG 
     2701 CCTACCGCAG CCAGGGGGCA TGCCTGGGGG CCCGGGACCC GTACTGTGGC TGGGACGGGA 
     2761 AGCAGCAACG TTGCAGCACA CTCGAGGACA GCTCCAACAT GAGCCTCTGG ACCCAGAACA 
     2821 TCACCGCCTG TCCTGTGCGG AATGTGACAC GGGATGGGGG CTTCGGCCCA TGGTCACCAT 
     2881 GGCAACCATG TGAGCACTTG GATGGGGACA ACTCAGGCTC TTGCCTGTGT CGAGCTCGAT 
     2941 CCTGTGATTC CCCTCGACCC CGCTGTGGGG GCCTTGACTG CCTGGGGCCA GCCATCCACA 
     3001 TCGCCAACTG CTCCAGGAAT GGGGCGTGGA CCCCGTGGTC ATCGTGGGCG CTGTGCAGCA 
     3061 CGTCCTGTGG CATCGGCTTC CAGGTCCGCC AGCGAAGTTG CAGCAACCCT GCTCCCCGCC 
     3121 ACGGGGGCCG CATCTGCGTG GGCAAGAGCC GGGAGGAACG GTTCTGTAAT GAGAACACGC 
     3181 CTTGCCCGGT GCCCATCTTC TGGGCTTCCT GGGGCTCCTG GAGCAAGTGC AGCAGCAACT 
     3241 GTGGAGGGGG CATGCAGTCG CGGCGTCGGG CCTGCGAGAA CGGCAACTCC TGCCTGGGCT 
     3301 GCGGCGTGGA GTTCAAGACG TGCAACCCCG AGGGCTGCCC CGAAGTGCGG CGCAACACCC 
     3361 CCTGGACGCC GTGGCTGCCC GTGAACGTGA CGCAGGGCGG GGCACGGCAG GAGCAGCGGT 
     3421 TCCGCTTCAC CTGCCGCGCG CCCCTTGCAG ACCCGCACGG CCTGCAGTTC GGCAGGAGAA 
     3481 GGACCGAGAC GAGGACCTGT CCCGCGGACG GCTCCGGCTC CTGCGACACC GACGCCCTGG 
     3541 TGGAGGTCCT CCTGCGCAGC GGGAGCACCT CCCCGCACAC GGTGAGCGGG GGCTGGGCCG 
     3601 CCTGGGGCCC GTGGTCGTCC TGCTCCCGGG ACTGCGAGCT GGGCTTCCGC GTCCGCAAGA 
     3661 GAACGTGCAC TAACCCGGAG CCCCGCAACG GGGGCCTGCC CTGCGTGGGC GATGCTGCCG 
     3721 AGTACCAGGA CTGCAACCCC CAGGCTTGCC CAGTTCGGGG TGCTTGGTCC TGCTGGACCT 
     3781 CATGGTCTCC ATGCTCAGCT TCCTGTGGTG GGGGTCACTA TCAACGCACC CGTTCCTGCA 
     3841 CCAGCCCCGC ACCCTCCCCA GGTGAGGACA TCTGTCTCGG GCTGCACACG GAGGAGGCAC 
     3901 TATGTGCCAC ACAGGCCTGC CCAGAAGGCT GGTCGCCCTG GTCTGAGTGG AGTAAGTGCA 
     3961 CTGACGACGG AGCCCAGAGC CGAAGCCGGC ACTGTGAGGA GCTCCTCCCA GGGTCCAGCG 
     4021 CCTGTGCTGG AAACAGCAGC CAGAGCCGCC CCTGCCCCTA CAGCGAGATT CCCGTCATCC 
     4081 TGCCAGCCTC CAGCATGGAG GAGGCCACCG ACTGTGCAGG GTTCAATCTC ATCCACTTGG 
     4141 TGGCCACGGG CATCTCCTGC TTCTTGGGCT CTGGGCTCCT GACCCTAGCA GTGTACCTGT 
     4201 CTTGCCAGCA CTGCCAGCGT CAGTCCCAGG AGTCCACACT GGTCCATCCT GCCACCCCCA 
     4261 ACCATTTGCA CTACAAGGGC GGAGGCACCC CGAAGAATGA AAAGTACACA CCCATGGAAT 
     4321 TCAAGACCCT GAACAAGAAT AACTTGATCC CTGATGACAG AGCCAACTTC TACCCATTGC 
     4381 AGCAGACCAA TGTGTACACG ACTACTTACT ACCCAAGCCC CCTGAACAAA CACAGCTTCC 
     4441 GGCCCGAGGC CTCACCTGGA CAACGGTGCT TCCCCAACAG CACGCGTACG CGGCCGCTCG 
     4501 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4561 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4621 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4681 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4741 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4801 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4861 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4921 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     4981 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5041 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5101 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5161 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5221 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5281 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5341 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5401 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5461 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5521 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5581 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5641 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     5701 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     5761 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     5821 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     5881 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     5941 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6001 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6061 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6121 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6181 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6241 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6301 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6361 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6421 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6481 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6541 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6601 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6661 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6721 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     6781 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     6841 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     6901 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     6961 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7021 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7081 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7141 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7201 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7261 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7321 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7381 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7441 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7501 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7561 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7621 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7681 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7741 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     7801 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     7861 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     7921 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     7981 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     8041 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8101 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8161 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8221 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8281 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//