LOCUS       RC235188                8325 bp    DNA     circular  SYN 
DEFINITION  RFC1 (Myc-DDK tagged) - Homo sapiens replication factor C (activator 1) 1, 145kDa (RFC1), transcript variant 2
ACCESSION   NM_001204747
VERSION     NM_001204747.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4482..4573 
                     /label="Myc-DDK"  
     misc_feature    4603..5188 
                     /label="PolyA signal"  
     misc_feature    4660..4679 
                     /label="Restriction Enzyme"  
     misc_feature    5464..6136 
                     /label="ColE1"  
     misc_feature    6566..7360 
                     /label="Kan/Neo"  
     misc_feature    7395..7697 
                     /label="SV40 ori"  
     misc_feature    7783..8260 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4464..4470 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4464 
                     /label="RFC1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGACATTCGG AAATTCTTTG GAGTAATACC AAGTGGAAAG AAACTTGTAA 
     1081 GTGAAACAGT AAAGAAGAAT GAGAAAACAA AGTCTGATGA AGAAACTTTA AAAGCAAAGA 
     1141 AAGGAATAAA GGAAATCAAG GTAAATAGCT CCCGTAAAGA GGATGACTTC AAACAAAAGC 
     1201 AACCAAGCAA GAAAAAGAGG ATCATCTATG ATTCAGATTC AGAGTCAGAG GAGACGTTGC 
     1261 AGGTAAAAAA TGCCAAAAAG CCACCAGAAA AACTGCCAGT ATCTTCTAAA CCTGGTAAAA 
     1321 TTTCACGGCA GGATCCTGTT ACATACATTT CAGAAACAGA TGAAGAAGAT GACTTTATGT 
     1381 GTAAGAAGGC GGCCTCTAAA TCAAAAGAGA ATGGAAGATC TACAAATAGT CATCTTGGAA 
     1441 CATCAAACAT GAAAAAGAAT GAAGAAAACA CTAAGACCAA GAATAAGCCT TTATCACCAA 
     1501 TAAAACTTAC ACCCACATCA GTACTTGATT ATTTTGGAAC TGGAAGTGTC CAAAGATCTA 
     1561 ATAAGAAGAT GGTGGCAAGC AAAAGAAAAG AGCTTTCACA AAATACAGAT GAGTCTGGAT 
     1621 TAAATGATGA AGCCATCGCC AAGCAATTAC AGCTTGATGA AGATGCGGAG CTGGAGAGGC 
     1681 AGTTGCATGA AGATGAAGAG TTTGCCAGAA CATTAGCCAT GTTGGATGAA GAACCCAAGA 
     1741 CCAAAAAGGC TCGAAAGGAC ACAGAAGCGG GAGAAACGTT TTCATCTGTC CAAGCCAATT 
     1801 TAAGTAAAGC AGAAAAACAT AAATATCCTC ATAAAGTAAA AACAGCACAA GTTTCAGATG 
     1861 AAAGAAAGAG CTACAGTCCT AGGAAGCAAA GTAAATATGA AAGTTCAAAA GAATCTCAGC 
     1921 AACATTCCAA GTCATCAGCT GACAAAATAG GAGAAGTCTC TTCTCCCAAG GCCAGTTCTA 
     1981 AGCTGGCAAT TATGAAAAGA AAAGAAGAGA GCTCTTATAA AGAAATAGAG CCTGTGGCCT 
     2041 CAAAAAGAAA AGAAAATGCC ATTAAATTGA AAGGAGAGAC AAAAACTCCT AAGAAAACCA 
     2101 AAAGTTCTCC AGCTAAAAAA GAGTCTGTAA GTCCTGAAGA TTCTGAAAAG AAACGCACTA 
     2161 ATTATCAAGC TTATCGAAGC TACTTAAATC GAGAAGGTCC CAAGGCTCTG GGCTCCAAAG 
     2221 AAATACCAAA GGGAGCTGAA AATTGCTTGG AAGGCCTTAT ATTTGTAATC ACAGGCGTGC 
     2281 TGGAGTCTAT TGAACGAGAT GAGGCCAAGT CTCTAATTGA ACGTTATGGG GGAAAAGTAA 
     2341 CAGGAAATGT CAGCAAGAAA ACAAATTATC TTGTCATGGG TCGTGATAGT GGACAGTCCA 
     2401 AGAGTGATAA GGCCGCAGCC TTGGGGACAA AAATTATTGA TGAAGATGGC CTGTTGAATC 
     2461 TGATTCGGAC TATGCCAGGC AAGAAATCCA AGTATGAAAT AGCAGTTGAA ACTGAGATGA 
     2521 AGAAAGAGTC CAAACTGGAG AGAACACCCC AAAAAAATGT CCAAGGAAAA AGAAAAATTA 
     2581 GTCCATCTAA AAAGGAATCA GAATCTAAAA AGAGCAGGCC GACTTCCAAA AGGGACAGTT 
     2641 TGGCAAAGAC AATAAAAAAG GAAACAGATG TGTTTTGGAA AAGCCTGGAT TTCAAGGAGC 
     2701 AGGTGGCTGA GGAGACAAGT GGTGACAGCA AGGCTAGGAA TTTGGCTGAT GACAGCAGTG 
     2761 AAAACAAAGT GGAAAATTTG CTCTGGGTGG ATAAATATAA GCCAACCTCG CTCAAGACCA 
     2821 TAATTGGACA GCAAGGTGAC CAGAGCTGTG CCAACAAACT CCTACGCTGG CTCCGAAACT 
     2881 GGCAAAAGAG TTCTTCCGAA GATAAAAAAC ACGCAGCAAA GTTTGGTAAA TTTTCCGGCA 
     2941 AAGATGATGG CTCTAGTTTT AAAGCAGCGT TGCTGTCAGG CCCTCCTGGT GTTGGCAAAA 
     3001 CCACCACAGC TTCCCTGGTG TGTCAGGAGT TGGGATACAG CTACGTGGAA CTGAATGCAA 
     3061 GTGACACCCG GAGTAAGAGC AGTTTGAAGG CGATTGTTGC TGAGTCACTG AACAATACCA 
     3121 GCATCAAAGG CTTTTATTCA AATGGAGCAG CCTCTTCAGT AAGCACGAAA CATGCTCTCA 
     3181 TCATGGATGA AGTAGATGGC ATGGCAGGCA ATGAGGATAG GGGAGGAATT CAGGAATTAA 
     3241 TTGGCCTGAT AAAACATACT AAAATTCCCA TTATTTGTAT GTGCAATGAT AGAAATCATC 
     3301 CCAAGATTCG CTCTCTGGTT CATTATTGTT TTGATCTTCG TTTTCAAAGA CCTCGGGTTG 
     3361 AACAGATTAA GGGTGCTATG ATGTCTATTG CATTTAAAGA AGGTTTAAAG ATTCCCCCTC 
     3421 CAGCTATGAA TGAAATAATT TTGGGAGCCA ATCAAGATAT CAGACAGGTT TTACATAATC 
     3481 TGAGTATGTG GTGTGCACGA AGTAAAGCAT TAACCTATGA CCAGGCCAAA GCTGATTCTC 
     3541 ACAGAGCCAA AAAGGATATC AAAATGGGCC CATTTGATGT TGCCCGGAAA GTGTTTGCAG 
     3601 CTGGAGAGGA GACTGCTCAC ATGTCACTTG TGGACAAGTC AGATCTCTTT TTTCATGATT 
     3661 ATTCAATAGC ACCCCTCTTC GTCCAGGAAA ATTACATACA CGTGAAGCCT GTAGCAGCAG 
     3721 GGGGTGACAT GAAAAAGCAC CTGATGCTTT TAAGCAGAGC AGCAGACAGC ATATGCGATG 
     3781 GTGACCTAGT GGACAGCCAG ATCCGGAGTA AGCAAAACTG GAGTCTTCTG CCTGCGCAGG 
     3841 CCATTTATGC CAGTGTTCTT CCTGGAGAGT TGATGAGGGG GTACATGACC CAGTTTCCCA 
     3901 CCTTCCCAAG CTGGCTGGGG AAGCACTCGT CTACAGGCAA ACATGATCGT ATTGTTCAGG 
     3961 ACCTGGCCTT GCATATGAGT CTCAGAACTT ACTCCAGCAA AAGGACTGTA AACATGGATT 
     4021 ATCTGTCGCT TCTAAGGGAT GCACTTGTAC AGCCCTTGAC CTCACAAGGA GTAGACGGAG 
     4081 TACAGGATGT TGTTGCACTT ATGGACACAT ATTATTTGAT GAAAGAAGAC TTTGAGAATA 
     4141 TCATGGAAAT CAGCAGCTGG GGTGGCAAAC CTAGTCCCTT TTCAAAGCTG GATCCCAAGG 
     4201 TGAAAGCAGC CTTCACAAGA GCTTACAATA AGGAAGCCCA CCTTACTCCA TACTCACTTC 
     4261 AAGCTATAAA GGCATCTAGA CACAGCACAA GCCCATCCCT GGATTCGGAA TACAATGAAG 
     4321 AATTAAATGA AGATGACTCT CAATCTGATG AGAAAGACCA AGATGCTATA GAAACTGATG 
     4381 CCATGATCAA GAAAAAGACA AAATCTTCAA AGCCTTCAAA ACCAGAAAAA GATAAGGAGC 
     4441 CCAGAAAAGG AAAAGGAAAA AGTTCGAAGA AAACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     4501 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     4561 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4621 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     4681 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     4741 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     4801 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     4861 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     4921 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     4981 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     5041 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     5101 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5161 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5221 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     5281 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     5341 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     5401 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     5461 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     5521 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     5581 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     5641 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     5701 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     5761 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     5821 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     5881 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     5941 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     6001 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     6061 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     6121 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     6181 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     6241 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     6301 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     6361 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     6421 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     6481 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     6541 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     6601 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     6661 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     6721 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     6781 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     6841 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     6901 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     6961 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     7021 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     7081 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     7141 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     7201 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     7261 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     7321 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     7381 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     7441 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     7501 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     7561 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7621 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7681 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     7741 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     7801 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     7861 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     7921 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     7981 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     8041 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     8101 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     8161 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     8221 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     8281 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//