LOCUS       RC235183                8298 bp    DNA     circular  SYN 
DEFINITION  MYBPC1 (Myc-DDK tagged) - Homo sapiens myosin binding protein C, slow type (MYBPC1), transcript variant 10
ACCESSION   NM_001254723
VERSION     NM_001254723.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4455..4546 
                     /label="Myc-DDK"  
     misc_feature    4576..5161 
                     /label="PolyA signal"  
     misc_feature    4633..4652 
                     /label="Restriction Enzyme"  
     misc_feature    5437..6109 
                     /label="ColE1"  
     misc_feature    6539..7333 
                     /label="Kan/Neo"  
     misc_feature    7368..7670 
                     /label="SV40 ori"  
     misc_feature    7756..8233 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4437..4443 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4437 
                     /label="MYBPC1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCAGAACCC ACTAAGAAAG AGGATGAAGA GGAAGTCTCC CCGCCTAGCG 
     1081 CCTTGCCTCC AGACTGGACC CTTGTCGAAA CTCCTCCTGG GGAGGAACAA GCCAAGCAGA 
     1141 ATGCCAACTC CCAGCTGTCC ATCTTGTTCA TTGAAAAACC TCAAGGAGGA ACAGTGAAAG 
     1201 TTGGTGAAGA TATCACCTTC ATAGCCAAAG TCAAGGCTGA AGATCTTCTG AGAAAACCCA 
     1261 CTATCAAATG GTTCAAAGGA AAATGGATGG ACCTGGCCAG CAAAGCCGGG AAGCACCTTC 
     1321 AGCTGAAGGA AACCTTTGAG AGGCACAGTC GGGTGTACAC ATTTGAGATG CAGATCATCA 
     1381 AGGCCAAAGA TAACTTTGCA GGAAATTACA GATGCGAGGT CACCTATAAG GATAAGTTTG 
     1441 ACAGCTGTTC ATTTGATCTT GAAGTGCACG AATCTACTGG GACTACTCCA AACATTGACA 
     1501 TCAGATCTGC TTTCAAGAGA AGTGGAGAAG GTCAAGAGGA TGCAGGAGAA CTTGACTTTA 
     1561 GTGGTCTCCT GAAACGTAGG GAGGTGAAGC AGCAGGAGGA AGAACCCCAG GTGGACGTAT 
     1621 GGGAGTTGCT GAAGAACGCG AAACCCAGTG AGTACGAGAA GATCGCCTTC CAGTATGGAA 
     1681 TCACCGACCT GCGCGGCATG CTCAAGCGAC TCAAGCGCAT GCGCAGAGAG GAGAAGAAGA 
     1741 GCGCAGCTTT TGCAAAAATT CTTGATCCTG CATATCAGGT TGACAAAGGA GGCAGAGTGA 
     1801 GGTTTGTTGT GGAGCTGGCA GATCCAAAGT TGGAGGTGAA ATGGTATAAA AATGGTCAAG 
     1861 AAATTCGACC CAGTACCAAA TACATCTTTG AACACAAAGG ATGCCAGAGA ATCCTGTTTA 
     1921 TCAATAACTG TCAGATGACA GATGATTCAG AGTATTATGT GACAGCCGGT GATGAGAAAT 
     1981 GTTCCACTGA GCTCTTCGTA AGAGAGCCTC CAATTATGGT GACCAAACAG CTGGAAGATA 
     2041 CAACTGCTTA TTGTGGGGAG AGAGTGGAAT TAGAATGTGA GGTGTCTGAA GATGATGCCA 
     2101 ATGTAAAATG GTTTAAGAAT GGTGAAGAGA TTATCCCTGG TCCAAAATCA AGATACCGAA 
     2161 TTAGAGTTGA GGGTAAAAAA CACATCTTGA TCATAGAGGG AGCAACAAAG GCTGATGCTG 
     2221 CAGAATATTC AGTAATGACA ACAGGAGGAC AATCATCTGC TAAACTTAGT GTTGACTTGA 
     2281 AACCTCTGAA GATTTTGACA CCTCTGACTG ATCAGACTGT AAATCTTGGA AAAGAAATCT 
     2341 GCCTGAAGTG TGAAATCTCT GAAAACATAC CAGGAAAATG GACTAAAAAT GGCCTACCTG 
     2401 TTCAGGAGAG TGACCGTCTA AAGGTGGTTC ACAAGGGAAG GATCCACAAG TTAGTGATAG 
     2461 CCAATGCCCT CACTGAAGAT GAAGGTGATT ATGTATTTGC ACCTGATGCC TACAATGTTA 
     2521 CTCTGCCTGC CAAAGTTCAT GTTATTGATC CTCCTAAGAT CATCCTGGAT GGTCTTGATG 
     2581 CTGACAACAC AGTGACAGTG ATTGCAGGAA ACAAGCTTCG TCTTGAGATC CCCATCAGCG 
     2641 GAGAACCACC TCCTAAAGCC ATGTGGAGCC GGGGAGATAA GGCTATTATG GAAGGCAGTG 
     2701 GCCGGATAAG AACAGAATCT TACCCTGATA GCAGCACTCT GGTCATTGAT ATAGCTGAAA 
     2761 GAGATGACTC TGGTGTTTAC CACATCAATC TGAAAAACGA AGCTGGAGAG GCACATGCAA 
     2821 GCATCAAGGT TAAAGTTGTG GACTTCCCTG ATCCTCCAGT GGCACCGACT GTGACAGAGG 
     2881 TGGGAGATGA CTGGTGTATC ATGAACTGGG AGCCTCCTGC CTACGACGGA GGCTCTCCAA 
     2941 TCCTAGGATA TTTTATTGAG AGGAAGAAGA AACAAAGCTC CAGGTGGATG AGGCTGAATT 
     3001 TTGATCTCTG CAAAGAAACA ACTTTTGAGC CCAAGAAGAT GATTGAAGGT GTGGCCTATG 
     3061 AGGTCCGCAT CTTTGCAGTC AATGCCATTG GCATCTCCAA GCCCAGTATG CCCTCCAGGC 
     3121 CTTTTGTTCC TTTGGCTGTA ACAAGCCCTC CTACTCTTCT GACTGTGGAC TCTGTCACTG 
     3181 ACACGACTGT CACGATGAGG TGGCGCCCCC CAGACCACAT TGGTGCAGCA GGTTTAGATG 
     3241 GCTATGTGCT AGAGTATTGC TTTGAAGGAA CTGAGGACTG GATAGTTGCA AACAAAGATC 
     3301 TGATTGACAA GACGAAGTTC ACCATCACAG GTCTGCCAAC AGATGCAAAG ATCTTTGTGC 
     3361 GTGTGAAGGC TGTTAATGCA GCTGGTGCCA GCGAGCCCAA GTACTATTCT CAGCCCATTC 
     3421 TCGTGAAGGA AATCATAGAA CCTCCAAAGA TTCGCATTCC AAGACACCTG AAGCAAACCT 
     3481 ATATCCGCAG AGTTGGAGAA GCTGTCAATC TGGTTATACC TTTCCAGGGA AAACCAAGAC 
     3541 CAGAATTAAC TTGGAAGAAG GATGGTGCAG AAATTGATAA GAATCAAATA AACATTCGCA 
     3601 ACTCTGAGAC TGATACAATC ATATTTATTA GAAAAGCAGA GAGGAGCCAC TCTGGGAAAT 
     3661 ATGATCTGCA AGTCAAAGTG GACAAATTCG TGGAGACCGC ATCAATTGAC ATCCAGATCA 
     3721 TTGACCGTCC AGGTCCACCC CAAATTGTGA AGATTGAGGA TGTCTGGGGA GAAAATGTCG 
     3781 CTCTCACATG GACTCCACCA AAGGATGATG GAAATGCTGC TATCACAGGC TATACCATTC 
     3841 AGAAGGCTGA CAAGAAGAGC ATGGAATGGT TTACTGTCAT TGAGCATTAT CATCGAACCA 
     3901 GTGCCACCAT TACTGAATTG GTCATAGGGA ATGAATATTA CTTCCGGGTC TTTTCTGAAA 
     3961 ACATGTGTGG CCTCAGTGAG GATGCCACCA TGACTAAAGA GAGTGCAGTG ATCGCCAGGG 
     4021 ATGGTAAAAT CTACAAAAAT CCAGTGTATG AAGACTTTGA TTTCTCAGAG GCACCCATGT 
     4081 TTACTCAGCC TTTGGTTAAC ACCTATGCCA TAGCTGGTTA CAATGCCACC CTAAACTGCA 
     4141 GTGTGAGAGG AAATCCTAAG CCTAAAATAA CCTGGATGAA AAACAAAGTT GCTATTGTGG 
     4201 ATGATCCAAG ATACAGGATG TTCAGCAACC AGGGAGTCTG TACCCTGGAA ATTCGCAAGC 
     4261 CCAGCCCCTA TGATGGAGGC ACTTACTGCT GCAAAGCAGT CAATGACCTT GGGACAGTGG 
     4321 AGATTGAATG CAAACTGGAG GTGAAAGGTG GGCTCTCCTT CTGCAGACTC CTCTTGCAAG 
     4381 GCGTACCTCC AAACATAATT GATTCGTATC TGCGAGACTT ACACTCAAGC AATCCTGAGG 
     4441 AATACACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     4501 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     4561 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4621 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     4681 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     4741 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     4801 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     4861 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     4921 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     4981 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     5041 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     5101 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     5161 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     5221 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     5281 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     5341 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     5401 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     5461 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     5521 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     5581 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     5641 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     5701 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     5761 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     5821 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     5881 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     5941 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     6001 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     6061 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     6121 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     6181 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     6241 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     6301 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     6361 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     6421 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     6481 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     6541 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     6601 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     6661 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     6721 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     6781 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     6841 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     6901 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     6961 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     7021 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     7081 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     7141 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     7201 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     7261 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     7321 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     7381 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     7441 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     7501 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     7561 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7621 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7681 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     7741 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     7801 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     7861 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     7921 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     7981 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     8041 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     8101 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     8161 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     8221 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     8281 AAATTTAACG CGAATTTT
//