LOCUS       RC235182                8283 bp    DNA     circular  SYN 
DEFINITION  MYBPC1 (Myc-DDK tagged) - Homo sapiens myosin binding protein C, slow type (MYBPC1), transcript variant 7
ACCESSION   NM_001254720
VERSION     NM_001254720.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4440..4531 
                     /label="Myc-DDK"  
     misc_feature    4561..5146 
                     /label="PolyA signal"  
     misc_feature    4618..4637 
                     /label="Restriction Enzyme"  
     misc_feature    5422..6094 
                     /label="ColE1"  
     misc_feature    6524..7318 
                     /label="Kan/Neo"  
     misc_feature    7353..7655 
                     /label="SV40 ori"  
     misc_feature    7741..8218 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4422..4428 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4422 
                     /label="MYBPC1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCAGAACCC ACTAAGAAAG AGGAACCAAG TAAAGAGAAG GAGGCCGGAA 
     1081 CTACACCAGC AAAAGACTGG ACCCTTGTCG AAACTCCTCC TGGGGAGGAA CAAGCCAAGC 
     1141 AGAATGCCAA CTCCCAGCTG TCCATCTTGT TCATTGAAAA ACCTCAAGGA GGAACAGTGA 
     1201 AAGTTGGTGA AGATATCACC TTCATAGCCA AAGTCAAGGC TGAAGATCTT CTGAGAAAAC 
     1261 CCACTATCAA ATGGTTCAAA GGAAAATGGA TGGACCTGGC CAGCAAAGCC GGGAAGCACC 
     1321 TTCAGCTGAA GGAAACCTTT GAGAGGCACA GTCGGGTGTA CACATTTGAG ATGCAGATCA 
     1381 TCAAGGCCAA AGATAACTTT GCAGGAAATT ACAGATGCGA GGTCACCTAT AAGGATAAGT 
     1441 TTGACAGCTG TTCATTTGAT CTTGAAGTGC ACGAATCTAC TGGGACTACT CCAAACATTG 
     1501 ACATCAGATC TGCTTTCAAG AGAAGTGGAG AAGGTCAAGA GGATGCAGGA GAACTTGACT 
     1561 TTAGTGGTCT CCTGAAACGT AGGGAGGTGA AGCAGCAGGA GGAAGAACCC CAGGTGGACG 
     1621 TATGGGAGTT GCTGAAGAAC GCGAAACCCA GTGAGTACGA GAAGATCGCC TTCCAGTATG 
     1681 GAATCACCGA CCTGCGCGGC ATGCTCAAGC GACTCAAGCG CATGCGCAGA GAGGAGAAGA 
     1741 AGAGCGCAGC TTTTGCAAAA ATTCTTGATC CTGCATATCA GGTTGACAAA GGAGGCAGAG 
     1801 TGAGGTTTGT TGTGGAGCTG GCAGATCCAA AGTTGGAGGT GAAATGGTAT AAAAATGGTC 
     1861 AAGAAATTCG ACCCAGTACC AAATACATCT TTGAACACAA AGGATGCCAG AGAATCCTGT 
     1921 TTATCAATAA CTGTCAGATG ACAGATGATT CAGAGTATTA TGTGACAGCC GGTGATGAGA 
     1981 AATGTTCCAC TGAGCTCTTC GTAAGAGAGC CTCCAATTAT GGTGACCAAA CAGCTGGAAG 
     2041 ATACAACTGC TTATTGTGGG GAGAGAGTGG AATTAGAATG TGAGGTGTCT GAAGATGATG 
     2101 CCAATGTAAA ATGGTTTAAG AATGGTGAAG AGATTATCCC TGGTCCAAAA TCAAGATACC 
     2161 GAATTAGAGT TGAGGGTAAA AAACACATCT TGATCATAGA GGGAGCAACA AAGGCTGATG 
     2221 CTGCAGAATA TTCAGTAATG ACAACAGGAG GACAATCATC TGCTAAACTT AGTGTTGACT 
     2281 TGAAACCTCT GAAGATTTTG ACACCTCTGA CTGATCAGAC TGTAAATCTT GGAAAAGAAA 
     2341 TCTGCCTGAA GTGTGAAATC TCTGAAAACA TACCAGGAAA ATGGACTAAA AATGGCCTAC 
     2401 CTGTTCAGGA GAGTGACCGT CTAAAGGTGG TTCACAAGGG AAGGATCCAC AAGTTAGTGA 
     2461 TAGCCAATGC CCTCACTGAA GATGAAGGTG ATTATGTATT TGCACCTGAT GCCTACAATG 
     2521 TTACTCTGCC TGCCAAAGTT CATGTTATTG ATCCTCCTAA GATCATCCTG GATGGTCTTG 
     2581 ATGCTGACAA CACAGTGACA GTGATTGCAG GAAACAAGCT TCGTCTTGAG ATCCCCATCA 
     2641 GCGGAGAACC ACCTCCTAAA GCCATGTGGA GCCGGGGAGA TAAGGCTATT ATGGAAGGCA 
     2701 GTGGCCGGAT AAGAACAGAA TCTTACCCTG ATAGCAGCAC TCTGGTCATT GATATAGCTG 
     2761 AAAGAGATGA CTCTGGTGTT TACCACATCA ATCTGAAAAA CGAAGCTGGA GAGGCACATG 
     2821 CAAGCATCAA GGTTAAAGTT GTGGACTTCC CTGATCCTCC AGTGGCACCG ACTGTGACAG 
     2881 AGGTGGGAGA TGACTGGTGT ATCATGAACT GGGAGCCTCC TGCCTACGAC GGAGGCTCTC 
     2941 CAATCCTAGG ATATTTTATT GAGAGGAAGA AGAAACAAAG CTCCAGGTGG ATGAGGCTGA 
     3001 ATTTTGATCT CTGCAAAGAA ACAACTTTTG AGCCCAAGAA GATGATTGAA GGTGTGGCCT 
     3061 ATGAGGTCCG CATCTTTGCA GTCAATGCCA TTGGCATCTC CAAGCCCAGT ATGCCCTCCA 
     3121 GGCCTTTTGT TCCTTTGGCT GTAACAAGCC CTCCTACTCT TCTGACTGTG GACTCTGTCA 
     3181 CTGACACGAC TGTCACGATG AGGTGGCGCC CCCCAGACCA CATTGGTGCA GCAGGTTTAG 
     3241 ATGGCTATGT GCTAGAGTAT TGCTTTGAAG GAACTGAGGA CTGGATAGTT GCAAACAAAG 
     3301 ATCTGATTGA CAAGACGAAG TTCACCATCA CAGGTCTGCC AACAGATGCA AAGATCTTTG 
     3361 TGCGTGTGAA GGCTGTTAAT GCAGCTGGTG CCAGCGAGCC CAAGTACTAT TCTCAGCCCA 
     3421 TTCTCGTGAA GGAAATCATA GAACCTCCAA AGATTCGCAT TCCAAGACAC CTGAAGCAAA 
     3481 CCTATATCCG CAGAGTTGGA GAAGCTGTCA ATCTGGTTAT ACCTTTCCAG GGAAAACCAA 
     3541 GACCAGAATT AACTTGGAAG AAGGATGGTG CAGAAATTGA TAAGAATCAA ATAAACATTC 
     3601 GCAACTCTGA GACTGATACA ATCATATTTA TTAGAAAAGC AGAGAGGAGC CACTCTGGGA 
     3661 AATATGATCT GCAAGTCAAA GTGGACAAAT TCGTGGAGAC CGCATCAATT GACATCCAGA 
     3721 TCATTGACCG TCCAGGTCCA CCCCAAATTG TGAAGATTGA GGATGTCTGG GGAGAAAATG 
     3781 TCGCTCTCAC ATGGACTCCA CCAAAGGATG ATGGAAATGC TGCTATCACA GGCTATACCA 
     3841 TTCAGAAGGC TGACAAGAAG AGCATGGAAT GGTTTACTGT CATTGAGCAT TATCATCGAA 
     3901 CCAGTGCCAC CATTACTGAA TTGGTCATAG GGAATGAATA TTACTTCCGG GTCTTTTCTG 
     3961 AAAACATGTG TGGCCTCAGT GAGGATGCCA CCATGACTAA AGAGAGTGCA GTGATCGCCA 
     4021 GGGATGGTAA AATCTACAAA AATCCAGTGT ATGAAGACTT TGATTTCTCA GAGGCACCCA 
     4081 TGTTTACTCA GCCTTTGGTT AACACCTATG CCATAGCTGG TTACAATGCC ACCCTAAACT 
     4141 GCAGTGTGAG AGGAAATCCT AAGCCTAAAA TAACCTGGAT GAAAAACAAA GTTGCTATTG 
     4201 TGGATGATCC AAGATACAGG ATGTTCAGCA ACCAGGGAGT CTGTACCCTG GAAATTCGCA 
     4261 AGCCCAGCCC CTATGATGGA GGCACTTACT GCTGCAAAGC AGTCAATGAC CTTGGGACAG 
     4321 TGGAGATTGA ATGCAAACTG GAGGTGAAAG TGATATATCA AGGAGTAAAT ACCCCTGGAC 
     4381 AACCAGTCTT CCTGGAGGGG CAGCAACAGT CATTGCACAA TAAGGATTTT ACGCGTACGC 
     4441 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     4501 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4561 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4621 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4681 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4741 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     4801 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     4861 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     4921 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     4981 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     5041 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     5101 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     5161 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     5221 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     5281 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     5341 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     5401 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     5461 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     5521 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     5581 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     5641 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     5701 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     5761 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     5821 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     5881 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     5941 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     6001 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     6061 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     6121 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     6181 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     6241 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     6301 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     6361 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     6421 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     6481 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     6541 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     6601 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     6661 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     6721 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     6781 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     6841 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     6901 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     6961 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     7021 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     7081 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     7141 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     7201 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     7261 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     7321 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     7381 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     7441 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     7501 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     7561 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     7621 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     7681 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     7741 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     7801 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     7861 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     7921 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     7981 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     8041 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     8101 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     8161 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     8221 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     8281 TTT
//