LOCUS       RC235181                8280 bp    DNA     circular  SYN 
DEFINITION  CPD (Myc-DDK tagged) - Homo sapiens carboxypeptidase D (CPD), transcript variant 2
ACCESSION   NM_001199775
VERSION     NM_001199775.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4437..4528 
                     /label="Myc-DDK"  
     misc_feature    4558..5143 
                     /label="PolyA signal"  
     misc_feature    4615..4634 
                     /label="Restriction Enzyme"  
     misc_feature    5419..6091 
                     /label="ColE1"  
     misc_feature    6521..7315 
                     /label="Kan/Neo"  
     misc_feature    7350..7652 
                     /label="SV40 ori"  
     misc_feature    7738..8215 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4419..4425 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4419 
                     /label="CPD"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGGTTTGTG CTTTCTGGAA ATCTGCATGG TGGCTCAGTG GTAGCAAGCT 
     1081 ATCCTTTTGA TGATTCTCCA GAACATAAGG CCACTGGAAT CTATAGCAAA ACCTCAGATG 
     1141 ATGAAGTATT TAAATACTTG GCAAAAGCTT ATGCTTCAAA CCACCCCATA ATGAAAACTG 
     1201 GTGAGCCTCA TTGTCCAGGA GATGAAGACG AGACTTTCAA AGATGGAATC ACAAACGGCG 
     1261 CACATTGGTA TGATGTGGAA GGTGGTATGC AAGATTACAA TTATGTGTGG GCCAACTGTT 
     1321 TTGAGATCAC ATTAGAACTG TCTTGTTGCA AGTACCCACC TGCTTCACAG CTTCGACAGG 
     1381 AATGGGAGAA CAATCGTGAG TCTTTGATCA CATTGATTGA AAAGGTTCAC ATTGGAGTGA 
     1441 AAGGATTTGT TAAAGATTCC ATAACAGGAT CTGGGTTAGA GAATGCAACC ATCTCAGTGG 
     1501 CTGGTATTAA TCATAATATC ACAACAGGCA GATTTGGTGA TTTCTACCGA TTACTTGTTC 
     1561 CTGGAACTTA CAACCTTACA GTAGTTTTAA CTGGGTATAT GCCATTGACT GTTACTAATG 
     1621 TAGTGGTGAA AGAAGGACCA GCCACAGAGG TGGATTTTTC TCTTAGGCCA ACTGTAACTT 
     1681 CAGTAATCCC TGACACGACA GAGGCTGTAT CAACTGCTAG CACAGTTGCT ATACCTAATA 
     1741 TTCTTTCTGG AACATCATCC TCCTACCAGC CAATTCAGCC AAAGGACTTT CACCACCACC 
     1801 ATTTCCCTGA TATGGAAATC TTCTTGAGAA GGTTTGCCAA TGAATATCCT AACATTACCC 
     1861 GGCTTTATTC CTTGGGAAAA TCAGTAGAGT CAAGAGAACT TTATGTGATG GAGATATCTG 
     1921 ATAATCCGGG TGTCCATGAA CCAGGTGAAC CAGAATTTAA GTACATTGGA AATATGCATG 
     1981 GAAATGAAGT GGTTGGAAGA GAACTGCTGT TGAACCTCAT AGAATACCTT TGTAAGAACT 
     2041 TTGGAACAGA CCCTGAAGTC ACAGATTTGG TTCATAACAC TAGAATTCAC CTTATGCCAT 
     2101 CCATGAATCC TGATGGGTAT GAAAAGTCCC AGGAAGGAGA TTCAATAAGT GTAATTGGCA 
     2161 GAAACAACAG CAACAACTTT GACCTGAACC GAAATTTCCC AGACCAGTTT GTTCAGATCA 
     2221 CAGATCCTAC GCAACCAGAA ACTATTGCTG TAATGAGCTG GATGAAGTCC TATCCATTTG 
     2281 TACTTTCAGC AAACCTGCAT GGAGGTTCTT TGGTGGTTAA CTACCCTTTT GATGATGATG 
     2341 AACAAGGACT TGCCACATAT AGTAAATCAC CAGATGATGC TGTGTTCCAA CAAATAGCAC 
     2401 TTTCTTATTC CAAGGAAAAT TCCCAGATGT TTCAAGGTAG ACCTTGCAAG AATATGTATC 
     2461 CTAATGAATA TTTTCCTCAT GGAATAACAA ATGGAGCTAG TTGGTATAAT GTGCCAGGAG 
     2521 GAATGCAGGA CTGGAACTAT TTACAAACAA ATTGCTTTGA AGTGACTATT GAACTAGGTT 
     2581 GTGTGAAATA TCCACTTGAG AAAGAGCTGC CAAACTTTTG GGAACAGAAT CGAAGATCAC 
     2641 TAATCCAGTT TATGAAACAG GTTCATCAGG GCGTCAGAGG ATTTGTTCTA GATGCCACAG 
     2701 ATGGCAGGGG TATATTAAAT GCCACCATTA GTGTTGCTGA GATTAATCAC CCAGTGACTA 
     2761 CTTACAAAAC TGGAGATTAC TGGCGTCTCT TGGTTCCAGG AACTTATAAA ATCACAGCAT 
     2821 CTGCTCGAGG GTATAATCCA GTTACCAAGA ATGTGACTGT CAAGAGTGAA GGCGCTATTC 
     2881 AGGTCAACTT CACACTTGTT CGATCCTCAA CAGATTCAAA CAATGAATCA AAGAAAGGAA 
     2941 AAGGGGCTAG CAGCAGCACC AATGATGCCA GTGATCCAAC TACTAAAGAG TTTGAAACTT 
     3001 TAATTAAAGA CCTTTCAGCG GAGAATGGTT TGGAAAGCCT CATGTTACGC TCCTCCTCAA 
     3061 ATCTGGCTCT GGCTCTTTAT CGATACCATT CCTACAAAGA CTTATCAGAG TTTCTGAGAG 
     3121 GACTTGTAAT GAACTATCCA CATATTACAA ATCTTACCAA TTTGGGACAG AGCACTGAAT 
     3181 ATCGTCACAT TTGGTCCCTT GAAATCTCCA ATAAGCCCAA TGTATCTGAG CCTGAAGAAC 
     3241 CAAAGATTCG TTTTGTTGCT GGTATCCATG GAAATGCGCC AGTTGGAACT GAACTGCTTT 
     3301 TGGCTCTGGC AGAATTTCTC TGCCTGAACT ACAAAAAGAA CCCAGCTGTT ACCCAATTGG 
     3361 TTGACAGGAC TAGGATTGTG ATTGTCCCTT CTCTAAATCC AGATGGGCGA GAGAGAGCTC 
     3421 AAGAGAAAGA CTGTACTTCA AAAATAGGAC AAACAAATGC TCGTGGCAAA GATTTGGATA 
     3481 CAGACTTCAC AAATAATGCC TCCCAACCTG AGACCAAAGC CATCATTGAA AATTTGATTC 
     3541 AAAAACAGGA CTTTAGTCTT TCTGTTGCCT TAGATGGTGG TTCCATGCTG GTCACATATC 
     3601 CTTATGACAA GCCAGTACAG ACAGTGGAAA ATAAAGAGAC TCTGAAGCAT TTGGCATCTC 
     3661 TTTATGCAAA TAATCATCCA TCCATGCACA TGGGTCAGCC CAGTTGCCCA AATAAATCAG 
     3721 ATGAGAATAT TCCAGGAGGA GTAATGCGTG GAGCAGAATG GCATAGTCAC CTGGGCAGCA 
     3781 TGAAGGATTA TAGTGTCACC TATGGCCATT GTCCGGAAAT CACAGTATAC ACAAGCTGCT 
     3841 GTTACTTTCC TAGTGCTGCA CGACTCCCTT CCTTGTGGGC AGACAATAAG AGATCTCTTC 
     3901 TTAGTATGTT AGTGGAGGTT CACAAGGGAG TTCATGGATT TGTTAAAGAT AAGACTGGAA 
     3961 AGCCAATCTC TAAAGCAGTC ATTGTACTTA ATGAAGGAAT AAAGGTACAA ACAAAAGAGG 
     4021 GAGGTTATTT CCATGTACTC TTAGCGCCAG GTGTCCATAA CATTATTGCC ATCGCTGATG 
     4081 GGTACCAGCA ACAACATTCA CAGGTCTTTG TGCATCATGA TGCAGCTAGT TCTGTGGTGA 
     4141 TAGTCTTTGA CACAGATAAC CGGATATTTG GTTTGCCAAG GGAGCTTGTG GTAACTGTAT 
     4201 CAGGTGCTAC TATGTCGGCA TTGATCCTAA CAGCTTGCAT TATTTGGTGC ATCTGCTCAA 
     4261 TCAAGTCTAA TAGACACAAG GATGGCTTTC ATCGGCTCAG GCAGCATCAT GATGAGTATG 
     4321 AAGATGAAAT TCGCATGATG TCTACCGGCT CCAAGAAGTC CCTCCTAAGC CATGAGTTCC 
     4381 AGGATGAAAC AGACACTGAA GAGGAAACAT TATATTCTAG CAAACATACG CGTACGCGGC 
     4441 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     4501 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4561 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4621 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4681 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4741 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4801 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     4861 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     4921 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     4981 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     5041 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     5101 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     5161 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     5221 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     5281 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     5341 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     5401 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     5461 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     5521 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     5581 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     5641 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     5701 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     5761 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     5821 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     5881 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     5941 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     6001 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     6061 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     6121 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     6181 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     6241 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     6301 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     6361 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     6421 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     6481 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     6541 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     6601 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     6661 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     6721 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     6781 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     6841 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     6901 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     6961 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     7021 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     7081 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     7141 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     7201 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     7261 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     7321 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     7381 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     7441 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     7501 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     7561 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     7621 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     7681 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     7741 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     7801 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     7861 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     7921 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     7981 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     8041 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     8101 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     8161 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     8221 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//