LOCUS       RC235180                8274 bp    DNA     circular  SYN 
DEFINITION  SLC4A7 (Myc-DDK tagged) - Homo sapiens solute carrier family 4, sodium bicarbonate cotransporter, member 7 (SLC4A7), transcript variant 2
ACCESSION   NM_001258379
VERSION     NM_001258379.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4431..4522 
                     /label="Myc-DDK"  
     misc_feature    4552..5137 
                     /label="PolyA signal"  
     misc_feature    4609..4628 
                     /label="Restriction Enzyme"  
     misc_feature    5413..6085 
                     /label="ColE1"  
     misc_feature    6515..7309 
                     /label="Kan/Neo"  
     misc_feature    7344..7646 
                     /label="SV40 ori"  
     misc_feature    7732..8209 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4413..4419 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4413 
                     /label="SLC4A7"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGGCTGAT GGGGCCGGCG AGCAGATGAG ACCGCTACTC ACCCGGGGTC 
     1081 CTGATGAAGA AGCTGTTGTG GATCTTGGCA AAACTAGCTC AACTGTGAAC ACCAAGTTTG 
     1141 AAAAAGAAGA ACTAGAAAGT CATAGAGCTG TATATATTGG TGTTCACGTC CCGTTTAGTA 
     1201 AAGAGAGTCG TCGGCGTCAT AGGCATCGCG GACACAAACA TCACCACCGG AGAAGAAAAG 
     1261 ATAAAGAATC AGATAAAGAA GATGGACGGG AATCTCCTTC TTATGATACA CCATCCCAGA 
     1321 GAGTTCAGTT TATCCTTGGT ACTGAAGATG ATGATGAAGA ACATATTCCC CATGATCTCT 
     1381 TCACGGAAAT GGATGAACTG TGTTACAGAG ATGGAGAAGA ATATGAATGG AAAGAAACTG 
     1441 CTAGATGGCT GAAATTTGAA GAGGATGTTG AAGATGGCGG TGACCGATGG AGTAAACCTT 
     1501 ATGTGGCAAC TCTCTCTTTG CACAGTCTTT TTGAACTAAG GAGTTGCATC CTCAATGGAA 
     1561 CAGTCATGCT GGATATGAGA GCAAGCACTC TAGATGAAAT AGCAGATATG GTATTAGACA 
     1621 ACATGATAGC TTCTGGCCAA TTAGACGAGT CCATACGAGA GAATGTCAGA GAAGCTCTTC 
     1681 TGAAGAGACA TCATCATCAG AATGAGAAAA GATTCACCAG TCGGATTCCT CTTGTTCGAT 
     1741 CTTTTGCAGA TATAGGCAAG AAACATTCTG ACCCTCACTT GCTTGAAAGG AATGGTATTT 
     1801 TGGCCTCTCC CCAGTCTGCT CCTGGAAACT TGGACAATAG TAAAAGTGGA GAAATTAAAG 
     1861 GTAATGGAAG TGGTGGAAGC AGAGAAAATA GTACTGTTGA CTTCAGCAAG GTTGATATGA 
     1921 ATTTCATGAG AAAAATTCCT ACGGGTGCTG AGGCATCCAA CGTCCTGGTG GGCGAAGTAG 
     1981 ACTTTTTGGA AAGGCCAATA ATTGCATTTG TGAGACTGGC TCCTGCTGTC CTCCTTACAG 
     2041 GGTTGACTGA GGTCCCTGTT CCAACCAGGT TTTTGTTTTT GTTATTGGGT CCAGCGGGCA 
     2101 AGGCACCACA GTACCATGAA ATTGGACGAT CAATAGCCAC TCTCATGACA GATGAGATTT 
     2161 TCCATGATGT AGCTTATAAA GCAAAAGACA GAAATGACCT CTTATCTGGA ATTGATGAAT 
     2221 TTTTAGATCA AGTAACTGTC CTACCTCCAG GAGAGTGGGA TCCTTCTATA CGCATAGAAC 
     2281 CACCAAAAAG TGTCCCTTCT CAGGAAAAGA GAAAGATTCC TGTGTTTCAC AATGGATCTA 
     2341 CCCCCACACT GGGTGAGACT CCTAAAGAGG CCGCTCATCA TGCTGGGCCT GAGCTACAGA 
     2401 GGACTGGACG GCTTTTTGGT GGTTTGATAC TTGACATCAA AAGGAAAGCA CCTTTTTTCT 
     2461 TGAGTGACTT CAAGGATGCA TTAAGCCTGC AGTGCCTGGC CTCGATTCTT TTCCTATACT 
     2521 GTGCCTGTAT GTCTCCTGTA ATCACTTTTG GAGGGCTGCT TGGAGAAGCT ACAGAAGGCA 
     2581 GAATAAGTGC AATAGAGTCT CTTTTTGGAG CATCATTAAC TGGGATTGCC TATTCATTGT 
     2641 TTGCTGGGCA ACCTCTAACA ATATTGGGGA GCACAGGTCC AGTTCTAGTG TTTGAAAAAA 
     2701 TTTTATATAA ATTCTGCAGA GATTATCAAC TTTCTTATCT GTCTTTAAGA ACCAGTATTG 
     2761 GTCTGTGGAC TTCTTTTTTG TGCATTGTTT TGGTTGCAAC AGATGCAAGC AGCCTTGTGT 
     2821 GTTATATTAC TCGATTTACA GAAGAGGCTT TTGCAGCCCT TATTTGCATC ATATTCATCT 
     2881 ACGAGGCTTT GGAGAAGCTC TTTGATTTAG GAGAAACATA TGCATTTAAT ATGCACAACA 
     2941 ACTTAGATAA ACTGACCAGC TACTCATGTG TATGTACTGA ACCTCCAAAC CCCAGCAATG 
     3001 AAACTCTAGC ACAATGGAAG AAAGATAATA TAACAGCACA CAATATTTCC TGGAGAAATC 
     3061 TTACTGTTTC TGAATGTAAA AAACTTCGTG GTGTATTCTT GGGGTCAGCT TGTGGTCATC 
     3121 ATGGACCTTA TATTCCAGAT GTGCTCTTTT GGTGTGTCAT CTTGTTTTTC ACAACATTTT 
     3181 TTCTGTCTTC ATTCCTCAAG CAATTTAAGA CCAAGCGTTA CTTTCCTACC AAGGTGCGAT 
     3241 CGACAATCAG TGATTTTGCT GTATTTCTCA CAATAGTAAT AATGGTTACA ATTGACTACC 
     3301 TTGTAGGAGT TCCATCTCCT AAACTTCATG TTCCTGAAAA ATTTGAGCCT ACTCATCCAG 
     3361 AGAGAGGGTG GATCATAAGC CCACTGGGAG ATAATCCTTG GTGGACCTTA TTAATAGCTG 
     3421 CTATTCCTGC TTTGCTTTGT ACCATTCTCA TCTTTATGGA TCAACAAATC ACAGCTGTAA 
     3481 TTATAAACAG AAAGGAACAC AAATTGAAGA AAGGAGCTGG CTATCACCTT GATTTGCTCA 
     3541 TGGTTGGCGT TATGTTGGGA GTTTGCTCTG TCATGGGACT TCCATGGTTT GTGGCTGCAA 
     3601 CAGTGTTGTC AATAAGTCAT GTCAACAGCT TAAAAGTTGA ATCTGAATGT TCTGCTCCAG 
     3661 GGGAACAACC CAAGTTTTTG GGAATTCGTG AACAGCGGGT TACAGGGCTA ATGATTTTTA 
     3721 TTCTAATGGG CCTCTCTGTG TTCATGACTT CAGTCCTAAA GTTTATTCCA ATGCCTGTTC 
     3781 TGTATGGTGT TTTCCTTTAT ATGGGAGTTT CCTCATTAAA AGGAATCCAG TTATTTGACC 
     3841 GTATAAAATT ATTTGGAATG CCTGCTAAGC ATCAGCCTGA TTTGATATAC CTCCGTTATG 
     3901 TGCCGCTCTG GAAGGTCCAT ATTTTCACAG TCATTCAGCT TACTTGTTTG GTCCTTTTAT 
     3961 GGGTGATAAA AGTTTCAGCT GCTGCAGTGG TTTTTCCCAT GATGGTTCTT GCATTAGTGT 
     4021 TTGTGCGCAA ACTCATGGAC CTGTGTTTCA CGAAGAGAGA ACTTAGTTGG CTTGATGATC 
     4081 TTATGCCAGA AAGTAAGAAA AAGAAAGAAG ATGACAAAAA GAAAAAAGAG AAAGAGGAAG 
     4141 CTGAACGGAT GCTTCAAGAT GATGATGATA CTGTGCACCT TCCATTTGAA GGGGGAAGTC 
     4201 TCTTGCAAAT TCCAGTCAAG GCCCTAAAAT ATAGTGTTGA TCCCTCAATT GTTAACATAT 
     4261 CAGATGAAAT GGCCAAAACT GCACAGTGGA AGGCACTTTC CATGAATACT GAGAATGCCA 
     4321 AAGTAACCAG ATCTAACATG AGTCCTGATA AACCTGTGAG TGTGAAAATA AGTTTTGAAG 
     4381 ATGAACCAAG AAAGAAATAC GTGGATGCTG AAACTTCATT AACGCGTACG CGGCCGCTCG 
     4441 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4501 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4561 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4621 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4681 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4741 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4801 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4861 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     4921 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     4981 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5041 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5101 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5161 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5221 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5281 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5341 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5401 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5461 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5521 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5581 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     5641 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     5701 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     5761 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     5821 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     5881 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     5941 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6001 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6061 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6121 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6181 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6241 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6301 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6361 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6421 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6481 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6541 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6601 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6661 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     6721 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     6781 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     6841 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     6901 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     6961 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7021 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7081 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7141 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7201 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7261 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7321 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7381 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7441 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7501 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7561 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7621 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7681 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     7741 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     7801 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     7861 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     7921 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     7981 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8041 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8101 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8161 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8221 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
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