LOCUS       RC235179                8271 bp    DNA     circular  SYN 
DEFINITION  SORCS1 (Myc-DDK tagged) - Homo sapiens sortilin-related VPS10 domain containing receptor 1 (SORCS1), transcript variant 4
ACCESSION   NM_001206570
VERSION     NM_001206570.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4428..4519 
                     /label="Myc-DDK"  
     misc_feature    4549..5134 
                     /label="PolyA signal"  
     misc_feature    4606..4625 
                     /label="Restriction Enzyme"  
     misc_feature    5410..6082 
                     /label="ColE1"  
     misc_feature    6512..7306 
                     /label="Kan/Neo"  
     misc_feature    7341..7643 
                     /label="SV40 ori"  
     misc_feature    7729..8206 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4410..4416 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4410 
                     /label="SORCS1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGAAAAGTT GGCGCCGGCG GCGGCTCCCA AGCCCGGCTG AGCGCGCTCC 
     1081 TCGCCGGCGC GGGGCTCTTG ATCCTCTGCG CCCCGGGCGT CTGCGGCGGC GGCTCCTGCT 
     1141 GCCCCTCGCC GCACCCCAGC TCCGCTCCAC GCTCGGCCTC GACCCCTAGG GGCTTTTCCC 
     1201 ACCAGGGGCG GCCAGGCAGG GCTCCTGCCA CGCCCCTGCC CCTCGTAGTG CGTCCCCTGT 
     1261 TCTCAGTGGC CCCCGGGGAC CGAGCGCTAT CCCTGGAGCG GGCTCGGGGC ACTGGGGCAT 
     1321 CCATGGCGGT TGCTGCACGC TCCGGCCGGA GGAGACGGAG CGGAGCGGAT CAGGAGAAGG 
     1381 CAGAACGGGG AGAGGGCGCG AGTCGGAGCC CCCGGGGAGT GCTAAGAGAT GGAGGGCAGC 
     1441 AGGAGCCTGG GACTCGGGAG CGGGACCCGG ACAAAGCCAC CCGCTTCCGG ATGGAGGAGC 
     1501 TGAGACTGAC CAGCACCACG TTTGCGCTGA CGGGAGACTC AGCACACAAC CAAGCCATGG 
     1561 TCCACTGGTC TGGCCACAAC AGCAGCGTGA TTCTCATTTT GACAAAGCTC TATGACTATA 
     1621 ACCTGGGGAG CATCACAGAG AGCTCGCTTT GGAGGTCAAC CGATTATGGA ACAACCTATG 
     1681 AGAAGCTGAA TGATAAAGTT GGTTTGAAAA CCATTTTGAG CTATCTCTAT GTGTGTCCTA 
     1741 CCAACAAGCG TAAGATAATG TTACTCACAG ACCCGGAGAT TGAGAGCAGT TTATTGATCA 
     1801 GCTCAGATGA AGGGGCAACT TATCAAAAGT ACCGGCTGAA CTTCTACATT CAAAGCTTGC 
     1861 TTTTTCACCC CAAACAAGAA GACTGGATTC TGGCATACAG TCAAGACCAA AAGTTATACA 
     1921 GCTCTGCTGA ATTTGGGAGA AGATGGCAGC TTATCCAAGA AGGGGTTGTA CCAAACAGGT 
     1981 TCTACTGGTC TGTGATGGGG TCAAATAAAG AACCAGACCT TGTGCATCTT GAGGCCAGAA 
     2041 CTGTGGATGG TCATTCACAT TATCTAACTT GCCGAATGCA GAACTGTACA GAGGCCAACA 
     2101 GGAATCAGCC TTTTCCAGGC TACATTGACC CAGACTCTTT GATTGTTCAG GATCATTATG 
     2161 TGTTTGTTCA GCTGACATCA GGAGGGCGGC CACATTACTA CGTGTCCTAC CGAAGGAATG 
     2221 CATTTGCCCA AATGAAGCTT CCGAAATATG CTTTGCCCAA GGACATGCAT GTTATCAGCA 
     2281 CCGATGAGAA TCAGGTGTTC GCAGCGGTCC AAGAATGGAA CCAGAATGAC ACGTACAACC 
     2341 TCTACATCTC AGACACACGT GGTGTCTACT TCACCCTGGC CTTGGAGAAT GTCCAGAGCA 
     2401 GCAGAGGCCC TGAGGGCAAC ATCATGATCG ACCTCTATGA GGTAGCAGGG ATAAAGGGAA 
     2461 TGTTCTTGGC TAACAAGAAG ATTGACAACC AAGTGAAGAC TTTCATCACA TATAACAAAG 
     2521 GCAGAGACTG GCGTTTGCTG CAGGCGCCGG ACACGGATCT AAGGGGGGAC CCCGTGCACT 
     2581 GCTTGCTGCC CTATTGCTCA CTACACCTTC ACCTGAAGGT CTCTGAGAAT CCCTACACAT 
     2641 CAGGGATCAT TGCCAGCAAA GACACAGCTC CAAGCATCAT AGTGGCATCA GGTAATATAG 
     2701 GTTCTGAATT GTCAGACACT GACATCAGCA TGTTTGTCTC TTCAGATGCA GGGAACACCT 
     2761 GGAGACAGAT CTTTGAAGAA GAGCACAGTG TTTTGTACCT GGATCAAGGT GGAGTCCTGG 
     2821 TTGCTATGAA ACACACATCT CTCCCAATTC GACATCTTTG GTTGAGTTTT GATGAAGGGA 
     2881 GATCTTGGAG CAAATACAGT TTCACATCTA TTCCACTTTT TGTGGATGGG GTTCTGGGTG 
     2941 AGCCTGGAGA AGAGACTCTC ATCATGACAG TGTTTGGACA CTTCAGCCAC CGCTCTGAAT 
     3001 GGCAGCTGGT CAAAGTAGAT TACAAGTCCA TTTTTGATAG ACGGTGTGCC GAAGAGGACT 
     3061 ACAGACCTTG GCAGCTGCAC AGCCAGGGGG AAGCATGTAT CATGGGAGCA AAAAGGATAT 
     3121 ATAAGAAGCG AAAATCAGAG CGGAAGTGTA TGCAAGGAAA ATATGCAGGA GCTATGGAAT 
     3181 CTGAACCCTG TGTCTGCACT GAGGCTGATT TTGATTGCGA CTATGGTTAT GAGCGACACA 
     3241 GCAATGGCCA GTGCCTGCCG GCATTTTGGT TCAATCCATC CTCTCTGTCA AAGGATTGCA 
     3301 GCTTGGGACA GAGTTACCTC AATAGTACTG GGTACAGGAA GGTGGTTTCC AATAATTGCA 
     3361 CTGATGGCGT AAGGGAACAG TACACTGCCA AACCGCAGAA GTGCCCAGGG AAAGCCCCGC 
     3421 GGGGGCTGCG GATAGTCACG GCTGATGGAA AGCTGACAGC GGAACAAGGA CACAACGTCA 
     3481 CTCTCATGGT GCAATTAGAA GAGGGTGATG TTCAGCGGAC ACTCATCCAA GTGGACTTTG 
     3541 GCGATGGTAT CGCGGTGTCT TACGTCAATC TCAGCTCCAT GGAAGATGGG ATCAAACACG 
     3601 TCTATCAGAA CGTGGGCATT TTCCGTGTGA CCGTGCAGGT GGACAACAGT CTGGGTTCTG 
     3661 ACAGCGCCGT CCTGTACTTA CATGTAACTT GTCCCTTGGA GCACGTGCAC CTGTCTCTTC 
     3721 CCTTTGTCAC CACAAAGAAC AAAGAGGTCA ATGCGACGGC AGTGCTGTGG CCCAGCCAAG 
     3781 TGGGCACCCT CACTTACGTG TGGTGGTACG GAAACAACAC GGAGCCTTTG ATCACCTTGG 
     3841 AGGGAAGCAT ATCCTTCAGA TTTACTTCAG AAGGAATGAA TACCATCACA GTGCAGGTCT 
     3901 CAGCTGGGAA TGCCATCCTA CAAGACACAA AGACCATCGC AGTATATGAG GAATTCCGGT 
     3961 CTCTTCGCTT GTCCTTTTCT CCAAACCTGG ATGACTACAA CCCGGACATC CCTGAGTGGA 
     4021 GGAGGGACAT CGGTCGAGTC ATCAAAAAAT CCCTGGTGGA AGCCACAGGG GTTCCAGGCC 
     4081 AGCACATCCT GGTGGCGGTG CTCCCTGGCT TACCCACCAC TGCTGAACTC TTTGTCCTAC 
     4141 CCTATCAGGA TCCAGCTGGA GAAAACAAAA GGTCAACTGA TGACCTGGAG CAGATATCAG 
     4201 AATTGCTGAT CCACACGCTC AACCAAAACT CAGTACACTT CGAGCTGAAG CCAGGAGTCC 
     4261 GAGTCCTTGT CCATGCTGCT CACTTAACAG CGGCCCCCCT GGTGGACCTC ACTCCAACCC 
     4321 ACAGTGGATC TGCCATGCTG ATGCTGCTCT CAGTGGTGTT TGTGGGGCTG GCAGTGTTCG 
     4381 TCATCTACAA GTTTAAAAGG AAGTATTTCC ATAGTTGCAC GCGTACGCGG CCGCTCGAGC 
     4441 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     4501 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4561 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4621 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4681 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4741 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     4801 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     4861 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     4921 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     4981 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     5041 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     5101 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5161 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     5221 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     5281 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     5341 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     5401 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     5461 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     5521 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     5581 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     5641 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     5701 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     5761 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     5821 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     5881 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     5941 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     6001 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     6061 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     6121 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     6181 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     6241 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     6301 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     6361 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     6421 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     6481 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     6541 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     6601 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     6661 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     6721 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     6781 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     6841 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     6901 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     6961 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     7021 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     7081 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     7141 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     7201 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     7261 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     7321 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     7381 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     7441 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     7501 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7561 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     7621 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     7681 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     7741 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     7801 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     7861 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     7921 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     7981 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     8041 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     8101 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     8161 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     8221 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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