LOCUS       RC235178                8274 bp    DNA     circular  SYN 
DEFINITION  KCNMA1 (Myc-DDK tagged) - Homo sapiens potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant 5
ACCESSION   NM_001271518
VERSION     NM_001271518.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4429..4520 
                     /label="Myc-DDK"  
     misc_feature    4550..5135 
                     /label="PolyA signal"  
     misc_feature    4607..4626 
                     /label="Restriction Enzyme"  
     misc_feature    5411..6083 
                     /label="ColE1"  
     misc_feature    6513..7307 
                     /label="Kan/Neo"  
     misc_feature    7342..7644 
                     /label="SV40 ori"  
     misc_feature    7730..8207 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4404..4411 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4404 
                     /label="KCNMA1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCAAATGGT GGCGGCGGCG GCGGCGGCAG CAGCGGCGGC GGCGGCGGCG 
     1081 GCGGAGGCAG CAGTCTTAGA ATGAGTAGCA ATATCCACGC GAACCATCTC AGCCTAGACG 
     1141 CGTCCTCCTC CTCCTCCTCC TCCTCTTCCT CTTCTTCTTC TTCCTCCTCC TCTTCCTCCT 
     1201 CGTCCTCGGT CCACGAGCCC AAGATGGATG CGCTCATCAT CCCGGTGACC ATGGAGGTGC 
     1261 CGTGCGACAG CCGGGGCCAA CGCATGTGGT GGGCTTTCCT GGCCTCCTCC ATGGTGACTT 
     1321 TCTTCGGGGG CCTCTTCATC ATCTTGCTCT GGCGGACGCT CAAGTACCTG TGGACCGTGT 
     1381 GCTGCCACTG CGGGGGCAAG ACGAAGGTTG TCTTAGTCTT TGCTCTCAGC ATCGGTGCAC 
     1441 TTGTAATATA CTTCATAGAT TCATCAAACC CAATAGAATC CTGCCAGAAT TTCTACAAAG 
     1501 ATTTCACATT ACAGATCGAC ATGGCTTTCA ACGTGTTCTT CCTTCTCTAC TTCGGCTTGC 
     1561 GGTTTATTGC AGCCAACGAT AAATTGTGGT TCTGGCTGGA AGTGAACTCT GTAGTGGATT 
     1621 TCTTCACGGT GCCCCCCGTG TTTGTGTCTG TGTACTTAAA CAGAAGTTGG CTTGGTTTGA 
     1681 GATTTTTAAG AGCTCTGAGA CTGATACAGT TTTCAGAAAT TTTGCAGTTT CTGAATATTC 
     1741 TTAAAACAAG TAATTCCATC AAGCTGGTGA ATCTGCTCTC CATATTTATC AGCACGTGGC 
     1801 TGACTGCAGC CGGGTTCATC CATTTGGTGG AGAATTCAGG GGACCCATGG GAAAATTTCC 
     1861 AAAACAACCA GGCTCTCACC TACTGGGAAT GTGTCTATTT ACTCATGGTC ACAATGTCCA 
     1921 CCGTTGGTTA TGGGGATGTT TATGCAAAAA CCACACTTGG GCGCCTCTTC ATGGTCTTCT 
     1981 TCATCCTCGG GGGACTGGCC ATGTTTGCCA GCTACGTCCC TGAAATCATA GAGTTAATAG 
     2041 GAAACCGCAA GAAATACGGG GGCTCCTATA GTGCGGTTAG TGGAAGAAAG CACATTGTGG 
     2101 TCTGCGGACA CATCACTCTG GAGAGTGTTT CCAACTTCCT GAAGGACTTT CTGCACAAGG 
     2161 ACCGGGATGA CGTCAATGTG GAGATCGTTT TTCTTCACAA CATCTCCCCC AACCTGGAGC 
     2221 TTGAAGCTCT GTTCAAACGA CATTTTACTC AGGTGGAATT TTATCAGGGT TCCGTCCTCA 
     2281 ATCCACATGA TCTTGCAAGA GTCAAGATAG AGTCAGCAGA TGCATGCCTG ATCCTTGCCA 
     2341 ACAAGTACTG CGCTGACCCG GATGCGGAGG ATGCCTCGAA TATCATGAGA GTAATCTCCA 
     2401 TAAAGAACTA CCATCCGAAG ATAAGAATCA TCACTCAAAT GCTGCAGTAT CACAACAAGG 
     2461 CCCATCTGCT AAACATCCCG AGCTGGAATT GGAAAGAAGG TGATGACGCA ATCTGCCTCG 
     2521 CAGAGTTGAA GTTGGGCTTC ATAGCCCAGA GCTGCCTGGC TCAAGGCCTC TCCACCATGC 
     2581 TTGCCAACCT CTTCTCCATG AGGTCATTCA TAAAGATTGA GGAAGACACA TGGCAGAAAT 
     2641 ACTACTTGGA AGGAGTCTCA AATGAAATGT ACACAGAATA TCTCTCCAGT GCCTTCGTGG 
     2701 GTCTGTCCTT CCCTACTGTT TGTGAGCTGT GTTTTGTGAA GCTCAAGCTC CTAATGATAG 
     2761 CCATTGAGTA CAAGTCTGCC AACCGAGAGA GCCGAAGCCG AAAGCGTATA TTAATTAATC 
     2821 CTGGAAACCA TCTTAAGATC CAAGAAGGTA CTTTAGGATT TTTCATCGCA AGTGATGCCA 
     2881 AAGAAGTTAA AAGGGCATTT TTTTACTGCA AGGCCTGTCA TGATGACATC ACAGATCCCA 
     2941 AAAGAATAAA AAAATGTGGC TGCAAACGGC TTGAAGATGA GCAGCCGTCA ACACTATCAC 
     3001 CAAAAAAAAA GCAACGGAAT GGAGGCATGC GGAACTCACC CAACACCTCG CCTAAGCTGA 
     3061 TGAGGCATGA CCCCTTGTTA ATTCCTGGCA ATGATCAGAT TGACAACATG GACTCCAATG 
     3121 TGAAGAAGTA CGACTCTACT GGGATGTTTC ACTGGTGTGC ACCCAAGGAG ATAGAGAAAG 
     3181 TCATCCTGAC TCGAAGTGAA GCTGCCATGA CCGTCCTGAG TGGCCATGTC GTGGTCTGCA 
     3241 TCTTTGGCGA CGTCAGCTCA GCCCTGATCG GCCTCCGGAA CCTGGTGATG CCGCTCCGTG 
     3301 CCAGCAACTT TCATTACCAT GAGCTCAAGC ACATTGTGTT TGTGGGCTCT ATTGAGTACC 
     3361 TCAAGCGGGA ATGGGAGACG CTTCATAACT TCCCCAAAGT GTCCATATTG CCTGGTACGC 
     3421 CATTAAGTCG GGCTGATTTA AGGGCTGTCA ACATCAACCT CTGTGACATG TGCGTTATCC 
     3481 TGTCAGCCAA TCAGAATAAT ATTGATGATA CTTCGCTGCA GGACAAGGAA TGCATCTTGG 
     3541 CGTCACTCAA CATCAAATCT ATGCAGTTTG ATGACAGCAT CGGAGTCTTG CAGGCTAATT 
     3601 CCCAAGGGTT CACACCTCCA GGAATGGATA GATCCTCTCC AGATAACAGC CCAGTGCACG 
     3661 GGATGTTACG TCAACCATCC ATCACAACTG GGGTCAACAT CCCCATCATC ACTGAACTAG 
     3721 TGAACGATAC TAATGTTCAG TTTTTGGACC AAGACGATGA TGATGACCCT GATACAGAAC 
     3781 TGTACCTCAC GCAGCCCTTT GCCTGTGGGA CAGCATTTGC CGTCAGTGTC CTGGACTCAC 
     3841 TCATGAGCGC GACGTACTTC AATGACAATA TCCTCACCCT GATACGGACC CTGGTGACCG 
     3901 GAGGAGCCAC GCCGGAGCTG GAGGCTCTGA TTGCTGAGGA AAACGCCCTT AGAGGTGGCT 
     3961 ACAGCACCCC GCAGACACTG GCCAATAGGG ACCGCTGCCG CGTGGCCCAG TTAGCTCTGC 
     4021 TCGATGGGCC ATTTGCGGAC TTAGGGGATG GTGGTTGTTA TGGTGATCTG TTCTGCAAAG 
     4081 CTCTGAAAAC ATATAATATG CTTTGTTTTG GAATTTACCG GCTGAGAGAT GCTCACCTCA 
     4141 GCACCCCCAG TCAGTGCACA AAGAGGTATG TCATCACCAA CCCGCCCTAT GAGTTTGAGC 
     4201 TCGTGCCGAC GGACCTGATC TTCTGCTTAA TGCAGTTTGA CCACAATGCC GGCCAGTCCC 
     4261 GGGCCAGCCT GTCCCATTCC TCCCACTCGT CGCAGTCCTC CAGCAAGAAG AGCTCCTCTG 
     4321 TTCACTCCAT CCCATCCACA GCAAACCGAC AGAACCGGCC CAAGTCCAGG GAGTCCCGGG 
     4381 ACAAACAGAA CAGAAAAGAA ATGGTTTACA GAAGCGGACC GACGCGTACG CGGCCGCTCG 
     4441 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4501 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4561 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4621 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4681 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4741 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4801 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4861 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     4921 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     4981 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5041 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5101 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5161 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5221 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5281 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5341 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5401 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5461 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5521 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5581 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     5641 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     5701 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     5761 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     5821 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     5881 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     5941 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6001 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6061 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6121 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6181 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6241 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6301 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6361 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6421 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6481 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6541 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6601 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6661 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     6721 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     6781 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     6841 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     6901 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     6961 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7021 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7081 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7141 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7201 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7261 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7321 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7381 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7441 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7501 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7561 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7621 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7681 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     7741 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     7801 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     7861 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     7921 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     7981 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8041 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8101 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8161 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8221 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
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