LOCUS       RC235177                8262 bp    DNA     circular  SYN 
DEFINITION  MYBPC1 (Myc-DDK tagged) - Homo sapiens myosin binding protein C, slow type (MYBPC1), transcript variant 8
ACCESSION   NM_001254721
VERSION     NM_001254721.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4419..4510 
                     /label="Myc-DDK"  
     misc_feature    4540..5125 
                     /label="PolyA signal"  
     misc_feature    4597..4616 
                     /label="Restriction Enzyme"  
     misc_feature    5401..6073 
                     /label="ColE1"  
     misc_feature    6503..7297 
                     /label="Kan/Neo"  
     misc_feature    7332..7634 
                     /label="SV40 ori"  
     misc_feature    7720..8197 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4401..4407 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4401 
                     /label="MYBPC1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCAGAACCC ACTAAGAAAG AGGAAAATGA AGTGCCAGCC CCAGCCCCAC 
     1081 CCCCGGAAGA ACCAAGTAAA GAGAAGGAGG CCGGAACTAC ACCAGCAAAA GACTGGACCC 
     1141 TTGTCGAAAC TCCTCCTGGG GAGGAACAAG CCAAGCAGAA TGCCAACTCC CAGCTGTCCA 
     1201 TCTTGTTCAT TGAAAAACCT CAAGGAGGAA CAGTGAAAGT TGGTGAAGAT ATCACCTTCA 
     1261 TAGCCAAAGT CAAGGCTGAA GATCTTCTGA GAAAACCCAC TATCAAATGG TTCAAAGGAA 
     1321 AATGGATGGA CCTGGCCAGC AAAGCCGGGA AGCACCTTCA GCTGAAGGAA ACCTTTGAGA 
     1381 GGCACAGTCG GGTGTACACA TTTGAGATGC AGATCATCAA GGCCAAAGAT AACTTTGCAG 
     1441 GAAATTACAG ATGCGAGGTC ACCTATAAGG ATAAGTTTGA CAGCTGTTCA TTTGATCTTG 
     1501 AAGTGCACGA ATCTACTGGG ACTACTCCAA ACATTGACAT CAGATCTGCT TTCAAGAGAA 
     1561 GGGAGGTGAA GCAGCAGGAG GAAGAACCCC AGGTGGACGT ATGGGAGTTG CTGAAGAACG 
     1621 CGAAACCCAG TGAGTACGAG AAGATCGCCT TCCAGTATGG AATCACCGAC CTGCGCGGCA 
     1681 TGCTCAAGCG ACTCAAGCGC ATGCGCAGAG AGGAGAAGAA GAGCGCAGCT TTTGCAAAAA 
     1741 TTCTTGATCC TGCATATCAG GTTGACAAAG GAGGCAGAGT GAGGTTTGTT GTGGAGCTGG 
     1801 CAGATCCAAA GTTGGAGGTG AAATGGTATA AAAATGGTCA AGAAATTCGA CCCAGTACCA 
     1861 AATACATCTT TGAACACAAA GGATGCCAGA GAATCCTGTT TATCAATAAC TGTCAGATGA 
     1921 CAGATGATTC AGAGTATTAT GTGACAGCCG GTGATGAGAA ATGTTCCACT GAGCTCTTCG 
     1981 TAAGAGAGCC TCCAATTATG GTGACCAAAC AGCTGGAAGA TACAACTGCT TATTGTGGGG 
     2041 AGAGAGTGGA ATTAGAATGT GAGGTGTCTG AAGATGATGC CAATGTAAAA TGGTTTAAGA 
     2101 ATGGTGAAGA GATTATCCCT GGTCCAAAAT CAAGATACCG AATTAGAGTT GAGGGTAAAA 
     2161 AACACATCTT GATCATAGAG GGAGCAACAA AGGCTGATGC TGCAGAATAT TCAGTAATGA 
     2221 CAACAGGAGG ACAATCATCT GCTAAACTTA GTGTTGACTT GAAACCTCTG AAGATTTTGA 
     2281 CACCTCTGAC TGATCAGACT GTAAATCTTG GAAAAGAAAT CTGCCTGAAG TGTGAAATCT 
     2341 CTGAAAACAT ACCAGGAAAA TGGACTAAAA ATGGCCTACC TGTTCAGGAG AGTGACCGTC 
     2401 TAAAGGTGGT TCACAAGGGA AGGATCCACA AGTTAGTGAT AGCCAATGCC CTCACTGAAG 
     2461 ATGAAGGTGA TTATGTATTT GCACCTGATG CCTACAATGT TACTCTGCCT GCCAAAGTTC 
     2521 ATGTTATTGA TCCTCCTAAG ATCATCCTGG ATGGTCTTGA TGCTGACAAC ACAGTGACAG 
     2581 TGATTGCAGG AAACAAGCTT CGTCTTGAGA TCCCCATCAG CGGAGAACCA CCTCCTAAAG 
     2641 CCATGTGGAG CCGGGGAGAT AAGGCTATTA TGGAAGGCAG TGGCCGGATA AGAACAGAAT 
     2701 CTTACCCTGA TAGCAGCACT CTGGTCATTG ATATAGCTGA AAGAGATGAC TCTGGTGTTT 
     2761 ACCACATCAA TCTGAAAAAC GAAGCTGGAG AGGCACATGC AAGCATCAAG GTTAAAGTTG 
     2821 TGGACTTCCC TGATCCTCCA GTGGCACCGA CTGTGACAGA GGTGGGAGAT GACTGGTGTA 
     2881 TCATGAACTG GGAGCCTCCT GCCTACGACG GAGGCTCTCC AATCCTAGGA TATTTTATTG 
     2941 AGAGGAAGAA GAAACAAAGC TCCAGGTGGA TGAGGCTGAA TTTTGATCTC TGCAAAGAAA 
     3001 CAACTTTTGA GCCCAAGAAG ATGATTGAAG GTGTGGCCTA TGAGGTCCGC ATCTTTGCAG 
     3061 TCAATGCCAT TGGCATCTCC AAGCCCAGTA TGCCCTCCAG GCCTTTTGTT CCTTTGGCTG 
     3121 TAACAAGCCC TCCTACTCTT CTGACTGTGG ACTCTGTCAC TGACACGACT GTCACGATGA 
     3181 GGTGGCGCCC CCCAGACCAC ATTGGTGCAG CAGGTTTAGA TGGCTATGTG CTAGAGTATT 
     3241 GCTTTGAAGG AACTGAGGAC TGGATAGTTG CAAACAAAGA TCTGATTGAC AAGACGAAGT 
     3301 TCACCATCAC AGGTCTGCCA ACAGATGCAA AGATCTTTGT GCGTGTGAAG GCTGTTAATG 
     3361 CAGCTGGTGC CAGCGAGCCC AAGTACTATT CTCAGCCCAT TCTCGTGAAG GAAATCATAG 
     3421 AACCTCCAAA GATTCGCATT CCAAGACACC TGAAGCAAAC CTATATCCGC AGAGTTGGAG 
     3481 AAGCTGTCAA TCTGGTTATA CCTTTCCAGG GAAAACCAAG ACCAGAATTA ACTTGGAAGA 
     3541 AGGATGGTGC AGAAATTGAT AAGAATCAAA TAAACATTCG CAACTCTGAG ACTGATACAA 
     3601 TCATATTTAT TAGAAAAGCA GAGAGGAGCC ACTCTGGGAA ATATGATCTG CAAGTCAAAG 
     3661 TGGACAAATT CGTGGAGACC GCATCAATTG ACATCCAGAT CATTGACCGT CCAGGTCCAC 
     3721 CCCAAATTGT GAAGATTGAG GATGTCTGGG GAGAAAATGT CGCTCTCACA TGGACTCCAC 
     3781 CAAAGGATGA TGGAAATGCT GCTATCACAG GCTATACCAT TCAGAAGGCT GACAAGAAGA 
     3841 GCATGGAATG GTTTACTGTC ATTGAGCATT ATCATCGAAC CAGTGCCACC ATTACTGAAT 
     3901 TGGTCATAGG GAATGAATAT TACTTCCGGG TCTTTTCTGA AAACATGTGT GGCCTCAGTG 
     3961 AGGATGCCAC CATGACTAAA GAGAGTGCAG TGATCGCCAG GGATGGTAAA ATCTACAAAA 
     4021 ATCCAGTGTA TGAAGACTTT GATTTCTCAG AGGCACCCAT GTTTACTCAG CCTTTGGTTA 
     4081 ACACCTATGC CATAGCTGGT TACAATGCCA CCCTAAACTG CAGTGTGAGA GGAAATCCTA 
     4141 AGCCTAAAAT AACCTGGATG AAAAACAAAG TTGCTATTGT GGATGATCCA AGATACAGGA 
     4201 TGTTCAGCAA CCAGGGAGTC TGTACCCTGG AAATTCGCAA GCCCAGCCCC TATGATGGAG 
     4261 GCACTTACTG CTGCAAAGCA GTCAATGACC TTGGGACAGT GGAGATTGAA TGCAAACTGG 
     4321 AGGTGAAAGT GATATATCAA GGAGTAAATA CCCCTGGACA ACCAGTCTTC CTGGAGGGGC 
     4381 AGCAACAGTC ATTGCACAAT AAGGATTTTA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     4441 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     4501 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     4561 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4621 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     4681 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     4741 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     4801 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     4861 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     4921 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     4981 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     5041 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     5101 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     5161 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     5221 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     5281 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     5341 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     5401 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     5461 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     5521 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     5581 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     5641 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     5701 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     5761 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     5821 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     5881 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     5941 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     6001 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     6061 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     6121 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     6181 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     6241 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     6301 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     6361 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     6421 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     6481 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     6541 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     6601 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     6661 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     6721 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     6781 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     6841 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     6901 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     6961 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     7021 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     7081 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     7141 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     7201 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     7261 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     7321 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     7381 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     7441 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     7501 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7561 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7621 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     7681 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     7741 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     7801 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     7861 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     7921 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     7981 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     8041 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     8101 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     8161 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     8221 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
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