LOCUS       RC235176                8250 bp    DNA     circular  SYN 
DEFINITION  DIAPH3 (Myc-DDK tagged) - Homo sapiens diaphanous-related formin 3 (DIAPH3), transcript variant 5
ACCESSION   NM_001258368
VERSION     NM_001258368.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4407..4498 
                     /label="Myc-DDK"  
     misc_feature    4528..5113 
                     /label="PolyA signal"  
     misc_feature    4585..4604 
                     /label="Restriction Enzyme"  
     misc_feature    5389..6061 
                     /label="ColE1"  
     misc_feature    6491..7285 
                     /label="Kan/Neo"  
     misc_feature    7320..7622 
                     /label="SV40 ori"  
     misc_feature    7708..8185 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4389..4395 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4389 
                     /label="DIAPH3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAACGGCAC CAGCCGCGGC TGCACCACCC GGCCCAAGGC TCAGCCGCTG 
     1081 GGACTCCCTA CCCTTCCTCA GCCTCTCTCC GCGGCTGCCG GGAAAGCAAG ATGCCGCGCA 
     1141 GGAAGGGCCC CCAACACCCT CCGCCGCCCA GTGGCCCCGA GGAGCCTGGG GAGAAGCGCC 
     1201 CCAAGTTTGA AGATATGAAT TTAAATGAAG ATAAAAAGGC ACCATTGCGG GAAAAGGACT 
     1261 TCAGTATCAA AAAAGAAATG GTGATGCAGT ACATTAATAC TGCTTCTAAG ACAGGAAGTC 
     1321 TTAAGAGAAG CCGACAGATC TCACCTCAGG AATTCATTCA TGAGCTGAAA ATGGGGTCTG 
     1381 CAGATGAGAG ACTTGTCACA TGCCTGGAGT CTCTCCGAGT GTCTTTGACC AGCAATCCTG 
     1441 TGAGTTGGGT GGAAAGCTTT GGACATGAAG GGCTTGGATT ATTATTAGAC ATTTTGGAAA 
     1501 AACTGATTAG TGGAAAAATC CAAGAAAAAG TTGTAAAGAA AAATCAACAT AAAGTCATAC 
     1561 AGTGTCTAAA AGCCCTGATG AATACGCAGT ATGGCTTGGA AAGAATTATG AGTGAGGAGA 
     1621 GGAGCCTTTC CTTATTGGCC AAAGCCGTGG ATCCCAGACA CCCCAATATG ATGACAGATG 
     1681 TGGTTAAACT TCTCTCTGCG GTATGCATTG TAGGGGAAGA AAGCATCCTT GAAGAAGTTT 
     1741 TAGAAGCTTT AACTTCAGCT GGTGAAGAAA AAAAAATTGA CAGATTTTTT TGTATTGTGG 
     1801 AAGGCCTCCG GCACAATTCA GTTCAACTGC AAGTAGCTTG TATGCAGCTC ATCAATGCCC 
     1861 TGGTTACATC TCCTGATGAT TTGGATTTCA GGCTTCACAT CAGAAATGAA TTTATGCGTT 
     1921 GTGGATTGAA AGAGATATTG CCAAATTTAA AATGCATTAA GAATGATGGC CTGGATATCC 
     1981 AACTTAAAGT CTTTGATGAG CATAAAGAAG AAGATTTGTT TGAGTTATCC CATCGCCTTG 
     2041 AAGATATTAG AGCTGAACTT GATGAAGCAT ATGATGTTTA CAACATGGTG TGGAGCACAG 
     2101 TTAAAGAAAC TAGAGCAGAG GGATATTTTA TTTCTATTCT TCAGCATCTT TTGCTGATTC 
     2161 GAAATGATTA TTTTATAAGG CAACAATACT TCAAATTAAT TGATGAGTGT GTATCCCAGA 
     2221 TTGTATTGCA TAGAGATGGA ATGGATCCAG ACTTCACATA TCGAAAAAGA CTAGATTTAG 
     2281 ATTTAACCCA GTTTGTAGAC ATTTGCATAG ATCAAGCAAA ACTAGAAGAG TTTGAAGAGA 
     2341 AAGCATCAGA ACTTTACAAG AAATTTGAAA AAGAGTTTAC CGACCACCAA GAAACTCAGG 
     2401 CTGAATTGCA GAAAAAAGAG GCAAAGATTA ATGAGCTTCA AGCAGAGCTA CAAGCTTTTA 
     2461 AGTCTCAGTT TGGTGCCTTG CCAGCTGATT GTAATATTCC TTTGCCTCCC TCTAAAGAAG 
     2521 GTGGAACTGG CCACTCAGCA CTTCCTCCTC CGCCTCCACT GCCTTCTGGT GGAGGGGTGC 
     2581 CGCCTCCACC TCCTCCCCCA CCACCTCCTC CACTTCCAGG AATGCGGATG CCATTCAGTG 
     2641 GTCCTGTGCC TCCACCACCT CCCCTGGGAT TCCTTGGAGG ACAAAATTCT CCTCCTCTAC 
     2701 CAATCCTGCC ATTTGGGTTG AAACCAAAGA AAGAATTTAA ACCTGAAATC AGCATGAGAA 
     2761 GATTGAATTG GTTAAAGATC AGACCTCATG AAATGACTGA AAACTGTTTC TGGATAAAAG 
     2821 TAAATGAAAA TAAGTATGAA AACGTGGATT TGCTTTGTAA ACTTGAGAAT ACATTTTGTT 
     2881 GCCAACAAAA AGAGAGAAGA GAAGAGGAAG ATATTGAAGA GAAGAAATCG ATTAAGAAAA 
     2941 AAATTAAAGA ACTTAAGTTT TTAGATTCTA AAATTGCCCA GAACCTTTCA ATCTTCCTGA 
     3001 GCTCTTTTCG GGTGCCATAT GAGGAAATCA GAATGATGAT ATTGGAAGTA GATGAAACAC 
     3061 GGTTGGCAGA GTCTATGATT CAGAACTTAA TAAAGCATCT TCCTGATCAA GAGCAATTAA 
     3121 ATTCATTGTC TCAGTTCAAG AGTGAATATA GCAACTTATG TGAACCTGAG CAGTTTGTGG 
     3181 TTGTGATGAG CAATGTGAAG AGACTACGGC CACGGCTCAG TGCTATTCTC TTTAAGCTTC 
     3241 AGTTTGAAGA GCAGGTGAAC AACATCAAAC CTGACATCAT GGCTGTCAGT ACTGCCTGCG 
     3301 AAGAGATAAA GAAGAGCAAA AGCTTTAGCA AGTTGCTGGA ACTTGTATTG CTAATGGGAA 
     3361 ACTACATGAA TGCTGGCTCC CGGAATGCTC AAACCTTCGG ATTTAACCTT AGCTCTCTCT 
     3421 GTAAACTAAA GGACACAAAA TCAGCAGATC AGAAAACAAC GCTACTTCAT TTCCTGGTAG 
     3481 AAATATGTGA AGAGAAGTAC CCTGATATAC TGAATTTTGT GGATGATTTG GAACCTTTAG 
     3541 ACAAAGCTAG TAAAGTCTCT GTAGAAACGC TGGAAAAGAA TTTGAGGCAG ATGGGAAGGC 
     3601 AGCTTCAACA GCTTGAGAAG GAATTGGAAA CCTTTCCCCC TCCTGAGGAC TTGCATGACA 
     3661 AGTTTGTGAC AAAGATGTCC AGATTTGTTA TCAGTGCAAA AGAACAATAT GAGACACTTT 
     3721 CGAAGTTACA CGAAAACATG GAAAAGTTAT ACCAGAGTAT AATAGGATAC TATGCCATTG 
     3781 ATGTGAAGAA GGTGTCTGTG GAAGACTTTC TTACTGACCT GAATAACTTC AGAACCACAT 
     3841 TCATGCAAGC AATAAAGGAG AATATCAAAA AAAGAGAAGC AGAGGAAAAA GAAAAACGTG 
     3901 TCAGAATAGC TAAAGAATTA GCAGAGCGAG AAAGACTCGA ACGCCAACAA AAGAAAAAGC 
     3961 GTTTATTAGA AATGAAGACT GAGGGTGATG AGACAGGAGT GATGGATAAT CTGCTGGAGG 
     4021 CCTTGCAGTC CGGGGCTGCC TTCCGCGACA GAAGAAAAAG GACACCGATG CCAAAAGATG 
     4081 TTCGGCAGAG TCTCAGTCCA ATGTCTCAGA GGCCTGTTCT GAAAGTTTGT AACCATGAAA 
     4141 ATCAGAAAGT GCAGTTGACA GAAGGGTCAC GTTCACACTA CAATATCAAT TGCAACTCAA 
     4201 CAAGGACTCC AGTCGCCAAG GAGCTTAATT ATAATCTAGA CACTCATACG TCTACTGGGA 
     4261 GGATCAAGGC AGCTGAGAAG AAGGAAGCGT GTAATGTAGA AAGCAACAGA AAAAAGGAAA 
     4321 CGGAACTTCT TGGCTCTTTT TCTAAAAATG AATCAGTTCC CGAAGTTGAA GCCCTGCTGG 
     4381 CAAGATTACG AGCTTTAACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     4441 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     4501 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     4561 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     4621 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     4681 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     4741 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     4801 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     4861 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     4921 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     4981 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     5041 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     5101 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     5161 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     5221 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     5281 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     5341 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     5401 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     5461 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     5521 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     5581 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     5641 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     5701 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     5761 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     5821 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     5881 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     5941 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     6001 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     6061 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     6121 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     6181 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     6241 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     6301 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     6361 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     6421 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     6481 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     6541 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     6601 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     6661 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     6721 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     6781 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     6841 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     6901 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     6961 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     7021 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     7081 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     7141 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     7201 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     7261 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     7321 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     7381 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     7441 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     7501 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7561 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7621 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     7681 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     7741 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     7801 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     7861 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     7921 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     7981 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     8041 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     8101 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     8161 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     8221 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//