LOCUS       RC235175                8247 bp    DNA     circular  SYN 
DEFINITION  TAOK2 (Myc-DDK tagged) - Homo sapiens TAO kinase 2 (TAOK2), transcript variant 3
ACCESSION   NM_001252043
VERSION     NM_001252043.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4404..4495 
                     /label="Myc-DDK"  
     misc_feature    4525..5110 
                     /label="PolyA signal"  
     misc_feature    4582..4601 
                     /label="Restriction Enzyme"  
     misc_feature    5386..6058 
                     /label="ColE1"  
     misc_feature    6488..7282 
                     /label="Kan/Neo"  
     misc_feature    7317..7619 
                     /label="SV40 ori"  
     misc_feature    7705..8182 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4386..4392 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4386 
                     /label="TAOK2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCAGCTGGG GGCCGGGCCG GGAGCCTGAA GGACCCAGAT GTGGCTGAGC 
     1081 TCTTCTTCAA GGATGACCCA GAAAAGCTCT TCTCTGACCT CCGGGAAATT GGCCATGGCA 
     1141 GCTTTGGAGC CGTATACTTT GCCCGGGATG TCCGGAATAG TGAGGTGGTG GCCATCAAGA 
     1201 AGATGTCCTA CAGTGGGAAG CAGTCCAATG AGAAATGGCA AGACATCATC AAGGAGGTGC 
     1261 GGTTCTTACA GAAGCTCCGG CATCCCAACA CCATTCAGTA CCGGGGCTGT TACCTGAGGG 
     1321 AGCACACGGC TTGGCTGGTA ATGGAGTATT GCCTGGGCTC AGCTTCTGAC CTTCTAGAAG 
     1381 TGCACAAGAA ACCCCTTCAG GAGGTAGAGA TCGCAGCTGT GACCCACGGG GCGCTTCAGG 
     1441 GCCTGGCATA TCTGCACTCC CACAACATGA TCCATAGGGA TGTGAAGGCT GGAAACATCC 
     1501 TGCTGTCAGA GCCAGGGTTA GTGAAGCTAG GGGACTTTGG TTCTGCGTCC ATCATGGCAC 
     1561 CTGCCAACTC CTTCGTGGGC ACCCCATACT GGATGGCACC CGAGGTGATC CTGGCCATGG 
     1621 ATGAGGGGCA GTACGATGGC AAAGTGGACG TCTGGTCCTT GGGGATAACC TGCATCGAGC 
     1681 TGGCTGAACG GAAACCACCG CTCTTTAACA TGAATGCGAT GAGTGCCTTA TACCACATTG 
     1741 CACAGAACGA ATCCCCCGTG CTCCAGTCAG GACACTGGTC TGAGTACTTC CGGAATTTTG 
     1801 TCGACTCCTG TCTTCAGAAA ATCCCTCAAG ACAGACCAAC CTCAGAGGTT CTCCTGAAGC 
     1861 ACCGCTTTGT GCTCCGGGAG CGGCCACCCA CAGTCATCAT GGACCTGATC CAGAGGACCA 
     1921 AGGATGCCGT GCGGGAGCTG GACAACCTGC AGTACCGCAA GATGAAGAAG ATCCTGTTCC 
     1981 AAGAGGCACC CAACGGCCCT GGTGCCGAGG CCCCAGAGGA GGAAGAGGAG GCCGAGCCCT 
     2041 ACATGCACCG GGCCGGGACT CTGACCAGCC TCGAGAGTAG CCACTCAGTG CCCAGCATGT 
     2101 CCATCAGCGC CTCCAGCCAG AGCAGCTCCG TCAACAGCCT AGCAGATGCC TCAGACAACG 
     2161 AGGAAGAGGA GGAGGAGGAG GAGGAAGAGG AGGAGGAGGA AGAAGGCCCT GAAGCCCGGG 
     2221 AGATGGCCAT GATGCAGGAG GGGGAGCACA CAGTCACCTC TCACAGCTCC ATTATCCACC 
     2281 GGCTGCCGGG CTCTGACAAC CTATATGATG ACCCCTACCA GCCAGAGATA ACCCCCAGCC 
     2341 CTCTCCAGCC GCCTGCAGCC CCAGCTCCCA CTTCCACCAC CTCTTCCGCC CGCCGCCGGG 
     2401 CCTACTGCCG TAACCGAGAC CACTTTGCCA CCATCCGAAC CGCCTCCCTG GTCAGCCGTC 
     2461 AGATCCAGGA GCATGAGCAG GACTCTGCGC TGCGGGAGCA GCTGAGCGGC TATAAGCGGA 
     2521 TGCGACGACA GCACCAGAAG CAGCTGCTGG CCCTGGAGTC ACGGCTGAGG GGTGAACGGG 
     2581 AGGAGCACAG TGCACGGCTG CAGCGGGAGC TTGAGGCGCA GCGGGCTGGC TTTGGGGCAG 
     2641 AGGCAGAAAA GCTGGCCCGG CGGCACCAGG CCATAGGTGA GAAGGAGGCA CGAGCTGCCC 
     2701 AGGCCGAGGA GCGGAAGTTC CAGCAGCACA TCCTTGGGCA GCAGAAGAAG GAGCTGGCTG 
     2761 CCCTGCTGGA GGCACAGAAG CGGACCTACA AACTTCGCAA GGAACAGCTG AAGGAGGAGC 
     2821 TCCAGGAGAA CCCCAGCACT CCCAAGCGGG AGAAGGCCGA GTGGCTGCTG CGGCAGAAGG 
     2881 AGCAGCTCCA GCAGTGCCAG GCGGAGGAGG AAGCAGGGCT GCTGCGGCGG CAGCGCCAGT 
     2941 ACTTTGAGCT GCAGTGTCGC CAGTACAAGC GCAAGATGTT GCTGGCTCGG CACAGCCTGG 
     3001 ACCAGGACCT GCTGCGGGAG GACCTGAACA AGAAGCAGAC CCAGAAGGAC TTGGAGTGTG 
     3061 CACTGCTGCT TCGGCAGCAC GAGGCCACGC GGGAGCTGGA GCTGCGGCAG CTCCAGGCCG 
     3121 TGCAGCGCAC GCGGGCTGAG CTCACCCGCC TGCAGCACCA GACGGAGCTG GGCAACCAGC 
     3181 TGGAGTACAA CAAGCGGCGT GAGCAAGAGT TGCGGCAGAA GCATGCGGCC CAGGTTCGCC 
     3241 AGCAGCCCAA GAGCCTCAAA GAGAGGAGCA TTGTTGGCCA GGAGGAGGCT GGGACATGGA 
     3301 GCTTGTGGGG GAAGGAGGAT GAGAGTCTTC TGGATGAGGA GTTTGAGCTT GGCTGGGTCC 
     3361 AGGGCCCAGC ACTGACTCCC GTCCCTGAGG AGGAGGAAGA AGAGGAAGAG GGGGCTCCGA 
     3421 TTGGGACCCC TAGGGATCCT GGAGATGGTT GTCCTTCCCC CGACATCCCT CCTGAACCCC 
     3481 CTCCAACACA CCTGAGGCCC TGCCCTGCCA GCCAGCTCCC TGGACTCCTG TCCCATGGCC 
     3541 TCCTGGCCGG CCTCTCCTTT GCAGTGGGGT CCTCCTCTGG CCTCCTGCCC CTCCTGCTGC 
     3601 TGCTGCTGCT TCCATTGCTG GCAGCCCAGG GTGGGGGTGG CCTGCAGGCA GCGCTGCTGG 
     3661 CCCTTGAGGT GGGGCTGGTG GGTCTGGGGG CCTCCTACCT GCTCCTTTGT ACAGCCCTGC 
     3721 ACCTGCCCTC CAGTCTTTTC CTACTCCTGG CCCAGGGTAC CGCACTGGGG GCCGTCCTGG 
     3781 GCCTGAGCTG GCGCCGAGGC CTCATGGGTG TTCCCCTGGG CCTTGGAGCT GCCTGGCTCT 
     3841 TAGCTTGGCC AGGCCTAGCT CTACCTCTGG TGGCTATGGC AGCGGGGGGC AGATGGGTGC 
     3901 GGCAGCAGGG CCCCCGGGTG CGCCGGGGCA TATCTCGACT CTGGTTGCGG GTTCTGCTGC 
     3961 GCCTGTCACC CATGGCCTTC CGGGCCCTGC AGGGCTGTGG GGCTGTGGGG GACCGGGGTC 
     4021 TGTTTGCACT GTACCCCAAA ACCAACAAGG ATGGCTTCCG CAGCCGCCTG CCCGTCCCTG 
     4081 GGCCCCGGCG GCGTAATCCC CGCACCACCC AACACCCATT AGCTCTGTTG GCAAGGGTCT 
     4141 GGGTCCTGTG CAAGGGCTGG AACTGGCGTC TGGCACGGGC CAGCCAGGGT TTAGCATCCC 
     4201 ACTTGCCCCC GTGGGCCATC CACACACTGG CCAGCTGGGG CCTGCTTCGG GGTGAACGGC 
     4261 CCACCCGAAT CCCCCGGCTA CTACCACGCA GCCAGCGCCA GCTAGGGCCC CCTGCCTCCC 
     4321 GCCAGCCACT GCCAGGGACT CTAGCCGGGC GGAGGTCACG CACCCGCCAG TCCCGGGCCC 
     4381 TGCCCCCCTG GAGGACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     4441 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     4501 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4561 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     4621 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     4681 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     4741 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     4801 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     4861 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     4921 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     4981 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     5041 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     5101 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     5161 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     5221 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     5281 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     5341 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     5401 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     5461 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     5521 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     5581 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     5641 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     5701 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     5761 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     5821 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     5881 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     5941 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     6001 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     6061 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     6121 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     6181 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     6241 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     6301 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     6361 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     6421 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     6481 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     6541 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     6601 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     6661 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     6721 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     6781 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     6841 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     6901 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     6961 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     7021 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     7081 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     7141 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     7201 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     7261 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     7321 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     7381 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     7441 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     7501 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7561 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7621 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     7681 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     7741 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     7801 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     7861 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     7921 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     7981 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     8041 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     8101 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     8161 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     8221 ATTTAACAAA AATTTAACGC GAATTTT
//