LOCUS       RC235174                8247 bp    DNA     circular  SYN 
DEFINITION  ASAP1 (Myc-DDK tagged) - Homo sapiens ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (ASAP1), transcript variant 2
ACCESSION   NM_001247996
VERSION     NM_001247996.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4404..4495 
                     /label="Myc-DDK"  
     misc_feature    4525..5110 
                     /label="PolyA signal"  
     misc_feature    4582..4601 
                     /label="Restriction Enzyme"  
     misc_feature    5386..6058 
                     /label="ColE1"  
     misc_feature    6488..7282 
                     /label="Kan/Neo"  
     misc_feature    7317..7619 
                     /label="SV40 ori"  
     misc_feature    7705..8182 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4386..4392 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4386 
                     /label="ASAP1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTTTCAAAT TTAAAGAGGA TTATGGCCCA GAAATGGATG CCGGACCAGA 
     1081 TCTCTGTCTC GGAGTTCATC GCCGAGACCA CCGAGGACTA CAACTCGCCC ACCACGTCCA 
     1141 GCTTCACCAC GCGGCTGCAC AACTGCAGGA ACACCGTCAC GCTGCTGGAG GAGGCTCTAG 
     1201 ACCAAGATAG AACAGCCCTT CAGAAAGTGA AGAAGTCTGT AAAAGCAATA TATAATTCTG 
     1261 GTCAAGATCA TGTACAAAAT GAAGAAAACT ATGCACAAGT TCTTGATAAG TTTGGGAGTA 
     1321 ATTTTTTAAG TCGAGACAAC CCCGACCTTG GCACCGCGTT TGTCAAGTTT TCTACTCTTA 
     1381 CAAAGGAACT GTCCACACTG CTGAAAAATC TGCTCCAGGG TTTGAGCCAC AATGTGATCT 
     1441 TCACCTTGGA TTCTTTGTTA AAAGGAGACC TAAAGGGAGT CAAAGGAGAT CTCAAGAAGC 
     1501 CATTTGACAA AGCCTGGAAA GATTATGAGA CAAAGTTTAC AAAAATTGAG AAAGAGAAAA 
     1561 GAGAGCACGC AAAACAACAT GGGATGATCC GCACAGAGAT AACAGGAGCT GAGATTGCGG 
     1621 AAGAAATGGA GAAGGAAAGG CGCCTCTTTC AGCTCCAAAT GTGTGAATAT CTCATTAAAG 
     1681 TTAATGAAAT CAAGACCAAA AAGGGTGTGG ATCTGCTGCA GAATCTTATA AAGTATTACC 
     1741 ATGCACAGTG CAATTTCTTT CAAGATGGCT TGAAAACAGC TGATAAGTTG AAACAGTACA 
     1801 TTGAAAAACT GGCTGCTGAT TTATATAATA TAAAACAGAC CCAGGATGAA GAAAAGAAAC 
     1861 AGCTAACTGC ACTCCGAGAC TTAATAAAAT CCTCTCTTCA ACTGGATCAG AAAGAAGATT 
     1921 CTCAGAGCCG GCAAGGAGGA TACAGCATGC ATCAGCTCCA GGGCAATAAG GAATATGGCA 
     1981 GTGAAAAGAA GGGGTACCTG CTAAAGAAAA GTGACGGGAT CCGGAAAGTA TGGCAGAGGA 
     2041 GGAAGTGTTC AGTCAAGAAT GGGATTCTGA CCATCTCACA TGCCACATCT AACAGGCAAC 
     2101 CAGCCAAGTT GAACCTTCTC ACCTGCCAAG TAAAACCTAA TGCCGAAGAC AAAAAATCTT 
     2161 TTGACCTGAT ATCACATAAT AGAACATATC ACTTTCAGGC AGAAGATGAG CAGGATTATG 
     2221 TAGCATGGAT ATCAGTATTG ACAAATAGCA AAGAAGAGGC CCTAACCATG GCCTTCCGTG 
     2281 GAGAGCAGAG TGCGGGAGAG AACAGCCTGG AAGACCTGAC AAAAGCCATT ATTGAGGATG 
     2341 TCCAGCGGCT CCCAGGGAAT GACATTTGCT GCGATTGTGG CTCATCAGAA CCCACCTGGC 
     2401 TTTCAACCAA CTTGGGTATT TTGACCTGTA TAGAATGTTC TGGCATCCAT AGGGAAATGG 
     2461 GGGTTCATAT TTCTCGCATT CAGTCTTTGG AACTAGACAA ATTAGGAACT TCTGAACTCT 
     2521 TGCTGGCCAA GAATGTAGGA AACAATAGTT TTAATGATAT TATGGAAGCA AATTTACCCA 
     2581 GCCCCTCACC AAAACCCACC CCTTCAAGTG ATATGACTGT ACGAAAAGAA TATATCACTG 
     2641 CAAAGTATGT AGATCATAGG TTTTCAAGGA AGACCTGTTC AACTTCATCA GCTAAACTAA 
     2701 ATGAATTGCT TGAGGCCATC AAATCCAGGG ATTTACTTGC ACTAATTCAA GTCTATGCAG 
     2761 AAGGGGTAGA GCTAATGGAA CCACTGCTGG AACCTGGGCA GGAGCTTGGG GAGACAGCCC 
     2821 TTCACCTTGC CGTCCGAACT GCAGATCAGA CATCTCTCCA TTTGGTTGAC TTCCTTGTAC 
     2881 AAAACTGTGG GAACCTGGAT AAGCAGACGG CCCTGGGAAA CACAGTTCTA CACTACTGTA 
     2941 GTATGTACAG TAAACCTGAG TGTTTGAAGC TTTTGCTCAG GAGCAAGCCC ACTGTGGATA 
     3001 TAGTTAACCA GGCTGGAGAA ACTGCCCTAG ACATAGCAAA GAGACTAAAA GCTACCCAGT 
     3061 GTGAAGATCT GCTTTCCCAG GCTAAATCTG GAAAGTTCAA TCCACACGTC CACGTAGAAT 
     3121 ATGAGTGGAA TCTTCGACAG GAGGAGATAG ATGAGAGCGA TGATGATCTG GATGACAAAC 
     3181 CAAGCCCTAT CAAGAAAGAG CGCTCACCCA GACCTCAGAG CTTCTGCCAC TCCTCCAGCA 
     3241 TCTCCCCCCA GGACAAGCTG GCACTGCCAG GATTCAGCAC TCCAAGGGAC AAACAGCGGC 
     3301 TCTCCTATGG AGCCTTCACC AACCAGATCT TCGTTTCCAC AAGCACAGAC TCGCCCACAT 
     3361 CACCAACCAC GGAGGCTCCC CCTCTGCCTC CTAGGAACGC CGGGAAAGGT CCAACTGGCC 
     3421 CACCTTCAAC ACTCCCTCTA AGCACCCAGA CCTCTAGTGG CAGCTCCACC CTATCCAAGA 
     3481 AGAGGCCTCC TCCCCCACCA CCCGGACACA AGAGAACCCT ATCCGACCCT CCCAGCCCAC 
     3541 TACCTCATGG GCCCCCAAAC AAAGGCGCAG TTCCTTGGGG TAACGATGGG GGTCCATCCT 
     3601 CTTCAAGTAA GACTACAAAC AAGTTTGAGG GACTATCCCA GCAGTCGAGC ACCAGTTCTG 
     3661 CAAAGACTGC CCTTGGCCCA AGAGTTCTTC CTAAACTACC TCAGAAAGTG GCACTAAGGA 
     3721 AAACAGATCA TCTCTCCCTA GACAAAGCCA CCATCCCGCC CGAAATCTTT CAGAAATCAT 
     3781 CACAGTTGGC AGAGTTGCCA CAAAAGCCAC CACCTGGAGA CCTGCCCCCA AAGCCCACAG 
     3841 AACTGGCCCC CAAGCCCCAA ATTGGAGATT TGCCGCCTAA GCCAGGAGAA CTGCCCCCCA 
     3901 AACCACAGCT GGGGGACCTG CCACCCAAAC CCCAACTCTC AGACTTACCT CCCAAACCAC 
     3961 AGATGAAGGA CCTGCCCCCC AAACCACAGC TGGGAGACCT GCTAGCAAAA TCCCAGACTG 
     4021 GAGATGTCTC ACCCAAGGCT CAGCAACCCT CTGAGGTCAC ACTGAAGTCA CACCCATTGG 
     4081 ATCTATCCCC AAATGTGCAG TCCAGAGACG CCATCCAAAA GCAAGCATCT GAAGACTCCA 
     4141 ACGACCTCAC GCCTACTCTG CCAGAGACGC CCGTACCACT GCCCAGAAAA ATCAATACGG 
     4201 GGAAAAATAA AGTGAGGCGA GTGAAGACCA TTTATGACTG CCAGGCAGAC AACGATGACG 
     4261 AGCTCACATT CATCGAGGGA GAAGTGATTA TCGTCACAGG GGAAGAGGAC CAGGAGTGGT 
     4321 GGATTGGCCA CATCGAAGGA CAGCCTGAAA GGAAGGGGGT CTTTCCAGTG TCCTTTGTTC 
     4381 ATATCCTGTC TGACACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     4441 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     4501 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4561 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     4621 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     4681 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     4741 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     4801 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     4861 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     4921 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     4981 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     5041 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     5101 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     5161 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     5221 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     5281 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     5341 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     5401 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     5461 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     5521 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     5581 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     5641 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     5701 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     5761 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     5821 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     5881 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     5941 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     6001 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     6061 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     6121 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     6181 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     6241 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     6301 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     6361 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     6421 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     6481 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     6541 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     6601 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     6661 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     6721 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     6781 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     6841 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     6901 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     6961 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     7021 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     7081 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     7141 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     7201 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     7261 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     7321 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     7381 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     7441 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     7501 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7561 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7621 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     7681 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     7741 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     7801 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     7861 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     7921 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     7981 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     8041 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     8101 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     8161 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     8221 ATTTAACAAA AATTTAACGC GAATTTT
//