LOCUS       RC235157                8121 bp    DNA     circular  SYN 
DEFINITION  ADCY4 (Myc-DDK tagged) - Homo sapiens adenylate cyclase 4 (ADCY4), transcript variant 1
ACCESSION   NM_001198592
VERSION     NM_001198592.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4276..4367 
                     /label="Myc-DDK"  
     misc_feature    4397..4982 
                     /label="PolyA signal"  
     misc_feature    4454..4473 
                     /label="Restriction Enzyme"  
     misc_feature    5258..5930 
                     /label="ColE1"  
     misc_feature    6360..7154 
                     /label="Kan/Neo"  
     misc_feature    7189..7491 
                     /label="SV40 ori"  
     misc_feature    7577..8054 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4251..4258 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4251 
                     /label="ADCY4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCCGCCTC TTCAGCCCCC GGCCGCCCCC CAGCGAAGAC CTCTTCTACG 
     1081 AGACCTACTA CAGCCTGAGC CAGCAGTACC CGCTGCTGCT GCTGCTGCTG GGGATCGTGC 
     1141 TCTGTGCGCT CGCGGCGCTG CTCGCAGTGG CCTGGGCCAG CGGCAGGGAG CTGACCTCAG 
     1201 ACCCGAGCTT CCTGACCACT GTGCTGTGCG CGCTGGGCGG CTTCTCGCTG CTGCTGGGCC 
     1261 TCGCTTCCCG GGAGCAGCGA CTGCAGCGCT GGACGCGTCC CCTGTCCGGC TTGGTATGGG 
     1321 TCGCGCTGCT AGCGCTAGGC CACGCCTTCC TGTTCACCGG GGGCGTGGTG AGCGCCTGGG 
     1381 ACCAGGTGTC CTATTTTCTC TTCGTCATCT TCACGGCGTA TGCCATGCTG CCCTTGGGCA 
     1441 TGCGGGACGC CGCCGTCGCG GGCCTCGCCT CCTCACTCTC GCATCTGCTG GTCCTCGGGC 
     1501 TGTATCTTGG GCCACAGCCG GACTCACGGC CTGCACTGCT GCCGCAGTTG GCAGCAAACG 
     1561 CAGTGCTGTT CCTGTGCGGG AACGTGGCAG GAGTGTACCA CAAGGCGCTG ATGGAGCGCG 
     1621 CCCTGCGGGC CACGTTCCGG GAGGCACTCA GCTCCCTGCA CTCACGCCGG CGGCTGGACA 
     1681 CCGAGAAGAA GCACCAGGAA CACCTTCTCT TGTCCATCCT TCCTGCCTAC CTGGCCCGAG 
     1741 AGATGAAGGC AGAGATCATG GCACGGCTGC AGGCAGGACA GGGGTCACGG CCAGAGAGCA 
     1801 CTAACAATTT CCACAGCCTC TATGTCAAGA GGCACCAGGG AGTCAGCGTG CTGTATGCTG 
     1861 ACATCGTGGG CTTCACGCGG CTGGCCAGCG AGTGTTCCCC TAAGGAGCTG GTGCTCATGC 
     1921 TCAATGAGCT CTTTGGCAAG TTCGACCAGA TTGCCAAGGA GCATGAATGC ATGCGGATCA 
     1981 AGATCCTGGG GGACTGTTAC TACTGTGTCT CTGGGCTGCC ACTCTCACTG CCAGACCATG 
     2041 CCATCAACTG CGTGCGCATG GGCCTGGACA TGTGCCGGGC CATCAGGAAA CTGCGGGCAG 
     2101 CCACTGGCGT GGACATCAAC ATGCGTGTGG GCGTGCACTC AGGCAGCGTA CTGTGTGGAG 
     2161 TCATCGGGCT GCAGAAGTGG CAGTACGACG TTTGGTCACA TGATGTCACA CTGGCTAACC 
     2221 ACATGGAGGC AGGCGGTGTA CCAGGGCGAG TGCACATCAC AGGGGCTACC CTGGCCCTGC 
     2281 TGGCAGGGGC TTATGCTGTG GAGGACGCAG GCATGGAGCA TCGGGACCCC TACCTTCGGG 
     2341 AGCTAGGGGA GCCTACCTAT CTGGTCATCG ATCCACGGGC AGAGGAGGAG GATGAGAAGG 
     2401 GCACTGCAGG AGGCTTGCTG TCCTCGCTTG AGGGCCTCAA GATGCGTCCA TCACTGCTGA 
     2461 TGACCCGTTA CCTGGAGTCC TGGGGCGCAG CCAAGCCTTT TGCCCACCTG AGCCACGGAG 
     2521 ACAGCCCTGT GTCCACCTCC ACCCCTCTCC CGGAGAAGAC CCTGGCTTCC TTCAGCACCC 
     2581 AGTGGAGCCT GGATCGGAGC CGTACCCCCC GGGGACTAGA TGATGAACTG GACACCGGGG 
     2641 ATGCCAAGTT CTTCCAGGTC ATTGAGCAGC TCAACTCGCA GAAACAGTGG AAGCAGTCGA 
     2701 AGGACTTCAA CCCACTGACA CTGTACTTCA GAGAGAAGGA GATGGAGAAA GAGTACCGAC 
     2761 TCTCTGCAAT CCCCGCCTTC AAATACTATG AAGCCTGCAC CTTCCTGGTT TTTCTCTCCA 
     2821 ACTTCATCAT CCAGATGCTA GTGACAAACA GGCCCCCAGC TCTGGCCATC ACGTATAGCA 
     2881 TCACCTTCCT CCTCTTCCTC CTCATCCTTT TTGTCTGCTT CTCAGAGGAC CTGATGAGGT 
     2941 GTGTCCTGAA AGGCCCCAAG ATGCTGCACT GGCTGCCTGC ACTGTCTGGC CTGGTGGCCA 
     3001 CACGACCAGG ACTGAGAATA GCCTTGGGCA CCGCCACCAT CCTCCTTGTC TTTGCCATGG 
     3061 CCATTACCAG CCTGTTCTTC TTCCCAACAT CATCAGACTG CCCTTTCCAA GCTCCCAATG 
     3121 TGTCCTCCAT GATTTCCAAC CTCTCCTGGG AGCTCCCTGG GTCTCTGCCT CTCATCAGTG 
     3181 TCCCATACTC CATGCACTGC TGCACGCTGG GCTTCCTCTC CTGCTCCCTC TTTCTGCACA 
     3241 TGAGCTTCGA GCTGAAGCTG CTGCTGCTCC TGCTGTGGCT GGCGGCATCC TGCTCCCTCT 
     3301 TCCTGCACTC CCATGCCTGG CTGTCGGAAT GCCTCATCGT CCGCCTCTAT CTGGGCCCCT 
     3361 TGGACTCCAG GCCCGGAGTG CTGAAGGAGC CCAAACTGAT GGGTGCTATC TCCTTCTTCA 
     3421 TCTTCTTCTT CACCCTCCTT GTCCTGGCTC GCCAGAATGA GTACTACTGC CGCCTGGACT 
     3481 TCCTGTGGAA GAAGAAGCTG AGGCAGGAGA GGGAGGAGAC AGAGACGATG GAGAACCTGA 
     3541 CTCGGCTGCT CTTGGAGAAC GTGCTCCCTG CACACGTGGC CCCCCAGTTC ATTGGCCAGA 
     3601 ACCGGCGCAA CGAGGATCTC TACCACCAGT CCTATGAATG CGTTTGTGTC CTCTTCGCCT 
     3661 CAGTCCCAGA CTTCAAGGAG TTCTACTCTG AATCCAACAT CAATCATGAG GGCCTAGAGT 
     3721 GTCTGAGGCT GCTCAATGAG ATAATTGCTG ATTTTGATGA GCTGCTCTCC AAGCCCAAGT 
     3781 TCAGTGGGGT GGAGAAGATC AAGACCATCG GCAGCACCTA CATGGCAGCC ACAGGCTTAA 
     3841 ATGCCACCTC TGGACAGGAT GCACAACAGG ATGCTGAACG GAGCTGCAGC CACCTTGGCA 
     3901 CTATGGTGGA ATTTGCCGTG GCCCTGGGGT CTAAGCTGGA CGTCATCAAC AAGCATTCAT 
     3961 TCAACAACTT CCGCCTGCGA GTGGGGTTGA ACCATGGACC CGTAGTAGCT GGAGTTATTG 
     4021 GGGCCCAGAA GCCGCAATAT GACATTTGGG GCAACACAGT GAACGTGGCC AGCCGCATGG 
     4081 AGAGTACAGG AGTCCTTGGC AAAATCCAAG TGACTGAGGA GACAGCATGG GCCCTACAGT 
     4141 CCCTGGGCTA CACCTGCTAC AGCCGGGGTG TCATCAAGGT GAAAGGCAAA GGGCAGCTCT 
     4201 GCACCTACTT CCTGAACACA GACTTGACAC GAACTGGACC TCCTTCAGCT ACCCTAGGCA 
     4261 GCGGACCGAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     4321 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     4381 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     4441 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     4501 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     4561 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     4621 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     4681 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     4741 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     4801 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     4861 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     4921 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     4981 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5041 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     5101 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     5161 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     5221 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     5281 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     5341 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     5401 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     5461 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     5521 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     5581 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     5641 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     5701 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     5761 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     5821 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     5881 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     5941 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     6001 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     6061 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     6121 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     6181 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     6241 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     6301 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     6361 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     6421 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     6481 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     6541 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     6601 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     6661 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     6721 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     6781 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     6841 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     6901 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     6961 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     7021 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     7081 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     7141 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     7201 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     7261 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     7321 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     7381 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7441 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7501 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     7561 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     7621 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     7681 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     7741 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     7801 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     7861 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     7921 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     7981 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     8041 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     8101 CAAAAATTTA ACGCGAATTT T
//