LOCUS       RC235150                8094 bp    DNA     circular  SYN 
DEFINITION  DAAM2 (Myc-DDK tagged) - Homo sapiens dishevelled associated activator of morphogenesis 2 (DAAM2), transcript variant 1
ACCESSION   NM_001201427
VERSION     NM_001201427.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4249..4340 
                     /label="Myc-DDK"  
     misc_feature    4370..4955 
                     /label="PolyA signal"  
     misc_feature    4427..4446 
                     /label="Restriction Enzyme"  
     misc_feature    5231..5903 
                     /label="ColE1"  
     misc_feature    6333..7127 
                     /label="Kan/Neo"  
     misc_feature    7162..7464 
                     /label="SV40 ori"  
     misc_feature    7550..8027 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4224..4231 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4224 
                     /label="DAAM2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCCCCCGC AAGAGGAGCC ACCATGGCCT GGGCTTCCTG TGCTGCTTCG 
     1081 GGGGCAGTGA CATCCCCGAA ATCAACCTCC GGGACAACCA CCCTCTGCAG TTCATGGAGT 
     1141 TCTCCAGCCC CATCCCGAAC GCAGAGGAGC TCAACATCCG CTTTGCAGAG CTGGTGGATG 
     1201 AATTGGATCT CACTGACAAA AACCGAGAGG CTATGTTTGC ACTGCCCCCT GAGAAGAAAT 
     1261 GGCAGATCTA CTGCAGCAAG AAGAAGGAGC AGGAGGACCC CAACAAGCTG GCAACCAGCT 
     1321 GGCCTGACTA TTACATCGAC CGCATCAATT CCATGGCTGC GATGCAGAGT CTGTACGCGT 
     1381 TTGATGAGGA GGAGACGGAG ATGAGGAACC AAGTCGTGGA AGACCTGAAG ACAGCCCTCC 
     1441 GGACACAGCC TATGAGGTTT GTGACCCGCT TCATTGAGCT GGAGGGCTTG ACCTGTCTGC 
     1501 TAAATTTCCT CCGGAGCATG GACCACGCCA CCTGTGAGAG CCGCATCCAC ACCTCACTCA 
     1561 TTGGCTGCAT CAAAGCATTG ATGAACAACT CCCAGGGGCG GGCACATGTG CTGGCACAGC 
     1621 CTGAGGCCAT TAGTACCATA GCCCAGAGCC TACGCACAGA GAACAGCAAG ACCAAGGTGG 
     1681 CTGTGCTGGA GATCCTGGGT GCTGTGTGCC TCGTGCCTGG TGGCCACAAG AAGGTGCTGC 
     1741 AGGCCATGCT GCACTACCAG GTGTATGCAG CAGAGCGAAC CCGCTTCCAG ACCCTGCTGA 
     1801 ACGAGCTAGA CCGAAGTCTG GGCCGGTACC GGGATGAAGT GAATCTGAAA ACAGCCATCA 
     1861 TGTCCTTCAT CAATGCTGTC CTCAATGCTG GAGCTGGAGA GGATAATCTG GAGTTCCGCC 
     1921 TACATCTACG GTATGAATTC CTGATGCTGG GTATACAGCC TGTGATTGAC AAGCTCCGGC 
     1981 AACATGAAAA TGCCATCCTG GACAAACATT TAGACTTCTT CGAGATGGTG CGGAATGAGG 
     2041 ATGACCTGGA GCTAGCCAGG AGGTTTGACA TGGTCCACAT CGACACCAAG AGTGCTTCCC 
     2101 AGATGTTTGA GTTGATCCAC AAGAAGCTGA AGTACACGGA GGCCTACCCC TGCCTGCTCT 
     2161 CTGTGCTGCA CCACTGCCTG CAGATGCCCT ACAAACGGAA CGGTGGCTAC TTCCAGCAGT 
     2221 GGCAGCTCCT GGACCGCATC CTCCAGCAGA TTGTCCTCCA GGATGAGCGG GGTGTGGACC 
     2281 CTGACCTGGC TCCCTTGGAG AACTTCAATG TCAAGAACAT CGTCAACATG CTCATCAACG 
     2341 AGAATGAAGT GAAACAGTGG CGAGACCAGG CAGAGAAGTT CCGGAAAGAA CACATGGAGC 
     2401 TTGTGAGCCG TCTGGAGAGG AAGGAGCGGG AATGCGAGAC AAAGACATTG GAGAAGGAAG 
     2461 AGATGATGCG GACGCTGAAC AAAATGAAGG ACAAGCTGGC CCGGGAGTCC CAGGAGCTGC 
     2521 GCCAGGCTCG GGGACAAGTG GCAGAGCTGG TAGCCCAGCT CAGTGAACTC TCAACAGGCC 
     2581 CTGTATCTTC CCCACCACCC CCTGGGGGCC CACTCACCTT GTCTTCCTCA ATGACAACCA 
     2641 ATGACCTGCC TCCACCCCCT CCTCCTCTGC CCTTTGCCTG TTGTCCCCCT CCCCCACCAC 
     2701 CACCCCTTCC TCCCGGGGGA CCCCCGACTC CCCCAGGTGC CCCACCTTGC CTCGGCATGG 
     2761 GCCTGCCCCT CCCTCAGGAC CCCTACCCCA GCAGTGACGT CCCACTCAGG AAAAAGCGTG 
     2821 TCCCCCAGCC TTCTCACCCA CTGAAGTCCT TCAACTGGGT GAAGCTGAAT GAGGAGCGTG 
     2881 TCCCTGGCAC CGTATGGAAT GAGATTGATG ACATGCAGGT ATTTCGGATC CTGGACCTAG 
     2941 AGGATTTTGA AAAGATGTTT TCAGCCTACC AGAGGCACCA GAAAGAGCTG GGCTCCACTG 
     3001 AAGACATCTA CCTGGCTTCC CGCAAGGTCA AAGAGCTGTC GGTCATTGAT GGCCGGAGGG 
     3061 CCCAAAACTG CATCATCCTT CTTTCCAAGT TGAAGCTTTC TAACGAGGAG ATCCGGCAGG 
     3121 CCATCTTGAA GATGGATGAG CAGGAGGACC TTGCTAAGGA CATGCTGGAG CAGCTCCTCA 
     3181 AGTTCATCCC AGAGAAGAGT GACATTGACC TCCTGGAGGA GCACAAGCAT GAAATTGAGC 
     3241 GGATGGCCCG TGCTGACCGC TTCCTCTATG AAATGAGCAG GATTGACCAC TACCAGCAGC 
     3301 GACTGCAAGC CCTCTTCTTC AAGAAGAAAT TCCAGGAGCG GCTGGCTGAG GCAAAGCCCA 
     3361 AAGTGGAAGC CATCCTGTTG GCCTCCCGGG AGCTGGTCCG CAGCAAGCGT CTTAGACAGA 
     3421 TGCTAGAGGT CATCCTAGCC ATAGGCAACT TCATGAACAA AGGGCAGCGT GGGGGCGCCT 
     3481 ACGGGTTCCG GGTGGCCAGC CTCAACAAGA TCGCTGACAC CAAGTCCAGC ATCGACAGAA 
     3541 ACATCTCTCT GCTCCATTAC CTGATCATGA TCCTGGAGAA GCATTTTCCT GATATTCTAA 
     3601 ACATGCCTTC AGAGCTGCAA CATCTTCCAG AAGCTGCCAA AGTCAACCTA GCAGAACTGG 
     3661 AGAAGGAGGT GGGCAACCTC AGGAGGGGCC TGAGAGCGGT GGAGGTGGAG CTGGAGTATC 
     3721 AGAGGCGCCA GGTACGGGAG CCCAGTGACA AGTTTGTCCC TGTCATGAGC GACTTCATCA 
     3781 CGGTGTCCAG CTTCAGCTTC TCCGAGCTGG AGGACCAGCT AAATGAGGCC AGGGACAAGT 
     3841 TCGCCAAGGC CTTGATGCAC TTCGGGGAGC ATGACAGCAA GATGCAGCCA GACGAATTCT 
     3901 TTGGCATCTT TGATACCTTC TTGCAGGCCT TCTCAGAGGC CCGGCAGGAT CTAGAGGCCA 
     3961 TGAGGAGGAG GAAGGAGGAG GAGGAGCGGC GGGCGCGCAT GGAAGCCATG CTGAAGGAGC 
     4021 AGAGGGAACG TGAGCGGTGG CAGCGGCAGC GGAAGGTCCT GGCTGCAGGC AGCTCGCTGG 
     4081 AGGAGGGAGG AGAGTTCGAT GACCTGGTGT CGGCCCTGCG CTCTGGGGAG GTCTTCGACA 
     4141 AGGACTTATG CAAGCTCAAG CGCAGCCGCA AGCGATCAGG GAGCCAGGCC CTGGAAGTTA 
     4201 CCCGGGAGCG GGCAATAAAC CGGCTAAATT ATAGCGGACC GACGCGTACG CGGCCGCTCG 
     4261 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4321 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4381 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4441 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4501 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4561 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4621 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4681 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     4741 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     4801 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     4861 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     4921 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     4981 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5041 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5101 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5161 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5221 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5281 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5341 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5401 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     5461 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     5521 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     5581 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     5641 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     5701 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     5761 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     5821 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     5881 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     5941 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6001 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6061 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6121 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6181 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6241 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6301 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6361 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6421 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6481 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     6541 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     6601 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     6661 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     6721 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     6781 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     6841 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     6901 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     6961 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7021 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7081 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7141 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7201 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7261 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7321 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7381 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7441 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7501 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     7561 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     7621 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     7681 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     7741 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     7801 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     7861 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     7921 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     7981 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8041 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//