LOCUS       RC235145                8064 bp    DNA     circular  SYN 
DEFINITION  ERCC6-PGBD3 (Myc-DDK tagged) - Homo sapiens ERCC6-PGBD3 readthrough (ERCC6-PGBD3), transcript variant 1
ACCESSION   NM_001277058
VERSION     NM_001277058.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4221..4312 
                     /label="Myc-DDK"  
     misc_feature    4342..4927 
                     /label="PolyA signal"  
     misc_feature    4399..4418 
                     /label="Restriction Enzyme"  
     misc_feature    5203..5875 
                     /label="ColE1"  
     misc_feature    6305..7099 
                     /label="Kan/Neo"  
     misc_feature    7134..7436 
                     /label="SV40 ori"  
     misc_feature    7522..7999 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4203..4209 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4203 
                     /label="ERCC6"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCAAATGAG GGAATCCCCC ACTCAAGTCA AACTCAGGAG CAAGACTGTT 
     1081 TACAGAGTCA ACCTGTCAGT AATAATGAAG AAATGGCAAT CAAGCAAGAA AGTGGTGGTG 
     1141 ATGGGGAGGT GGAGGAGTAC CTCTCCTTTC GTTCTGTGGG TGACGGGCTG TCCACCTCTG 
     1201 CTGTGGGGTG CGCATCAGCA GCTCCGAGGA GAGGGCCAGC CCTGCTGCAC ATCGACCGAC 
     1261 ATCAGATCCA GGCAGTAGAG CCTAGCGCCC AGGCCCTTGA GCTGCAGGGT TTGGGTGTGG 
     1321 ACGTCTATGA CCAGGACGTG CTGGAACAGG GAGTGCTTCA GCAGGTGGAC AATGCCATCC 
     1381 ATGAGGCCAG CCGTGCCTCC CAGCTCGTTG ACGTGGAGAA GGAGTATCGG TCGGTCCTGG 
     1441 ATGACCTCAC GTCATGTACG ACATCCCTAA GGCAAATCAA TAAAATTATT GAACAGCTTA 
     1501 GCCCTCAAGC TGCCACCAGC AGAGACATCA ACAGGAAACT AGATTCTGTA AAACGACAGA 
     1561 AGTATAATAA GGAACAACAG CTAAAAAAGA TCACTGCAAA ACAAAAGCAT CTCCAGGCCA 
     1621 TCCTTGGAGG AGCAGAGGTG AAAATTGAAC TAGATCACGC CAGTCTGGAG GAGGATGCAG 
     1681 AGCCGGGGCC ATCCAGTCTT GGCAGCATGC TCATGCCTGT CCAGGAGACT GCCTGGGAAG 
     1741 AGCTCATCCG CACTGGCCAG ATGACACCTT TTGGTACCCA GATCCCTCAG AAACAGGAGA 
     1801 AAAAGCCCAG AAAAATCATG CTTAATGAAG CATCAGGCTT CGAAAAGTAT TTGGCAGATC 
     1861 AAGCAAAACT GTCTTTTGAA AGGAAGAAGC AAGGTTGTAA TAAAAGAGCA GCTAGAAAAG 
     1921 CTCCAGCCCC AGTCACGCCT CCAGCCCCAG TGCAAAATAA AAACAAACCA AACAAGAAAG 
     1981 CCAGAGTTCT GTCCAAAAAA GAGGAGCGTT TGAAAAAGCA CATCAAGAAA CTCCAGAAGA 
     2041 GGGCTTTGCA GTTCCAGGGG AAAGTGGGAT TGCCAAAGGC AAGGAGACCT TGGGAGTCAG 
     2101 ACATGAGGCC AGAGGCAGAG GGAGACTCTG AGGGTGAAGA GTCTGAGTAT TTCCCCACAG 
     2161 AGGAGGAGGA AGAGGAGGAA GATGACGAGG TGGAGGGGGC AGAGGCGGAC CTGTCTGGAG 
     2221 ATGGTACTGA CTATGAGCTG AAGCCTCTGC CCAAGGGCGG GAAACGGCAG AAGAAAGTGC 
     2281 CAGTGCAGGA GATTGATGAT GACTTTTTCC CAAGTTCTGG GGAAGAAGCT GAAGCTGCTT 
     2341 CTGTAGGAGA AGGAGGAGGA GGAGGTCGGA AAGTGGGAAG ATACCGAGAT GATGGAGATG 
     2401 AAGATTATTA TAAGCAGCGG TTAAGTCCCA AGATGCCTCG AACACTAAGT TTACATGAAA 
     2461 TAACTGACCT TTTAGAGACA GATGACAGCA TAGAAGCAAG TGCTATAGTG ATACAACCAC 
     2521 CTGAAAATGC TACAGCACCT GTTTCTGATG AGGAATCAGG AGATGAAGAA GGTGGAACAA 
     2581 TAAATAATCT GCCAGGTTCT TTGTTGCACA CAGCTGCGTA TCTTATTCAA GATGGCTCTG 
     2641 ATGCTGAGTC TGACTCAGAT GATCCCTCAT ACGCACCTAA AGATGACTCT CCTGATGAAG 
     2701 TTCCATCTAC GTTTACTGTG CAGCAACCTC CACCATCAAG GAGGAGGAAA ATGACAAAAA 
     2761 TTCTTTGCAA ATGGAAAAAA GCCGACCTAA CTGTACAACC CGTAGCAGGT AGAGTTACAG 
     2821 CACCACCAAA CGATTTCTTC ACCGTAATGA GAACTCCCAC AGAAATTCTT GAACTTTTTC 
     2881 TTGATGACGA GGTCATTGAA CTCATTGTCA AGTACTCCAA CTTATATGCT TGCAGTAAAG 
     2941 GTGTACATCT TGGCTTGACT AGCTCTGAAT TCAAATGTTT TCTGGGAATT ATTTTTCTGA 
     3001 GTGGTTATGT CTCAGTTCCT AGAAGGCGTA TGTTTTGGGA ACAAAGAACA GATGTGCATA 
     3061 ATGTACTGGT TAGTGCTGCC ATGAGACGTG ACCGGTTTGA AACTATATTT TCTAATTTGC 
     3121 ATGTTGCTGA CAATGCAAAT TTGGATCCAG TGGACAAATT TTCCAAATTG CGACCTCTCA 
     3181 TAAGCAAACT TAATGAGAGA TGCATGAAAT TTGTTCCAAA TGAAACATAT TTCAGCTTTG 
     3241 ATGAATTCAT GGTTCCTTAT TTTGGTCGTC ACGGGTGCAA ACAATTTATT CGGGGAAAGC 
     3301 CCATTCGGTT TGGCTATAAG TTTTGGTGTG GTGCCACCTG TCTGGGCTAC ATTTGCTGGT 
     3361 TTCAGCCGTA TCAGGGTAAA AACCCAAATA CTAAACATGA GGAATATGGT GTCGGTGCGT 
     3421 CACTTGTCCT TCAGTTTAGT GAGGCACTTA CAGAGGCACA CCCTGGACAA TACCATTTTG 
     3481 TATTCAATAA CTTTTTCACC AGTATTGCAC TTCTTGATAA GCTCAGTTCA ATGGGACATC 
     3541 AGGCAACAGG TACAGTGAGA AAGGATCACA TTGACAGAGT TCCACTGGAA TCAGATGTAG 
     3601 CTTTAAAGAA AAAAGAAAGA GGCACATTTG ATTATCGAAT TGATGGCAAA GGCAATATTG 
     3661 TCTGCAGATG GAATGATAAC AGTGTTGTCA CTGTTGCCTC ATCTGGTGCT GGTATCCATC 
     3721 CCCTGTGTCT TGTCAGTCGT TACTCCCAGA AACTGAAAAA GAAGATACAA GTTCAGCAGC 
     3781 CAAACATGAT CAAAGTGTAT AACCAGTTCA TGGGAGGCGT AGACAGAGCT GATGAAAACA 
     3841 TTGATAAGTA TCGGGCATCA ATCCGTGGAA AGAAATGGTA TTCAAGCCCT CTTTTGTTCT 
     3901 GTTTCGAACT GGTCTTACAA AATGCTTGGC AATTGCATAA AACATATGAT GAGAAACCAG 
     3961 TGGATTTTCT GGAGTTTCGT CGACGTGTGG TATGCCATTA TCTGGAGACC CATGGTCATC 
     4021 CTCCAGAACC TGGCCAAAAA GGAAGACCTC AGAAGCGTAA CATTGACTCA CGTTATGATG 
     4081 GCATAAATCA TGTGATAGTC AAACAGGGAA AGCAAACGCG ATGCGCTGAA TGTCATAAGA 
     4141 ACACAACTTT TCGATGTGAA AAATGTGATG TTGCCTTACA TGTGAAGTGT TCCGTTGAAT 
     4201 ATCACACTGA AACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     4261 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4321 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4381 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4441 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4501 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4561 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4621 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4681 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     4741 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     4801 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     4861 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     4921 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     4981 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     5041 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     5101 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     5161 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     5221 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     5281 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     5341 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     5401 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     5461 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     5521 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     5581 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     5641 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     5701 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     5761 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     5821 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     5881 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     5941 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6001 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6061 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     6121 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     6181 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     6241 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6301 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     6361 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6421 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6481 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     6541 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     6601 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     6661 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     6721 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     6781 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     6841 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     6901 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     6961 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7021 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7081 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     7141 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     7201 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     7261 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7321 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7381 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7441 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7501 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     7561 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     7621 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     7681 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     7741 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     7801 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     7861 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     7921 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     7981 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     8041 TAACAAAAAT TTAACGCGAA TTTT
//