LOCUS       RC235144                8058 bp    DNA     circular  SYN 
DEFINITION  ALG13 (Myc-DDK tagged) - Homo sapiens ALG13, UDP-N-acetylglucosaminyltransferase subunit (ALG13), transcript variant 7
ACCESSION   NM_001257231
VERSION     NM_001257231.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4215..4306 
                     /label="Myc-DDK"  
     misc_feature    4336..4921 
                     /label="PolyA signal"  
     misc_feature    4393..4412 
                     /label="Restriction Enzyme"  
     misc_feature    5197..5869 
                     /label="ColE1"  
     misc_feature    6299..7093 
                     /label="Kan/Neo"  
     misc_feature    7128..7430 
                     /label="SV40 ori"  
     misc_feature    7516..7993 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4197..4203 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4197 
                     /label="ALG13"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTTTACAGGT GCAGGAAGCT GTTTGGAGAC TCTGGAAAAA GGAAAGCCAC 
     1081 TCGTAGTGGT TATAAACGAA AAGTTGATGA ACAATCATCA GCTGGAACTG GCAAAGCAGC 
     1141 TACACAAAGA GGGTCATCTC TTCTATTGTA CCTGCAGGGT CCTGACTTGT CCTGGGCAAG 
     1201 CCAAGTCCAT TGCTTCTGCT CCTGGGAAGT GCCAAGATTC TGCAGCGCTG ACTTCAACTG 
     1261 CCTTTTCAGG CCTAGACTTT GGGCTGCTTT CCGGATACCT GCATAAGCAA GCCCTTGTTA 
     1321 CTGCTACCCA TCCTACCTGC ACCCTGCTTT TTCCCTCTTG CCACGCTTTT TTTCCTCTCC 
     1381 CTCTTACCCC CACCCTGTAC AAAATGCATA AAGGATGGAA AAACTACTGC AGCCAGAAGT 
     1441 CTTTGAATGA GGCATCAATG GATGAATATT TAGGCAGCTT AGGGCTGTTT CGAAAGCTGA 
     1501 CTGCCAAGGA TGCCTCTTGC CTCTTTCGGG CCATTTCGGA GCAGTTGTTT TGCAGCCAGG 
     1561 TCCATCATTT GGAAATCAGG AAGGCTTGTG TCTCATATAT GAGGGAAAAT CAACAAACTT 
     1621 TTGAGTCTTA TGTGGAGGGA TCTTTTGAGA AATACCTGGA ACGGTTGGGA GATCCCAAGG 
     1681 AAAGTGCTGG CCAGCTGGAA ATAAGAGCTC TTTCTCTAAT TTATAATCGG GATTTCATTC 
     1741 TTTATCGCTT TCCTGGAAAA CCTCCAACTT ATGTCACAGA TAATGGCTAT GAAGACAAGA 
     1801 TTCTACTCTG CTACTCAAGT AGTGGTCACT ATGATTCTGT GTACTCAAAA CAATTTCAGT 
     1861 CAAGTGCAGC TGTTTGTCAG GCTGTATTGT ACGAAATTCT CTATAAAGAT GTGTTTGTTG 
     1921 TGGATGAAGA AGAGTTGAAG ACTGCGATTA AATTGTTTCG AAGTGGTTCT AAGAAGAACA 
     1981 GAAATAATGC TGTAACTGGA AGCGAGGATG CCCATACTGA TTACAAGAGT TCAAATCAGA 
     2041 ATAGGATGGA AGAGTGGGGT GCCTGCTACA ATGCTGAAAA TATACCAGAG GGCTACAATA 
     2101 AAGGAACAGA AGAAACAAAG TCTCCAGAAA ATCCATCAAA GATGCCCTTC CCCTATAAGG 
     2161 TGCTCAAAGC CCTGGATCCA GAAATCTATC GTAATGTAGA ATTTGATGTT TGGTTGGACA 
     2221 GCAGAAAAGA ACTTCAAAAA TCTGATTACA TGGAGTATGC TGGGAGACAG TACTATTTGG 
     2281 GAGACAAGTG TCAGGTTTGC TTGGAATCAG AAGGAAGATA TTATAATGCT CATATCCAGG 
     2341 AAGTTGGAAA TGAGAACAAT TCAGTAACAG TCTTCATTGA AGAATTGGCG GAAAAGCATG 
     2401 TTGTTCCACT GGCTAACTTA AAACCAGTTA CCCAAGTGAT GTCTGTTCCT GCCTGGAATG 
     2461 CTATGCCCAG TCGGAAAGGA AGAGGTTACC AGAAAATGCC TGGGGGTTAT GTCCCGGAAA 
     2521 TAGTTATATC AGAGATGGAC ATAAAGCAAC AGAAGAAGAT GTTCAAGAAA ATTCGAGGGA 
     2581 AAGAAGTTTA CATGACTATG GCTTACGGCA AGGGAGACCC CCTCCTCCCA CCCAGGCTGC 
     2641 AGCACAGTAT GCATTATGGG CACGATCCTC CAATGCACTA CTCACAGACA GCTGGCAATG 
     2701 TTATGTCTAA TGAACATTTT CATCCTCAGC ATCCATCTCC GAGACAAGGT CGGGGATATG 
     2761 GGATGCCCAG GAATTCATCT CGGTTTATAA ACAGGCACAA CATGCCGGGC CCTAAAGTTG 
     2821 ATTTTTACCC AGGCCCAGGT AAAAGGTGCT GCCAGAGCTA TGATAACTTC TCTTATAGAT 
     2881 CTCGTTCATT TAGACGTAGT CACCGCCAGA TGAGTTGTGT GAATAAGGAG TCCCAGTATG 
     2941 GATTTACCCC AGGGAATGGA CAGATGCCCA GGGGCTTGGA AGAAACTATT ACTTTTTATG 
     3001 AAGTTGAAGA AGGGGATGAG ACTGCTTATC CAACTTTACC TAATCATGGA GGTCCCTCTA 
     3061 CAATGGTTCC TGCTACTTCA GGATACTGTG TTGGAAGGCG GGGACATAGC TCAGGCAAAC 
     3121 AGACTTTGAA TTTAGAGGAG GGCAATGGCC AGAGTGAAAA TGGGCGATAT CATGAAGAAT 
     3181 ATCTTTATCG TGCAGAGCCA GACTATGAAA CTTCAGGTGT TTATAGCACA ACTGCATCTA 
     3241 CAGCAAACTT GTCTCTTCAG GACAGAAAGT CATGTTCTAT GTCTCCTCAG GACACAGTTA 
     3301 CCTCATACAA CTACCCCCAG AAGATGATGG GAAATATTGC AGCAGTTGCA GCTTCCTGTG 
     3361 CCAATAATGT TCCAGCTCCA GTCTTATCTA ACGGTGCAGC GGCTAATCAA GCTATTAGTA 
     3421 CCACTTCAGT TTCCTCACAG AATGCTATAC AGCCTCTCTT TGTATCTCCA CCTACACACG 
     3481 GCAGGCCAGT GATTGCCTCA CCATCCTATC CATGCCATTC TGCTATTCCT CATGCTGGTG 
     3541 CCTCTCTACC ACCACCACCA CCACCACCAC CACCACCACC ACCACCACCA CCTCCTCCTC 
     3601 CTCCTCCTCC TCCTCCTCCT CCTCCTCCTG CTCTTGATGT GGGAGAGACT TCAAACTTAC 
     3661 AACCACCACC ACCACTACCA CCTCCACCTT ATTCCTGTGA TCCAAGCGGC AGTGATTTGC 
     3721 CTCAAGATAC AAAAGTTTTG CAGTACTATT TCAATCTAGG ATTGCAGTGC TATTACCACA 
     3781 GCTACTGGCA CTCCATGGTC TATGTGCCAC AGATGCAGCA GCAGCTTCAT GTAGAGAATT 
     3841 ATCCAGTCTA TACTGAGCCA CCTCTGGTAG ATCAAACCGT TCCTCAATGC TACAGTGAGG 
     3901 TGAGGAGAGA AGATGGCATA CAGGCGGAAG CATCAGCAAA TGATACTTTT CCGAATGCTG 
     3961 ATTCTTCATC TGTCCCTCAT GGAGCAGTCT ATTATCCAGT AATGTCAGAT CCCTATGGGC 
     4021 AGCCACCTTT GCCAGGTTTT GACTCCTGCC TTCCGGTTGT GCCAGATTAT TCCTGTGTTC 
     4081 CCCCCTGGCA TCCAGTTGGT ACAGCATATG GTGGTTCTTC TCAAATTCAT GGTGCTATAA 
     4141 ATCCTGGGCC AATTGGCTGT ATTGCTCCAT CTCCCCCAGC TTCTCATTAT GTACCTCAGG 
     4201 GTATGACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     4261 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     4321 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4381 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     4441 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     4501 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     4561 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     4621 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     4681 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     4741 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     4801 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     4861 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     4921 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     4981 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     5041 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     5101 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     5161 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     5221 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     5281 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     5341 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     5401 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     5461 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     5521 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     5581 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     5641 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     5701 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     5761 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     5821 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     5881 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     5941 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     6001 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     6061 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     6121 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     6181 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     6241 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     6301 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     6361 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     6421 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     6481 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     6541 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     6601 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     6661 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     6721 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     6781 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     6841 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     6901 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     6961 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     7021 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     7081 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     7141 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     7201 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     7261 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     7321 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7381 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7441 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     7501 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     7561 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     7621 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     7681 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     7741 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     7801 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     7861 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     7921 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     7981 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     8041 AAATTTAACG CGAATTTT
//