LOCUS       RC235142                8052 bp    DNA     circular  SYN 
DEFINITION  SEMA5B (Myc-DDK tagged) - Homo sapiens sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B (SEMA5B), transcript variant 4
ACCESSION   NM_001256348
VERSION     NM_001256348.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4209..4300 
                     /label="Myc-DDK"  
     misc_feature    4330..4915 
                     /label="PolyA signal"  
     misc_feature    4387..4406 
                     /label="Restriction Enzyme"  
     misc_feature    5191..5863 
                     /label="ColE1"  
     misc_feature    6293..7087 
                     /label="Kan/Neo"  
     misc_feature    7122..7424 
                     /label="SV40 ori"  
     misc_feature    7510..7987 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4191..4197 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4191 
                     /label="SEMA5B"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTGCTTGCA GGCCCCCTGG CTGTCTCGCT GTTGCTGCCC AGCCTCACAC 
     1081 TGCTGGTGTC CCACCTCTCC AGCTCCCAGG ATGTCTCCAG TGAGCCCAGC AGTGAGCAGC 
     1141 AGCTGTGCGC CCTTAGCAAG CACCCCACCG TGGCCTTTGA AGACCTGCAG CCGTGGGTCT 
     1201 CTAACTTCAC CTACCCTGGA GCCCGGGATT TCTCCCAGCT GGCTTTGGAC CCCTCCGGGA 
     1261 ACCAGCTCAT CGTGGGAGCC AGGAACTACC TCTTCAGACT CAGCCTTGCC AATGTCTCTC 
     1321 TTCTTCAGGC CACAGAGTGG GCCTCCAGTG AGGACACGCG CCGCTCCTGC CAAAGCAAAG 
     1381 GGAAGACTGA GGAGGAGTGT CAGAACTACG TGCGAGTCCT GATCGTCGCC GGCCGGAAGG 
     1441 TGTTCATGTG TGGAACCAAT GCCTTTTCCC CCATGTGCAC CAGCAGACAG GTGGGGAACC 
     1501 TCAGCCGGAC TATTGAGAAG ATCAATGGTG TGGCCCGCTG CCCCTATGAC CCACGCCACA 
     1561 ACTCCACAGC TGTCATCTCC TCCCAGGGGG AGCTCTATGC AGCCACGGTC ATCGACTTCT 
     1621 CAGGTCGGGA CCCTGCCATC TACCGCAGCC TGGGCAGTGG GCCACCGCTT CGCACTGCCC 
     1681 AATATAACTC CAAGTGGCTT AATGAGCCAA ACTTCGTGGC AGCCTATGAT ATTGGGCTGT 
     1741 TTGCATACTT CTTCCTGCGG GAGAACGCAG TGGAGCACGA CTGTGGACGC ACCGTGTACT 
     1801 CTCGCGTGGC CCGCGTGTGC AAGAATGACG TGGGGGGCCG ATTCCTGCTG GAGGACACAT 
     1861 GGACCACATT CATGAAGGCC CGGCTCAACT GCTCCCGCCC GGGCGAGGTC CCCTTCTACT 
     1921 ATAACGAGCT GCAGAGTGCC TTCCACTTGC CGGAGCAGGA CCTCATCTAT GGAGTTTTCA 
     1981 CAACCAACGT AAACAGCATC GCGGCTTCTG CTGTCTGCGC CTTCAACCTC AGTGCTATCT 
     2041 CCCAGGCTTT CAATGGCCCA TTTCGCTACC AGGAGAACCC CAGGGCTGCC TGGCTCCCCA 
     2101 TAGCCAACCC CATCCCCAAT TTCCAGTGTG GCACCCTGCC TGAGACCGGT CCCAACGAGA 
     2161 ACCTGACGGA GCGCAGCCTG CAGGACGCGC AGCGCCTCTT CCTGATGAGC GAGGCCGTGC 
     2221 AGCCGGTGAC ACCCGAGCCC TGTGTCACCC AGGACAGCGT GCGCTTCTCA CACCTCGTGG 
     2281 TGGACCTGGT GCAGGCTAAA GACACGCTCT ACCATGTACT CTACATTGGC ACCGAGTCGG 
     2341 GCACCATCCT GAAGGCGCTG TCCACGGCGA GCCGCAGCCT CCACGGCTGC TACCTGGAGG 
     2401 AGCTGCACGT GCTGCCCCCC GGGCGCCGCG AGCCCCTGCG CAGCCTGCGC ATCCTGCACA 
     2461 GCGCCCGCGC GCTCTTCGTG GGGCTGAGAG ACGGCGTCCT GCGGGTCCCA CTGGAGAGGT 
     2521 GCGCCGCCTA CCGCAGCCAG GGGGCATGCC TGGGGGCCCG GGACCCGTAC TGTGGCTGGG 
     2581 ACGGGAAGCA GCAACGTTGC AGCACACTCG AGGACAGCTC CAACATGAGC CTCTGGACCC 
     2641 AGAACATCAC CGCCTGTCCT GTGCGGAATG TGACACGGGA TGGGGGCTTC GGCCCATGGT 
     2701 CACCATGGCA ACCATGTGAG CACTTGGATG GGGACAACTC AGGCTCTTGC CTGTGTCGAG 
     2761 CTCGATCCTG TGATTCCCCT CGACCCCGCT GTGGGGGCCT TGACTGCCTG GGGCCAGCCA 
     2821 TCCACATCGC CAACTGCTCC AGGAATGGGG CGTGGACCCC GTGGTCATCG TGGGCGCTGT 
     2881 GCAGCACGTC CTGTGGCATC GGCTTCCAGG TCCGCCAGCG AAGTTGCAGC AACCCTGCTC 
     2941 CCCGCCACGG GGGCCGCATC TGCGTGGGCA AGAGCCGGGA GGAACGGTTC TGTAATGAGA 
     3001 ACACGCCTTG CCCGGTGCCC ATCTTCTGGG CTTCCTGGGG CTCCTGGAGC AAGTGCAGCA 
     3061 GCAACTGTGG AGGGGGCATG CAGTCGCGGC GTCGGGCCTG CGAGAACGGC AACTCCTGCC 
     3121 TGGGCTGCGG CGTGGAGTTC AAGACGTGCA ACCCCGAGGG CTGCCCCGAA GTGCGGCGCA 
     3181 ACACCCCCTG GACGCCGTGG CTGCCCGTGA ACGTGACGCA GGGCGGGGCA CGGCAGGAGC 
     3241 AGCGGTTCCG CTTCACCTGC CGCGCGCCCC TTGCAGACCC GCACGGCCTG CAGTTCGGCA 
     3301 GGAGAAGGAC CGAGACGAGG ACCTGTCCCG CGGACGGCTC CGGCTCCTGC GACACCGACG 
     3361 CCCTGGTGGA GGTCCTCCTG CGCAGCGGGA GCACCTCCCC GCACACGGTG AGCGGGGGCT 
     3421 GGGCCGCCTG GGGCCCGTGG TCGTCCTGCT CCCGGGACTG CGAGCTGGGC TTCCGCGTCC 
     3481 GCAAGAGAAC GTGCACTAAC CCGGAGCCCC GCAACGGGGG CCTGCCCTGC GTGGGCGATG 
     3541 CTGCCGAGTA CCAGGACTGC AACCCCCAGG CTTGCCCAGG TGAGGACATC TGTCTCGGGC 
     3601 TGCACACGGA GGAGGCACTA TGTGCCACAC AGGCCTGCCC AGAAGGCTGG TCGCCCTGGT 
     3661 CTGAGTGGAG TAAGTGCACT GACGACGGAG CCCAGAGCCG AAGCCGGCAC TGTGAGGAGC 
     3721 TCCTCCCAGG GTCCAGCGCC TGTGCTGGAA ACAGCAGCCA GAGCCGCCCC TGCCCCTACA 
     3781 GCGAGATTCC CGTCATCCTG CCAGCCTCCA GCATGGAGGA GGCCACCGAC TGTGCAGGGT 
     3841 TCAATCTCAT CCACTTGGTG GCCACGGGCA TCTCCTGCTT CTTGGGCTCT GGGCTCCTGA 
     3901 CCCTAGCAGT GTACCTGTCT TGCCAGCACT GCCAGCGTCA GTCCCAGGAG TCCACACTGG 
     3961 TCCATCCTGC CACCCCCAAC CATTTGCACT ACAAGGGCGG AGGCACCCCG AAGAATGAAA 
     4021 AGTACACACC CATGGAATTC AAGACCCTGA ACAAGAATAA CTTGATCCCT GATGACAGAG 
     4081 CCAACTTCTA CCCATTGCAG CAGACCAATG TGTACACGAC TACTTACTAC CCAAGCCCCC 
     4141 TGAACAAACA CAGCTTCCGG CCCGAGGCCT CACCTGGACA ACGGTGCTTC CCCAACAGCA 
     4201 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     4261 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     4321 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     4381 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     4441 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     4501 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     4561 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     4621 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     4681 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     4741 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     4801 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     4861 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     4921 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     4981 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     5041 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     5101 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     5161 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     5221 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     5281 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     5341 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     5401 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     5461 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     5521 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     5581 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     5641 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     5701 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     5761 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     5821 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     5881 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     5941 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     6001 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     6061 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     6121 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     6181 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     6241 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     6301 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     6361 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     6421 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     6481 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     6541 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     6601 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     6661 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     6721 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     6781 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     6841 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     6901 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     6961 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     7021 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     7081 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     7141 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     7201 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     7261 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     7321 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     7381 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7441 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     7501 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     7561 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     7621 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     7681 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     7741 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     7801 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     7861 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     7921 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     7981 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     8041 AACGCGAATT TT
//