LOCUS       RC235100                7824 bp    DNA     circular  SYN 
DEFINITION  USP15 (Myc-DDK tagged) - Homo sapiens ubiquitin specific peptidase 15 (USP15), transcript variant 1
ACCESSION   NM_001252078
VERSION     NM_001252078.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3981..4072 
                     /label="Myc-DDK"  
     misc_feature    4102..4687 
                     /label="PolyA signal"  
     misc_feature    4159..4178 
                     /label="Restriction Enzyme"  
     misc_feature    4963..5635 
                     /label="ColE1"  
     misc_feature    6065..6859 
                     /label="Kan/Neo"  
     misc_feature    6894..7196 
                     /label="SV40 ori"  
     misc_feature    7282..7759 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3963..3969 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3963 
                     /label="USP15"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGAAGGC GGAGCGGCGG ATCTGGACAC CCAGCGGTCT GACATCGCGA 
     1081 CGCTGCTCAA AACCTCGCTC CGGAAAGGGG ACACCTGGTA CCTAGTCGAT AGTCGCTGGT 
     1141 TCAAACAGTG GAAAAAATAT GTTGGCTTTG ACAGTTGGGA CAAATACCAG ATGGGAGATC 
     1201 AAAATGTGTA TCCTGGACCC ATTGATAACT CTGGACTTCT CAAAGATGGT GATGCCCAGT 
     1261 CACTTAAGGA ACACCTTATT GATGAATTGG ATTACATACT GTTGCCAACT GAAGGTTGGA 
     1321 ATAAACTTGT CAGCTGGTAC ACATTGATGG AAGGTCAAGA GCCAATAGCA CGAAAGGTGG 
     1381 TTGAACAGGG TATGTTTGTA AAGCACTGCA AAGTAGAAGT ATATCTCACA GAATTGAAGC 
     1441 TATGTGAAAA TGGAAACATG AATAATGTTG TAACTCGAAG ATTTAGCAAA GCTGACACAA 
     1501 TAGATACAAT TGAAAAGGAA ATAAGAAAAA TCTTCAGTAT TCCAGATGAA AAGGAGACCA 
     1561 GATTGTGGAA CAAATACATG AGTAACACAT TTGAACCACT GAATAAACCA GACAGCACCA 
     1621 TTCAGGATGC TGGTTTATAC CAAGGACAGG TATTAGTGAT AGAACAGAAA AATGAAGATG 
     1681 GAACATGGCC AAGGGGTCCT TCTACTCCTA AGTCCCCAGG TGCATCCAAT TTTTCAACTT 
     1741 TACCAAAGAT CTCTCCTTCA TCTCTATCAA ATAATTATAA CAACATGAAC AACAGAAATG 
     1801 TGAAAAACTC AAATTACTGT CTTCCATCAT ATACCGCTTA TAAGAACTAT GATTATTCGG 
     1861 AACCTGGAAG AAACAATGAA CAGCCAGGCC TCTGTGGCCT AAGTAACTTG GGAAATACGT 
     1921 GTTTCATGAA CTCAGCTATT CAGTGTTTGA GCAACACACC TCCACTTACT GAGTATTTCC 
     1981 TCAATGATAA GTATCAAGAA GAACTGAATT TTGACAATCC CTTAGGAATG AGAGGTGAAA 
     2041 TAGCTAAATC TTATGCCGAA CTGATCAAGC AAATGTGGTC TGGAAAGTTT AGCTACGTCA 
     2101 CCCCAAGAGC CTTTAAGACA CAGGTAGGAC GTTTTGCACC TCAGTTCTCT GGATATCAGC 
     2161 AGCAAGACTG TCAAGAACTG TTAGCTTTCC TATTAGATGG ATTACATGAG GATTTGAATA 
     2221 GAATTAGGAA AAAACCATAT ATACAATTAA AAGATGCAGA TGGAAGGCCA GATAAGGTGG 
     2281 TTGCCGAAGA AGCCTGGGAA AACCATTTAA AACGAAATGA TTCTATCATA GTAGATATAT 
     2341 TTCATGGCCT TTTCAAATCA ACTTTAGTTT GTCCTGAGTG TGCTAAGATT TCAGTAACAT 
     2401 TTGATCCTTT TTGTTACTTG ACACTTCCAT TGCCCATGAA AAAAGAACGC ACCTTGGAAG 
     2461 TTTACTTAGT TAGAATGGAT CCACTTACCA AACCTATGCA GTACAAAGTG GTTGTCCCCA 
     2521 AAATTGGAAA CATATTAGAT CTTTGTACAG CATTGTCTGC TTTGTCAGGA ATACCTGCAG 
     2581 ATAAGATGAT AGTTACTGAT ATATACAATC ATAGATTTCA CAGAATATTC GCTATGGATG 
     2641 AAAACCTTAG TAGTATTATG GAACGGGATG ATATTTATGT GTTTGAAATT AACATCAATA 
     2701 GGACAGAAGA TACAGAGCAC GTGATTATTC CTGTTTGCCT AAGAGAAAAA TTCAGACACT 
     2761 CGAGTTATAC CCACCATACT GGTTCTTCAC TTTTTGGTCA GCCCTTTCTT ATGGCTGTAC 
     2821 CACGAAACAA TACTGAAGAC AAACTTTATA ATCTCCTGCT CTTGAGAATG TGCCGATATG 
     2881 TCAAAATATC TACTGAAACT GAAGAAACTG AAGGATCCCT ACACTGCTGT AAGGACCAAA 
     2941 ATATTAATGG GAATGGCCCA AATGGCATAC ATGAAGAAGG CTCACCAAGT GAAATGGAAA 
     3001 CAGATGAGCC AGATGATGAA TCCAGCCAGG ATCAAGAACT TCCCTCAGAG AATGAAAACA 
     3061 GTCAGTCTGA AGATTCAGTT GGAGGAGATA ATGATTCTGA AAATGGATTA TGTACTGAGG 
     3121 ATACTTGCAA AGGTCAACTC ACGGGACACA AAAAACGATT GTTTACATTC CAGTTCAACA 
     3181 ACTTAGGCAA TACTGATATC AACTACATCA AAGATGATAC CAGGCATATA AGATTTGATG 
     3241 ATAGGCAGCT TAGGCTAGAT GAAAGATCTT TTCTTGCTTT GGATTGGGAT CCTGATTTGA 
     3301 AAAAAAGATA TTTTGATGAA AATGCTGCTG AGGACTTTGA AAAACATGAA AGTGTGGAGT 
     3361 ATAAACCTCC TAAAAAACCC TTTGTGAAAT TAAAAGATTG CATTGAACTT TTTACAACAA 
     3421 AAGAAAAGCT AGGTGCTGAA GATCCCTGGT ATTGTCCGAA TTGTAAAGAA CATCAGCAAG 
     3481 CCACAAAGAA ATTGGATTTA TGGTCCCTGC CTCCAGTACT TGTAGTACAT CTCAAGCGAT 
     3541 TTTCTTACAG TCGATACATG AGAGACAAGT TGGATACCTT AGTTGATTTT CCTATCAATG 
     3601 ACTTGGATAT GTCGGAATTC TTAATTAATC CAAATGCAGG TCCTTGCCGC TATAATCTGA 
     3661 TTGCTGTTTC CAACCACTAT GGAGGGATGG GAGGAGGACA CTATACTGCT TTTGCAAAAA 
     3721 ATAAAGATGA TGGAAAATGG TACTATTTTG ATGACAGTAG TGTCTCCACT GCATCTGAAG 
     3781 ACCAAATTGT GTCCAAAGCA GCATATGTAC TCTTCTACCA GAGACAAGAC ACTTTCAGTG 
     3841 GAACTGGCTT TTTTCCTCTT GACCGAGAAA CTAAAGGTGC TTCAGCTGCC ACTGGCATCC 
     3901 CATTAGAAAG TGATGAAGAT AGCAATGATA ATGACAATGA TATAGAAAAT GAAAACTGTA 
     3961 TGCACACTAA CACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     4021 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4081 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4141 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4201 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4261 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4321 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4381 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4441 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     4501 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     4561 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     4621 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     4681 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     4741 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     4801 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     4861 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     4921 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     4981 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     5041 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     5101 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     5161 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     5221 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     5281 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     5341 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     5401 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     5461 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     5521 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     5581 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     5641 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     5701 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     5761 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     5821 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     5881 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     5941 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     6001 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6061 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     6121 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6181 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6241 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     6301 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     6361 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     6421 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     6481 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     6541 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     6601 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     6661 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     6721 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     6781 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     6841 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     6901 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     6961 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     7021 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7081 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7141 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7201 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7261 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     7321 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     7381 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     7441 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     7501 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     7561 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     7621 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     7681 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     7741 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     7801 TAACAAAAAT TTAACGCGAA TTTT
//